Information report for SMil_00001536-RA_Salv
Gene Details
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Functional Annotation
- Refseq: XP_011100396.1 — protein FAR-RED ELONGATED HYPOCOTYL 3 isoform X1
- Swissprot: Q9LIE5 — FHY3_ARATH; Protein FAR-RED ELONGATED HYPOCOTYL 3
- TrEMBL: A0A2P5Q0G2 — A0A2P5Q0G2_GOSBA; Uncharacterized protein
- STRING: Migut.M00094.1.p — (Erythranthe guttata)
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Juglans regia: WALNUT_00003351-RA
- Nicotiana benthamiana: Niben101Scf04464g02002.1
- Nicotiana tabacum: XP_016449482.1, XP_016449480.1, XP_016449479.1, XP_016449478.1, XP_016449477.1, XP_016449476.1, XP_016481999.1, XP_016481998.1, XP_016481996.1
- Sesamum indicum: XP_011100396.1, XP_011100397.1
Sequences
CDS Sequence:
- >SMil_00001536-RA_Salv|Salvia_miltiorrhiza|FAR1|SMil_00001536-RA_Salv
ATGCGTTTGTTTGTACTTACCTTCGTGATGTTGTATGCCTCTGCACATGACAGAATCATGAATGTGGTGGTTGCTACACAACACCTTATTGCAGGACAACGAGTTGTTGTCATGGATATAGATCTTAGGTTGCCTTCTGGAGAACATGATAAAGAATTAGAAGAGCCTAATGGAATTGTTAATATGCTAGATGGGGAAGAGAAGCCCCTCAATGTAGATGGAGTGGGTGAGAGTATGGGAGACGAGGATAAGCTGCATGTCGAAGATGGAGATGATGTGAATTCTCCCTTACCTGATATAGATTTCAAAGATGTGACAATTCTTGAGCCACTTCCTGGTATGGAATTTGGGTCTCACGGGGATGCCTATGCTTTCTATCAGGAATATGCCAGATCTGTGGGATTCAATACTGCAATTCAGAATAGCCGCCGGTCAAAAACATCAAGGGAATTCATTGACGCAAAATTCGCATGCTCTAGATATGGCACAAAGCGTGAGTATGAGAAATCTTTGAACCGCCCGCGCAGTAGACAAGGAAGCAATCAGGATGCTGAGAATGCTACAGGTCGTCGAGCATGTGCAAAGACAGATTGTAAAGCAAGTATGCACGTTAAGAGAAGGTCCGATGGGAAATGGATAATCCATAGATTTGAGAAAGAGCATAACCATGAACTGTTACCTGCCCAAGCTTCGGTCTTAAACATGACTCCAGAAGCCAATTCGAAAAAGGCAGGAATATGGCAATGGATGCAGTTCCTTTGTAGACATGCAATGATTGTTCTTCAAATCTGTGGCATCTCAACAATACCGTTGCAATATATCTTGAAACGGTGGACGAAAGATGCCAAGAGCAGATACTCAATCGAAGGGTCTGAACAGGTGCAGTCAAGGCTGCAGCGTCTGTCTCTTCGTGCACTTGGTGATGCTTTTGAGAACTGTTTGAATGCGAACTACTCCAATAAGAATCTTCTAGAAGCTGGACCTTCTGCCTCCCCAGGTATTTTGTGCATCGAAGACGACATTCAGAGTGGAAGTTTGAGCAAGACGAATAAGAAGAAGAATGCAACTAAGAAAAGAAAGGAAAAATTGAGTTCTCGACCTGTAAATCTGGACGGATTTTTCGGTCATCATCAAAGCGCTCCGGGAATGGTACAGCTGAACTTAATGGGTCCTACACGTGATAGTTACTTCGGGAACCAACCTGCCATTCAGGGACTAGGCCAGCTGAACTCCATTGCACCGACCCATGATGGTTACTATGGTTCTCAGCCTGCCATGCCCGGAATGGGGCATATGGAATTTTTCCGCACTCCAAATTTCGGCTATGGTATTCGGGATGATCCCAACGTGAGATCTGCGCAGTTGCATGATGACGCAACAAGACACGCGTGA
Protein Sequence:
- >SMil_00001536-RA_Salv|Salvia_miltiorrhiza|FAR1|SMil_00001536-RA_Salv
MRLFVLTFVMLYASAHDRIMNVVVATQHLIAGQRVVVMDIDLRLPSGEHDKELEEPNGIVNMLDGEEKPLNVDGVGESMGDEDKLHVEDGDDVNSPLPDIDFKDVTILEPLPGMEFGSHGDAYAFYQEYARSVGFNTAIQNSRRSKTSREFIDAKFACSRYGTKREYEKSLNRPRSRQGSNQDAENATGRRACAKTDCKASMHVKRRSDGKWIIHRFEKEHNHELLPAQASVLNMTPEANSKKAGIWQWMQFLCRHAMIVLQICGISTIPLQYILKRWTKDAKSRYSIEGSEQVQSRLQRLSLRALGDAFENCLNANYSNKNLLEAGPSASPGILCIEDDIQSGSLSKTNKKKNATKKRKEKLSSRPVNLDGFFGHHQSAPGMVQLNLMGPTRDSYFGNQPAIQGLGQLNSIAPTHDGYYGSQPAMPGMGHMEFFRTPNFGYGIRDDPNVRSAQLHDDATRHA