Information report for Tp57577_TGAC_v2_mRNA18129
Gene Details
|
|
Functional Annotation
- Refseq: XP_027192103.1 — uncharacterized protein LOC101503771
- Swissprot: Q9SSQ4 — FRS6_ARATH; Protein FAR1-RELATED SEQUENCE 6
- TrEMBL: A0A2Z6N3Y3 — A0A2Z6N3Y3_TRISU; Uncharacterized protein
- STRING: XP_004486559.1 — (Cicer arietinum)
- GO:0008270 — Molecular Function — zinc ion binding
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Actinidia chinensis: Achn097861
- Arachis hypogaea: Ahy018394
- Cicer arietinum: XP_012569658.1
- Glycine max: Glyma.15G125400.1.p, Glyma.09G019300.2.p, Glyma.09G019300.1.p
- Gossypium arboreum: Cotton_A_07486_BGI-A2_v1.0
- Lotus japonicus: Lj6g3v1692830.1, Lj4g3v0070880.1
- Malus domestica: MDP0000257646
- Manihot esculenta: Manes.04G111100.1.p
- Populus trichocarpa: Potri.003G031400.1
- Prunus mume: XP_008228719.2
- Prunus persica: Prupe.3G090400.1.p, Prupe.3G090400.2.p
- Pyrus bretschneideri: Pbr026780.1
- Vitis vinifera: GSVIVT01017368001
- Ziziphus jujuba: XP_015870227.1, XP_015870221.1
Sequences
CDS Sequence:
- >Tp57577_TGAC_v2_mRNA18129|Trifolium_pratense|FAR1|Tp57577_TGAC_v2_mRNA18129
ATGATGTTGGAGTTCCAGACCCAAAATCTTGTTACCATCGACGCCGCCGTCGATTCTCAACCTCCACGAGAAGAAGAACAAGAACAACACTTAAAGATGGACGATACTTCTCTGTACTGCGATCAAATACCAGATGCAGATGATGTACTAATTGAATTAGACTGTCAAAATGATCCTTCTGAAGGAAGAAAGGAATTTCTCGCACCTGCTAGTAGAATGGAATTTGAATCATACGATGATGCTTACAATTATTATGTTTGTTATGCCAAGGAGGTGGGATTTTGTGTGAGGGTGAAGAATTCATGGTTCAAGAGAAATAGTAGAGAAAAATACGGTGCAGTGCTTTGCTGTAGCAGTCAAGGTTTCAAAAGAATAAAAGATGTTAACAGTTTCAGAAAAGAGACAAGAACTGGTTGTCCTGCGATGATAAGGATGGGGTTAGTGGAGTCTCAGAGATGGAGGATACACGAGGTTACCCTCGAACATAACCACATCTTAGGTGCTACGATACATAAATCAATTAAAAATGTGGGAATTGGGACTAAAAAGAAGTTGTTGCCTAGTTGTTCAACTGCTGAGGTTCAAACAGTTAAGTTATATCGTGCACTTGTGATAGATGCGGGAGGTAATGGTAATTCAAATTCCAATGCAAGAGACAATAAAACTTTCTCTGAATATTCTAATGAGTTGAATCTAAAAAAAGGTGACGTGCAAACCATTTATAATTTCCTTTGCCGTATGCAACTGACTAATCCAAACTTCTTTTATTTAATGGATTTCAACGATGAAGGGCATTTGAGGAATGTATTATGGGTTGATGCCAGGTCCAAGACTGCATGCGGTTATTTTGGTGATGTTATTTATTTTGACAACACTTATTTGTCAAACAAATATGAAATTCCTCTTGTGGCTTTTGTCGGAATAAATCACCATGGTCAAACAGTGTTATTAGGTTGTGGGCTGCTTGCAGGTGAAACGATCGAATCATATACATGGTTGTTTAGAACATGGGTTATGTGTATGTCAGGATGTTCTCCTCAAACTATTATTACAGATAGGTGCAGGGCTTTGCAGATTGCAATTGCAGAAATTTTTCCGAAATCTCATCATTGTTTTGGCTTGTCATTAATTATGAAAAAAGTACCTGAAAAATTGGGAGGGTTGCACAATTACGATGCAATCAGGAAAGCATTGATTAAAGCAGTTTATGAGACATTGAAAGTGATTGAATTCGAGGCAGCATGGGGGGTTATGATTCATTGTTTTGGAGTTAGCGATCACGAGTGGCTTCGTTCCTTATATGAAGACCGAGTTCATTGGGCACCAGTATATTTAAAGGACAAGTTTTTTGGTGGAATGGCTGCGGCACGTCCCGGTGAGAGTGTGTGTCCATTTTTCGATAAATATGTGCACAAACAGACTCCTTTGAAGGAGTTTCTTGACAAGTATGAATTAGCTCTTCATCAGAAGTACAAGGAAGAATCTTTAGCAGATATTGAATCAAGAAGTTCAAGCCCCTTGTTAATAACCAAATGTTCTTTTGAACTGCAGCTTTCTAGAATGTACACAAAAGAAATATTCACGAAGTTCCAATTCGAAGTGGGAGAAATGTTCTCATGTTTTGGTACGACTCAACTACACGTGGATGGTCCCATCATAATCTTCTTGGTCAAAGAGCGTGTTTTGACCGATGGAAACAGACGGGAGATTAAGGATTTCGAAGTTTTGTACAATAGATCAGCAGGTGAAGTTCGTTGTATTTGTTGTTGCTTTAACTTTTATGGATATCTATGTCGACATGCACTGTGCGTACTCAATTTTAATGGGGTGGAAGAGATTCCGCCTAAGTACATTCTTTCACGGTGGAAGAAAGATTACAAACGTCTTTATATTCCTGATCACAATTTTGGCAGTTCTGATGATACTGATCGTATTCACCGATCTAATCAGTTATTTAGAAGTGCCTTACAAGTTGTAGAGGAGGGAATACTTTCTGTAGACCATTACAATGTAGCGTTGCAGGCTTTTGAAGAATCGTTTATTAAGGCCCGTGATGTAGAGCAAAGAAATGAGTAA
Protein Sequence:
- >Tp57577_TGAC_v2_mRNA18129|Trifolium_pratense|FAR1|Tp57577_TGAC_v2_mRNA18129
MMLEFQTQNLVTIDAAVDSQPPREEEQEQHLKMDDTSLYCDQIPDADDVLIELDCQNDPSEGRKEFLAPASRMEFESYDDAYNYYVCYAKEVGFCVRVKNSWFKRNSREKYGAVLCCSSQGFKRIKDVNSFRKETRTGCPAMIRMGLVESQRWRIHEVTLEHNHILGATIHKSIKNVGIGTKKKLLPSCSTAEVQTVKLYRALVIDAGGNGNSNSNARDNKTFSEYSNELNLKKGDVQTIYNFLCRMQLTNPNFFYLMDFNDEGHLRNVLWVDARSKTACGYFGDVIYFDNTYLSNKYEIPLVAFVGINHHGQTVLLGCGLLAGETIESYTWLFRTWVMCMSGCSPQTIITDRCRALQIAIAEIFPKSHHCFGLSLIMKKVPEKLGGLHNYDAIRKALIKAVYETLKVIEFEAAWGVMIHCFGVSDHEWLRSLYEDRVHWAPVYLKDKFFGGMAAARPGESVCPFFDKYVHKQTPLKEFLDKYELALHQKYKEESLADIESRSSSPLLITKCSFELQLSRMYTKEIFTKFQFEVGEMFSCFGTTQLHVDGPIIIFLVKERVLTDGNRREIKDFEVLYNRSAGEVRCICCCFNFYGYLCRHALCVLNFNGVEEIPPKYILSRWKKDYKRLYIPDHNFGSSDDTDRIHRSNQLFRSALQVVEEGILSVDHYNVALQAFEESFIKARDVEQRNE*