Information report for MELO3C007004P1
Gene Details
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Functional Annotation
- Refseq: XP_008439434.1 — PREDICTED: protein FAR1-RELATED SEQUENCE 4 isoform X1
- Swissprot: Q6NQJ7 — FRS4_ARATH; Protein FAR1-RELATED SEQUENCE 4
- TrEMBL: A0A1S3AYS5 — A0A1S3AYS5_CUCME; protein FAR1-RELATED SEQUENCE 4 isoform X1
- STRING: XP_008439433.1 — (Cucumis melo)
- GO:0008270 — Molecular Function — zinc ion binding
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Actinidia chinensis: Achn346681, Achn018201
- Cicer arietinum: XP_004486947.1
- Cucumis sativus: Cucsa.048930.1, Cucsa.048930.2, Cucsa.048930.3
- Fragaria vesca: mrna12562.1-v1.0-hybrid
- Nicotiana tabacum: XP_016491102.1, XP_016491095.1, XP_016491088.1, XP_016491135.1, XP_016491107.1, XP_016491131.1, XP_016491126.1, XP_016491120.1
- Populus trichocarpa: Potri.003G207100.2, Potri.003G207100.1
- Prunus mume: XP_008234548.1, XP_008234547.1, XP_008234549.1
- Prunus persica: Prupe.2G327900.1.p
Sequences
CDS Sequence:
- >MELO3C007004P1|Cucumis_melo|FAR1|MELO3C007004P1
ATGGATTCCAGTTCCATCATCACCAATTCTTTCCAAGAGCCATGCCTTGGCATGGAGTTTGATTCTCACGAACATGCATATTCATTCTATAGAGACTACGCCAAGACTATGGGCTTTGGAACTTCCAAATTAAGCAGCCGTCGTTCTAGGGCATCCAAGGAATTTATTGATGCAAAGTTTTCATGCATGAGATATGGCAATAAGCAGCAGTCTGATGATGCTATCAACCCACGTCCTTCCCCCAAAATTGGTTGTAAAGCCAGCATGCACGTCAAGAGAAAAAATAATGGCAAATGGTATGTTTATAGTTTTGTGAAAGATCATAACCACGACCTTTTACCTTCTCAAGTTCATCTTTTTAGAAGCCACCGAAATATTGATCCACTCAAAAACGACGTTAGAATACGGAGACGGAAGAATTTAGCTGCCATCTCAAAACTGTTCAGTGCTTATCAGAATGTTGATTGTTTAGAAAGCTTTGTGAGAAACCAACACGATAAGGGTCGCACATTGGTTCTAGAATCAGGGGACGCGCATATTCTGCTTGAACTTTTTATGCACATGCAGCAAGAAAATCCAAAATTCTTTTATGCAGTTGATATGAATGAAGAGCACCAGTTGCGCAATGTGTTCTGGGTTGACGGCAAAGCAATGGGAGAACGAGCTCCAAAGGTGATACTCACTGACCAAAATACCTCTATTAAAGCAGTCATTGGGGCAGTTCTTCCTGGAACACGTCACTACTTTTCTCTGTGGTATATTTTGGAAAAGATTCCTAAAGAGCTTGAGTTTTTAAGCATGTGGCACGAGAATTTTATGGAGAAGTTCAAGAAGTATGTTTTTAAGTCGTGGACCAAGGAAGAGTTTGAGAAGAGGTGGCAGAAGTTGCTCGATAGATTTAATCTTAGAGAAGTTGAGTGGATGCAACATTTGTATGATGATCGTGCTTATTGGGTCCCTGCTTTTGCAAGAGATGTTTCTTTTGCTGGCTTATGCACATCTTCACGAATGGAGAGTTTGAACTCTTCTTTTGACAAATATGTTCAGATTGAAACATCCTTGAAGGAGTTTATAGTTCGATACAGAGACATTCTTGAGGAGCGATATGAGGAAGAAGCAAAGGCAAATTTTGATGCTTGGCATGAATCACCGGAGTTGAAGTCTCCATCTCCATTTGAGAAACAAATGTCACTGGTATATACTTACGAAATCTTTAAAAAGTTTCAAATGGAGGTTTTGGGAGCTGCTGCGTGCCATCTGAAGAAAGAAACTGAAGACGAAACTCTTGCGACTTACAATGTCAAAGACTTTGAAGACGGTCAGAATTATGTTGTTGAATGCAGTCACTCAAATTCAGATATATATTGCTCCTGCCGTTCATTTGAATATAAAGGTTTTCTCTGTAGACATGCCATTATTGTCCTCCAAATGTCTGGCGTTTTCAGTATACCATCCAAGTATATATTGCAGCGTTGGACCAACACTGCTATGAGCAGAAATCCAATCAATGAAAAGTTGGATGAAGTGCAATGTAAGGTCCGTCGTTTTAATGATCTTTGTCGAAGAGCTATAATATTGGGAGAAGAAGGGTCTTTGTCTCAGGAGAGTTATGACATTGCACTTTCTGCAATTAATGAAGCTTTGAAACAATGTGCAACTGTGAGTAGGAGCAGTTCTGCAGAAAGTGATGTGAGGTCTGACACCTCAGCAATGCTTGTTTTTGGTATTGAAGATAACCAATGCAACAACAACCTAGCTGTTGATAATGCCCCTGATCTTAAGGTGATTAATGCTAAAAAAATTCCTAACCTGGCAGGGTCGAGCAATGAACCTGCAGTCAATGAAAGCAACAAAAATAGGAAGGTATCTCAACCATTTGCTACAAATGCTGGATCCCGGGATGATTTCAATCAAATGGAGTTGAGTGATATGCGGCCAATCCAATTGAATGGAATATCACCGACGCAATTGCATAACATGGTACCAACACTGCTTCAGTTTCATAGCATGTCTTCTTCCCATTTGCACGAGGATCGTCTTCCTCGATGA
Protein Sequence:
- >MELO3C007004P1|Cucumis_melo|FAR1|MELO3C007004P1
MDSSSIITNSFQEPCLGMEFDSHEHAYSFYRDYAKTMGFGTSKLSSRRSRASKEFIDAKFSCMRYGNKQQSDDAINPRPSPKIGCKASMHVKRKNNGKWYVYSFVKDHNHDLLPSQVHLFRSHRNIDPLKNDVRIRRRKNLAAISKLFSAYQNVDCLESFVRNQHDKGRTLVLESGDAHILLELFMHMQQENPKFFYAVDMNEEHQLRNVFWVDGKAMGERAPKVILTDQNTSIKAVIGAVLPGTRHYFSLWYILEKIPKELEFLSMWHENFMEKFKKYVFKSWTKEEFEKRWQKLLDRFNLREVEWMQHLYDDRAYWVPAFARDVSFAGLCTSSRMESLNSSFDKYVQIETSLKEFIVRYRDILEERYEEEAKANFDAWHESPELKSPSPFEKQMSLVYTYEIFKKFQMEVLGAAACHLKKETEDETLATYNVKDFEDGQNYVVECSHSNSDIYCSCRSFEYKGFLCRHAIIVLQMSGVFSIPSKYILQRWTNTAMSRNPINEKLDEVQCKVRRFNDLCRRAIILGEEGSLSQESYDIALSAINEALKQCATVSRSSSAESDVRSDTSAMLVFGIEDNQCNNNLAVDNAPDLKVINAKKIPNLAGSSNEPAVNESNKNRKVSQPFATNAGSRDDFNQMELSDMRPIQLNGISPTQLHNMVPTLLQFHSMSSSHLHEDRLPR*