Information report for MDP0000285475
Gene Details
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Functional Annotation
- Refseq: XP_028958052.1 — protein FAR1-RELATED SEQUENCE 6-like
- TrEMBL: A0A251MR68 — A0A251MR68_PRUPE; Uncharacterized protein
- STRING: XP_008381561.1 — (Malus domestica)
- GO:0008270 — Molecular Function — zinc ion binding
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Actinidia chinensis: Achn078441, Achn046071, Achn275581, Achn163291
- Brachypodium distachyon: Bradi3g51320.1.p, Bradi2g36810.2.p
- Cicer arietinum: XP_004496070.1
- Glycine max: Glyma.08G172600.3.p, Glyma.08G172600.2.p, Glyma.08G172600.1.p
- Prunus mume: XP_008227658.1
- Prunus persica: Prupe.8G014900.1.p, Prupe.4G256500.1.p
- Triticum aestivum: Traes_1AS_F08B3720F.2, Traes_4AS_6B1AEFACE.2, Traes_4BL_F0B0E6B0A.2
- Ziziphus jujuba: XP_015893354.1
Sequences
CDS Sequence:
- >MDP0000285475|Malus_domestica|FAR1|MDP0000285475
ATGACACTGGGGGTTGAAGAAGAACCCGAAAAATTGGAGGACACTAACCACGTGATGATTATTAGCAGCCCCATATATGAAGGAACAGGGAGTTCTTCTAATGAATTTACTGAAGAAGATGATGTAGTTGAAATGAGTTCTTGTAATGAAACTGAAAGCGATGGGGTTCATACAATTGAAACTTGTACTGAAAGTACTGAAGAAGATGATGTAATTGAAGCAACTTTTGATGCCACTGAAGTAGAAAGCAATGGGGTTCACGCAGTGGAAAATAATGAGATTAACATGGAGAACACAATGGATGTGGAAGAAAAGGTCCAAGAGCCTCAAGTTGGAATGATCTTTGACAACGCGGATCAAGTGGCTACATATTACAAGGAATATGGGAGACAATCAGGATTTCCAATGATAAAGAGATCATCAACGAAGGGAGATGATGGAAAATTGAGGTATATTACTATGTGTTGCGCTCGATCAGGTACATCAAAAAATACTTCAAGTAATCCTCTTAAGCCATATCCAAGTATAAAGAATGATTGCAAAGCTCAACTAAGGGCAGGTTTATACTTGGGTGAGAAGTGGCGAGTGAATTCTGTAAAGCTTGACCATAATCATGGGTTGAGTCCAACAAAAACTCGTTATTGGAAGTGTTTTCGGGAAGTTAGTTCTTTAGTAAAAAAGAAGCTTGAAGTGAATGATAAAGCTGGAATAAGGGTCAACAAAAGTTATAATTCAGTGGTGGTTGAAGTTGGAGGGCATGAGAACATGAAGTGTTTAGAAAAAGACTGTAGAAATTATATTGAAAAGGTAAGGCGCTTACGACTTAGAGAAGGAGATGCCACTGCAGTTCAAAAMTACTTTGTAAGCATGCAAGGTCAGAATGCAAATTTCTTCTATGCAATTGATTTGGACAAAAATGGTCGTTTAAGAAATATATTTTGGGCAGATGCAAGGAGTAGGGCGACATATGAAGAATTTGGTGATGTTGTAACATTTGACACAACGTACTTGACTAATAAGTACGATATGCCATTTGCTCCATTTGTGGGAGTTAATCATCACAGGCAATCAATTTTATTGGGATGTGGATTGATTTCAAGGGAAGATAATGACTCCTTTATTTGGTTGTTTAGGACCTGGCTTGCTTGTATGTCTGGTCATGCTCCAAGTGGAATAATTACAGATCAAGACAAAGCTATGAAAAAAGCGATTGAAGTTGTTTTTCCTAATACTCGACATCGGTTGTGCTTGTGGCATATAATGAAAAAGGTTCCTGATAAGCTCAAAAGTTATAAGGAATATGAATCCATGAGCTCTTCGTTAGATAACATTGTTTATGACTCAATAACTTCTGAAGATTTTGAGGAACGTGGGGCTGAGATGATTGGAAAATATAAGTTTAAAAAGCAAGCACGTAAATGGTTAAAAAGCTTGTATGCAGAGAGACATCATTGGGTGCCTGTGTATGTGAAAAATAAATTTTGGGCTGGAATGTCAACGACACAAAGGAGTGAGAGCATGAATGCATTTTTTGATGGCCACGTGAACTCAAAGACTACTTTAAAACAATTTCTGGAGCAATATGAGAATGCATTAAGGAGTAAAGTGCTAAAAGAGGCCTCTGCAGATTCTGATTCTTTCTCTTCAAATGTTCGTTGTGCAACTCATTATGACATGGAAAAGCAAGTTCAAGCTGTTTACACGATTTCAAAATTCAAGGAGTTCCAAAAGGAATTGACAAGTATAATGTATTGTGACAGGGTTTCAGTTGAAACTGATAATGCAATTTTGGAGTACCAAATAGCTGAGCGTATTCTGATCAAGGAGAACAAAAAGAAGACTGTCATTTTCAAGGTCCAGTTTAATGAGCGTGAGATTGAAGTTAATTGCAATTGCTGCAAGTTTGAGTTTAGAGGTATATTATGTAGACATGCGCTTTATGTTTTAGTTCATCATGACATTGACTTTATGCCAGATAAGTATATCATTCGGAGATGGAGGAAGGATGTGAAAAGATGCCACACAAGGGTTAGGATAAGTTATAGTAATTGGGTTGCTACACCTGAAGCACAAAGATCTGATATGATGCARAAACCTTTGATGACATTAAGGAATTAG
Protein Sequence:
- >MDP0000285475|Malus_domestica|FAR1|MDP0000285475
MTLGVEEEPEKLEDTNHVMIISSPIYEGTGSSSNEFTEEDDVVEMSSCNETESDGVHTIETCTESTEEDDVIEATFDATEVESNGVHAVENNEINMENTMDVEEKVQEPQVGMIFDNADQVATYYKEYGRQSGFPMIKRSSTKGDDGKLRYITMCCARSGTSKNTSSNPLKPYPSIKNDCKAQLRAGLYLGEKWRVNSVKLDHNHGLSPTKTRYWKCFREVSSLVKKKLEVNDKAGIRVNKSYNSVVVEVGGHENMKCLEKDCRNYIEKVRRLRLREGDATAVQXYFVSMQGQNANFFYAIDLDKNGRLRNIFWADARSRATYEEFGDVVTFDTTYLTNKYDMPFAPFVGVNHHRQSILLGCGLISREDNDSFIWLFRTWLACMSGHAPSGIITDQDKAMKKAIEVVFPNTRHRLCLWHIMKKVPDKLKSYKEYESMSSSLDNIVYDSITSEDFEERGAEMIGKYKFKKQARKWLKSLYAERHHWVPVYVKNKFWAGMSTTQRSESMNAFFDGHVNSKTTLKQFLEQYENALRSKVLKEASADSDSFSSNVRCATHYDMEKQVQAVYTISKFKEFQKELTSIMYCDRVSVETDNAILEYQIAERILIKENKKKTVIFKVQFNEREIEVNCNCCKFEFRGILCRHALYVLVHHDIDFMPDKYIIRRWRKDVKRCHTRVRISYSNWVATPEAQRSDMMQKPLMTLRN