Gene Details:

• Gene ID: Pbr010447.1
• Gene Family: EIL Family
• Description: EIL Family protein
• Species: Pyrus bretschneideri
• Source: EIL family gene from PlantTFDB

Protein Features:

• Pfam:  PF04873
• Gene3D:  G3DSA:1.10.3180.10
• SuperFamily:  SSF116768
• InterPro:  IPR023278

Annotation Proteins:

• Refseq:  XP_009339247.1  — PREDICTED: calcium/calmodulin-dependent serine/threonine-protein kinase
• Refseq:  XP_009339248.1  — PREDICTED: calcium/calmodulin-dependent serine/threonine-protein kinase
• Swissprot:  Q6RET7  — CCAMK_MEDTR; Calcium and calcium/calmodulin-dependent serine/threonine-protein kinase DMI-3
• TrEMBL:  A0A498JH27  — A0A498JH27_MALDO; Uncharacterized protein
• STRING:  XP_009339247.1  — (Pyrus x bretschneideri)
• STRING:  XP_009339248.1  — (Pyrus x bretschneideri)

Gene Ontology:

• GO:0009608  — Biological Process — response to symbiont
• GO:0009877  — Biological Process — nodulation
• GO:0046777  — Biological Process — protein autophosphorylation
• GO:0004683  — Molecular Function — calmodulin-dependent protein kinase activity
• GO:0005509  — Molecular Function — calcium ion binding
• GO:0005524  — Molecular Function — ATP binding
• GO:0009931  — Molecular Function — calcium-dependent protein serine/threonine kinase activity

Family Introduction:

• The gaseous phytohormone ethylene regulates various processes related to growth, development, and stress response of higher plants. A largely linear pathway of ethylene signaling was revealed by a number of molecular genetic studies. In the pathway, Ethylene-insensitive3 (EIN3) and EIN3-like (EIL) proteins are the key transcription factors. The EIN3/EIL proteins were shown to regulate expression of the GCC-box-binding transcription factors, ethylene-response factors. It is known that the phenotype of the Arabidopsis thaliana EIN3 (AtEIN3)-deficient mutants, e.g. long seedlings in the presence of ethylene or its precursor, is suppressed by overexpression of Arabidopsis EIL1 (AtEIL1) or EIL2 (AtEIL2), indicating the functional equivalence of these proteins.
• In the present study, a region corresponding to the major DBD of the EIN3/EIL transcription factor family, which is the key factor in the ethylene signaling, was identified. The isolated domain exists in a monomeric form and retained the sequence specificity for the DNA binding. The structure determined by NMR spectroscopy consists of five alpha-helices, packing into a novel fold dissimilar to known DBDs. An NMR titration analysis suggested that a region including the key mutation site, ein3-3 site, was shown to be involved in DNA binding.

Literature:

• Solution structure of the major DNA-binding domain of Arabidopsis thaliana ethylene-insensitive3-like3.   DOI:  10.1016/j.jmb.2005.02.065 ;  PMID:  15811366

Sequences: