Information report for RNHL-A1;Rht8
Gene Details
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Functional Descriptions
- Designated ORF1 as RNHL-D1 and ORF2 as RNHL-D1.2. Two independent rnhl-d1 mutants showed significant reduction in plant height and spike length compared to the non-edited control. The RNHL-D1-overexpressing transgenic lines showed significantly increased plant height and SL.
- Suggesting a significant dosage effect of RNHL1 homoeologs in regulating plant height.
- Our phenotyping and genetic mapping analyses collectively suggested that these two newly identified EMS-induced mutants are allelic to Rht8, thus we renamed them Rht8-2 and Rht8-3,respectively. Rht8-2 and Rht8-3 reduced plant height and had no obvious negative impact on grain yield.
- A natural variation in Ribonuclease H-like gene underlies Rht8 to confer “Green Revolution” trait in wheat.
Functional Keywords
- grain , grain-length , grain-yield , yield , height , plant-height , spike
Literature and News
- A natural variation in Ribonuclease H-like gene underlies Rht8 to confer "Green Revolution" trait in wheat. DOI: 10.1016/j.molp.2022.01.013 ; PMID: 35063659
- Cloning and functional characterization of Rht8, a "Green Revolution" replacement gene in wheat. DOI: 10.1016/j.molp.2022.01.014 ; PMID: 35063661
Gene Resources
- NCBI ID: 123187181
- UniProt accessions: A0A3B6AR80
- AlphaFoldDB: A0A3B6AR80
- EnsemblPlants: TraesCS2A02G059900.1
- Gramene: TraesCS2A02G059900.1
- OMA: SKIKDWY
- InterPro: IPR012337
- PANTHER: PTHR32166 , PTHR32166:SF107
- SUPFAM: SSF53098
Sequences
cDNA Sequence
- >TraesCS2A02G059900.1
AACTCCCAAGATTCATTTACCTACAAGTATTGCCGGGCTCGGGGATCCAAGATAAATAGAGGGAGAGAGTGAGGAGGAGCTCCTCCTAGACGGACGGACCCTCCCTCTGTCTCTGCTTGTTTTTGGAGGGCCTTGGAGAAGAGAGCGAGGAGCGAGCGTTCGGGCGTGATATACTAGTCGGGCTACACGGGGGCCGAGATAAATCGGGGGGAGGAAAAAAGGGGGTGAAAATACGCCGCCCGCCCGCACCACTTCTCCTCAGGCCTTGACGCGGCCAGCAGCCCCTCCAGCCGGGCGATAGTTGGAAAGGAGAGCGAAACGGGCGCCCGAAACCCCCTGAAAACCTCCACGAGCCACGCCCCCGCCCATGTCGGCGCCGGAGGAGGGCGACGCCGCCGCCGCCGCCGCCCCGGGATCGTCGGCGCCGCCCGCGCCCGCGCCCGCGCCGCCGCCGATGCCGGAGCCCGGCGCAATGGGGCCCGAGGAGGCCGCCGCCAGGAAGCGCTACGAGGCGCTGATGCAGGTGCGGGCCAAGGCGCTCAAGGGGAAGGGCGCCTGGTACTGGGGCCACCTCGAGCCCGTCCTCGTCCCGCCGCCGGCCTCCGGGGCGCCCCCCAAGGCCGCCCGCCTCCGATGCGCGCTCTGCGCCGCCACCTTCTCCGCCTCCAACCCCTCACGCACCGCCACCGAGCACCTCAAGCGCGGCGCCTGCCCTAATTTCGCGGCGGCGCAGGGCGCCCCGCCGCCGCCGCCGCCCCCGCCCCCGCCGCAGAATCAGCATCAGCAGTTGCAGCTCGTCGCCGTCTCCTCCCCCGCCTCCTCGATCGTGCCCATCTCCTCCATCCCCCCGTCGTCCTCGTCCTCCTCGCAGCGCCGCCACTCCACCGGCGGCGGCGGCCGGAAGCGGCACGCGCTCGCCGCGGCGTACGCGTCCGTCGAGGCGGCCTCGCACCAGCACCACCTCATGGTCGCCGACCCGGCCGGGTACTCCCCGACGCCGCCCACCCCGCCGGGGATGCCCGCGCCCAGGCAGCTGCTCTCCGGCGGCCGGGGCGACCTCGCCCCGCTCGCCATGCTGGAGGACAGCGTCAAGCGCCTCAAGTCGCCCTCGGCGTCGCCGGGCGCGATGCTTCCGCGGCCGCAGGCCGAGGCCGCGCTCGCGCTGCTCGCCGACTGGTTCCTCGAGTCGTCCGGCAGCGCCTCCCTCTCCGCGGTCGAGCACCCCAAGCTGAAGAACTTCCTGCGCCAGGTCGGGCTCCCGGAGATATCGCGAGCCGACCTCGCCGGCGCGCGCCTGGACGCGCGCTTCGCCGAGGCCCGCGCCGACGCCGCCGCGCGCTTCCGCGAGGCGCGCTTCTTCCAGCTCGCGGCCGACGGCTTGCGGGAGCAGGTGATCACCCTCTCCGTCAACCTCCCCAACGACACGTCGGTGTTCCACCGCGCCGTGCCCATGCCCGCGCCGGCGTCGGCGTCCCCTGACTACGCCCAGGAGCTGCTGCTGGACGCGGCATCGTCCGTCTCCGCCTCCTCCGGCGACATTCGGCATTGCGCCGGCATCGTCGCCGACCGCTTCGGCTCCAAGACTCTGCGTGATCTCGAGACCAAGCACCATTGGATGGTGAACCTTACCTGCCAAGTCCATGGCCTCTCCCGCTTGGTAAGCGACATGGCGCGCGAGCTCCCACTCTTCAACAACGCTGCGTCAAATTGCGCCAAGATCGCCAGCTACTTCAACACCACGCCCTCCGTGCGCGCGCTGCTGCACAAGCACCAAGTCCAGGAGCACGGGCACGCCTTCCTCCTCCCCATTGCCGCTCCGCCGTATAATGGCGGGGAATTTGCCGCCGCGTTCGTGATGCTCGAGAGCATCCTGACCTCGGCGCGGCCGCTCCAGCTCGCCGTGCTCGAGGAGTCCTACAAGGTGGTCTGCATCGACGACCCTGCCGCGAGGGAGATCGCGGCCATGGTGCAGAATGTGGCGTTCTGGACCGAGGTCGAGGCGACGCATTCGGTCGTGAAGCTGATCATGGACTTGGTGAAGGAGATGGAGACCGAGAGGCCCCTGGTGGGGCAATGCCTGCCTCTCTGGGAGGACCTGCGCGGCAAGGTCAGAGGCTGGTGCCGGAAATTCAGCATAGAAGAGGCGACTGCCATGAATGTGGTGGAGAGGAGGTTCAGGAAGAACTACCACCCGGCGTGGTCAGCGGCATTCATACTGGACCCATTGTATCTGATCAAGGACGCCGGCAGGAGGTACCTTCCACCGTTCAACTACTTGACACCAGAGCAGGAGAAGGACGTGGACAGGCTGATCACAAGACTGGTGTCGCCGGAGGAGGCGCACCTCGCAGTGATGGAGCTGATGAAATGGCGGTCCGAGGGGCTCGACCCATTGTACGCGCAGGCGGTGCAAGTCCGGCAGCCAGACCCATCGACGGGGAAGATGAAGATAGCCAACAAGCAGAGCAGCCGGCTTGTGTGGGAGACATGCCTCAGCGAGTTCAAGTCGCTTGGCAAAGTGGCGGTCAGGCTCATCTTCCTCCATGCAACCGCCAAGGGGTTCAAATGCACGCCGTCGATGACGCGGTGGCTCACCGCGCCAGGCAGCTCAGCCGGCAGCATTGGCCGGGCGCACCGGCTGGTGTTCATCGCGGCGAACTCGAAGCTGGAGAGGAGGGATTTCTCAAACGACGACGACAAGGACGTGGAGCTGCTGACGGAAGGAGACGACGACATGCTAACAGAGACTGGCAATGTGGATCCCTCCTCCTCCTCAGTGTAGCATTCCATTCCTGCTGCTGCTGCTGCTGCTTAATTTCCTTGGCCTCTTTGTGCATTTGATGTTAGCATCCTTAGAATTTGATCTCTAGTAGGAAATTACTATATTCTTTTTTTGATTGTGCCCCCCATGTTGCTAGTTCTTGCTCCCTCCTCTTTGTTTTGGTTGGGTAGGGGGTCGGGGCATGAGATGGAGATATGCTGCACCCAGTCGGTCTTAATTTGCTTGTCAACTGTGGTTAATTAATAAATGGCTCCCTTGTC
CDS Sequence
- >TraesCS2A02G059900.1
ATGTCGGCGCCGGAGGAGGGCGACGCCGCCGCCGCCGCCGCCCCGGGATCGTCGGCGCCGCCCGCGCCCGCGCCCGCGCCGCCGCCGATGCCGGAGCCCGGCGCAATGGGGCCCGAGGAGGCCGCCGCCAGGAAGCGCTACGAGGCGCTGATGCAGGTGCGGGCCAAGGCGCTCAAGGGGAAGGGCGCCTGGTACTGGGGCCACCTCGAGCCCGTCCTCGTCCCGCCGCCGGCCTCCGGGGCGCCCCCCAAGGCCGCCCGCCTCCGATGCGCGCTCTGCGCCGCCACCTTCTCCGCCTCCAACCCCTCACGCACCGCCACCGAGCACCTCAAGCGCGGCGCCTGCCCTAATTTCGCGGCGGCGCAGGGCGCCCCGCCGCCGCCGCCGCCCCCGCCCCCGCCGCAGAATCAGCATCAGCAGTTGCAGCTCGTCGCCGTCTCCTCCCCCGCCTCCTCGATCGTGCCCATCTCCTCCATCCCCCCGTCGTCCTCGTCCTCCTCGCAGCGCCGCCACTCCACCGGCGGCGGCGGCCGGAAGCGGCACGCGCTCGCCGCGGCGTACGCGTCCGTCGAGGCGGCCTCGCACCAGCACCACCTCATGGTCGCCGACCCGGCCGGGTACTCCCCGACGCCGCCCACCCCGCCGGGGATGCCCGCGCCCAGGCAGCTGCTCTCCGGCGGCCGGGGCGACCTCGCCCCGCTCGCCATGCTGGAGGACAGCGTCAAGCGCCTCAAGTCGCCCTCGGCGTCGCCGGGCGCGATGCTTCCGCGGCCGCAGGCCGAGGCCGCGCTCGCGCTGCTCGCCGACTGGTTCCTCGAGTCGTCCGGCAGCGCCTCCCTCTCCGCGGTCGAGCACCCCAAGCTGAAGAACTTCCTGCGCCAGGTCGGGCTCCCGGAGATATCGCGAGCCGACCTCGCCGGCGCGCGCCTGGACGCGCGCTTCGCCGAGGCCCGCGCCGACGCCGCCGCGCGCTTCCGCGAGGCGCGCTTCTTCCAGCTCGCGGCCGACGGCTTGCGGGAGCAGGTGATCACCCTCTCCGTCAACCTCCCCAACGACACGTCGGTGTTCCACCGCGCCGTGCCCATGCCCGCGCCGGCGTCGGCGTCCCCTGACTACGCCCAGGAGCTGCTGCTGGACGCGGCATCGTCCGTCTCCGCCTCCTCCGGCGACATTCGGCATTGCGCCGGCATCGTCGCCGACCGCTTCGGCTCCAAGACTCTGCGTGATCTCGAGACCAAGCACCATTGGATGGTGAACCTTACCTGCCAAGTCCATGGCCTCTCCCGCTTGGTAAGCGACATGGCGCGCGAGCTCCCACTCTTCAACAACGCTGCGTCAAATTGCGCCAAGATCGCCAGCTACTTCAACACCACGCCCTCCGTGCGCGCGCTGCTGCACAAGCACCAAGTCCAGGAGCACGGGCACGCCTTCCTCCTCCCCATTGCCGCTCCGCCGTATAATGGCGGGGAATTTGCCGCCGCGTTCGTGATGCTCGAGAGCATCCTGACCTCGGCGCGGCCGCTCCAGCTCGCCGTGCTCGAGGAGTCCTACAAGGTGGTCTGCATCGACGACCCTGCCGCGAGGGAGATCGCGGCCATGGTGCAGAATGTGGCGTTCTGGACCGAGGTCGAGGCGACGCATTCGGTCGTGAAGCTGATCATGGACTTGGTGAAGGAGATGGAGACCGAGAGGCCCCTGGTGGGGCAATGCCTGCCTCTCTGGGAGGACCTGCGCGGCAAGGTCAGAGGCTGGTGCCGGAAATTCAGCATAGAAGAGGCGACTGCCATGAATGTGGTGGAGAGGAGGTTCAGGAAGAACTACCACCCGGCGTGGTCAGCGGCATTCATACTGGACCCATTGTATCTGATCAAGGACGCCGGCAGGAGGTACCTTCCACCGTTCAACTACTTGACACCAGAGCAGGAGAAGGACGTGGACAGGCTGATCACAAGACTGGTGTCGCCGGAGGAGGCGCACCTCGCAGTGATGGAGCTGATGAAATGGCGGTCCGAGGGGCTCGACCCATTGTACGCGCAGGCGGTGCAAGTCCGGCAGCCAGACCCATCGACGGGGAAGATGAAGATAGCCAACAAGCAGAGCAGCCGGCTTGTGTGGGAGACATGCCTCAGCGAGTTCAAGTCGCTTGGCAAAGTGGCGGTCAGGCTCATCTTCCTCCATGCAACCGCCAAGGGGTTCAAATGCACGCCGTCGATGACGCGGTGGCTCACCGCGCCAGGCAGCTCAGCCGGCAGCATTGGCCGGGCGCACCGGCTGGTGTTCATCGCGGCGAACTCGAAGCTGGAGAGGAGGGATTTCTCAAACGACGACGACAAGGACGTGGAGCTGCTGACGGAAGGAGACGACGACATGCTAACAGAGACTGGCAATGTGGATCCCTCCTCCTCCTCAGTGTAG
Protein Sequence
- >TraesCS2A02G059900.1.cds1
MSAPEEGDAAAAAAPGSSAPPAPAPAPPPMPEPGAMGPEEAAARKRYEALMQVRAKALKGKGAWYWGHLEPVLVPPPASGAPPKAARLRCALCAATFSASNPSRTATEHLKRGACPNFAAAQGAPPPPPPPPPPQNQHQQLQLVAVSSPASSIVPISSIPPSSSSSSQRRHSTGGGGRKRHALAAAYASVEAASHQHHLMVADPAGYSPTPPTPPGMPAPRQLLSGGRGDLAPLAMLEDSVKRLKSPSASPGAMLPRPQAEAALALLADWFLESSGSASLSAVEHPKLKNFLRQVGLPEISRADLAGARLDARFAEARADAAARFREARFFQLAADGLREQVITLSVNLPNDTSVFHRAVPMPAPASASPDYAQELLLDAASSVSASSGDIRHCAGIVADRFGSKTLRDLETKHHWMVNLTCQVHGLSRLVSDMARELPLFNNAASNCAKIASYFNTTPSVRALLHKHQVQEHGHAFLLPIAAPPYNGGEFAAAFVMLESILTSARPLQLAVLEESYKVVCIDDPAAREIAAMVQNVAFWTEVEATHSVVKLIMDLVKEMETERPLVGQCLPLWEDLRGKVRGWCRKFSIEEATAMNVVERRFRKNYHPAWSAAFILDPLYLIKDAGRRYLPPFNYLTPEQEKDVDRLITRLVSPEEAHLAVMELMKWRSEGLDPLYAQAVQVRQPDPSTGKMKIANKQSSRLVWETCLSEFKSLGKVAVRLIFLHATAKGFKCTPSMTRWLTAPGSSAGSIGRAHRLVFIAANSKLERRDFSNDDDKDVELLTEGDDDMLTETGNVDPSSSSV