Information report for XP_016481211.1
Gene Details
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Functional Annotation
- Refseq: XP_016481211.1 — PREDICTED: protein FAR1-RELATED SEQUENCE 5-like isoform X1
- Swissprot: Q9SZL8 — FRS5_ARATH; Protein FAR1-RELATED SEQUENCE 5
- TrEMBL: A0A1S4AX63 — A0A1S4AX63_TOBAC; protein FAR1-RELATED SEQUENCE 5-like isoform X1
- STRING: XP_009631560.1 — (Nicotiana tomentosiformis)
- GO:0008270 — Molecular Function — zinc ion binding
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Actinidia chinensis: Achn264411
- Cicer arietinum: XP_012574577.1
- Glycine max: Glyma.11G088700.1.p
- Lotus japonicus: Lj2g3v1573090.2, Lj2g3v1573090.1
- Malus domestica: MDP0000261311
- Manihot esculenta: Manes.02G117400.1.p, Manes.15G114300.2.p
- Populus trichocarpa: Potri.011G145800.1
- Prunus mume: XP_008242120.2
- Prunus persica: Prupe.7G188000.1.p
- Ziziphus jujuba: XP_015889514.1, XP_015889513.1, XP_015889512.1
Sequences
CDS Sequence:
- >XP_016481211.1|Nicotiana_tabacum|FAR1|XP_016481211.1
ATGGAGTTTGAACCGTTGGGAATCAGGAATGATGTGATAGAATTTGATATGTTTGGCTTAGGAGTAGGAGACGAAACCATTGACATTGAACACCCTATTGAAGAAGGGGAAAATAATCAATTAATGGACCCTTTTGATGGTATTGTTAGTGGTAGAATTTCTGGTGAAGTTTATGGGGATATGAATCTTGAATCTTATGAAGGAATGGAGTTTGAGTCGGAAGAGGCTGCGAAAGCCTTCTATAATTTGTATGCACGTAGGGTAGGGTTTAGTACGCGTATTAGCATGTCTAGGAGGTCTAGGCGTGACGGGGCTATTATTCAGAGGTCGTTCGTTTGTGCAAGAGAGGGGTTTCGTGTTGAGAAGGAGAAGCCTGGGCATGATGGCGGTGGTGCTAGGATTAAGAGACCCCGAGCTGAGACTAGGGTCGGGTGTAAGGCGATGTTAGTTGTTAAGATTCAGAATTCGGGGAGATGGGTTGTGTCGGGATTCGTAAAAGAACATAACCATGAGCTTGTTCCACCTGATAAGGTGCATTGCCTGCGTTCACACCGACATGTGTCGGGACCAGCTAAGTTGCTGATTGATACATTACAGGGAGCTGGAATTGGTCCGAGTGGGATAATGTCTGCCTTGATCAAGGAGTATGGTGGGATTAGTAGTGTTGGATTTACGGAACGTGATTGTAGAAATTACATGAGAAGTAGTAAACAGAGAACTCTTGGGGGTGATCCCCAGACTCTCCTTGATTATTTGAAACAAAAGCATTTGGAAAATCCATCGTTTTTCTATGCTGTGCAAGGAGATGAGGATCATTGTATGAGTAATATCTTTTGGGCTGACCCTGAGGCTCGGAGTAATTTTATTTATTTCGGTGATACTGTTACTTTTGACACCACTTACAGGTCAAACAGGTATAGGTTACCATTTGCTCCATTCACAGGGGTGAAGCATCATGGACAACCAGTGCTATTTGGTTGTGCTCTCTTATTAAATGAATCTGAGGCATCTTTTAGTTGGTTGTTTAGAACTTGGCTGATGGCAATGTGTGGGAAGCCGCCACTCTCAATCACAACTGACCATGATCGAGTGATTCGTTTGGCTGTTAGACAGGTTTTCCCCGAAGCACGTCACAGGTTCTGCAAATGGCATATTTTCAAAGAATGCCAAGAGAAGTTGTCCCACTTGCTCTCCGAGCATATTAATTTTGAAGCAGAGTTTCATAAATGTGTTAGCTTTACGGAGTCCATTGAAGAGTTTGAAGCTTGTTGGTTTACCCTGATTGATAAGTATGATCTTGGGGAAAATGAGTGGCTTCAAGCCATTTACGTTGATCGCAAGCAATGGGTTCCGGTCTATTTGAGGGATACATTTTTTGCCGAAATATCTGTAATGCAGCGTAGTGACAGTATGAATTCATATTTTGATGGGTATGTGAATGCATCAACTACTCTTCAACAATTCATTAAGCTGTATGAAAAGGCTGTAGAGAGTCGCTACGAGAAGGAAGTTAAAGCAGATTACGACACAATGCATATTGGCCCCGCTCTGAAGACCCCGTCACCCATGGAGAAGCAAGCTGCTGAGATTTATACTAGGAAACTGTTCATAAAGTTTCAAGAGGAGCTCGTTGAAACACTCACATTCTTGGCAACTAAAGTTGATGATAACGAAGCAACAACTGTATTTCAGGTGGCTAAATTTGGGGAGAGTCATAAGTCTTACTTAGTTCACTACAATGTCAGGGAAATTAAAGCATCGTGTAGCTGTCAGATGTTTGAATTCTCCGGTCTCCTTTGCCGGCATATACTAACAGTCTTCAGAGTCACCAATGTTCTCACGCTTCCTCCATGTTATGTTTTAAAGCATTGGACTAGGAATGCTAAAAGTGGTCTCATGTTGGAAGAACAGACGTGTAATTTGTTAAATAATTGTCAGGAATCTTTTAATGTCCGATATAAAAATCTTCAGCTAGAAGCCCTCAAATATGTAGATGAAGGTGTGAAAGATGAGGAATGTTACCAGGTGGCTATGGATGCCTTGCGAGAAGCTGCACATAAAGTTGCTATTGCTAAGAAAACTGATGGGGGAGTTTCTAATTCTAGTGGAACTTGCAAAGGAGAGTCTGCCACTCAGGAGAGCCATGTAAATAATCCCTTCGAGGCTCGTCAATTGGGTAGGTGTCCTTCAACACCCGTGGATGAGCAGGACAAGAAAATACAGAAACTTACACGGAAGCTGGAACGTGCAAGACAAAAATGTGAATTATATCGGACTAACTTATTGACAGTCTTGAAAGATATTGAGCAACTGAAGCTTCAGTTAACAGTTAAAGTTCAGAGTATTAAGTTAGGCATGAAAAATTAA
Protein Sequence:
- >XP_016481211.1|Nicotiana_tabacum|FAR1|XP_016481211.1
MEFEPLGIRNDVIEFDMFGLGVGDETIDIEHPIEEGENNQLMDPFDGIVSGRISGEVYGDMNLESYEGMEFESEEAAKAFYNLYARRVGFSTRISMSRRSRRDGAIIQRSFVCAREGFRVEKEKPGHDGGGARIKRPRAETRVGCKAMLVVKIQNSGRWVVSGFVKEHNHELVPPDKVHCLRSHRHVSGPAKLLIDTLQGAGIGPSGIMSALIKEYGGISSVGFTERDCRNYMRSSKQRTLGGDPQTLLDYLKQKHLENPSFFYAVQGDEDHCMSNIFWADPEARSNFIYFGDTVTFDTTYRSNRYRLPFAPFTGVKHHGQPVLFGCALLLNESEASFSWLFRTWLMAMCGKPPLSITTDHDRVIRLAVRQVFPEARHRFCKWHIFKECQEKLSHLLSEHINFEAEFHKCVSFTESIEEFEACWFTLIDKYDLGENEWLQAIYVDRKQWVPVYLRDTFFAEISVMQRSDSMNSYFDGYVNASTTLQQFIKLYEKAVESRYEKEVKADYDTMHIGPALKTPSPMEKQAAEIYTRKLFIKFQEELVETLTFLATKVDDNEATTVFQVAKFGESHKSYLVHYNVREIKASCSCQMFEFSGLLCRHILTVFRVTNVLTLPPCYVLKHWTRNAKSGLMLEEQTCNLLNNCQESFNVRYKNLQLEALKYVDEGVKDEECYQVAMDALREAAHKVAIAKKTDGGVSNSSGTCKGESATQESHVNNPFEARQLGRCPSTPVDEQDKKIQKLTRKLERARQKCELYRTNLLTVLKDIEQLKLQLTVKVQSIKLGMKN