Information report for MDP0000259867
Gene Details
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Functional Annotation
- TrEMBL: A0A498IX10 — A0A498IX10_MALDO; Uncharacterized protein
- STRING: XP_008382761.1 — (Malus domestica)
- GO:0003723 — Molecular Function — RNA binding
- GO:0005509 — Molecular Function — calcium ion binding
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Arabidopsis thaliana: AT3G07490, AT1G05990, AT2G43290
- Juglans regia: WALNUT_00018812-RA
- Nicotiana tabacum: XP_016455343.1, XP_016455342.1, XP_016470842.1, XP_016470835.1
- Petunia inflata: Peinf101Scf01118g05007.1
- Prunus mume: XP_008235883.1
- Prunus persica: Prupe.8G188500.1.p
- Pyrus bretschneideri: Pbr024139.1
Sequences
CDS Sequence:
- >MDP0000259867|Malus_domestica|FAR1|MDP0000259867
ATGGACAAGGCGGAGCTACGGCGGGTTTTCCATATGTTTGATCAGAACGGGGACGGACGGATCACGAAGAAGGAGATGAGGGACTCGTTGGAGAAGCTGGGGATTTACATTCCGGACAAGGACTTGATCCAGATGATCGAAAAAATCGATGTGAATGGAGATGGGTTCGTGGACATGGATGAGTTCGGGGCGTTGTACCAGACGATCATGGACGAGAGGGACGAGGAGGAGGACATGAGGGAGGCGTTCAATGTGTTTGATCAGAACGGAGACGGGTTCATCACGGTGGACGAGCTGCGGTCGGTTTTGTCTTCCTTGGGGCTCAAGCAAGGGAAGACATTGGAGGATTGCACGATCATGATAAAGAAGGTTGATGTGGATGGTGATGGGAGGGTGAATTTCAAGGAGTTTAAGCAGATGATGAAAGGTGGTGGTAGCTTGGGTGATGTTGTATGCAAGATGTTCGACGAAAAGCCTGCTGCTTTTGGGGACTTGGTGGGTACTGCGTGGTTTCCGCCGGAGGGCCGTCGATGGGTTTATTCAGCTCCGTATGTTAACACAGAGCACAAAGCTGTGGAGCCKTTGGAAAATTCGAAAGACGGCGGCGCGGGTGCCGATGGAGCTGGTGATGGTAAAGTGAAAAGGAAAAAGCTCAAGGGGAAAAGGGCAGTGGTTAGGTGGCTCAAGTTCTTCAGGTATAAGAAGAAAAAAGATTATGAGAGAATGACTGCTGAGGAGAAAATTCTCCACAAGTTAAGAAAGGTTGTGGTGAAAACATTCTCACCTGAGGAGGTTAAGGAGATTGCTTCTGAGCTGGCTAGATTAACCGGAGGGATTGTGCTCGGCATTCATGAAGAATACACGATAATTATGTATAGAGGGAAGAACTATTATCAGCCTCCAACAGAAATCATGTCACCTAGGATCACTCTCTCCAGGAAGAAGGCTTTAGATAAATCGAAGTACAAGGATGGTCTTCGTGCCGAAACTGGTACTGAATCTACTGATAATGTAGACTCTGGGAGCTCCTCAAAGTTGAAGCTCGAGGCTTCAAATAAGGTAAAAGAAATGATGGATAGAAGCAAGGAACGCTCTGAGGATGACCTTTCAACAGACGTGGATATATCTTCGGACTCTGAAGATTTATCCGATATTTTCTGTACTGATTCTGACAAGGAGACCGAGAAGAAGGCAGATCGACCTCTGTATTTGGAAGAGTTTGAAAAGTTTACTATTGAAAGTGATGGAGAACCTGAAGATTTTAAGGATCACCTGCGTCAAATATCTAGGGATGCAAAGAAGGATAAATCATTGGACGAAGATGCTGGCTTGCCAAATTTTGATGAGGTTGATCGAATATTTCTGCGTGCTGCTTCACTTTTAAGGAAAAAAACCGGAGCACCTGAAACTTCGGTCGAAAGGGACTTGAATGCAGTTGGAGAACCATATGTTGGTATGGAGTTTGAGTCTGAAGATGCTGCCAAGGAGTTTTACGAAGATTATGCAAGGCGTGTTGGATTCATCATGCGWATAGGCCAGTGTCGTMGATCACATGTTGAGAAGAGAATTCTTTCCCGTARGCTATCCTGCAATAAGCAAGGTGGGCGTTCTTTAAAAGCTAGAGACCAAGTCCGGTCAGTTCGAAGACCGCAACCTACAACAGGAGAAGGGTGTAAAGCGATGATGCTAGTCAAACTTAAAAAATCTGGAAAATGGGTCATTACAAGAGTTGTYAAGGACCATACTCATCCACTTATAGTTTCTTCCGGGAATTCTATGGAAATATGGTTCGACAAAGGATCTGAAAGTGTTTATGGGTACATGATTGCGGAAGTGCATTACTTGCAACCTCTGAATCTGATTTTCTCTCAAATAACTTTGCAGGATGCAAAGGATATGAAGATTGCAGAACTCACTATGGACCTGGAGCGTGAAGAGCAATTATGTGGACAATATCGTGAGCTACTACTTGCATTGCTGAATAATGTTGAGGAGGAGACRGAMCACATAGCAACGAAAGTTAGAGGAGTCGTWAACTATGTAAGAGAATTTGAGACTCAAGTTGAAGGGATTCCAGAAAATCAAATGGAGTCAAGTTCGAGGGCTTCCTCAAAATCAAACGGAGACTGA
Protein Sequence:
- >MDP0000259867|Malus_domestica|FAR1|MDP0000259867
MDKAELRRVFHMFDQNGDGRITKKEMRDSLEKLGIYIPDKDLIQMIEKIDVNGDGFVDMDEFGALYQTIMDERDEEEDMREAFNVFDQNGDGFITVDELRSVLSSLGLKQGKTLEDCTIMIKKVDVDGDGRVNFKEFKQMMKGGGSLGDVVCKMFDEKPAAFGDLVGTAWFPPEGRRWVYSAPYVNTEHKAVEPLENSKDGGAGADGAGDGKVKRKKLKGKRAVVRWLKFFRYKKKKDYERMTAEEKILHKLRKVVVKTFSPEEVKEIASELARLTGGIVLGIHEEYTIIMYRGKNYYQPPTEIMSPRITLSRKKALDKSKYKDGLRAETGTESTDNVDSGSSSKLKLEASNKVKEMMDRSKERSEDDLSTDVDISSDSEDLSDIFCTDSDKETEKKADRPLYLEEFEKFTIESDGEPEDFKDHLRQISRDAKKDKSLDEDAGLPNFDEVDRIFLRAASLLRKKTGAPETSVERDLNAVGEPYVGMEFESEDAAKEFYEDYARRVGFIMRIGQCRXSHVEKRILSRXLSCNKQGGRSLKARDQVRSVRRPQPTTGEGCKAMMLVKLKKSGKWVITRVVKDHTHPLIVSSGNSMEIWFDKGSESVYGYMIAEVHYLQPLNLIFSQITLQDAKDMKIAELTMDLEREEQLCGQYRELLLALLNNVEEETXHIATKVRGVVNYVREFETQVEGIPENQMESSSRASSKSNGD