Information report for Tp57577_TGAC_v2_mRNA34090
Gene Details
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Functional Annotation
- Refseq: XP_024634480.1 — protein FAR1-RELATED SEQUENCE 5-like
- TrEMBL: A0A2Z6PJC0 — A0A2Z6PJC0_TRISU; Uncharacterized protein (Fragment)
- GO:0008270 — Molecular Function — zinc ion binding
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Actinidia chinensis: Achn325861
- Cicer arietinum: XP_012574070.1, XP_004490690.1, XP_012572031.1
- Fragaria vesca: mrna16188.1-v1.0-hybrid, mrna08567.1-v1.0-hybrid
- Malus domestica: MDP0000207355
- Medicago truncatula: Medtr6g090505.1, Medtr0558s0020.1, Medtr1g083640.1, Medtr2g014100.1, Medtr3g008240.1, Medtr4g040430.1, Medtr1g055240.1, Medtr5g071590.1, Medtr1281s0010.1
- Nicotiana tabacum: XP_016513209.1
- Panicum virgatum: Pavir.6NG307100.1.p
- Ziziphus jujuba: XP_015902716.1
Sequences
CDS Sequence:
- >Tp57577_TGAC_v2_mRNA34090|Trifolium_pratense|FAR1|Tp57577_TGAC_v2_mRNA34090
ATGGATTTGACATCAACTTCATCTGGAATTGCCCAAATGCCTGTTGTTGATAGTTCAGACACACTTGCTGATAGTTTGCAATGCTACTATCCTACTAAAAAAGAAACTTGGGAGTCTCTTGCTTTTAAATCGGTTGAAGAGGTTGAAAAATTTTATGGTGATTATGCACTTAAAAGTGGGTTTTCAATTCGGATTTTGTTGAAATCAAGAAACAATTCACAGAATCAGCCAACTGATAAAATACACTATCTACGATATGGATGTAACAAACAAGGTTACAAGAAAGGAAGTTTGCTTGATCCGAAGAACAAGCCAAAATCAGAGAGTCCGGTTGTGGTTGAATTTGAGAANNNNNNNNNNNNNNNNNNNNNNGAAAAACCTGAGGAAAGGGTTGGTTGTACAGCTGCCATATTTTTGAAGTTGGATGAAACTCTGAATGTCTTTAAGATATACAAATGGGATGTGCCCCATTGTCATCCGTTACATAAACCGAAACATGGATGTTACTTGAGATCATTCAGACGAGTCAATGAAGTGCAAGGACAGCTTGCTGTAATAAATTCGAAAGCCGGGATGTCGATGAGAACTTCTTATGAAGTCATGGGTCAGGGAGTTGGAGGAACAGATAACTTGCCTTTTCGATTTTCGGATTTAAAGAATTATCTGATGACAAATCGGCAAAAAGAGATGCTAGTTGGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNAAGATTTTTTTAGAAATGAAGCTCTGTCATTGGTAANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTGTAACTTTGATATATGCTTTTTTTTGTTCTGATTTGGTTCATTTTTACATTTCAGCTGCATTCATGGGCTTTGACAATCATCGTACAAGTGTGTTATTTGGCGCTGCCCTGTTGTATGACGAGACTGCAACAACTTTTGACTGGTTGTTTACAACATTTTTGAAATATTTTGATTACAATTGGGATCAGATGATGAAAACCTGTTTTGATGGAAGGCCAACTGCAGACTTTAAGTGGCTTGTTCAAACTTACAGGAATCGTATGCATTGGTCTTCAGCTTGGGTCAAATCTCATTTTACTGCTGGTTTGAAAACAACTCAATTAAGTGAGTCCTTCAATGCCTTTCTCCGTCGTTTTCTGCAGCCTGATCATTCACTTGTTATATTCTTCAATCATTTTAATATTATGGTCCAGAGAATGAGGGATAATCATACTGAGTTAGACTTTAAGGCTGCAAACACTAGAGCAAAAAACAATTATCCCANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTTTCCCCAAACTTTGAAAAGCATGATCGACTTGATGAACGGCTTGTGACAATTGATATTAGGGCCAAACGTATTAGCTGCACATGCCGTATGTTTGAGAACAGGGGTTTCTTGTGTCGACATGTTTTCAAAATATTGGAGTTTTTCGGTGGTTCTGTGCAATATCATGGTTTGAAGACAATACCTGCAGAATATGTGTTAAAGCGTTGGTGTAGAGATGTGCGTCAGTCTGTTAAATCTACTATCAATGTTGCCACTGAAGGCGCGACTCAAGCACAACGATATCAACAAATTTGTGCTGTTACAGTTAAGCTTTGTACACGTGTTTGTGCAGATCCTGAGGCTTCTCAAATTTTTCTTAATGGTGTTCTTGAAGCTGGGAGAAAGGCAGAGGAGTTGCTTACTTCGAAAGGTATTCATACAGTTCAATCTTCCGTGACGCCATCTAAGTCATCTTCCGTTACGCCATCTAAGTCTTGCAATACAGCAGCTGTCAGTGAAGCATCTGATGCTCAAAAGTCGACTGGTCCAAAGTTCAAAAAAAGACCAAATCCAATTAGATCAAAGAAACGACTTAAAAGTGATTATGAATTGGCTAGAGAATACCAGAAATTCCTTATACACCGGAAGAGAAAACAGAGAGGGGCTGAGGATTTAAAGGGGAAAGAGGCTGCTGATTGA
Protein Sequence:
- >Tp57577_TGAC_v2_mRNA34090|Trifolium_pratense|FAR1|Tp57577_TGAC_v2_mRNA34090
MDLTSTSSGIAQMPVVDSSDTLADSLQCYYPTKKETWESLAFKSVEEVEKFYGDYALKSGFSIRILLKSRNNSQNQPTDKIHYLRYGCNKQGYKKGSLLDPKNKPKSESPVVVEFEXXXXXXXXEKPEERVGCTAAIFLKLDETLNVFKIYKWDVPHCHPLHKPKHGCYLRSFRRVNEVQGQLAVINSKAGMSMRTSYEVMGQGVGGTDNLPFRFSDLKNYLMTNRQKEMLVGXXXXXXXXXXXXXXDFFRNEALSLVXXXXXXXXXXXVTLIYAFFCSDLVHFYISAAFMGFDNHRTSVLFGAALLYDETATTFDWLFTTFLKYFDYNWDQMMKTCFDGRPTADFKWLVQTYRNRMHWSSAWVKSHFTAGLKTTQLSESFNAFLRRFLQPDHSLVIFFNHFNIMVQRMRDNHTELDFKAANTRAKNNYPXXXXXXXXXXXXXXXXXXXXXXXXLSPNFEKHDRLDERLVTIDIRAKRISCTCRMFENRGFLCRHVFKILEFFGGSVQYHGLKTIPAEYVLKRWCRDVRQSVKSTINVATEGATQAQRYQQICAVTVKLCTRVCADPEASQIFLNGVLEAGRKAEELLTSKGIHTVQSSVTPSKSSSVTPSKSCNTAAVSEASDAQKSTGPKFKKRPNPIRSKKRLKSDYELAREYQKFLIHRKRKQRGAEDLKGKEAAD*