Information report for Zm00001eb432440
Gene Details
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Functional Annotation
- Refseq: XP_020401724.1 — protein FAR1-RELATED SEQUENCE 6 isoform X1
- TrEMBL: A0A1D6JGA3 — A0A1D6JGA3_MAIZE; Protein FAR1-RELATED SEQUENCE 6
- STRING: GRMZM2G104268_P02 — (Zea mays)
- GO:0055114 — Biological Process — oxidation-reduction process
- GO:0008270 — Molecular Function — zinc ion binding
- GO:0016491 — Molecular Function — oxidoreductase activity
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Brachypodium distachyon: Bradi1g26960.3.p, Bradi1g26960.2.p, Bradi1g26960.1.p
- Hordeum vulgare: MLOC_56322.2, MLOC_56322.1
- Panicum virgatum: Pavir.2NG499200.2.p, Pavir.2NG499200.4.p, Pavir.2NG499200.1.p, Pavir.2NG499200.3.p, Pavir.2KG445600.2.p, Pavir.2KG445600.1.p
- Setaria italica: Seita.2G321500.1.p
- Setaria viridis: Sevir.2G332900.1.p
- Triticum aestivum: Traes_7AL_635989DBF.1, Traes_7BL_C12DC1916.1, Traes_7DL_9D97AB442.1
Sequences
CDS Sequence:
- >Zm00001eb432440|Zea_mays|FAR1|Zm00001eb432440
ATGGCTCCTCTTCAGACTTCAGCTTCTACTACCTCTGTCACTGCTTCATCAGGTGTGACAGCTGAAAGCCTACTTGACCTTTCTCCAGATACAGTCTTCTCCATCCATGCAGATGCAGAAGATGGGTCTCCTCGTAAAGGAGGAGGTTCCTTGGCCCCACCTTTCCCATCTCCATGTACTTTGCGGACTACGCTTCTACGATATGCTGATCTACTCAATCCACCGAAGAAGGCTGCTTTGCTTGCTTTAGCTTCTCATGCATCTGATCCAGCTGAAGCTGAACGACTTAGGTTCTTGGCCTCTCCTGCTGGAAAGGGGGGAAGGTTTGCCTCCTCAATCATGTGGGAGCCTGTGGCGCTGGGTCTGCTCTTTTATCGAACTGTCCTGTTCATGCCGGTGTGGCATACTATGCTTGAGGTGGTGGATGGCTCCCTCCAGAATTCGTGTAACATGTGCATTAGTTTTTGGGCCTTCACCAACAGGAAGGATCCACCAAGGAGTTTGTTCAACATGGATGAAGGGCTGGCTCCTTTTAGAGGTTTCTTGCAGGAAAGGTTAGCACTGAAACAATCTGGAGCAGAACTGGGCACTTCAATCCTTTTCTTTGGATGCAGGAACCGTAATATGGACTACATATATGAAGATGAGCTGCAAACTTTCCTTGAGGCGGGGGCGCTTTCTGAGCTAATTGTTGCATTCTCTCAAGAAGGGCCAACGAAAGAATATGTGCAGCATAAGATGGTGGAGAAGTCTGACCTGAAAATTTTGGAACCAATGGAAGAGGAAGAGTATAGCTCTGAAACATCTAATGAAGAAGATGGCGAAAAAGAAGGAAATGCTGCTGAGAGTGATGTCTTCAGACCAGTTGATGTAGATCCAGCATGTTTACCAAGAGTAGGGATGATATTTGACTCGGAGGAAGATGCCTTTCAGTTTTATGTGACGTATGGTTGTCACGCAGGTTTTGGTATTACAAGAAGATCTAACAATACCTTTGATGGTTTCCGCTATCGCTCTACATTCATATGTTCTAAAGGAGGGCAGTCTAGACTGAGATCTGGTGTGACAAAACCTGCAAGAAAGCGAGGTGTGAAGACTGGCTGCAAGGCCAAGATGATTGTGAAGGATGCCCATTTTCAAAATCGCTGGGAAGTAATTGTTCTTGAGTTGGAGCACAACCATCCATTGGATCCGAACTTGCTTAAGTTTAAGAGACATTTGAAGAACTCCCCTTTTACGATAAATCCACCTCAAATTTGTGAGTCTGAGGGACAACAGAGCAACTCAGCTCTTGTACATTCAAGCAGAGTTGTTGATACTGGTATAACTTCGTCTTCTACACAGATTGAATTCAAAGCCAAGATAGATAGAAATAGGAAATTGAAGCTCGCAGAAGGAGACTTAGACGCCTTGTTGGATTTTCTCAACAAAATGCAAGACCAAAACCCTTGTTTCTTTTACAGTTTAGATATGAATGAGCAAGGGCAACTAAGGAATGTCTTTTGGTCTGACGCCAAATCACGCAGTTCATACAATTACTTTGGTGATGTTGTTGCTATTAATGTCACAAACTTCAGCGATCAGTATGATATGCAGTTTGTGTCATTCATGGGAACTAACCACCATGCACAGCCAGTGTTACTAGGTTGTGGCCTGCTTGCTGGTAGATCTCTTGGGGCTTATGTGTGGCTTTTTGGTACATGGTTAAGATGTATGAATGCCAAACCACCACATTCAATAATTACAAACTACTGTCATGATGTAGCGATAGCTATCAAAAAGGTTTTCCCTAACGCACGTCACCGTTTTTGCCTTTCACACATCTTAAATGTGCTTCCTGAAAAGTTGGAAGAAATTGATAACAAAGATGAAATTATTTCCACATTCACCACACTAGCCTATGATTATGTTACTATGACTGATTTTGACAGAGAGTGGCAAGATACTATCCAACATTTTCGTTTGGAGAGAAATGAATGGCTGTCCAAACTATATGAGGTCAGGATGCAGTGGGCTCCTGTTTATGTTAAGGATTCATTTTGGGCAGGAATGTCTGTCACAGACAGAAGTGATAGTGTAACTGACTATTTTGATGGCTGGTTGATGTCAGGAACCTCTTTGAAAATGTTTGTAGAGCAATATGAGGAAGCTGTTAAAGGAAAGTTAGAAAAGGAATCTTACGAAGATTTACGATCAGCTCAGATGAGGCCACCAATGGTGACGGGGTTGCCTGTGGAAGATCAAGCAGCAAAGGTGTACACTGCAGAAATCTTTCAGAAGTTCTTCAATGAAATAGGACATTCATTTCATTGCAATTATAATATACTTGAACGGAATGAGTCAGTGGTTACATACATAGTTTCGGAGCATGTCGATCAAACAAACAAGGTAGACTACAAAGTTGCTTATGATAATGTTCAAGGTGACATATGGTGCCTCTGCCGTTTGTACCAATCCAAAGGAATATTGTGCAGGCATGCACTCACTGTACTGAGACAGGAGCTTGTGCTGATGATTCCACAAAAATACATCATTCATCGCTGGTGCAAGGACTGTAAACAAACTTGTGCTTCCATATCCCAGCCTGTCTCAGCAGAAGTTGTGGAATTTGGTTCCATGAACTCAGAATCAAAAGAATATGCTTTGTCAATCATGAGGGAAGTCAGGGATAAAGTGATTTCATATGAGAAATCACTAAGAGATCAAAGGGTTGACAGCCATGTTTCAACAGCCAACTTTGCATACAATCCTGTGAATGAGGATTTTACTGATGATGCTTTGCCTATCTCTCTTAGTACAAAGGGCTGGGATCTTACCCAAGGCCAATCTAAACGTTCCCGAAAGAAGAAGCTGGCGACCCCAACTGTTCTTGATACTTTGAAAAAGAAAACTAAAAGGGCATATAACAAAAGGAGGAATGCCACTGCAAACACCCTGAACACATCTGCCACTGCAACTGACGGCATAACTGATGGCACAAATGTGGAGAACATTTCGTTTGATTTGTCACAATACAACAATGCCCCAAGCTTCCACTGGCCTGAAAGCAGCAGCAGGTCTCAGCTCCACTGA
Protein Sequence:
- >Zm00001eb432440|Zea_mays|FAR1|Zm00001eb432440
MAPLQTSASTTSVTASSGVTAESLLDLSPDTVFSIHADAEDGSPRKGGGSLAPPFPSPCTLRTTLLRYADLLNPPKKAALLALASHASDPAEAERLRFLASPAGKGGRFASSIMWEPVALGLLFYRTVLFMPVWHTMLEVVDGSLQNSCNMCISFWAFTNRKDPPRSLFNMDEGLAPFRGFLQERLALKQSGAELGTSILFFGCRNRNMDYIYEDELQTFLEAGALSELIVAFSQEGPTKEYVQHKMVEKSDLKILEPMEEEEYSSETSNEEDGEKEGNAAESDVFRPVDVDPACLPRVGMIFDSEEDAFQFYVTYGCHAGFGITRRSNNTFDGFRYRSTFICSKGGQSRLRSGVTKPARKRGVKTGCKAKMIVKDAHFQNRWEVIVLELEHNHPLDPNLLKFKRHLKNSPFTINPPQICESEGQQSNSALVHSSRVVDTGITSSSTQIEFKAKIDRNRKLKLAEGDLDALLDFLNKMQDQNPCFFYSLDMNEQGQLRNVFWSDAKSRSSYNYFGDVVAINVTNFSDQYDMQFVSFMGTNHHAQPVLLGCGLLAGRSLGAYVWLFGTWLRCMNAKPPHSIITNYCHDVAIAIKKVFPNARHRFCLSHILNVLPEKLEEIDNKDEIISTFTTLAYDYVTMTDFDREWQDTIQHFRLERNEWLSKLYEVRMQWAPVYVKDSFWAGMSVTDRSDSVTDYFDGWLMSGTSLKMFVEQYEEAVKGKLEKESYEDLRSAQMRPPMVTGLPVEDQAAKVYTAEIFQKFFNEIGHSFHCNYNILERNESVVTYIVSEHVDQTNKVDYKVAYDNVQGDIWCLCRLYQSKGILCRHALTVLRQELVLMIPQKYIIHRWCKDCKQTCASISQPVSAEVVEFGSMNSESKEYALSIMREVRDKVISYEKSLRDQRVDSHVSTANFAYNPVNEDFTDDALPISLSTKGWDLTQGQSKRSRKKKLATPTVLDTLKKKTKRAYNKRRNATANTLNTSATATDGITDGTNVENISFDLSQYNNAPSFHWPESSSRSQLH