Gene Details:

• Gene ID: TraesCS1D02G058900
• Gene Symbol: Pm24 Pm24b WTK3 MlHLT
• Gene Name: TANDEM KINASE 3
• Genome: Chinese_Spring1.0
• Species: Triticum aestivum

Functional Descriptions:

• Here, we report the map-based cloning of powdery mildew resistance gene Pm24 from Chinese wheat landrace Hulutou. It encodes a tandem kinase protein (TKP) with putative kinase-pseudokinase domains, designated WHEAT TANDEM KINASE 3 (WTK3,MK950855).
• Haplotype analysis revealed that a rare 6-bp natural deletion of lysine-glycine codons, endemic to wheat landraces of Shaanxi Province, China, in the kinase I domain (Kin I) of WTK3 is critical for the resistance function. Transgenic assay of WTK3 chimeric variants revealed that only the specific two amino acid deletion, rather than any of the single or more amino acid deletions, in the Kin I of WTK3 is responsible for gaining the resistance function of WTK3 against the Bgt fungus.
• The resistance function of Pm24 was validated by transgenic assay, independent mutants, and allelic association analyses.
• The 6-bp deletion in the fifth exon of WTK3 is critical for the powdery mildew resistance of Pm24.

Function-related keywords:

• kinase ,  resistance ,  map-based-cloning ,  protein-kinase ,  powdery-mildew ,  mildew-resistance ,  mildew ,  powdery-mildew-resistance

Literature:

• A rare gain of function mutation in a wheat tandem kinase confers resistance to powdery mildew.   DOI:  10.1038/s41467-020-14294-0 ;  PMID:  32015344

Related News:

• Nature Com | 遗传发育所刘志勇组发现新的小麦抗白粉病基因！

Gene Resources:

• NCBI ID: MK950855
• UniProt accessions: A0A3B5ZNU5

Sequences: