Gene Details:

Functional Descriptions:

  • Here, we report on the cloning of a wheat gene, ALMT1,that co-segregates with Al tolerance in F2 and F3 populations derived from crosses between near-isogenic wheat lines that differ in Al tolerance.
  • The ALMT1 gene encodes a membrane protein, which is constitutively expressed in the root apices of the Al-tolerant line at greater levels than in the near-isogenic but Al-sensitive line.
  • ALMT1 increased the tolerance of tobacco cells to Al treatment. These findings demonstrate that ALMT1 encodes an Al-activated malate transporter that is capable of conferring Al tolerance to plant cells.
  • Taken together, these results indicate that both ALMT1-1 and ALMT1-2 genes encode Al-activated transporters that facilitate malate efflux from tobacco cells, and that the expression of both ALMT1 genes increases Al tolerance by decreasing the accumulation of Al in cells.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >TraesCS4D02G283600.2
    ATTTGGGTAGCGCGGTGGATGGGTTAGGAGGAATGTGAGCGCGCCATGTGTTTGTCCGCCAGTGCCTAACTGCCGCACTGCCTCAAAAGGCGCGTGCTAGGGTACTGTACTTAATTAGCAGCGCCGTACGCGCTTGAGCTTGCATTGCATCTGCCATGGATATTGATCACGGCAGAGAGAGCGACGGCGAGATGGTGGGCACCATCGCCAGCTGCGGGCTGCTGCTCCACTCGCTTCTCGCCGGGCTCGGGCGTCGCGCCGCCGGGTTCGCCCGGAAGGTGGGCGGCGCCGCGCGGGAGGACCCGAGGCGGGTGGCGCACTCGCTCAAAGTCGGCCTGGCGCTCGCGCTGGTGTCCGTCGTCTACTTCGTCACGCCGCTCTTCAACGGCCTCGGGGTGTCCGCGATATGGGCCGTGCTCACCGTCGTCGTCGTCATGGAGTACACCGTCGGTGCCACGCTGAGTAAAGGCTTGAACAGAGCCTTGGCGACGTTGGTGGCTGGCTGCATCGCCGTCGGAGCTCATCAGTTAGCTGAATTAGCTGAACGCTGTGGTGATCAGGGAGAGCCCATAGTGCTCACCGTGCTCGTCTTCTTCGTAGCGTCAGCGGCGACGTTCTTGCGCTTCATCCCGGAGATCAAGGCCAAGTACGACTACGGCGTGACCATCTTCATACTGACCTTCGGTCTGGTGGCCGTGTCGAGCTACAGAGTGGAGGAGCTCATCCAGCTCGCGCACCAGCGGTTCTACACCATAGCCGTCGGCGTCTTCATCTGCCTCTGCACCACCGTCTTCCTCTTCCCCGTCTGGGCCGGAGAGGACGTCCACAAGCTCGCCTCCGGCAACCTCGACAAACTCGCTCAGTTCATTGAAGGAATGGAATTCAACTGCTTTGGCGAAAACAGTGTTGCAAATAATTTTGGGGGAAAGGATTCCCCCCAAATGCACAAGAGCGTCCTTAATTCGAAGGCCACTGAGGACTCTTTGTGCACCTTTGCCAAATGGGAGCCTCGTCATGGCCAGTTCAGATTTCGACACCCATGGAGCCAATACCAGAAGCTGGGAACTCTTTGTCGCCAATGTGCGTCTTCTATGGAGGCTCTTGCTTCATATGTCATCACAACCTCAAAAACCCAGTGCCCTGCTGCAGCCAACCCTGAGCTATCATGTAAGGTTCGAAAAACATGTGGCGAAATGAGCTTGCATTCCTCCAAGGTGCTTAGGGATCTCGCAATGGCAACTCGAACAATGACTGTGCCGTCTCCAGTGAATATCACCATGGCTACAGCCGTGAAAGCAGCGGAAAGCCTCAGAAGCGAGCTTGCAGAGAACACGGCTCTGTTGCAAGTGATGCATGTGGCCGTCACCGCAACACTTCTTGCGGACTTGGTTGATAGGGTGAAGGAAATCGCGGAATGTGTTGATGTCCTAGCAAGACTGGCGCACTTTAAGAACCCCGAGGACACAAAAAATGTCGTTGTCAGTACCGTGAGTCGAGGGATAGACGAACCTTTGCCTGACGTGGTTATTTTGTAAATCTTCAAAACATTGGTAGACTATATGGTGAAGAACATGGTAGTACTATAGTAGTACTATGTATCAATACTGGAGGGTCTTGTATTGGTTGATTTAGATTTATTACTGCTGAGACATGTTGGATATATGAGCGATTTTC
  • >TraesCS4D02G283600.1
    ATTTGGGTAGCGCGGTGGATGGGTTAGGAGGAATGTGAGCGCGCCATGTGTTTGTCCGCCAGTGCCTAACTGCCGCACTGCCTCAAAAGGCGCGTGCTAGGGTACTGTACTTAATTAGCAGCGCCGTACGCGCTTGAGCTTGCATTGCATCTGCCATGGATATTGATCACGGCAGAGAGAGCGACGGCGAGATGGTGGGCACCATCGCCAGCTGCGGGCTGCTGCTCCACTCGCTTCTCGCCGGGCTCGGGCGTCGCGCCGCCGGGTTCGCCCGGAAGGTGGGCGGCGCCGCGCGGGAGGACCCGAGGCGGGTGGCGCACTCGCTCAAAGTCGGCCTGGCGCTCGCGCTGGTGTCCGTCGTCTACTTCGTCACGCCGCTCTTCAACGGCCTCGGGGTGTCCGCGATATGGGCCGTGCTCACCGTCGTCGTCGTCATGGAGTACACCGTCGGTGCCACGCTGAGTAAAGGCTTGAACAGAGCCTTGGCGACGTTGGTGGCTGGCTGCATCGCCGTCGGAGCTCATCAGTTAGCTGAATTAGCTGAACGCTGTGGTGATCAGGGAGAGCCCATAGTGCTCACCGTGCTCGTCTTCTTCGTAGCGTCAGCGGCGACGTTCTTGCGCTTCATCCCGGAGATCAAGGCCAAGTACGACTACGGCGTGACCATCTTCATACTGACCTTCGGTCTGGTGGCCGTGTCGAGCTACAGAGTGGAGGAGCTCATCCAGCTCGCGCACCAGCGGTTCTACACCATAGCCGTCGGCGTCTTCATCTGCCTCTGCACCACCGTCTTCCTCTTCCCCGTCTGGGCCGGAGAGGACGTCCACAAGCTCGCCTCCGGCAACCTCGACAAACTCGCTCAGTTCATTGAAGGAATGGAATTCAACTGCTTTGGCGAAAACAGTGTTGCAAATAATTTTGGGGGAAAGGATTCCCCCCAAATGCACAAGAGCGTCCTTAATTCGAAGGCCACTGAGGACTCTTTGTGCACCTTTGCCAAATGGGAGCCTCGTCATGGCCAGTTCAGATTTCGACACCCATGGAGCCAATACCAGAAGCTGGGAACTCTTTGTCGCCAATGTGCGTCTTCTATGGAGGCTCTTGCTTCATATGTCATCACAACCTCAAAAACCCAGTGCCCTGCTGCAGCCAACCCTGAGCTATCATGTAAGGTTCGAAAAACATGTGGCGAAATGAGCTTGCATTCCTCCAAGGTGCTTAGGGATCTCGCAATGGCAACTCGAACAATGACTGTGCCGTCTCCAGTGAATATCACCATGGCTACAGCCGTGAAAGCAGCGGAAAGCCTCAGAAGCGAGCTTGCAGAGAACACGGCTCTGTTGCAAGTGATGCATGTGGCCGTCACCGCAACACTTCTTGCGGACTTGGTTGATAGGGTGAAGGAAATCGCGGAATGTGTTGATGTCCTAGCAAGACTGGCGCACTTTAAGAACCCCGAGGACACAAAAAATGTCGTTGTCAGTACCGTGAGTCGAGGGATAGACGAACCTTTGCCTGACGTGGTTATTTTGTAAATCTTCAAAACATTGGTAGACTATATGGTGAAGAACATGGTAGTACTATAGTAGTACTATGTATCAATACTGGAGGGTCTTGTATTGGTTGATTTAGATTTATTACTGCTGAGACATGTTGGATATATGAGCGATTTTC
