Information report for EMT16592
Gene Details
Functional Annotation
- TrEMBL: R7W5Z2 — R7W5Z2_AEGTA; Protein FAR-RED ELONGATED HYPOCOTYL 3
- STRING: EMT16592 — (Aegilops tauschii)
- GO:0008270 — Molecular Function — zinc ion binding
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Brachypodium distachyon: Bradi3g29467.1.p, Bradi1g10750.2.p, Bradi5g11315.1.p, Bradi2g55935.1.p, Bradi3g20603.1.p, Bradi1g15474.1.p, Bradi4g13211.1.p, Bradi2g21473.1.p, Bradi3g35045.1.p
- Hordeum vulgare: MLOC_28634.1, MLOC_74313.1
- Triticum aestivum: Traes_2BL_1751C4BBF.2, Traes_5BS_1751C4BBF.2, Traes_1AS_1751C4BBF.2, Traes_6BS_1751C4BBF.2, Traes_1AL_1751C4BBF.2, Traes_4BS_1751C4BBF.2, Traes_7BL_1751C4BBF.2, Traes_2BS_1751C4BBF.2, Traes_6DL_6BFC1BFFF.2, Traes_5AS_4B8974198.2, Traes_7BL_A201C1A091.2, Traes_7BL_A201C1A09.2, Traes_1DL_BE3693D27.2
Sequences
CDS Sequence:
- >EMT16592|Aegilops_tauschii|FAR1|EMT16592
ATGGGAGTGTGTGGCAGCATCCAAAAAAGGAAAAAAATGTTTGAGCTCAATGAGTTGCCTCCTTATCGCAATGAGCTTCCTCATGATACGGATCAGCAGCAACCCAGCATACAACAAGGAAATGGACAGAAAATGGTCGATCTGTTTACTACACGCAGGCAAGTCATGATGTCAGAGAGCCATACTCTAGATGACACGGGAACCGAGGAAAATGTTCCTGGTCCAACCAGGACTGAGCTAGGAGCTGGGGCTGTTGACGGAGCTGTGCAAGGAGAAGAAGGAGAGGATGAGGCTGGTTCTCAACCTATGGAACCCTATGTTGGCATGAGGTTTGACAGCCTTCAAATTGCTAAGGATAACTACAACAGCTACGCACTACGGATGGGTTTCTCTATCAAGATGAACACATCTAGACGGACACCCCGCACCAATGAATTGATAAAACAACAGTTTTGCTGCAACAAGTTCAAGAAGCCCAAAGCTGATGATGGAGGAGCTGAGGCTCCTCCTGTCCTGGACCCTATTCCAGATCCAAAATCTGTTTACAGTGATGAGGAGATGGAAGAAGAACCTCCAATATTTGCTGAAGAGGAGGCTGGTACTAGTAAGAAGAAGAAGAAGCGCAAACGCGAGACAATAAAGCAGACTCAGTGCAAAGCGAAAATGTTGGTGAAGCTGATAGATGGGCGATGGGAGGTGACGCACTTTGTTCGTGACCACAATCATCCGCTCGTGAACAAACCTTCATTGTCCAAATACTTGAGATCCCACCAAGGCATCTCACCTGATGAAAAGGAGTTTCTGCGCATCTTGTATAACTGCAACTTGACTACAGAAGCAAGCTTACAAACCTATACTAGGGACCTTTACCGCAAGTTCAGAGAAGAGTTTGAGCTGATTGGACGTTATAATGCATTCCAAGTTGGTGCTGATATATTTGAGCTCAGACCGAACCAGGAATTTGTTGCCAAATATGGTTCTCGAAACTACTTGGTGCAGGCAAGGGTGGAGGAGGGTTCCTATTTGTGTGAGTGCTGTAAAATGGACAAGGACGACATCCTCTGTTGCCACATCCTCAAGGTGTTCACCCATATTGGAGTTGATGTCAACCGGAAAGGGACTGGTTATGAGCAACCGGATGAAATGCCTCCTCAATCAAAGAAGCAGATAAGGGAGAGGAACATGATATACGATTTTCGGAAACTAGCAAAGTTTGCGAGTGGTTCTGATCCAGCACAAGCTATTGTATACAAGCATATGCGCGCAGCACGTACCGAGATCGGCCACCTGAACATGTCCAAGAAAAAGAAAAAACCAACTGCTGCCACGGGGCCATCAGATGATGGCAACCCTCGAGGACCTCCTCCACCTCCAGGCAACAATCAGGGGGCTCATTATCCAGGTGATCGTGATGGTCCTACTGCCCCGTTGGAGGCTGCATCCCCTGCACAATCTTCTGATGATTTTGTGCCTAGGGATCCTCCAAGGTCTAGTACAAAGGGTAGGGCAAAGAGTCGACGGTACAAATCGGCGCTGGAGCTACACCCAAAGAAGAAAAACAAATGCAGCCAGTGCCAATCTACAGAGCACACTGCTGGTGTGTGTCCACTCAGGCTACTGTGA
Protein Sequence:
- >EMT16592|Aegilops_tauschii|FAR1|EMT16592
MGVCGSIQKRKKMFELNELPPYRNELPHDTDQQQPSIQQGNGQKMVDLFTTRRQVMMSESHTLDDTGTEENVPGPTRTELGAGAVDGAVQGEEGEDEAGSQPMEPYVGMRFDSLQIAKDNYNSYALRMGFSIKMNTSRRTPRTNELIKQQFCCNKFKKPKADDGGAEAPPVLDPIPDPKSVYSDEEMEEEPPIFAEEEAGTSKKKKKRKRETIKQTQCKAKMLVKLIDGRWEVTHFVRDHNHPLVNKPSLSKYLRSHQGISPDEKEFLRILYNCNLTTEASLQTYTRDLYRKFREEFELIGRYNAFQVGADIFELRPNQEFVAKYGSRNYLVQARVEEGSYLCECCKMDKDDILCCHILKVFTHIGVDVNRKGTGYEQPDEMPPQSKKQIRERNMIYDFRKLAKFASGSDPAQAIVYKHMRAARTEIGHLNMSKKKKKPTAATGPSDDGNPRGPPPPPGNNQGAHYPGDRDGPTAPLEAASPAQSSDDFVPRDPPRSSTKGRAKSRRYKSALELHPKKKNKCSQCQSTEHTAGVCPLRLL