Gene Details:

• Gene ID: AA87G00209
• Gene Family: FAR1 Family
• Description: FAR1 Family protein
• Species: Aethionema arabicum
• Source: FAR1 family gene from PlantTFDB

Protein Features:

• Pfam:  PF03101
• Pfam:  PF10551
• InterPro:  IPR004330    IPR018289

Annotation Proteins:

• Refseq:  XP_006414566.1  — protein FAR-RED IMPAIRED RESPONSE 1
• Swissprot:  Q9SWG3  — FAR1_ARATH; Protein FAR-RED IMPAIRED RESPONSE 1
• TrEMBL:  A0A1J3JL92  — A0A1J3JL92_NOCCA; Protein FAR-RED IMPAIRED RESPONSE 1
• STRING:  XP_006414566.1  — (Eutrema salsugineum)

Gene Ontology:

• GO:0010018  — Biological Process — far-red light signaling pathway
• GO:0042753  — Biological Process — positive regulation of circadian rhythm
• GO:0045893  — Biological Process — positive regulation of transcription, DNA-templated
• GO:0005634  — Cellular Component — nucleus
• GO:0003700  — Molecular Function — transcription factor activity, sequence-specific DNA binding
• GO:0008270  — Molecular Function — zinc ion binding

Family Introduction:

• We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
• We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.

Literature:

• Transposase-derived transcription factors regulate light signaling in Arabidopsis.   DOI:  10.1126/science.1146281 ;  PMID:  18033885

Sequences: