Information report for NNU_004563-RA
Gene Details
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Functional Annotation
- Refseq: XP_010251622.1 — PREDICTED: protein FAR1-RELATED SEQUENCE 3
- Swissprot: Q9ZVC9 — FRS3_ARATH; Protein FAR1-RELATED SEQUENCE 3
- TrEMBL: A0A1U7ZFE8 — A0A1U7ZFE8_NELNU; protein FAR1-RELATED SEQUENCE 3
- STRING: XP_010251622.1 — (Nelumbo nucifera)
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Cucumis sativus: Cucsa.142680.1
- Juglans regia: WALNUT_00006432-RA
- Manihot esculenta: Manes.11G111500.2.p, Manes.11G111500.1.p
- Petunia inflata: Peinf101Scf08365g00008.1
- Populus trichocarpa: Potri.009G158400.2, Potri.009G158400.1, Potri.004G196300.3, Potri.004G196300.2, Potri.004G196300.1
- Ricinus communis: 29942.m000750
- Salvia miltiorrhiza: SMil_00007899-RA_Salv
- Solanum melongena: Sme2.5_01096.1_g00012.1
- Vitis vinifera: GSVIVT01037455001
- Ziziphus jujuba: XP_015873302.1
Sequences
CDS Sequence:
- >NNU_004563-RA|Nelumbo_nucifera|FAR1|NNU_004563-RA
ATGGATGTTGAAGTGATTGACGTGGAAGAGGAGAATATGGCACGCCGTGCAGCAGCAGAAGATGCAGATTCTGAGCCAAATGAGAGTGGAGAAATGAAAATGAATGAAAATTCTACAGAGAAAGAGGCACCCTCTCAAGAGGAGGATGGAAGTGGTGAACCCCATGTTGGTATGGAGTTTGAATCAGAAGATTCTGCAAAGGCATTCTATGATGCATATGCCAGGCGTATTGGTTTCAGCACCCGTGTTGGTCAATGCAACCGTTCAAAGCCTGATGGAACAATTACATCTCGGGAGTTTGTATGTTCTAGGGAGAGTTTGAAAAGGAAGAATATTGAGAGCTGTAGTGCGATGCTTAAGATTGAAAGAAAGGACCCAGACAAGTGGGTTGTGACAAAGTTTGTCAAGGAGCATAACCACTCTACAGTGAGTCCCAGTAAGGTGCATTACCTCCGGCCCCGCAGGCATTTTGCTGGTGCAGGGAAGGAAGGTTACCAAGGGGTGCAGATTCTACCAAGTGGTGTTATATATGCTGAGGAAGGAGCAATAGCTGTAGAGACTTACAATGTGGCAATGAATTCTCTAAGAGAGGGTGGAAAGAAAGTTTCTGTTGTGAAGAAAAATGTTGCAAGAGTTGCACCTCCTAGCTCTCAGGTCATTGGAAGTAACCAGGATGAAATTAGCCGGAGAACACCTATACCACCTACAGAGATGACTCCATCATTGTGGCCTCGACAAGATGATGTGACCCGTCACTTTAATCTTAATGATACTGGTGCCCCTACTCCACCTGTTGCAGACTTGAATCTACCAAGGATGGCCCCTGTCTCTCTTCATCGGGACGATGGTCCTTCTGATAACATGGTTGTGCTTCCTTGCCTCAAATCAATGACTTGGGTCATGGAGAATAAGAACTCTCTGGCTAATAGGGTAGCTGTTATAAATTTGAAGTTGCAAGATTATAGCAAGACTCCTTCTGGAGAAACAGAAGTAAAATTTCAACTTTCAAGGGTCACACTAGAACCAATGTTGAGATCTATGGCTTACATCAGCGAGCAGCTCTCAACACCTGCTAATAGGGTTGCTGTTATCAATCTGAAGCTCCAAGATACTGAGACAACTACTGGAGAAACCGAGGTGAAGTTTCAGGTATCCAGAGATACGTTGGGTGCAATGTTGAGATCAATGGCCTATATTCGTGAGCAGCTGTCAAATGCTTCTGATTGA
Protein Sequence:
- >NNU_004563-RA|Nelumbo_nucifera|FAR1|NNU_004563-RA
MDVEVIDVEEENMARRAAAEDADSEPNESGEMKMNENSTEKEAPSQEEDGSGEPHVGMEFESEDSAKAFYDAYARRIGFSTRVGQCNRSKPDGTITSREFVCSRESLKRKNIESCSAMLKIERKDPDKWVVTKFVKEHNHSTVSPSKVHYLRPRRHFAGAGKEGYQGVQILPSGVIYAEEGAIAVETYNVAMNSLREGGKKVSVVKKNVARVAPPSSQVIGSNQDEISRRTPIPPTEMTPSLWPRQDDVTRHFNLNDTGAPTPPVADLNLPRMAPVSLHRDDGPSDNMVVLPCLKSMTWVMENKNSLANRVAVINLKLQDYSKTPSGETEVKFQLSRVTLEPMLRSMAYISEQLSTPANRVAVINLKLQDTETTTGETEVKFQVSRDTLGAMLRSMAYIREQLSNASD