Gene Details:

  • Gene ID: EMT13569
  • Gene Family: FAR1 Family
  • Description: FAR1 Family protein
  • Species: Aegilops tauschii
  • Source: FAR1 family gene from PlantTFDB

Protein Features:

Annotation Proteins:

  • Refseq:  XP_020201636.1  — probable LRR receptor-like serine/threonine-protein kinase At1g51820
  • TrEMBL:  R7W7Y8  — R7W7Y8_AEGTA; Putative LRR receptor-like serine/threonine-protein kinase
  • STRING:  EMT13569  — (Aegilops tauschii)

Gene Ontology:

  • GO:0016310  — Biological Process — phosphorylation
  • GO:0005515  — Molecular Function — protein binding
  • GO:0016301  — Molecular Function — kinase activity

Family Introduction:

  • We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
  • We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Literature:

Sequences:

CDS Sequence:
  • >EMT13569|Aegilops_tauschii|FAR1|EMT13569
    ATGGCGGCCCCGTGGTTGCTGCTCCTGCTCTGCGTGGCCGGAGGCGTCCCTCGTGTTCGTTGCCAGAGCGCGCCCGACAGCACTGGTATTGAGCTCCAACATCTCGCAGGCAAAGGCTCAAGCATCATGTCCATGCTCTCAGGTTTCATCAGCATCGACTGCGGCCTCTCGGAGCAGAGCAGCTACGTGGACGATGCCACCGAGCTGGCCTACACCTCGGACGCCGGCTTTATCGACGCCGGCTCCAACTACAACGTCTCGGTCGAGTACATCGACCAGTCCCACTGGAGCCCGCGCAGCCTCCCCCGGCCCCCCGGGGCGGCGCGGCTGCTCCCCCCTCCCGTCCTTCGTGGCGGGGACGTCCAACCCCCCATCTTCGACCTCTACCTCGGCGTCAACTTCTGGAAGACGGTGAACATCTCGAGGCCCGACGCGGTGCACGTCGCGGAGGTCATCGCCGTCGTCCCGGACGACTCCCTGCAGGTCTGCCTGGTGAACACGCGGTCCGGGACGCCCTTCATCTCGTCGCTGGAGCTGAGGCCGCTCAAGGACACGCTGTACCCGCAGGCGAACGCGACGCAGGGGCTGGTGCTCCTCGGCCGGCACAACTTCGGCGCCGCCGATCTGATCAGGTACCCGAACGACACGTACGACCGTGCGTGGACCCCGTACACCAACCCGGAGGACCAGTGGACTACCATCTTGACGTCGTCCAGGGTGGCCATGGAGGTTGAGGATCGCAAGCCGCTCTACGACGTGCCCTCGGTGGTGATGCAGACTGCCGTTCGGCCAAGCAACACCACCAGGAACGTCATGTGGTTCCCCTGGGACGCCGAGCCCAACCACGTCTATCCCATGCCTGGGCTCCTGCCTGTCTTCTATTTGGCGGAGCTGGAGACCGTGGACAGCAAGCAGGAGCGGCGACTACTAGGAGCCGCCAACGCCACGACACTGCGACCGATCATGGAGATCAACTCCACAATACTACCGCCATTCATCAACGCCGCAGAGCTTTTCACCCCTATTTCCACCGCCGGGACCGACGCGCAGGATGTATCTGCCATCACTGCAATCAAGGCTAAGTACCAGTTGATCAAGAACTGGGTGGGTGACCCATGCGCCCCAAAGAACCTAGTGGGGGATGGGCTGAACTGCAGCTATCCCATCTCCAGACCTCAGAGAATCACAAGCGTAAACATGTCATTTGGCGGTTTGAGTGGTGATATATCATCATACTTTGCCAATCTCAAAGCTATCCAGTACCTGGATTTGTCACATAATAACTTGACTGGATCAATTCCTGATGGTCTTTCACAACTACCCTCTCTCGTGCTACTCAACGTCACGCCGTCATCACGTTCGTCATCGGAGAAAATGGATCCGGCTGAAATCCTCGCCGCCGTGCAAGAGTTAGAGGAGGAGGGCGATTATGATGGCGATGATTACGGCGAGGACAAAGAAACCTCGTCACTGAATATGGATGAACCTGCTGAGGAGAACTACATAAGTTTGGATGAATCTTATAATGGCAATCGACATGGCGATGATGATGATGATATGGATATAGATGATTCATTTGCTGAAAGTGCACTCCGGATGGACGAGGACGGGGACTTTGTGAGGAATATTGTGGACGATGAAAGTGACAAATCGCACGTTGATGCATCTCATGATGACAGCTCCAGCAAAGGAGATGTAGATGCTACAAGCGGGAAGGATGAGCATGTTGGTGATGATCTGTTTAATTTCGACATCGGATTTGATGAATATCAGCAAGAATCATTGGACATGCATTGGAAGGTCATGAGGAAGACGTTCAAGTCACTAGGAGAGGCTTACAATTTCTATAACCAGTATGCTTACGAGCGTGGTTTCAGCGTCAGAAAGGACTCACTGAAATATTCGAAAGGTCCTGAGGGAACTAAGCGCTTGAGAAAGTATCTGTGTGCGAGAGCTGGGAAACGAGAGGCAAAACTTTGTACAATGGAAGGCAGGACCCGCAGGCTGAGAGGGGAGACTCGTTGCTTCTGCGATGCGCATATAACTTTGAAATTTGATAAAGAGCGTGACGTTTGGTATGTCAGCAGTTTCAGTGATGATCACAGTCACGTTCTAGCTAGACCGGATGAAGTCACGTTTCTTCGGTCTCATAATCAGATAAAAGAATATCAGAAAGCTGAGATCTTAACTATGGCAGGTGCTGGAATCAGGAAACATATGATTTATGATCAACTCGTCAGTAGGTACGGGTCATATGCAAAGGCTGGTTTTGGCAGGAAGAAGCTTTATAACATGTGTTATAGAGAAAAAATGAAGTTGCTTGCACAAGGTGATGCAGACACTGCCATTGGGATCATGATGACCAGAAGAGAGAGGGATCCAGATTTCTTCTTTGAGCACACCGTCGATGCTGGAGGAAGGTGCCGTCGGTCAAGTCTGACGTCAGACTGGCCGGCGCACTTGTCGGGCTATTTTTTTAAAAGAAAAACTAAGAACGTCGGTGTTACAAGCGACGGCGGCAGTGCAGAAAAACAACAGTAA
Protein Sequence:
  • >EMT13569|Aegilops_tauschii|FAR1|EMT13569
    MAAPWLLLLLCVAGGVPRVRCQSAPDSTGIELQHLAGKGSSIMSMLSGFISIDCGLSEQSSYVDDATELAYTSDAGFIDAGSNYNVSVEYIDQSHWSPRSLPRPPGAARLLPPPVLRGGDVQPPIFDLYLGVNFWKTVNISRPDAVHVAEVIAVVPDDSLQVCLVNTRSGTPFISSLELRPLKDTLYPQANATQGLVLLGRHNFGAADLIRYPNDTYDRAWTPYTNPEDQWTTILTSSRVAMEVEDRKPLYDVPSVVMQTAVRPSNTTRNVMWFPWDAEPNHVYPMPGLLPVFYLAELETVDSKQERRLLGAANATTLRPIMEINSTILPPFINAAELFTPISTAGTDAQDVSAITAIKAKYQLIKNWVGDPCAPKNLVGDGLNCSYPISRPQRITSVNMSFGGLSGDISSYFANLKAIQYLDLSHNNLTGSIPDGLSQLPSLVLLNVTPSSRSSSEKMDPAEILAAVQELEEEGDYDGDDYGEDKETSSLNMDEPAEENYISLDESYNGNRHGDDDDDMDIDDSFAESALRMDEDGDFVRNIVDDESDKSHVDASHDDSSSKGDVDATSGKDEHVGDDLFNFDIGFDEYQQESLDMHWKVMRKTFKSLGEAYNFYNQYAYERGFSVRKDSLKYSKGPEGTKRLRKYLCARAGKREAKLCTMEGRTRRLRGETRCFCDAHITLKFDKERDVWYVSSFSDDHSHVLARPDEVTFLRSHNQIKEYQKAEILTMAGAGIRKHMIYDQLVSRYGSYAKAGFGRKKLYNMCYREKMKLLAQGDADTAIGIMMTRRERDPDFFFEHTVDAGGRCRRSSLTSDWPAHLSGYFFKRKTKNVGVTSDGGSAEKQQ