Information report for 678347781
Gene Details
Functional Annotation
- GO:0006511 — Biological Process — ubiquitin-dependent protein catabolic process
- GO:0016579 — Biological Process — protein deubiquitination
- GO:0000166 — Molecular Function — nucleotide binding
- GO:0003676 — Molecular Function — nucleic acid binding
- GO:0036459 — Molecular Function — thiol-dependent ubiquitinyl hydrolase activity
Family Introduction
- Understanding the role of transcription factors (TFs) is essential in reconstructing developmental regulatory networks. The plant-specific GeBP TF family of Arabidopsis thaliana (Arabidopsis) comprises 21 members, all of unknown function. A subset of four members, the founding member GeBP and GeBP-like proteins (GPL) 1, 2, and 3, shares a conserved C-terminal domain.
- Here we report that GeBP/GPL genes represent a newly defined class of leucine-zipper (Leu-zipper) TFs and that they play a redundant role in cytokinin hormone pathway regulation. Specifically, we demonstrate using yeast, in vitro, and split-yellow fluorescent protein in planta assays that GeBP/GPL proteins form homo- and heterodimers through a noncanonical Leu-zipper motif located in the C-terminal domain.
- A triple loss-of-function mutant of the three most closely related genes gebp gpl1 gpl2 shows a reduced sensitivity to exogenous cytokinins in a subset of cytokinin responses such as senescence and growth, whereas root inhibition is not affected. We find that transcript levels of type-A cytokinin response genes, which are involved in the negative feedback regulation of cytokinin signaling, are higher in the triple mutant. Using a GPL version that acts as a constitutive transcriptional activator, we show that the regulation of Arabidopsis response regulators (ARRs) is mediated by at least one additional, as yet unknown, repressor acting genetically downstream in the GeBP/GPL pathway.
- Our results indicate that GeBP/GPL genes encode a new class of unconventional Leu-zipper TF proteins and suggest that their role in the cytokinin pathway is to antagonize the negative feedback regulation on ARR genes to trigger the cytokinin response.
Literature and News
Sequences
CDS Sequence:
- >678347781|Utricularia_gibba|GeBP|678347781
ATGGCGGAAGGTGAGAGGGTCAAGCTCTTCGTGGGTCAAGTTCCTAAGCAAATGACGGAATCGCAGTTGTTGCGAATGTTCCAGGAGTCTATAGTCGCCAACGAAGTCAACATAATCAAGGATAGAGTGACGCGTGCTTCACGAGGCTGTTGTTTTCTGTTTTGTCCGTCTAGAGAGGAAGCTGAGAAGGCAATAAATATCTTTCATGATAAGAAAACGCTGCCTGGGGTTGAGCACAAACTTTTCATTGGAATGCTTCCCAAAAATGTTTCTGATGATGAGGTTTCTGCTCTGTTTTCTAAATATGGGATAATAAAGGAGTTGCAATTTTTAAGAGGCTCTCAGCAAACTAGCAAAGGTTGTGCTTTCCTAAAATTTGAAACAAAGGAAGAGGCTGTTGCAGCAATTGAAGCACTTAACGGTATCCATAAGATTGAGGGCTCTACGGTTCCCTTGGTGGTCAAATGGGCTGATACAGAAAAAGAAAGATTGGCTCGGAAGGCACAAAAAGCCCTCTCGCAGGCCTCCAGTCTTCACACTACTGGAATCAGACAGCGTCCATCTTTGGTTGGTGCACTCCCCATGAGTCATATGCCTTCATATAATGGATGTAGTTACCAGGGGGGCACAATGCGAGGTCTAAGACCTGGTCTTTCGCCTGGAATGAGCCCAGGAAAGTATGCTGTGCCACCTGCAAATTATGTTGGATCTAGTTATCCAGCTTTGCCTGGTGTTCAATACCCATTCTCCTATTCTGGAGGAATCATGAGCAATCGGTCAGGAATGTCTGTAAATTCCTTGTCACCTCACGCAGCAAATAGCCAGTCTGCATCACCTTCCGGTGTTGGATCAAGTTCCGGGGGCCAAATGGAAGGCCCCCCTGGAGCCAACTTGTTTATTTACCATATCCCACAGGAGTTTGGGGATGAGGAGCTCGCCAGCGCATTTCAGCGTTTTGGCAGGGTACTAAGTGCGAAAGTCTTTGTTGACAAAGCAACTGGTGCAAGCAAATGCTTCGGATTTGTGAGTTATGATTCACCAGCAGCAGCACAGAGCGCCATCAGCACAATGAATGGGTATCAGTTAGGTGGCAAAAAACTGAAGATTCCAGAGCAACCTTGCCAGGTCATTGTAATGGTAACCTGTCCAGTGGCATTTAGTGATGAGTTGCAAGTAGATGTTTCATACTCCATGAAGCTTGTTTTGAACGTACAGACTCACCAGGCAATACGAAGCCCACACATAGATCGTCTGGTCAATCCCACATCCATGTCTCGGTGCCGTCCTGCAGAAAAACATCATCAAGAGAGACCAAACAGAATGCCGAGGGAGGAGTTGAAGGAGATCGACAAGGTATTGCTTTTTGGGTCATTCACCGAGAATGAAACGCGTTCCTGGATAAGTGGAAAGAAGGAGAATCCCTGTGAATCTCGGGAAAAAGGTGCAGTAAAAATCAGTTCTTCGGAAGCTGTTCAACCGCAGGCGATTGATGGGTCTGTGGGTGGGCGTGCTTTCTCCGTCCAAAAGGGTAAAACTGAACCACAAAGGAATTCAGCAACCAGTGCCTTTTCCAATGCGCACGAAAATGGAAATACTTCCATTTCCAAGTCCCATGTTGAAAAAAAGGTCATATTTGGTGGAGCAGGCCTGAGTAGAAGAGCATCAGACGAGAACTTTGTCTCAAATCTCCAAAATTCTGATAACATAAAGCTGAATGGATCCAATGCTGCTTCTCCTGGTGATGTGCTTCAAAACTCTAAGAGAGTAACTGGTTTTCATGGTCAAGATGTACTTCCTTGTGGATTGATCAACCCGGGAAATTTGTGCTTCCTCAATGCTACACTTCAAGCTCTTTTATCTTGCTCTCCATTCGTTCGGCTGTTGCAAGAATTGAGAAGTCGGAACCTTTCCAAGGCTGCCTACCCAACAATATCCGCATTTGTTGATTTCATTAATGAGGTTAGTGTTGTTGGTGGAATCAATGTTGCGGATAAAGATCCTGCTGTAATTGAAACACGAAAGTCATATACACCTGTGATGTTTGAAACTGTCTTGAGGAAATTTTCTCCTGACATATCAGGCAACTTTTTAGGCAGAACTAGACAAGAGGATGCACAAGAGTTTCTGAGTTTCATGATGGATCAAATGCATGATGAGCTGCTGAAGCTTGATGGGCTGACTGTCTCAAAATCAACTGTCGTCTCTACTGCTGAAGATGATGGGTGGGAAACCATTGGTCCTAAGAATAAATCTGCTGTTACAACAACCCAGAGTCTCATTCCTTCTGAACTCAGCTCCATATTCGGTGGACAATTGCGAAGTGTTGTAAAAGCAAAAGGAAACAAAGCTTCAGCCACTGTTCAACCGTTTCTGATGCTTCACCTCGACATTTCTCATGAATCTATTCACACTGTTGAAGATGCTCTACATTTCTTTTCCGCCCCAGAGACATTGAACGAGTATCGAACACCAACATCACAAAGGGCGGCACCTGTTTCAGCCCGGAAGTCAATCAACATTCAGTCACTTCCAAAGATACTCATTTTGCATCTGAAGCGATTCGGCTATGGCGCCCACGGAAGCACTAAGCTGCAAAAGGCTGTTCGTTTCCCCCTTGAACTAGTTTTACACCGTGATCTGCTCGTGTCCGCCGCCGTGGAGGGCCGTAAGTATGAGCTCGTGGCAACCATAACTCACCATGGAAACATAGCGTCAAAAGGACACTACACAGCTGATGCTCGAAGAAACGACGGCCTCCTGTGGCTCCGGTTCGACGATGCCTCCGTGGAGGCCATTCCAAGAAGCAGAGTGTTGCATGATCACGCTTATCTTCTGTTTTATAGACAGAGATGGGTTGGTGGGGGTATAAACCTGGGACTGGCGTTCAGGTTCAGGCCGGTAGGGTTTGGATTGGGTCTGGTGATCGGACCCGGCGGTGCGGATCACGAAACACGACGGTTCATTTCCCCCGAAATATTGAAGCCTCGCCGGGTCGCTGCCATGGCCTCTGTCGCTGCCGTTGCTGGTCACGGCGTCGTCTTCAACGACGACGAGGATCTTCTCGATGATGACGACGACCAGATCTCTGATCACGATGAAGATGATTCCGCGTCCTCGGCGCCTCTCGCGCTTGATGGCGCTCCTTCCACTGCCCTCGTGACTGTGGCAGTCCACGGCGTCCCTGATCTCCACCATAATCAACAGCAACAGGACCTTTTTCTGCCGGAGTCGAGAAAGCCTGACGAGTCCCGGAAGCTCTTCCAGCGTCTGTGGACGGACCAGGACGAGATTGAGCTGTTGCAAGGGTTCCTGGATTACACCACTCAGCGTTGCGGTCAGTACGGTTCGTCGCAGCACCACCATGATACGACGGCGTTTTATGACCAGATCAGGAGCAAGTTCCAGCTCGATTTCAACAAGAGTCAGCTTGTTGAGAAGCTTCGGAGGCTGAAGAAGAAGTATCGTAATCTAGTCAACAGGTTCAGCTCCGGTAAAGATTATATCTTTAAGAGCCCCCACGATCAGTTGACCTTCGAAATTTCTTCCAAAATTTGGGGCAACGCTAACGGCTCTGCTGCTTACCGGTCCACCGGCGTTGAGGAAGACGACGATGATCTCAATAACCCAGCTTTTATTAGCTGTCAGAACCCGATCCCAAGTAGGATCGATGTGAACAGCAAAGCCCCCCGACCGCGAAAGAGGAATCGTGCTGGGCCTGTGAAATTGGAGGAGAAAATGCCGTCGCACACAAACTACTGTAACGTCAACAATATTCAGCCGCCCACGGTGGGTATAACACCCATTGCCGCTCCCGTCCTGGGCATCGGGATTTCCGGGCCTATCTCCACCGTGATCGAAGAGACAATTAAGAGCTCTTTGTCTCCTGTACTCAAGGAATTGCTGGGAAATTCGTCTGTTCCTAGTGGCTTATTCTGTGGACATGGTTCAGGAACCACTAGTCCCCTGTCGTTGTGTTTCCCTGGAGCTGTCGCCGGCGATAAAACGTCCGATGATCGGTGGAGGAAACAACAGATTCTTGAGTTAGAAGTGTTCTCTAAGAGGCTTGAGCTAGTGCAAAGCCAGATAAGGGATCAGTTGCAGGAGCTTAGATTCATGGAGAACTGA
Protein Sequence:
- >678347781|Utricularia_gibba|GeBP|678347781
MAEGERVKLFVGQVPKQMTESQLLRMFQESIVANEVNIIKDRVTRASRGCCFLFCPSREEAEKAINIFHDKKTLPGVEHKLFIGMLPKNVSDDEVSALFSKYGIIKELQFLRGSQQTSKGCAFLKFETKEEAVAAIEALNGIHKIEGSTVPLVVKWADTEKERLARKAQKALSQASSLHTTGIRQRPSLVGALPMSHMPSYNGCSYQGGTMRGLRPGLSPGMSPGKYAVPPANYVGSSYPALPGVQYPFSYSGGIMSNRSGMSVNSLSPHAANSQSASPSGVGSSSGGQMEGPPGANLFIYHIPQEFGDEELASAFQRFGRVLSAKVFVDKATGASKCFGFVSYDSPAAAQSAISTMNGYQLGGKKLKIPEQPCQVIVMVTCPVAFSDELQVDVSYSMKLVLNVQTHQAIRSPHIDRLVNPTSMSRCRPAEKHHQERPNRMPREELKEIDKVLLFGSFTENETRSWISGKKENPCESREKGAVKISSSEAVQPQAIDGSVGGRAFSVQKGKTEPQRNSATSAFSNAHENGNTSISKSHVEKKVIFGGAGLSRRASDENFVSNLQNSDNIKLNGSNAASPGDVLQNSKRVTGFHGQDVLPCGLINPGNLCFLNATLQALLSCSPFVRLLQELRSRNLSKAAYPTISAFVDFINEVSVVGGINVADKDPAVIETRKSYTPVMFETVLRKFSPDISGNFLGRTRQEDAQEFLSFMMDQMHDELLKLDGLTVSKSTVVSTAEDDGWETIGPKNKSAVTTTQSLIPSELSSIFGGQLRSVVKAKGNKASATVQPFLMLHLDISHESIHTVEDALHFFSAPETLNEYRTPTSQRAAPVSARKSINIQSLPKILILHLKRFGYGAHGSTKLQKAVRFPLELVLHRDLLVSAAVEGRKYELVATITHHGNIASKGHYTADARRNDGLLWLRFDDASVEAIPRSRVLHDHAYLLFYRQRWVGGGINLGLAFRFRPVGFGLGLVIGPGGADHETRRFISPEILKPRRVAAMASVAAVAGHGVVFNDDEDLLDDDDDQISDHDEDDSASSAPLALDGAPSTALVTVAVHGVPDLHHNQQQQDLFLPESRKPDESRKLFQRLWTDQDEIELLQGFLDYTTQRCGQYGSSQHHHDTTAFYDQIRSKFQLDFNKSQLVEKLRRLKKKYRNLVNRFSSGKDYIFKSPHDQLTFEISSKIWGNANGSAAYRSTGVEEDDDDLNNPAFISCQNPIPSRIDVNSKAPRPRKRNRAGPVKLEEKMPSHTNYCNVNNIQPPTVGITPIAAPVLGIGISGPISTVIEETIKSSLSPVLKELLGNSSVPSGLFCGHGSGTTSPLSLCFPGAVAGDKTSDDRWRKQQILELEVFSKRLELVQSQIRDQLQELRFMEN