Information report for 462922416
Gene Details
Functional Annotation
- Refseq: XP_004960758.1 — la-related protein 6B
- TrEMBL: K3Z5P0 — K3Z5P0_SETIT; Uncharacterized protein
- STRING: Pavir.Cb01883.1.p — (Panicum virgatum)
- GO:0006396 — Biological Process — RNA processing
- GO:0005634 — Cellular Component — nucleus
- GO:0030529 — Cellular Component — intracellular ribonucleoprotein complex
- GO:0000166 — Molecular Function — nucleotide binding
- GO:0003723 — Molecular Function — RNA binding
Family Introduction
- We show that Arabidopsis FHY3 and FAR1, which encode two proteins related to Mutator-like transposases, act together to modulate phyA signaling by directly activating the transcription of FHY1 and FHL, whose products are essential for light-induced phyA nuclear accumulation and subsequent light responses. FHY3 and FAR1 have separable DNA binding and transcriptional activation domains that are highly conserved in Mutator-like transposases. Further, expression of FHY3 and FAR1 is negatively regulated by phyA signaling. We propose that FHY3 and FAR1 represent transcription factors that have been co-opted from an ancient Mutator-like transposase(s) to modulate phyA-signaling homeostasis in higher plants.
- We next used a yeast one-hybrid assay to delineate the DNA sequences to which FHY3 and FAR1 bind. GAD-FHY3 or GAD-FAR1 fusion proteins (GAD, GAL4 transcriptional activation domain), but not GAD alone, activated the LacZ reporter genes driven by the FHY1 and FHL promoters. Deletion analysis narrowed down the FHY3/FAR1 binding site to a 39-bp promoter subfragment located on the ‘a’ fragment for both FHY1 and FHL. Notably, these subfragments share a stretch of consensus sequence, 5’-TTCACGCGCC-3’. Mutating the core sequence ‘CACGCGC’ of this motif (m2 and m3 for FHY1, m5 for FHL) abolished the reporter gene activation by both GAD-FHY3 and GAD-FAR1. Mutating the flanking sequences (m1 and m4) did not obviously affect the reporter gene activation by GAD-FAR1, but clearly reduced activation by GAD-FHY3. Thus, ‘CACGCGC’ likely defines a cis-element that confers specific binding for FHY3 and FAR1 and is named FBS for FHY3-FAR1 binding site.
Literature and News
Homologs
- Arabidopsis thaliana: AT2G43970
- Brachypodium distachyon: Bradi4g40020.1.p
- Hordeum vulgare: MLOC_38921.4, MLOC_38921.2, MLOC_38921.3, MLOC_38921.7
- Musa acuminata: Ma04_g30240
- Panicum virgatum: Pavir.3KG066300.1.p, Pavir.3NG061100.1.p
- Setaria italica: Seita.3G094700.1.p
- Setaria viridis: Sevir.3G095000.1.p
- Triticum aestivum: Traes_5DS_61EE28B2A.2, Traes_5AS_1986BD81F.2
Sequences
CDS Sequence:
- >462922416|Eragrostis_tef|FAR1|462922416