CDS Sequence
  • >TraesCS4D02G283600.2
    ATGGATATTGATCACGGCAGAGAGAGCGACGGCGAGATGGTGGGCACCATCGCCAGCTGCGGGCTGCTGCTCCACTCGCTTCTCGCCGGGCTCGGGCGTCGCGCCGCCGGGTTCGCCCGGAAGGTGGGCGGCGCCGCGCGGGAGGACCCGAGGCGGGTGGCGCACTCGCTCAAAGTCGGCCTGGCGCTCGCGCTGGTGTCCGTCGTCTACTTCGTCACGCCGCTCTTCAACGGCCTCGGGGTGTCCGCGATATGGGCCGTGCTCACCGTCGTCGTCGTCATGGAGTACACCGTCGGTGCCACGCTGAGTAAAGGCTTGAACAGAGCCTTGGCGACGTTGGTGGCTGGCTGCATCGCCGTCGGAGCTCATCAGTTAGCTGAATTAGCTGAACGCTGTGGTGATCAGGGAGAGCCCATAGTGCTCACCGTGCTCGTCTTCTTCGTAGCGTCAGCGGCGACGTTCTTGCGCTTCATCCCGGAGATCAAGGCCAAGTACGACTACGGCGTGACCATCTTCATACTGACCTTCGGTCTGGTGGCCGTGTCGAGCTACAGAGTGGAGGAGCTCATCCAGCTCGCGCACCAGCGGTTCTACACCATAGCCGTCGGCGTCTTCATCTGCCTCTGCACCACCGTCTTCCTCTTCCCCGTCTGGGCCGGAGAGGACGTCCACAAGCTCGCCTCCGGCAACCTCGACAAACTCGCTCAGTTCATTGAAGGAATGGAATTCAACTGCTTTGGCGAAAACAGTGTTGCAAATAATTTTGGGGGAAAGGATTCCCCCCAAATGCACAAGAGCGTCCTTAATTCGAAGGCCACTGAGGACTCTTTGTGCACCTTTGCCAAATGGGAGCCTCGTCATGGCCAGTTCAGATTTCGACACCCATGGAGCCAATACCAGAAGCTGGGAACTCTTTGTCGCCAATGTGCGTCTTCTATGGAGGCTCTTGCTTCATATGTCATCACAACCTCAAAAACCCAGTGCCCTGCTGCAGCCAACCCTGAGCTATCATGTAAGGTTCGAAAAACATGTGGCGAAATGAGCTTGCATTCCTCCAAGGTGCTTAGGGATCTCGCAATGGCAACTCGAACAATGACTGTGCCGTCTCCAGTGAATATCACCATGGCTACAGCCGTGAAAGCAGCGGAAAGCCTCAGAAGCGAGCTTGCAGAGAACACGGCTCTGTTGCAAGTGATGCATGTGGCCGTCACCGCAACACTTCTTGCGGACTTGGTTGATAGGGTGAAGGAAATCGCGGAATGTGTTGATGTCCTAGCAAGACTGGCGCACTTTAAGAACCCCGAGGACACAAAAAATGTCGTTGTCAGTACCGTGAGTCGAGGGATAGACGAACCTTTGCCTGACGTGGTTATTTTGTAA
  • >TraesCS4D02G283600.1
    ATGGATATTGATCACGGCAGAGAGAGCGACGGCGAGATGGTGGGCACCATCGCCAGCTGCGGGCTGCTGCTCCACTCGCTTCTCGCCGGGCTCGGGCGTCGCGCCGCCGGGTTCGCCCGGAAGGTGGGCGGCGCCGCGCGGGAGGACCCGAGGCGGGTGGCGCACTCGCTCAAAGTCGGCCTGGCGCTCGCGCTGGTGTCCGTCGTCTACTTCGTCACGCCGCTCTTCAACGGCCTCGGGGTGTCCGCGATATGGGCCGTGCTCACCGTCGTCGTCGTCATGGAGTACACCGTCGGTGCCACGCTGAGTAAAGGCTTGAACAGAGCCTTGGCGACGTTGGTGGCTGGCTGCATCGCCGTCGGAGCTCATCAGTTAGCTGAATTAGCTGAACGCTGTGGTGATCAGGGAGAGCCCATAGTGCTCACCGTGCTCGTCTTCTTCGTAGCGTCAGCGGCGACGTTCTTGCGCTTCATCCCGGAGATCAAGGCCAAGTACGACTACGGCGTGACCATCTTCATACTGACCTTCGGTCTGGTGGCCGTGTCGAGCTACAGAGTGGAGGAGCTCATCCAGCTCGCGCACCAGCGGTTCTACACCATAGCCGTCGGCGTCTTCATCTGCCTCTGCACCACCGTCTTCCTCTTCCCCGTCTGGGCCGGAGAGGACGTCCACAAGCTCGCCTCCGGCAACCTCGACAAACTCGCTCAGTTCATTGAAGGAATGGAATTCAACTGCTTTGGCGAAAACAGTGTTGCAAATAATTTTGGGGGAAAGGATTCCCCCCAAATGCACAAGAGCGTCCTTAATTCGAAGGCCACTGAGGACTCTTTGTGCACCTTTGCCAAATGGGAGCCTCGTCATGGCCAGTTCAGATTTCGACACCCATGGAGCCAATACCAGAAGCTGGGAACTCTTTGTCGCCAATGTGCGTCTTCTATGGAGGCTCTTGCTTCATATGTCATCACAACCTCAAAAACCCAGTGCCCTGCTGCAGCCAACCCTGAGCTATCATGTAAGGTTCGAAAAACATGTGGCGAAATGAGCTTGCATTCCTCCAAGGTGCTTAGGGATCTCGCAATGGCAACTCGAACAATGACTGTGCCGTCTCCAGTGAATATCACCATGGCTACAGCCGTGAAAGCAGCGGAAAGCCTCAGAAGCGAGCTTGCAGAGAACACGGCTCTGTTGCAAGTGATGCATGTGGCCGTCACCGCAACACTTCTTGCGGACTTGGTTGATAGGGTGAAGGAAATCGCGGAATGTGTTGATGTCCTAGCAAGACTGGCGCACTTTAAGAACCCCGAGGACACAAAAAATGTCGTTGTCAGTACCGTGAGTCGAGGGATAGACGAACCTTTGCCTGACGTGGTTATTTTGTAA
Protein Sequence
  • >TraesCS4D02G283600.2
    MDIDHGRESDGEMVGTIASCGLLLHSLLAGLGRRAAGFARKVGGAAREDPRRVAHSLKVGLALALVSVVYFVTPLFNGLGVSAIWAVLTVVVVMEYTVGATLSKGLNRALATLVAGCIAVGAHQLAELAERCGDQGEPIVLTVLVFFVASAATFLRFIPEIKAKYDYGVTIFILTFGLVAVSSYRVEELIQLAHQRFYTIAVGVFICLCTTVFLFPVWAGEDVHKLASGNLDKLAQFIEGMEFNCFGENSVANNFGGKDSPQMHKSVLNSKATEDSLCTFAKWEPRHGQFRFRHPWSQYQKLGTLCRQCASSMEALASYVITTSKTQCPAAANPELSCKVRKTCGEMSLHSSKVLRDLAMATRTMTVPSPVNITMATAVKAAESLRSELAENTALLQVMHVAVTATLLADLVDRVKEIAECVDVLARLAHFKNPEDTKNVVVSTVSRGIDEPLPDVVIL
  • >TraesCS4D02G283600.1
    MDIDHGRESDGEMVGTIASCGLLLHSLLAGLGRRAAGFARKVGGAAREDPRRVAHSLKVGLALALVSVVYFVTPLFNGLGVSAIWAVLTVVVVMEYTVGATLSKGLNRALATLVAGCIAVGAHQLAELAERCGDQGEPIVLTVLVFFVASAATFLRFIPEIKAKYDYGVTIFILTFGLVAVSSYRVEELIQLAHQRFYTIAVGVFICLCTTVFLFPVWAGEDVHKLASGNLDKLAQFIEGMEFNCFGENSVANNFGGKDSPQMHKSVLNSKATEDSLCTFAKWEPRHGQFRFRHPWSQYQKLGTLCRQCASSMEALASYVITTSKTQCPAAANPELSCKVRKTCGEMSLHSSKVLRDLAMATRTMTVPSPVNITMATAVKAAESLRSELAENTALLQVMHVAVTATLLADLVDRVKEIAECVDVLARLAHFKNPEDTKNVVVSTVSRGIDEPLPDVVIL