Information report for XP_009764132.1
Gene Details
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Functional Annotation
- Refseq: XP_009764132.1 — PREDICTED: uncharacterized protein LOC104215892
- TrEMBL: A0A1U7V7M2 — A0A1U7V7M2_NICSY; uncharacterized protein LOC104215892
- STRING: XP_009764132.1 — (Nicotiana sylvestris)
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Actinidia chinensis: Achn337331
- Capsicum annuum: CA06g21800
- Juglans regia: WALNUT_00013464-RA
- Nicotiana benthamiana: Niben101Scf00217g02009.1
- Nicotiana tabacum: XP_016513926.1, XP_016478794.1, XP_016478795.1, XP_016478796.1
- Petunia inflata: Peinf101Scf02372g00007.1
- Populus trichocarpa: Potri.007G129500.1
- Prunus mume: XP_008222480.1
- Prunus persica: Prupe.1G196400.1.p
- Salvia miltiorrhiza: SMil_00012162-RA_Salv
- Sesamum indicum: XP_011098324.1
- Solanum tuberosum: PGSC0003DMP400046912
- Vitis vinifera: GSVIVT01010788001
- Ziziphus jujuba: XP_015886992.1, XP_015886991.1
Sequences
CDS Sequence:
- >XP_009764132.1|Nicotiana_sylvestris|FAR1|XP_009764132.1
ATGGATGTTGTCATCAGGCAAATCATTCACGAAATTAAGCAAGATATCATAGAAGCATTCGAGGTGAAACTGGATGATACTCTATTAACTACTTCCCCCGTGTGCGGAACTGCAGAAGCGTGTGGTCAGCCGCAAATGCGGCGAGATGAGGGAAAGTCAGAAGTCGTAGGTGCAGGTGCTTTATCGCACATACGGGACCGCATGGGCGGTTGGTTAGACCGCAGAAGCGAGGAAATGGCCGCAGGTGCGGAAACCCTGGGCCAGAACCTATATAAGAGTTGTATCTATATTCAAACTGCAAATCATAATCTTTACTCTAATGTGGATATCCTTCAGTGTGGAGTATTTACACTCTGTTTTAAGTTTTTCTATGTCTATAAACAAATTACTGACGCAATTGATGCAACAGTGGACTTTGTGGTTGATGATAACATAATAGGCGAGGTGGAAGAGGGCACGGTGGTGGAAGTCAGGGCAGTAGATGAAAATGCAGTTGGAGGAAGTTCCAGTGAGGAGGCACTTCACGACGTGCATGATGTCAAGGTAGATGAAGACCTTTCTGATAGGGAGGTCGTTCCATTAGAAGAATCACAATCACTAGGTAATTCAACTTCAGATGAACCTTATGTTGGCCAGGAGTTTGAATCTGAAGCAGCAGCACATGCATTTTATAATACATATGCAACAAGAGTGGGGTTCATAATCCGGGTTAGCAAGCTTTCGCGATCTAGGCGCGATGGCTCTGCTATTGGTCGAGCACTAGTTTGCAACAAGGAGGGCTTTAGAATGCCTGATAAGCGGGAAAAGATAGTGCGACAAAGAGCAGAGACTAGGGTTGGTTGCAGAGCGATGATATTAGTTAGAAAAGTAAACTCTGGGAAGTGGGTTGTCACAAAATTTGTCAAGGAGCACACTCATCCATTAAGCCCAGGCAAAGGTCGTAGGGACTTGATCTATGATCAATATCCGAATGAGCATGACAAAATCCGGGAGCTATCACAACAACTGGCAGCTGAGAAAAAGAGATCAGCAACATATAAGAGGCATTTGGAAATGATATTCGAGCACATTGAGGAGCACAACCAATCTCTGTCCAAGAAGATCCAAGATATTGTACACAATGTAAGGGAGATGGAGTCTAGAGATCAACACCATCATAGATAG
Protein Sequence:
- >XP_009764132.1|Nicotiana_sylvestris|FAR1|XP_009764132.1
MDVVIRQIIHEIKQDIIEAFEVKLDDTLLTTSPVCGTAEACGQPQMRRDEGKSEVVGAGALSHIRDRMGGWLDRRSEEMAAGAETLGQNLYKSCIYIQTANHNLYSNVDILQCGVFTLCFKFFYVYKQITDAIDATVDFVVDDNIIGEVEEGTVVEVRAVDENAVGGSSSEEALHDVHDVKVDEDLSDREVVPLEESQSLGNSTSDEPYVGQEFESEAAAHAFYNTYATRVGFIIRVSKLSRSRRDGSAIGRALVCNKEGFRMPDKREKIVRQRAETRVGCRAMILVRKVNSGKWVVTKFVKEHTHPLSPGKGRRDLIYDQYPNEHDKIRELSQQLAAEKKRSATYKRHLEMIFEHIEEHNQSLSKKIQDIVHNVREMESRDQHHHR