Gene Details:

Functional Descriptions:

  • We developed new Pinb2 allele specific markers and demonstrated their usefulness by identifying new Pinb2 alleles in Triticeae species performing an association analysis of Pinb2 with kernel hardness.
  • Pinb and Pinb2 loci with kernel hardness phenotypes was determined by a Chi-square test of independence.
  • Using the high-quality bread wheat reference genome, we show that PINB2 are encoded by three homoeologous loci Pinb2 not syntenic to the Hardness locus, with Pinb2-7A locus containing three tandem copies.
  • PINB2 proteins have several features conserved for the Pin/Pinb2 phylogenetic cluster but lack a structural basis of significant impact on kernel hardness. Pinb2 are seed-specifically expressed with varied expression levels between the homoeologous copies and among wheat varieties.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >TraesCS7A02G514400.1
    ATGAAGACCTTATTCCTCCTAGCTCTCCTTTCTCTTGTAGTGAGCACAACCATTGCGCAATACAAAGAGGTTGGCGGCAGCTATGAGAACGGTGGCGGAGGGTTTGGTTCTCAAAACTGCCCACCGGAGAAGACGAAGCTGGACTCTTGCAAGGATTATGTGATGGAGTGGTGCTTGGCGGTGAAGGGTTTTTCAATCGCCAGGCTTTTGAAATGGTGGAAGGGCGCCTGTGAACAAGAGGCCCTAGACCAGTGTTGCCAGCAACTACGCCCGATAGCAAAGAAGTGCCGTTGTGAGGCCATTTGGAGAGCCGTCCAAGGCAACCTTGGTGGCATCTTTGGTTTTCAGCAAGGCCAGATAACCAAACAAATTCAGAGGGCCATGATGTTGCCGTCCAAATGCAAATTGGATTCCAACTGCAAGTTCGTCGCTACTAATGGCTATTACTAGTGA
CDS Sequence
  • >TraesCS5D02G004100.1
    ATGAAGGCCCTCTTCCTCATAGGACTGCTTGCTCTGGTAGCGAGCACCGCCTTTGCGCAATATAGCGAAGTTGTTGGCAGTTACGATGTTGCTGGCGGGGGTGGTGCTCAACAATGCCCTGTAGAGACAAAGCTAAATTCATGCAGGAATTACCTGCTAGATCGATGCTCAACGATGAAGGATTTCCCGGTCACCTGGCGTTGGTGGAAATGGTGGAAGGGAGGTTGTCAAGAGCTCCTTGGGGAGTGTTGCAGTCGGCTCGGCCAAATGCCACCGCAATGCCGCTGCAACATCATCCAGGGGTCAATCCAAGGCGATCTCGGTGGCATCTTCGGATTTCAGCGTGATCGGGCAAGCAAAGTGATACAAGAAGCCAAGAACCTGCCGCCCAGGTGCAACCAGGGCCCTCCCTGCAACATCCCCGGCACTATTGGCTATTACTGGTGA
Protein Sequence
  • >TraesCS5D02G004100.1.cds1
    MKALFLIGLLALVASTAFAQYSEVVGSYDVAGGGGAQQCPVETKLNSCRNYLLDRCSTMKDFPVTWRWWKWWKGGCQELLGECCSRLGQMPPQCRCNIIQGSIQGDLGGIFGFQRDRASKVIQEAKNLPPRCNQGPPCNIPGTIGYYW