Information report for AHYPO_017318-RA
Gene Details
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Functional Annotation
- Refseq: XP_027368884.1 — putative protein FAR1-RELATED SEQUENCE 10 isoform X4
- Swissprot: Q9LKR4 — FRS10_ARATH; Putative protein FAR1-RELATED SEQUENCE 10
- TrEMBL: A0A0B0Q0U7 — A0A0B0Q0U7_GOSAR; Far1-related sequence 10-like protein
- STRING: EOY28958 — (Theobroma cacao)
- GO:0008270 — Molecular Function — zinc ion binding
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Actinidia chinensis: Achn014121
- Cucumis sativus: Cucsa.100320.1
- Gossypium arboreum: Cotton_A_18282_BGI-A2_v1.0
- Manihot esculenta: Manes.07G106900.8.p, Manes.07G106900.7.p, Manes.07G106900.6.p, Manes.07G106900.5.p, Manes.07G106900.4.p, Manes.07G106900.3.p
- Prunus mume: XP_016648403.1, XP_008227430.1
- Prunus persica: Prupe.4G006000.6.p, Prupe.4G006000.1.p
Sequences
CDS Sequence:
- >AHYPO_017318-RA|Amaranthus_hypochondriacus|FAR1|AHYPO_017318-RA
ATGTCTGGTAGGAATAATTTATGGCTGAGAAAACAGAACTGCCCATGTGGAGATTGGAAATGTTATATTACTTATGATGCACAAACTGATGAGACATCATTTACATCTCAAGTAAGCATTAAGAATGAAACAAACCAATTATTTGAGGACATGATTGACCCTTATGTTGGAATGGGGTTTAAAACTGATGTTGATGCTTTTGATTATTATGGGAGTTTTGCTAGGAAGAATGGGTTTTCGATTAGGAAAGAACGGTCGAGGATAAGCCCTCAATTGGGCATTTATAAGAGAGATTTTGTTTGTTATCGTTCTGGTTTTGCATCTTCTAGGAGGAAAGCCTCTAGTGAGCACCAGAGGGATAGGAAGTCTGTGCGATGTGGTTGTGATGCGAAAATGTTCTTGTCTAAGGAAGTTGTTGATGGGGTTTCTCAGTGGTTTGTTGTTCAGTTTAGCAATGTTCATAACCATGAGTTGTTAGAAGATGATCAAGTTCGTCTTCTTCCTGCTTATCGTAAAATTCAGGAGCCAGATCAAGAGCGCATCCTTTTGCTCTCGAAGGCTGGATTTCCTGTACATCGTATAGTGAAAGTCTTGGAGTTGGAAAAAGGAATTAATGGAGGGCAGCTGCCTTTTCTAGAGAGAGATGTTAGGAACTTTGTTCAGAATCGGAAAAGAATGGCTCAAGAAAGTGATGCTTTACTTTCTGAGAAGCGAGATAGTGAAATATTGGAACTTTTAGAGGCTTATACTGGGCTTCAAGAGTCAATAAGGAGTGAATTACCTAGTACAAAACACATCATTTCTGTATGGTATATTCTGCCTAAGTTATCCGGTTGGTTCTCTCTTCTTCTTGGGTCAAGTTTTGCTGACTTTAGGTCTGAGTTTGATGTGCTATACCGCATGGAAAGTACTGAGCAGTTTGAGTTTGAATGGACTCAGATGGTTGCTCGATTTGAACTGGAGTCCGAGAAACATTCTAGTTTATTATTTTCATTGAGAGAATCCTGGGCATTATCTTATGTTCGAGGCTATCTTCTTGCTCAGATGGCAACATTAGCATATTCAAAATGCGTAGATATGTTCTTAAAAGGCATAGTCAACAAACAACCCTGTTTATGCAGCTTTTTTGAACGGATCAGCCACGCTAGAAGTATACAAAGGCAGGCATACCCCGATATGCGAGGTATACAAACGAGGACATGCGTGCCTATTGAAGAGCATGCTCGAAATATCCTCACTCCCTTTGCTTTCGGTGTGTTTAAGCAAGAGTTGATGCTTGCTATGCAGTATGCAGTTTCTGAGATGGCTAATGGTTCTTGTCTTGTTCAACACTTCAAGAAAATGGATGGGGAGCGCTTTGTAATATGGATACCAGAAGGTGAACAAATCCATTGTTCCTGTAAGGAGTTTGAATCTTCTGGAATTCTATGTAGGCATGCTCTTCGAGTATTCATAGCAAAGAACTACTTTGAACTGCCTGAAGTATACTTTTCTAGAAGATGGCAGAAAATTCAATATGATGACCAACCGGTTCAAATAAGCAAAGATGAGTGGTTTCAAGAATTTCATGATCTCACGTCAACTCTATTTACCGAATCATTGGTAACAAAAGAAAGATTTGATTTGGTACAAAAAGAACTTAGTAAAGAGCTTCTAAGACTTATTAATGAGGTTAGAAGTATGCCTGCGAATGATGAAGTTGGATAA
Protein Sequence:
- >AHYPO_017318-RA|Amaranthus_hypochondriacus|FAR1|AHYPO_017318-RA
MSGRNNLWLRKQNCPCGDWKCYITYDAQTDETSFTSQVSIKNETNQLFEDMIDPYVGMGFKTDVDAFDYYGSFARKNGFSIRKERSRISPQLGIYKRDFVCYRSGFASSRRKASSEHQRDRKSVRCGCDAKMFLSKEVVDGVSQWFVVQFSNVHNHELLEDDQVRLLPAYRKIQEPDQERILLLSKAGFPVHRIVKVLELEKGINGGQLPFLERDVRNFVQNRKRMAQESDALLSEKRDSEILELLEAYTGLQESIRSELPSTKHIISVWYILPKLSGWFSLLLGSSFADFRSEFDVLYRMESTEQFEFEWTQMVARFELESEKHSSLLFSLRESWALSYVRGYLLAQMATLAYSKCVDMFLKGIVNKQPCLCSFFERISHARSIQRQAYPDMRGIQTRTCVPIEEHARNILTPFAFGVFKQELMLAMQYAVSEMANGSCLVQHFKKMDGERFVIWIPEGEQIHCSCKEFESSGILCRHALRVFIAKNYFELPEVYFSRRWQKIQYDDQPVQISKDEWFQEFHDLTSTLFTESLVTKERFDLVQKELSKELLRLINEVRSMPANDEVG*