Information report for TRIUR3_33706-P1
Gene Details
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Functional Annotation
- Refseq: XP_020196649.1 — vacuolar protein-sorting-associated protein 11 homolog
- Swissprot: Q9SJ40 — VPS11_ARATH; Vacuolar protein-sorting-associated protein 11 homolog
- TrEMBL: M8A192 — M8A192_TRIUA; Vacuolar protein sorting-associated protein 11-like protein
- STRING: TRIUR3_33706-P1 — (Triticum urartu)
- GO:0006886 — Biological Process — intracellular protein transport
- GO:0016192 — Biological Process — vesicle-mediated transport
- GO:0009705 — Cellular Component — plant-type vacuole membrane
- GO:0043234 — Cellular Component — protein complex
- GO:0005515 — Molecular Function — protein binding
- GO:0008270 — Molecular Function — zinc ion binding
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Actinidia chinensis: Achn360501
- Brassica rapa: XP_009116658.1, XP_009116657.1
- Fragaria vesca: mrna08567.1-v1.0-hybrid
- Malus domestica: MDP0000214642
- Panicum virgatum: Pavir.2NG591000.1.p, Pavir.6NG183000.1.p, Pavir.8KG166300.1.p, Pavir.6NG101100.1.p, Pavir.9KG490500.1.p
- Prunus mume: XP_016652765.1
- Pyrus bretschneideri: Pbr006864.2, Pbr002461.2
Sequences
CDS Sequence:
- >TRIUR3_33706-P1|Triticum_urartu|FAR1|TRIUR3_33706-P1
ATGGCTTCTCCGAGTCTTGCATCCCTGACGACTTCTCCGAGGAGTGTTCCATGGAACCAGGACACTTACGAACAATCTCATGCACCAAAACAACGGACATTTACATGCTCTCGTCAAGGCCATCGCCGGGAAGATAAAAGAGAGGAGACATTCACTTACAGTAGGCCTGAAACACGATGTGGTTGTGATGCCCGTATGAAGATTAGCCTTAGAGATGGATTTTATTATGTGTATGAATTCGAGGCGTCGCATAACCACACTCTTGCTAATGGACTCATGGCCCAATATTTGAGATCCCAGAGAAAAGTGACAGAAGCACAGATAGCAAACATAGAGGTCACAAAGTCAGTTGGCATTTCAAATAAAGCAACAATCGATTTGATGGCTAAAGAAGCAGGTGGAAGTGAAAACCTTGGCTTTACACCTCAAGATATGAAAAATTGTTTGTACTCAAAGAGAACAATAAAAGCAAAAGTAGGTGACACAGGGGGAGTATTAGAATACATGGAGAAAAAGGTCTCAGAAGATGTCAACTTCTTCTATTCAATTCAAGTGGATGAGGATGAGTTGATAACTAATATTTTTTGGGCCGACTCCAAAATGGTTTCAGACTATGCAATGTTCGGCGATGTTATATGCTTTGACACCACATACAGAAAACTAGATGATGGACGTCCATTTGGTTTGATTGTCGGGGTGAATAATCATAAGAAAACTATTGTCTTTGGTGCTGCACTTCTCTATGATGAAACCGCTGCTAGTTTTGCTTGGCTTTTCAGAACTTTCCTAAAAGTTATGTCAGGAAAACATCCACGTACAATTCTCACCGATGAAGATGCGGCAATGGCGAAGGCAATTCGTGAAGTCTTCCCTCATTCTCACCATAGACTTTGTGTGTGGCATATGAATCAAAATACTTGCAAGCACCTCGCCGGAGTTGTTGACGAATATAAGAAGTTCAATGTTGATTTTCAGCACTGTATATATGATATAGAAGAGGAGGATGAATTTGTAGGTGCATGGGATAAAATGATTGACAAGTATGGACTATGTGACAATGAATGGCTGCAAAGGCTATTTGAGAAAAAGGAACATTGGGCATTAGTGTACGGTAAAAATATGTTCTCTGCCCGCATGAGCACTACCCAAAGAAGTGAAAGCATGAACAATGAACTGAAGAGATACATTAGTATTAAATATGACATGCTTACTTTCTTTGAGCATTTTGAACGGCTAGTTTCGGATAAAAGATGTGAAGAGGTGAAGTATGACTTTAAAGCAACACAGACTACTCCCAAGTTGAAGGCTGAATCTAGTTATATGTTAAAACAAGCTGCAGCTACATATACTCCAGCAATATTCAAGATGGTTCAAGATCAAGTGCTTCGAACTCTGAACTATGACATGATCCTTTGTGATGAAAGTGACACAGAGGTGAAGATTTATGTAGTAAAATTTCATGGCACACAACGTGAACATGTTGTTAGATTCATTCCAAAAGAAGAAAAGGTTAGCTGCAGTTGCAAAAGGTTTGAGTTTGCTGGAATTCTTTGCTCTCATTGCTTAAAGGTACTTGATATTAACAATATCAAGCATATCCCTGGGGAATATATCTTGAAAAGATGGACAATTGATGCCAAAGTTCTAGATATAACAAGCAAATGCAACCCACATGATAACCTAAAAGCAAGAATGTCTAACTGCTACAAGGAACTATGCATAATATTTGTGAAAATAGCTGCTCGTGCCGCTGAGTCAGAAGAATCATACTATAAGGCGCAATTAGCACAAGATGTGGAGAAATGCTTGAAAATTAGAGCTGACCCAGATTTGGTTAGCGTCGATCCACATTTGGTTCATGTTGATCCAAATTTGGTTCACGGTGATCCAGATTTGGTTAGCGCTAATCAAGATTTGGTCAGTGCTGATCCAGATTTGGGTAACTCTATTACTAAAGAAGGAACGAACATCGCCTCTAGTAGAATTTCAAAACACGGTGAGGGACTTGCAAAACCAAAAGGTATGAAGGTTAAGGAGAAGACTGTGAAAGGATCAAAAAGACCTATTGGTGGATTTGAGAAAGCAACTTCAAAGAAGAAAAAGAACACAAATGATATTGTGCATCTAGAGCCTCGTGGCCCCTCAACGGGTCCTTTGAAGAAAGCAACTTTAAAGAAAAAAAAGAACACAAATGATATTATACAACTACAGCCTCATGACCTGCCGACGGGTCATCCGACAAATAACATGTACACCTCGCTGCAGGGTACCATTGGGGCCTCTAACTCTGGTTTGATGACAGGAGAAAATCAAGACTTCCTCCATCAACCGAATCCAGCATCTATCCTAAATGCTTCAATGCTGCAATCAAGTGGTCATACCATCCCAATGCCACCGCACATCCAAGAATACGGCAATACTCCAGTGCAAAGAAATGTTCTTGTCACGGTTGGAGATGATGATCAAGCATCTTCACAGTCATCGGCAGTCTGTCTAAAAGTTTTCGACCTTGATAAAGTACAAGAAGAGGGTTCAAGTACGACAACCCCTTTCTGTGTTCAAATTTTGCGGGTCTTCACGAATCAATTTCCTGAGGCCAAGATTACATCATTTTTGGTTTTAGAAGAAGCTCCTCCCATCCTGTTTATTGCTATCGGATTGGACAATGGTTGCATTTATTGCATCAAAGGTGATATTGCACGTGAGCGTATCACACGCTTCACATTGCAAGTTGAACCTGTTTCAGACGGCACAAGTTCACCTATTACTGGTCTTGGGTTCCGAGTTGAAGGGCAGGCACATCAGCTCTTTGCCATTACTCCTAGTTCCATAACCTTGTTCAGCTTGCATCATCAACCACCAAGGAGGCAAACACTTGACCAAATTGGTTGTGAGACCAATGCCGTAGCAATGAGTGACCGCATGGATCTGATTGTTGGTAGACCAGAAGCAGTGTATTTCTACGAAGTTGATGGTAGAGGCCCTTGTTGGGCTTTTGATGGTGAGAAGAAGTTTGTGGGTTGGTTTCGGGGTTATTTACTTTGCATAATTGAAGATCAGAGAAGCCGAAAGAATACTCTTAATGTTTACGATCTTAAGAATCGGTTAATTGCGCATAGCATGCCTGTGGGGGATGTTTCACATTTGGTCACTGAGTGGGGCTATATTATTCTCATAATGAGTGATAAAAGGATACTCTGCATTGGGGAGAAAGACATGGAAAGTAAGCTCGACATGTTATTTAAGAAAAATCTTTATACAGTTGCAATCAATCTTGTACAAAGTCAGCAGGCTGATCCTGCTTCAACTGCCGAGGTTCTACGTAAGTATGGTGACCATCTGTATGGAAAACAAGAATATGATGAGGCCATGTCTCAATATATCCACACCATTGGTCATCTTGAACCGTCATATGTTATACAGAAGTTTCTTGATGCAAAGCGTATCCACAACCTGACAAATTATCTAGAAAAGTTACATGATAGAGGATTAGCATCCAAAGACCACACAACCCTTCTGTTGAACTGCTATACCAAATTGAAAGATGTTGAGAAGCTGAACCATTTCATCAAAGATGAAGATGGGGTAGGTGAAATTAAGTTTGATGTGGAAACTGCCATAAGAGTATGTCGTGCAGCTGGATATCATGAACATGCTATGTTTGTGGCCAAAAAGGCTGGGAGACATGAGCTGTATTTGAAGATTTTACTTGAGGATCTTGCTAGATATGATGAGGCGCTGCAGTATATATCTGGCCTTGAGGCAAACCAAGCTGGTCTTACTGTAAAAGAATATGGAAAAATTCTTGTGGACCATAGACCTGCTGAAACTGTTAAAATACTGTTGAGGCTTTGTACTGATGGGGGAGATCCTACGACAAGGAGAGGTTCAAATAGCATGCGCTTGCTTATGATACCTTCACCAATGGACTTTGTAAATATATTTGTGCACAGCCCACAATATCTCATGGAATTTCTAGAAAACTATATCAAAGCAGTCAAGGACTCACCTGCTCAGACAGAAATCCATAACACCCTTCTGGAGCTGTATATATCAAAAGATTTAAGCTTCCCATCTATGTCACAAGAAAATGGCTTTGATGATCACAATAGTAAAGAAAGAAAAGGGAAGGAAATTACAAACGGCTATAAATCAGGCACAAGGGAGAAAGCGAAACTTGGAAAAGAAGAAAATAAAACAGCAAAGGATATTGTTGACAGACAAAGGAAAGGACTTGCTTTGCTGAAGTCTGCCTGGACACCTGAGATGGAAGAACCTTTGTATTCTGTTGATCTTGCTCTTATCATTTGTAATGCAAATGCATTTAAAGATGGCTTGTTATTTCTGTATGAGAAATTAAAACTCTACAAAGAGGTTATCAGTTGCTACAAGCAAGCTCATGACCATGAAGGTTTAATTGCATGCTGTAAGAAGCTAGGGGACTCAACTCAAGGAGGGGATCCATCTCTCTGGGGTGACCTGTTGAATTATTTTGGTGAGCTTGGGGAAGATTGCTCTAAGGAAGTTAAAGAGGTCTTGACCTACGTTGAGAAGGCGGATGTTTTGCCTCCAATTGTTGTCCTACAGACACTATCAAAAAACCCATGCCTGACTCTCTCAGTTGTCAAGGATTACATTGCTCGCAAACTTGAGCAAGAATCAAAGCTAATCGAAGATGACAGAAAATCTGTTGACAAGTACCAGGAAGAGACAGAATTGATGAAAAGGGAGATAGAAGACCTCAAGACAAACGCAAAGGTTTTTCAACTAAGCAAGTGCACAGCATGCACCTTCACCCTTGATCTTCCTGCTGTCCATTTCATGTGCATGCACTCGTTCCATCTTCGCTGCCTTGGGGACAATGAGAAGGAGTGCCCGGAATGTGCACCTGAATACAGATCTGTTATGGAGGCAAAGCAGAAGCTAGAACTAAATGCCAGGGATCATGATCTCTTCTTCAGGCAGTTAAGGGGTTCGAAGGATGGCTTTTCTGTGGTAGCAGACTACTTCAGCAAGGGCGTAGTGAGCAAAACGACGATTCCACCTGAAAATGCCCCATAG
