Information report for 676720782
Gene Details
Functional Annotation
- GO:0005509 — Molecular Function — calcium ion binding
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Arabidopsis thaliana: AT3G07500.1, AT3G07500, AT3G07490
- Brassica napus: GSBRNA2T00059491001, GSBRNA2T00038984001
- Brassica oleracea: XP_013583896.1
- Brassica rapa: XP_009147067.1
- Manihot esculenta: Manes.13G055700.2.p
- Populus trichocarpa: Potri.014G176500.1
- Prunus mume: XP_016650635.1
- Prunus persica: Prupe.8G188300.1.p
- Raphanus raphanistrum: RrC792_p4
- Sesamum indicum: XP_011089606.1
- Vitis vinifera: GSVIVT01001896001
Sequences
CDS Sequence:
- >676720782|Sisymbrium_irio|FAR1|676720782
ATGGATCAAGCAGAGCTTTGCCGGATATTCCAAATGTTCGACAGGAACGGCGACGGGAAAATCACCAAGCAAGAGCTGAATGATTCATTGGAGAATCTTGGAATCTACATCCCCGACAAAGACTTGGTGCAGATGATTGAGAAAATTGATCTCAACGGTGATGGGTACGTGGACATCGAAGAGTTTGGAGGGTTGTACCAGACGATCATGGAGGAGAGAGACGAGGAAGAAGACATGAGAGAGGCTTTTAATGTGTTTGATCAGAACCGGGACGGGTTCATCACGGTAGAAGAGCTGAGATCAGTGTTAGCTTCCTTGGGGCTCAAGCAAGGAAGAACCCTAGAGGATTGCAAGAGGATGATAAGCAAAGTGGACGTTGATGGAGATGGGATGGTAAATTTCAAGGAGTTTAAACAAATGATGAAAGGTGGTGGATTTGCCGCCTTAGGATCAAACTTAAAATCAAACAGTGTATGGTATGAACATGGTGGTAAAATCCAACGGATGAAGTTCACGTGCCTCCACCCAAAAGCAAGTCAGTCATCATCAACCGTACAAGAACATAATGAAGAGAGATCTTCGACTTGGTTTTTGTTGTCGTCTTCCTCACCACTTCCCTTTCTTTGCCACTCACTAAGTTCAGTTTCAGAGATCATTCATCATCACTGCCTCTCTCGAAGTCTCCCAATTTGGAGTTTCCTAATCTTCCATCGAATGGAAGCACTGGAAGGTAACCCTTTACGTGGAGAAGGTGTAGAGATAATGGAGAATTCAAATGGAAAGTATACGGTCACAGATGAAGCTTCTTCAATGGTGGAGCCCTTCATTGGTATGGAGTTTGACTCCGAAGAAGCTGCAAAGTCTGTCTATGACAGTTACGCTACGAGCACGGGTTTCGTCATGAGAGTGGATGCTTTCAGGCGGTCAATGCGAGATGGGACCGTGGTGTGGCGCAGGCTCGTGTGTAATAAAGAAGGGTTCCGTAGATCGAGACCTCGTAGAAGCGAGAGCAGGAAGCCTCGAGCTATAACAAGAGAAGGGTGTAAGGCTTTGATTGTGGTCAAGAAAGAGAAATCCGGAAAATGGCTTGTCACTAAATTTATAAAAGAACATAATCATCCTCTCTTGCCTCGCCTGCTCCTGCCTAATGCTCGCCGCAGTTCACAGCTCTCTCAAACACCGGACGAGAAAGACGCAAAGATTAAGGAGCTTAACACAGAGCTGAGCCGAGAGAGAAGGCGTTGTGCTGTGTTGCAACAGCAGCTTGATATGGCCCTAAAGTTGATGGAAGAACACTCTAATCATCTCTCCATTAAAATCAGTAATGTTATCCAAAATGTAAGAGAAATAGAGTCCAATACTTTCACCAAACCCCTCAGCTGA
Protein Sequence:
- >676720782|Sisymbrium_irio|FAR1|676720782
MDQAELCRIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEEEDMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEFKQMMKGGGFAALGSNLKSNSVWYEHGGKIQRMKFTCLHPKASQSSSTVQEHNEERSSTWFLLSSSSPLPFLCHSLSSVSEIIHHHCLSRSLPIWSFLIFHRMEALEGNPLRGEGVEIMENSNGKYTVTDEASSMVEPFIGMEFDSEEAAKSVYDSYATSTGFVMRVDAFRRSMRDGTVVWRRLVCNKEGFRRSRPRRSESRKPRAITREGCKALIVVKKEKSGKWLVTKFIKEHNHPLLPRLLLPNARRSSQLSQTPDEKDAKIKELNTELSRERRRCAVLQQQLDMALKLMEEHSNHLSIKISNVIQNVREIESNTFTKPLS