Information report for KN538871.1_FGP005
Gene Details
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Functional Annotation
- Refseq: XP_015631605.1 — protein FAR1-RELATED SEQUENCE 5 isoform X1
- TrEMBL: Q7Y091 — Q7Y091_ORYSJ; Putative far-red impaired response protein
- STRING: OS03T0655600-01 — (Oryza sativa)
- GO:0009098 — Biological Process — leucine biosynthetic process
- GO:0055114 — Biological Process — oxidation-reduction process
- GO:0009570 — Cellular Component — chloroplast stroma
- GO:0009941 — Cellular Component — chloroplast envelope
- GO:0000287 — Molecular Function — magnesium ion binding
- GO:0003862 — Molecular Function — 3-isopropylmalate dehydrogenase activity
- GO:0008270 — Molecular Function — zinc ion binding
- GO:0051287 — Molecular Function — NAD binding
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Brachypodium distachyon: Bradi1g13310.1.p
- Hordeum vulgare: MLOC_53826.2, MLOC_53826.1
- Oryza sativa: OsIPMDH
- Panicum virgatum: Pavir.9NG179100.1.p, Pavir.9KG084500.1.p, Pavir.9KG084500.2.p, Pavir.9NG179100.2.p, Pavir.9KG084500.3.p
- Pyrus bretschneideri: Pbr015117.1
- Setaria viridis: Sevir.9G150000.1.p, Sevir.9G150000.2.p
- Triticum aestivum: Traes_4DS_01780A949.1
- Ziziphus jujuba: XP_015889514.1, XP_015889513.1, XP_015889512.1
Sequences
CDS Sequence:
- >KN538871.1_FGP005|Oryza_longistaminata|FAR1|KN538871.1_FGP005
ATGTCGCGCCAGTTCGTCTGCGCCAAGGAGGGCTTCCGCACCTACCGCGGGAAGAACGAGGTCTCCCCAGTCGCCGCCGGCAGCGGCGAGGACAGCGGGAGGGGACGCCGGACCCGGGCTGTCACGAGGGTTGGGTGCAAGGCAATGATCCGGGTGAAGAAGCAGGACAATGGCCGGTGGGCGGTCACCAAGCTTGAGACCACGCATAACCATCCTCTGGTCCCTCCCAACCAGGCACACTGCCTGCGCCCTCACAAGCCACTCTCAGAGTGCGGGAAACAGCGTCAATTTGGGATTCCTCGAAATGGTGGTATGCTTCTTGCTATTGAGCCTCCGCCACCACCCATTTCACCGCCCGTGCCGCAGACAAGCGTTCTTCAAGTGGTTCCCCATTATACTAGGGATGGTATTGGAGATCATGCACGACCCGTCCTATTTGGTTGTGCGATCATTGGGGATAACTCGGAGACTTCCTTTGTTTGGTTATTTGAGACACTTCTCTTGGCAATGTCTGGTCACCATCCTGATTCTCTTACCACGGAGCATGATAGTGCCATACAATTGGCTGCCCTTAAGGTCCTTCCCCGAACTAGGCATCGTTTCTGCAGGTGGCACATCTTGAATGAAACACACGATAAGTTATCACACTTATCTGATGAATTTCCGTCCTTACATGAGGAGCTTGTCAATTGTATCAACATGCCTGAGACAATTGATGAGTTTGAAGTAAACTTTAAGGCATTAATTTCTAAGGTTGGCCCAGGAAATAGCGAGTGGCTCTATTCAGTGTATAATTGCCGTCAACATTGGGTCCCCGTGTACTTGAGGGATACATTTTTTGGTGATGAGTCATCAAAGGAGGAGTGTGCAAGCAGGAGTTCATTTTTCGATGGTTATATCAGTGCAAAAACTGATCCACAGTCATTCATCCAGCAGTATGAAAAGGCTCTGGACTGTTGTTATGAGAAGGAGGTGAAGGAAGAATTTGAGACAAAGTACTCACTTCCAGAAATCAAGACATCATCTCCTATTGAGAAACAAGGCGCAGACTTGTACACGAGGTCAATGTTTTTGAAATTCCAACAGGAACTTGTTGATGCCTCTGTTTCCTCTCTGGAAGTAATGAAGGAGGATGGCAAATCTCGCATATATAAAGTGACCAAATCTGCAGGAAGTGAGAAGCCTCATATGGTTGAATTCAATTCTTTTGGAAGTTCTGCAACATGTAGCTGTCAGATGTTTGAACATTTAGGTATTGTCTGTAGGCATATACTTACTATCTTTGGCACACAAGGTGTATCTTCACTTCCTTCCCAGTACATTGTAAAAAGGTGGACTAAATATGCCATGGAAAGAAGTCCAGACAAGAAAATTGATGAAGCTAGCAAAGTCAATGAGCCCAAGGAGGAGCAAAAAAGTGGTGCCGAAGATGGTGAGCAATCTCAGACATGGCGCTACAACAGTTTATGTCGCGAAGCACTTAGATATGCTGAAGAAGGAGCATCATCAGTAGAGGTTTATATTGTAGCAATGCAGGCTCTTCAAGAGGCTGCAAACAAGGTCAATATGGCAAAAAGAGGTATCGGACAGGTAGCACCAAATGCGCCATTGGCAGTGATGCCAATTGCAGCCCAACTACCAGCTGAAGGCTTCAGAAATGTCCAGGAGATTAGTTTTAATCAGAGAAAGAAGAGAAAAAGGAATTCAAACAACAAAACAACAGAGAATTCATCAAATCAACTTATGTATCTACAACAGCCTGTCAATTTTTTGTTTGTTGCTCCTGGTACATCAAGTGGTCCACAAGGGCCTTCTCAAATAGTTGCTGCAGTTCCTGTTTCTTCAAGTGCGCCACACGGTCAGACGACTAGTGCAAACCACCCAATTGATGGAAATACCACATCCTGTTCTGTAGCTGCTCAAAAAAATTCTGACTTGTCCAACTACAGTGCATCAGCACCTTCTTTGGGAAATGTTGTGCCAGAAGGAGAAATTAAATCTTCAGGGTTTGCTTCACAAATAAAAGAGATAAAAATATTGGTGGGGATCATTAATAGGATTAACTATAGTCATGAATTGTCCCAAGGTAATGGCAACAAGGGAAACAACGTGAACATGGCAAGTTCTACATCCTCACCACAGCTTGTGACAGTCCCGGTTGGACTGTGCTTGCCTTCAATGGACAGCTCGAAGATATCTGCAGATGCGATTAATTCTACGAACTCTGGCAGCATGATTAGCAATGGTAATGCGTCATTCGGTCTTTGTCAATCTCAATCTACCAATGCTGATCCTCGGTCAACTCCTGAGGGTTCTTCTATTAGGGCAGCAGCCATCGCAGCAGGTGCACGCATTGCATCTCCATCTGATGCCGCTTCAATCATAAAAGCGGCACAGTCAAAAGGTGCTATCCACATTAGGCCTGGAGAAGGTGTCCCAAATTATCTGAAACCATTAGCCCCACAACCGCTCTCCTCGCTACCACCAGGCAGCATACCCAATTCAGTGCATCCTTCCTCAAGCCATGCACAACCTGGACAGTATAAGTGGGATAACAATGAGAAGCACCTCAAGCCAGAGACTGGGTTGCTCCAGATACGTGCTGGGCTTGGTGTCTTTGCTAATCTGAGGCCAGCTGCAGTGTTGCCTCAGTTAGTAGATGCATCTACTTTGAAAAAAGAAGTAGCTGAAGGAGTCGACATCATGGTTGTTAGAGAGCTTACAGGAGGAATATACTTCGGCCAACCTAGGGGTTTTGGAAAGAACGACAAAGGAGAAGACACTGGCTTCAACACTGAAGTATATTCAGCTTCTGAGATTGATCGAATCGCACGTGTTGCATTTGAGGTTGCTCGCAAGAGACGCGGGAAGCTTTGCTCAGTAGACAAAGCGAATGTGCTTGAGGCATCTATGCTTTGGAGAAAACGGGTGACTTCATTAGCTTCTGAATTCCCTGACATTGAGCTGTCGCACATGTATGTTGATAATGCAGCAATGCAGCTTATTAGGAATCCTAAGCAGTTTGACACAATTGTCACAAACAATATTTTTGGTGACATATTGTCTGATGAAGCATCAATGCTTACTGGAAGCATTGGAATGCTCCCATCTGCCAGTGTTGGTGAATCGGGACCAGGTCTTTTTGAGCCTATTCATGGTTCTGCGCCCGACATTGCTGGGCAGGATAAGGCAAACCCATTAGCTACAATTCTTAGTGCTGCCATGCTATTGCGGTATGGCCTTGGAGAAGAAAATGCTGCAAAGAGAGTTGAAGCCTCAGTTACAGAGACACTAAACAATGGGTTCCGAACTGGGGACATATATTCTCCTGGAACGACCTTGGTCGGATGCAAGCGGATGGGAGAGGAAGTCCTGAAGACTGTAGAGTCGCAGAGCGCGGTTGCTGTAAACTCGTAG
Protein Sequence:
- >KN538871.1_FGP005|Oryza_longistaminata|FAR1|KN538871.1_FGP005
MSRQFVCAKEGFRTYRGKNEVSPVAAGSGEDSGRGRRTRAVTRVGCKAMIRVKKQDNGRWAVTKLETTHNHPLVPPNQAHCLRPHKPLSECGKQRQFGIPRNGGMLLAIEPPPPPISPPVPQTSVLQVVPHYTRDGIGDHARPVLFGCAIIGDNSETSFVWLFETLLLAMSGHHPDSLTTEHDSAIQLAALKVLPRTRHRFCRWHILNETHDKLSHLSDEFPSLHEELVNCINMPETIDEFEVNFKALISKVGPGNSEWLYSVYNCRQHWVPVYLRDTFFGDESSKEECASRSSFFDGYISAKTDPQSFIQQYEKALDCCYEKEVKEEFETKYSLPEIKTSSPIEKQGADLYTRSMFLKFQQELVDASVSSLEVMKEDGKSRIYKVTKSAGSEKPHMVEFNSFGSSATCSCQMFEHLGIVCRHILTIFGTQGVSSLPSQYIVKRWTKYAMERSPDKKIDEASKVNEPKEEQKSGAEDGEQSQTWRYNSLCREALRYAEEGASSVEVYIVAMQALQEAANKVNMAKRGIGQVAPNAPLAVMPIAAQLPAEGFRNVQEISFNQRKKRKRNSNNKTTENSSNQLMYLQQPVNFLFVAPGTSSGPQGPSQIVAAVPVSSSAPHGQTTSANHPIDGNTTSCSVAAQKNSDLSNYSASAPSLGNVVPEGEIKSSGFASQIKEIKILVGIINRINYSHELSQGNGNKGNNVNMASSTSSPQLVTVPVGLCLPSMDSSKISADAINSTNSGSMISNGNASFGLCQSQSTNADPRSTPEGSSIRAAAIAAGARIASPSDAASIIKAAQSKGAIHIRPGEGVPNYLKPLAPQPLSSLPPGSIPNSVHPSSSHAQPGQYKWDNNEKHLKPETGLLQIRAGLGVFANLRPAAVLPQLVDASTLKKEVAEGVDIMVVRELTGGIYFGQPRGFGKNDKGEDTGFNTEVYSASEIDRIARVAFEVARKRRGKLCSVDKANVLEASMLWRKRVTSLASEFPDIELSHMYVDNAAMQLIRNPKQFDTIVTNNIFGDILSDEASMLTGSIGMLPSASVGESGPGLFEPIHGSAPDIAGQDKANPLATILSAAMLLRYGLGEENAAKRVEASVTETLNNGFRTGDIYSPGTTLVGCKRMGEEVLKTVESQSAVAVNS