Protein Sequence:
- >TRIUR3_33706-P1|Triticum_urartu|FAR1|TRIUR3_33706-P1
MASPSLASLTTSPRSVPWNQDTYEQSHAPKQRTFTCSRQGHRREDKREETFTYSRPETRCGCDARMKISLRDGFYYVYEFEASHNHTLANGLMAQYLRSQRKVTEAQIANIEVTKSVGISNKATIDLMAKEAGGSENLGFTPQDMKNCLYSKRTIKAKVGDTGGVLEYMEKKVSEDVNFFYSIQVDEDELITNIFWADSKMVSDYAMFGDVICFDTTYRKLDDGRPFGLIVGVNNHKKTIVFGAALLYDETAASFAWLFRTFLKVMSGKHPRTILTDEDAAMAKAIREVFPHSHHRLCVWHMNQNTCKHLAGVVDEYKKFNVDFQHCIYDIEEEDEFVGAWDKMIDKYGLCDNEWLQRLFEKKEHWALVYGKNMFSARMSTTQRSESMNNELKRYISIKYDMLTFFEHFERLVSDKRCEEVKYDFKATQTTPKLKAESSYMLKQAAATYTPAIFKMVQDQVLRTLNYDMILCDESDTEVKIYVVKFHGTQREHVVRFIPKEEKVSCSCKRFEFAGILCSHCLKVLDINNIKHIPGEYILKRWTIDAKVLDITSKCNPHDNLKARMSNCYKELCIIFVKIAARAAESEESYYKAQLAQDVEKCLKIRADPDLVSVDPHLVHVDPNLVHGDPDLVSANQDLVSADPDLGNSITKEGTNIASSRISKHGEGLAKPKGMKVKEKTVKGSKRPIGGFEKATSKKKKNTNDIVHLEPRGPSTGPLKKATLKKKKNTNDIIQLQPHDLPTGHPTNNMYTSLQGTIGASNSGLMTGENQDFLHQPNPASILNASMLQSSGHTIPMPPHIQEYGNTPVQRNVLVTVGDDDQASSQSSAVCLKVFDLDKVQEEGSSTTTPFCVQILRVFTNQFPEAKITSFLVLEEAPPILFIAIGLDNGCIYCIKGDIARERITRFTLQVEPVSDGTSSPITGLGFRVEGQAHQLFAITPSSITLFSLHHQPPRRQTLDQIGCETNAVAMSDRMDLIVGRPEAVYFYEVDGRGPCWAFDGEKKFVGWFRGYLLCIIEDQRSRKNTLNVYDLKNRLIAHSMPVGDVSHLVTEWGYIILIMSDKRILCIGEKDMESKLDMLFKKNLYTVAINLVQSQQADPASTAEVLRKYGDHLYGKQEYDEAMSQYIHTIGHLEPSYVIQKFLDAKRIHNLTNYLEKLHDRGLASKDHTTLLLNCYTKLKDVEKLNHFIKDEDGVGEIKFDVETAIRVCRAAGYHEHAMFVAKKAGRHELYLKILLEDLARYDEALQYISGLEANQAGLTVKEYGKILVDHRPAETVKILLRLCTDGGDPTTRRGSNSMRLLMIPSPMDFVNIFVHSPQYLMEFLENYIKAVKDSPAQTEIHNTLLELYISKDLSFPSMSQENGFDDHNSKERKGKEITNGYKSGTREKAKLGKEENKTAKDIVDRQRKGLALLKSAWTPEMEEPLYSVDLALIICNANAFKDGLLFLYEKLKLYKEVISCYKQAHDHEGLIACCKKLGDSTQGGDPSLWGDLLNYFGELGEDCSKEVKEVLTYVEKADVLPPIVVLQTLSKNPCLTLSVVKDYIARKLEQESKLIEDDRKSVDKYQEETELMKREIEDLKTNAKVFQLSKCTACTFTLDLPAVHFMCMHSFHLRCLGDNEKECPECAPEYRSVMEAKQKLELNARDHDLFFRQLRGSKDGFSVVADYFSKGVVSKTTIPPENAP