Information report for TRIUR3_20720-P1
Gene Details
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Functional Annotation
- Refseq: XP_020182565.1 — protein FAR1-RELATED SEQUENCE 5-like
- Refseq: XP_020182566.1 — protein FAR1-RELATED SEQUENCE 5-like
- Refseq: XP_020182567.1 — protein FAR1-RELATED SEQUENCE 5-like
- Refseq: XP_020182568.1 — protein FAR1-RELATED SEQUENCE 5-like
- TrEMBL: T1MS23 — T1MS23_TRIUA; Uncharacterized protein
- STRING: TRIUR3_20720-P1 — (Triticum urartu)
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Brachypodium distachyon: Bradi4g22525.1.p, Bradi1g48635.1.p, Bradi2g32740.1.p
- Panicum virgatum: Pavir.1NG299700.5.p, Pavir.1NG299700.3.p, Pavir.1NG299700.2.p, Pavir.1NG299700.1.p, Pavir.2KG253800.1.p, Pavir.2NG540200.3.p, Pavir.2NG540200.2.p, Pavir.2NG540200.1.p, Pavir.5NG389600.1.p, Pavir.4KG113400.2.p, Pavir.1KG301400.2.p, Pavir.9NG341400.2.p, Pavir.1KG301400.1.p, Pavir.9NG341400.1.p, Pavir.J172100.1.p
- Triticum aestivum: Traes_2DL_01DEC201E.2, Traes_5BS_86C1C9A9F.2
Sequences
CDS Sequence:
- >TRIUR3_20720-P1|Triticum_urartu|FAR1|TRIUR3_20720-P1
CTGCCCTATTGCCCGCCCCACTCATCTCCTTCCTTCATCTGGCCTGTTGCCTGCTCCATCTTCTCCGCCCCTGATCCAATGCTCGAATCCATGGCAGACAAGCCCACCTGGGTCACCAGTCTCCAGATGAGTCATGGCGAACCTGTACCCTTCATCGAGCCCTCTCCCTCTTTCCTTCCCATGATTAAATCTGCAGCACAGCAGCACACCACTGTTGCCATTGCTGCAACGAACCATCACGAGCCTGCGGTATCTCGCATCCAGTCCGTAGTGGAGCCACAGATTGAAGGGAGATTAGGGGATCTCGATTTGTTCGAGCTAGATAGTTGGCATGGTGGTCAAGACGGCGGTACTGTTGCAGAAACTAGGATATCCTCTCCAATCTCTGTGGTAGCAGACGCCACTGTCCGTGCCAACGAGGATGCGCTAATCCAGAAGACAGAGCCCATCGTACAGCAACCCACGGCGCGCGTCGGCTGCTGGAAACGGAGGAACAAAGGTGTTACAGGGCCTGATGCAAGAGTATGCCCGAGCAAGACAAGTTACCTGCAAGATTGTATTGCAAGAGCGACTGACAATCACAGTAAAGACTTATTTGCCCCCATTGAGGGCATATTGTTCGACACATGCAATGAAGCATATGAGATGTACAATTTGTTTTCATGGGAGAATGGGTTTGGGATTCGGCACTACAAAACAAGGACAAATGGAACAGGGTACAAGGCTATGCACGAGATAGTCTGCCAGTGTCAGGGCAAGAGTGAGAAAGAGAACTCTGAATCTTGTAGAACAGGATGCAAGGCAATGGTGCGCCTCCTTCGTACAAAGGACCATGGGTGGTATGTCTCCTTGTTTGTTAACGAGCACAACCACCCGTTGTCGGCTGGATATGAGGAGACGCGGTATTGGAATTCTCACAGTGTCATAGATCCTTTGTTGAAGGGCTTCATTCGGAACCTAAGGGAGAACAATGTCAGCCTGAGCAAGGTTTACAATATTCTTTGTGTGGCACATGGCGATGGTGCATCTGTACCGTTCCGCAAGGAGAATTTGAAGTACTTGTGCTCCCGTATTGCGCAAGAATCCATAAATGAAGACATATACAAAACAGTTGATCTGCTGCAACAAATGAAGACTGCTGACCCAAACCTGGAGGTGTGTGTAAATGTCGACAAGGAAGGATCTGTTCAGACTATTTTATGGTGCACCGGGAAAAACCGTGAAGATTATGCACACTTTGGCGATGTGGTCACATTTGATACAACTTGCAGGACAAATCTTTATAACATGCCATTCGGCATATTTGTTGGTGTGAATAACCACTTTCAGTCCACTATCTTTGGAGGAGTTCTGATGCGACATGAGAACATTAAGGGGTTTGAGTGGACATTTAGAACCTTTGTCGAGACAATGAATGGCAAGCATCCACAGACAATTCTGACAGACCAATGCAAGGCGATGGAAGGAGCCATAAAGAGTACATTGCCTAACACCCAGCATAGGTGGTGTAAGTGGCATGTATTGAGGTGTGCAAAGGAGAAGCTTGGGCCTGTTTACAGCAAGAACAGTGACTTCAAAAGGGAGTTCCATGCAATAATAAATGAAATTATATGCGTTAAGGAGTTTGAGTGCAAGTGGTCTGAACTTTTACAAAAATTCAATTTGGCGGATAACCTGTACCTAGAAAATTTATATAGCAAG
Protein Sequence:
- >TRIUR3_20720-P1|Triticum_urartu|FAR1|TRIUR3_20720-P1
LPYCPPHSSPSFIWPVACSIFSAPDPMLESMADKPTWVTSLQMSHGEPVPFIEPSPSFLPMIKSAAQQHTTVAIAATNHHEPAVSRIQSVVEPQIEGRLGDLDLFELDSWHGGQDGGTVAETRISSPISVVADATVRANEDALIQKTEPIVQQPTARVGCWKRRNKGVTGPDARVCPSKTSYLQDCIARATDNHSKDLFAPIEGILFDTCNEAYEMYNLFSWENGFGIRHYKTRTNGTGYKAMHEIVCQCQGKSEKENSESCRTGCKAMVRLLRTKDHGWYVSLFVNEHNHPLSAGYEETRYWNSHSVIDPLLKGFIRNLRENNVSLSKVYNILCVAHGDGASVPFRKENLKYLCSRIAQESINEDIYKTVDLLQQMKTADPNLEVCVNVDKEGSVQTILWCTGKNREDYAHFGDVVTFDTTCRTNLYNMPFGIFVGVNNHFQSTIFGGVLMRHENIKGFEWTFRTFVETMNGKHPQTILTDQCKAMEGAIKSTLPNTQHRWCKWHVLRCAKEKLGPVYSKNSDFKREFHAIINEIICVKEFECKWSELLQKFNLADNLYLENLYSK