Gene Details:

Functional Descriptions:

  • By genetic mapping and comparative sequence analyses, we identify Rf1 and Rf3 candidates that can restore normal pollen production in transgenic wheat plants carrying T-CMS.
  • We show that Rf1 and Rf3 bind to the mitochondrial orf279 transcript and induce cleavage, preventing expression of the CMS trait.
  • Rf1 and Rf3 genes that restore fertility of wheat plants carrying Triticum timopheevii-type cytoplasmic male sterility (T-CMS).
  • Expression of Rf1 and Rf3 candidates in anthers during pollen formation.
  • Testing the restoring capabilities of Rf1 and Rf3 candidate genes by transgenesis.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >TraesCS1A02G031600.1
    ATGTCGCCACCACGCCTCCGCCTCCTCCTCCTCCTCCTCCTCCTCCTCTGCGCCCGCCACTCCTCCTCCACCTCTCATCCCTCACGCATCTGGGATCCCCACGCCGCCTTCGCCGCCGCGACGGAGCGGGCGCGCTCTGGTAGGCTCACCACGGAGGACGCACACCACCTGTTCGACGAATTGCTGCGGCAGGGCAATCCTGTCCAGGAGCGTCCCTTGAATAACTTTCTGGCTGCCCTCGCCCGCGCGCCCGCGTCTGCATCCTGCAGCGATGGCCCCGCCCTGGCCGTCGCCCTCTTCGGCCGTTTGTCCCGAGGCGCCGGGCAACGGGTGGCGCAGCCAAATGTGTTCACCTATGGCGTCCTCATGGACTGCTGCTGCCGCGCTTGCCGCACAGATCTGGCGCTCGCCTTCTTTGGCCGTCTTCTCAGGACGGGCCTGCAGGCAGGCCAAGTCGTCTTCAACACCCTCCTCAAGGGCCTTTGCCACACAAAGCGGGCGGATGAGGCTCTGGACGTGCTTCTTCACAGGATGCCTGAGCTGGGCTGCACCCCCGATGTGGTGGCGTATAACACCGTCATCCATGGCTTCTTTAGGGAAGGCCAAGTAGGCAAGGCATGCGATATGTTCCATGGAATGGCACAGAAGGGTGTTATGCCTAATGTGGTGACATATAACTCGGTTATTGATGGGCTGTGCAAGGCCAGGGCCATGGACAAAGCAGAGACGGTCCTTAGTCAAATGGTAAATGATGGTGTCCGACCTGATAACGTGACATATAATAGTCTCATTCATGGATATTCCTCTTTGGGCAATTGGAAGGAGGCGGTTAGGGTATTCAAAGAGATGAGTCGGAGGTTTACACCAGATGTCATTACCTGCACCTCACTCATGGCCTTCCTTTGCAAGAATGGAAAAAGCAAAGAAGCTGCAGAAATTTTTGATTCAATGGCTATGAAGGGCCTGAAACGTGACACCGTTTCATATGCTATTCTCCTTCATGGGTATGCCACTGAAGGATGCTTGGTTGACATGATTAATCTCTTCAATTCAATGGCAACAGACTGTATTCTACCTAACTGTCATATCTTCAATATACTGATTAATGCATATGCTAAATCTGGCATGATGGGTGAGGCCATGCTTATATTCAAAAAAATGCACGAACAAGGAGTGAGTCCAGATGTAGTCACCTATTCAACTGTAATACATGCATTTTGCAAAATGGGTAGCTTGGACATTGCTATGGACAAATTTAACCAGATGGTTGATATGGGAGTACGACCGAATGCAATTGTTTATCATTCCCTAATCCAGGGTTTTTGTACACATGGGGATTTAGTGAAAGCAAAGGAATTGGTTACTGAAATGAGGAACAAAGCTATGCGTCCTCCTGATATTAAGATCTTCCATTCAATAATGAAGAACCTATGCACGGAAGGAAGGGTAATAGAAGCACGGGATATCCTCGACTTGATGGTGCACATAGGTATGAGGCCTGATGTTCCCATCTTTAATTTACTGATTGGTGGATACTGCCTAGTCCACAAGATGGAGGATGCAACAAAAATGTTTGATGATATGGTGTCATATGGTTTAGAACCTTGTAACAGTACGTATGGTATACTTATTAATGGCTATTGCAAAAACGGAAGGATTGATGACGGGCTGAATCTGTTCAAAGAAATGTTGCGCAAGGGACTTAAACCTACAACTTTTAATTACAACGTCATACTGGATGGATTATTTCTGGCTGGACGAACTGTTGCTGCAAAGGAAAAGTTTGATGAGATGGTTGAATCTGGAGTAAGTGTGTGCATTGATACATATTCGATAGTTCTTGGTGGACTTTGTAGAAATAATTGCACCGGTGAAGCAATCACGCTGTCCAAGAAATTAAGTACAATGAATGTCAAATTCAATATTGCAATTGTCAATATCTTGATTGGTGCATTCTTCAGGGTTCGACGAAACCAAGAAGCTAAGGATTTGTTTGCCGCAATACCAGCCAATGGCTTGGTAGCTAATGTTGTTACCTACAGCATGATGATGATAAATCTTATAAAAGAAGGGTCACTGGAAGAAGCTGACGATCTCTTTTTATCAATGGAGAAGAGCGGCTGTACCGCTGACTCTTGTATGTTAAATCATATCATCAGAAGGCTACTGGAAAGAGGAGAGATAGTCAAGGCTGGAAATTATATGTCGAAAGTTGATGCAAAGAGCTACTCACTTGAAGCTAAAACTGTTTCGCTGCTGATCTCCCTCTTTTTAGGGAAAGGGAGATATAGAGAACACATGAAATTGCTCCCTATGAAGTATCAGTTTCTCGAAGAAGCAGCCACAGTTGAA
CDS Sequence
  • >TraesCS1A02G031600.1
    ATGTCGCCACCACGCCTCCGCCTCCTCCTCCTCCTCCTCCTCCTCCTCTGCGCCCGCCACTCCTCCTCCACCTCTCATCCCTCACGCATCTGGGATCCCCACGCCGCCTTCGCCGCCGCGACGGAGCGGGCGCGCTCTGGTAGGCTCACCACGGAGGACGCACACCACCTGTTCGACGAATTGCTGCGGCAGGGCAATCCTGTCCAGGAGCGTCCCTTGAATAACTTTCTGGCTGCCCTCGCCCGCGCGCCCGCGTCTGCATCCTGCAGCGATGGCCCCGCCCTGGCCGTCGCCCTCTTCGGCCGTTTGTCCCGAGGCGCCGGGCAACGGGTGGCGCAGCCAAATGTGTTCACCTATGGCGTCCTCATGGACTGCTGCTGCCGCGCTTGCCGCACAGATCTGGCGCTCGCCTTCTTTGGCCGTCTTCTCAGGACGGGCCTGCAGGCAGGCCAAGTCGTCTTCAACACCCTCCTCAAGGGCCTTTGCCACACAAAGCGGGCGGATGAGGCTCTGGACGTGCTTCTTCACAGGATGCCTGAGCTGGGCTGCACCCCCGATGTGGTGGCGTATAACACCGTCATCCATGGCTTCTTTAGGGAAGGCCAAGTAGGCAAGGCATGCGATATGTTCCATGGAATGGCACAGAAGGGTGTTATGCCTAATGTGGTGACATATAACTCGGTTATTGATGGGCTGTGCAAGGCCAGGGCCATGGACAAAGCAGAGACGGTCCTTAGTCAAATGGTAAATGATGGTGTCCGACCTGATAACGTGACATATAATAGTCTCATTCATGGATATTCCTCTTTGGGCAATTGGAAGGAGGCGGTTAGGGTATTCAAAGAGATGAGTCGGAGGTTTACACCAGATGTCATTACCTGCACCTCACTCATGGCCTTCCTTTGCAAGAATGGAAAAAGCAAAGAAGCTGCAGAAATTTTTGATTCAATGGCTATGAAGGGCCTGAAACGTGACACCGTTTCATATGCTATTCTCCTTCATGGGTATGCCACTGAAGGATGCTTGGTTGACATGATTAATCTCTTCAATTCAATGGCAACAGACTGTATTCTACCTAACTGTCATATCTTCAATATACTGATTAATGCATATGCTAAATCTGGCATGATGGGTGAGGCCATGCTTATATTCAAAAAAATGCACGAACAAGGAGTGAGTCCAGATGTAGTCACCTATTCAACTGTAATACATGCATTTTGCAAAATGGGTAGCTTGGACATTGCTATGGACAAATTTAACCAGATGGTTGATATGGGAGTACGACCGAATGCAATTGTTTATCATTCCCTAATCCAGGGTTTTTGTACACATGGGGATTTAGTGAAAGCAAAGGAATTGGTTACTGAAATGAGGAACAAAGCTATGCGTCCTCCTGATATTAAGATCTTCCATTCAATAATGAAGAACCTATGCACGGAAGGAAGGGTAATAGAAGCACGGGATATCCTCGACTTGATGGTGCACATAGGTATGAGGCCTGATGTTCCCATCTTTAATTTACTGATTGGTGGATACTGCCTAGTCCACAAGATGGAGGATGCAACAAAAATGTTTGATGATATGGTGTCATATGGTTTAGAACCTTGTAACAGTACGTATGGTATACTTATTAATGGCTATTGCAAAAACGGAAGGATTGATGACGGGCTGAATCTGTTCAAAGAAATGTTGCGCAAGGGACTTAAACCTACAACTTTTAATTACAACGTCATACTGGATGGATTATTTCTGGCTGGACGAACTGTTGCTGCAAAGGAAAAGTTTGATGAGATGGTTGAATCTGGAGTAAGTGTGTGCATTGATACATATTCGATAGTTCTTGGTGGACTTTGTAGAAATAATTGCACCGGTGAAGCAATCACGCTGTCCAAGAAATTAAGTACAATGAATGTCAAATTCAATATTGCAATTGTCAATATCTTGATTGGTGCATTCTTCAGGGTTCGACGAAACCAAGAAGCTAAGGATTTGTTTGCCGCAATACCAGCCAATGGCTTGGTAGCTAATGTTGTTACCTACAGCATGATGATGATAAATCTTATAAAAGAAGGGTCACTGGAAGAAGCTGACGATCTCTTTTTATCAATGGAGAAGAGCGGCTGTACCGCTGACTCTTGTATGTTAAATCATATCATCAGAAGGCTACTGGAAAGAGGAGAGATAGTCAAGGCTGGAAATTATATGTCGAAAGTTGATGCAAAGAGCTACTCACTTGAAGCTAAAACTGTTTCGCTGCTGATCTCCCTCTTTTTAGGGAAAGGGAGATATAGAGAACACATGAAATTGCTCCCTATGAAGTATCAGTTTCTCGAAGAAGCAGCCACAGTTGAA
Protein Sequence
  • >TraesCS1A02G031600.1.cds1
    MSPPRLRLLLLLLLLLCARHSSSTSHPSRIWDPHAAFAAATERARSGRLTTEDAHHLFDELLRQGNPVQERPLNNFLAALARAPASASCSDGPALAVALFGRLSRGAGQRVAQPNVFTYGVLMDCCCRACRTDLALAFFGRLLRTGLQAGQVVFNTLLKGLCHTKRADEALDVLLHRMPELGCTPDVVAYNTVIHGFFREGQVGKACDMFHGMAQKGVMPNVVTYNSVIDGLCKARAMDKAETVLSQMVNDGVRPDNVTYNSLIHGYSSLGNWKEAVRVFKEMSRRFTPDVITCTSLMAFLCKNGKSKEAAEIFDSMAMKGLKRDTVSYAILLHGYATEGCLVDMINLFNSMATDCILPNCHIFNILINAYAKSGMMGEAMLIFKKMHEQGVSPDVVTYSTVIHAFCKMGSLDIAMDKFNQMVDMGVRPNAIVYHSLIQGFCTHGDLVKAKELVTEMRNKAMRPPDIKIFHSIMKNLCTEGRVIEARDILDLMVHIGMRPDVPIFNLLIGGYCLVHKMEDATKMFDDMVSYGLEPCNSTYGILINGYCKNGRIDDGLNLFKEMLRKGLKPTTFNYNVILDGLFLAGRTVAAKEKFDEMVESGVSVCIDTYSIVLGGLCRNNCTGEAITLSKKLSTMNVKFNIAIVNILIGAFFRVRRNQEAKDLFAAIPANGLVANVVTYSMMMINLIKEGSLEEADDLFLSMEKSGCTADSCMLNHIIRRLLERGEIVKAGNYMSKVDAKSYSLEAKTVSLLISLFLGKGRYREHMKLLPMKYQFLEEAATVE