ATGTCCCTGGACACCAATGCTCGATCCGGTCAGAATGATAGTCTAGTTCTTGGAGATTCTGTTGTGGAAGAGGCGCCTAATTTCTCTGCTGAAGCTGCAGCAGGGCCAGATGTTATGAAAGATTTTGCTCCAGCTGAGGATCCAAATGAACCGGCCCTCGGTATGACATTTGAATCTGATGAGGCTGCTAAGGAGTTCTATGACGAGTATGCCAGGCGGCTTGGTTTCCCCTTCCGTGTCGGCAGGTCTCGGCGCTCCAAGGGTACAGAGGAGGTTCTTGTCATGAAGAGGTTTGTTTGCTCCAGGGAAGGGATGTACAAGAAGAAGAAGCTGGCGTCTGATGAGGCGACAAGGAAGCGCGAGAGGATGTCAATGCGGGAAGGTTGCAATGCTATGATGGAGGTGGTTAGGGACTCCGATCACTGGGCTGTCTCCAAGCTTGAGAAGGCTCATAACCATGACCTGGGCGCTTGTAGCAAGGTTGGGTACCTTCGCGCAAGAGGCGCGCAGGCGCAGCGAGCCGGCGAGGTCGGCGGCGCCGCGCAGGACGGCGCCGCGGGAGGCGGTGTCCACGACGACGGAGAAGTTGCGCGAGATGAGCGCGACATCGGGCGTCAGGGCCACCCCGCCTCCCCCGGCGCCGTGGCTGACGCGGAGGCGGAGCTTGGCGAGCCCGTCGAGGACGCGGCAGGAGAAGCGGTGGGAGAAGAGCCGCCGCTCGCTGTCGAACTCGGAGCTCTCCCCTGTACCCCCGCCGCCGCCGCCGCTGCTGCTAGGGAGCTTCCAGTCCCCCGNNNNNNNNNNNNNNAGTCGGGCCCGCGGGCCCCGCCGCAGCCGCCGCCGGTGGCCGTGGTGCCGCCGCCCCCCGTGATCCGCGTGTTCGCCGCGCCTCCACCGCCGCCGCGCGCGGCCTTCTTCGCGGCGCCTCCCCCGCCCCCGCAGTTCGAGTACTACGCGCCCGTCGGGGGACGCGGTGGATTCGCCGCCAAGGAGCCGAAGCCTGTGCCGCAGCCGGAGCCGGAGGTGGAGCAGCCGCCGCCGGCTGCAGGGGGGGAGCCGCTGGTGGAAGGGCTCGCCGACGAGGTGGTTCACAAGATTACCAAGCAGGTGGAGTACTATTTCAGTGACATAAACCTGGCTACCACAGAACATTTGATGAGATTCATTACCAAGGATTCTGAAGGATATGTGCCAATATCTGTTATCGCTGGGTTCAAGAAAGTAAAAACTTTGGTGCATAATAACTCGATGCTTGCTGCTGTTCTCCGAACCTCGTCAAAACTCATTGTAAGCGAGGATGGGAAAAGAGTCAAACGTCAGGAGCCATTTACTGAATCTGATTTGCAGGCGCTCCAGTCCCGAATTATTGTTGCAGAAAACCTTCCTGGTGATCCTTCATACCAGAATCTTAAGAAGATATTTTCTTCTGTTGGGAGTGTGATTTCAATCCGTACTTGCTATCCTCAGACTCCGAATGGTACTGGTCCTGCTACTAATAGATCTGCCAAGCTAGATATGCTTTTTGCTAACAAGCTTCATGCTTTTGTGGAGTACGAGACTCCCGAGGATGCTGAGAAGGCTACAAAGGGGGCAGGCAAAGGCAAAAAGGGTGTGCCTGAAACTGATGAAAATGGCGAGGACGTCTCCACGTCGAACCAATCAACTGAGAAACAGTTTGAAGAATCATCTCAACTGTTAGATGTATTGCCAGAACACTTGTTTGATGATAACTTCACTGAGAAGGAAGTTCCAAGACGTGGCAAAGGACGTGGACGCGGTGGCAGAGGCCGTGGGCGTGGTTATCAGCAGTATAACAACAATCAGTACCACCAGAACCATCAGCACTACAATCACCACAGTGGCAACAACCATGGTAGCAACCGCGGTGGTGGCCACAACCATCATGTCGGGACTCCGCCAAATAATCAGCCAATCAAGCCACAACCTCCAGGCCCGCGCATGCCTGATGGCACGCGAGGTTTCGCGATGGGCAGAGGGAAACCAGAAGTCATGCTTCCAGGTTTGTGCGCTGTTGGTGAGCCATGA
Protein Sequence:
- >462922416|Eragrostis_tef|FAR1|462922416
MSLDTNARSGQNDSLVLGDSVVEEAPNFSAEAAAGPDVMKDFAPAEDPNEPALGMTFESDEAAKEFYDEYARRLGFPFRVGRSRRSKGTEEVLVMKRFVCSREGMYKKKKLASDEATRKRERMSMREGCNAMMEVVRDSDHWAVSKLEKAHNHDLGACSKVGYLRARGAQAQRAGEVGGAAQDGAAGGGVHDDGEVARDERDIGRQGHPASPGAVADAEAELGEPVEDAAGEAVGEEPPLAVELGALPCTPAAAAAAARELPVPXXXXXXSGPRAPPQPPPVAVVPPPPVIRVFAAPPPPPRAAFFAAPPPPPQFEYYAPVGGRGGFAAKEPKPVPQPEPEVEQPPPAAGGEPLVEGLADEVVHKITKQVEYYFSDINLATTEHLMRFITKDSEGYVPISVIAGFKKVKTLVHNNSMLAAVLRTSSKLIVSEDGKRVKRQEPFTESDLQALQSRIIVAENLPGDPSYQNLKKIFSSVGSVISIRTCYPQTPNGTGPATNRSAKLDMLFANKLHAFVEYETPEDAEKATKGAGKGKKGVPETDENGEDVSTSNQSTEKQFEESSQLLDVLPEHLFDDNFTEKEVPRRGKGRGRGGRGRGRGYQQYNNNQYHQNHQHYNHHSGNNHGSNRGGGHNHHVGTPPNNQPIKPQPPGPRMPDGTRGFAMGRGKPEVMLPGLCAVGEP