Information report for EMT14735
Gene Details
Functional Annotation
- Refseq: XP_020187998.1 — ETHYLENE INSENSITIVE 3-like 3 protein
- Swissprot: O23116 — EIL3_ARATH; ETHYLENE INSENSITIVE 3-like 3 protein
- TrEMBL: A0A3B6MUW5 — A0A3B6MUW5_WHEAT; Uncharacterized protein
- TrEMBL: A0A453L4Q1 — A0A453L4Q1_AEGTS; Uncharacterized protein
- TrEMBL: N1QWS5 — N1QWS5_AEGTA; ETHYLENE INSENSITIVE 3-like 3 protein
- STRING: EMT14735 — (Aegilops tauschii)
- GO:0000165 — Biological Process — MAPK cascade
- GO:0006355 — Biological Process — regulation of transcription, DNA-templated
- GO:0006612 — Biological Process — protein targeting to membrane
- GO:0009409 — Biological Process — response to cold
- GO:0009414 — Biological Process — response to water deprivation
- GO:0009723 — Biological Process — response to ethylene
- GO:0009733 — Biological Process — response to auxin
- GO:0009738 — Biological Process — abscisic acid-activated signaling pathway
- GO:0009743 — Biological Process — response to carbohydrate
- GO:0009867 — Biological Process — jasmonic acid mediated signaling pathway
- GO:0010363 — Biological Process — regulation of plant-type hypersensitive response
- GO:0031348 — Biological Process — negative regulation of defense response
- GO:0042538 — Biological Process — hyperosmotic salinity response
- GO:0042762 — Biological Process — regulation of sulfur metabolic process
- GO:0043069 — Biological Process — negative regulation of programmed cell death
- GO:0050832 — Biological Process — defense response to fungus
- GO:0071281 — Biological Process — cellular response to iron ion
- GO:0005634 — Cellular Component — nucleus
- GO:0003677 — Molecular Function — DNA binding
- GO:0003700 — Molecular Function — transcription factor activity, sequence-specific DNA binding
Family Introduction
- The gaseous phytohormone ethylene regulates various processes related to growth, development, and stress response of higher plants. A largely linear pathway of ethylene signaling was revealed by a number of molecular genetic studies. In the pathway, Ethylene-insensitive3 (EIN3) and EIN3-like (EIL) proteins are the key transcription factors. The EIN3/EIL proteins were shown to regulate expression of the GCC-box-binding transcription factors, ethylene-response factors. It is known that the phenotype of the Arabidopsis thaliana EIN3 (AtEIN3)-deficient mutants, e.g. long seedlings in the presence of ethylene or its precursor, is suppressed by overexpression of Arabidopsis EIL1 (AtEIL1) or EIL2 (AtEIL2), indicating the functional equivalence of these proteins.
- In the present study, a region corresponding to the major DBD of the EIN3/EIL transcription factor family, which is the key factor in the ethylene signaling, was identified. The isolated domain exists in a monomeric form and retained the sequence specificity for the DNA binding. The structure determined by NMR spectroscopy consists of five alpha-helices, packing into a novel fold dissimilar to known DBDs. An NMR titration analysis suggested that a region including the key mutation site, ein3-3 site, was shown to be involved in DNA binding.
Literature and News
Gene Resources
- Pfam: PF04873
- Gene3D: G3DSA:1.10.3180.10
- SuperFamily: SSF116768
- InterPro: IPR023278
Sequences
CDS Sequence:
- >EMT14735|Aegilops_tauschii|EIL|EMT14735
ATGGATCAGCTGGCTCTGCTCGCGACGGAGTTCGGAGATTCGTCCGACTTCGAGGTCGACGGCATCAACGAGAACGATGTCAGCGACGAGGAGATTGAGCCGGAGGAGCTGGCCCGGCGAATGTGGAAGGACAGGGTCAGGCTCAGGAGGATCAAGGAGAGGCAGCAGAGGTTAGCCTTGCAGCAGGCTGAACTGGAGCTGGAGAAGTCCAAGCCCAAGCAGATATCCGACCAGGCCATGCGCAAGAAGATGGCCAGGGCGCACGACGGGATCCTCAAGTACATGCTCAAGCTGATGGAGGTGTGCAATGCCCGTGGGTTTGTCTATGGGATCATCCCTGAGAAAGGGAAGCCTGTGAGTGGTGCGTCGGATAATATTAGAGCTTGGTGGAAGGAGAAGGTTAAGTTTGATAAGAATGGTCCGGCAGCGATTGCAAAGTACGAGGCCGAGCACTTGGTGGATGCTGATGCTCAGAGTAGCGTTGTCAAGAACGAGCACAGCTTGATGGATCTCCAAGACGCCACTCTGGGCTCACTGCTTTCATCATTGATGCAGCACTGTAATCCACCGCAGCGCAAGTACCCCCTGGAGAAGGGCACTCCGCCCCCATGGTGGCCTGCGGGGAATGAAGAGTGGTGGGCTGCCTTGGGCCTTCCAAGGGGACAGATTGCTCCTTACAAAAAACCTCATGACCTTAAGAAGGTTTGGAAGGTCGGGGTGCTTACATGTGTTATTAAGCACATGTCCCCTAACTTTGATAAGATCAGAAACCATGTTCGCAAATCCAAAATCTTGCAGGATAAAATGACTGCAAAGGAGAGCCTGATTTGGTTGGGAGTCCTACAGAGAGAGGAAAGACTTGTGCACGGTATTGACAATGGCGTCTCAGAGATTACTCACCGCAGTGCTCCAGAAGACAGAAATGGAACTAGGAACGCACACAGCAGCAGTAATGAGTATGATGTTGATGGTTTTGAGGAGGCTCCTCTTTCAATATCATCTAAAGATGATGAGCAAGGTCTTTCTCCAGCTGCACAATCCAGCGAGGAGCATGTCTCCAGAAGAGACAGAGAAAGGGCGAACACTAAACATCTTTATCAGGCTGTTGTTCTTAAGGAAGGAACAAAAAAACAACCACCAAAGAGAAAAAGAGCACGTCACAGTTCCGTTGCTGTTGAGCAGGAGGTACAAAGGACTGATGATGCACCAGAAAATCCAGGAAATCTGATTCCCGATATGAACCGACTGGACCAAGTAGAGATTCCAGGCATGGCTAACCACCAGATTACAAGCTTCAATCAGATGGCCGTTACAAGTGAAGCTTTACAACACAGAGGAAATGCTCAAGGGCATGTTTATCTTCCTGGGTCTGGGGTTAATGGCTTTGATAATGCCCAACCTGTGGATGCTACTCCTGTAAGCATATATCAGCCTGTACTTTATGGAAGTAGTGATAATGCCAGGTCAAAGTCTGGAAGCCCCTTTCCACTGCATGCCAATTCTGGTTTTAATAGTTTTCCCAGTAACTATCAGACTTTACCTCCGAAACAATCAGTGCCATTACCTATGATGGATCATCATGTGGTTCCCATGGGTATCAGGGCACCAGCTGACAACAGTCCTTATGGTGATCATGTGATTGGTGGTGGAAGTTCAACTTCTGTCCCTGGAGATATGCAACACCTCGTAGATTTTCCATTCTACGGTGAGCAAGATAAGTTTGTTGGCAGTTCTTTTGAGGGGTTACCTTTGGACTATATCAGTATGAGCAGCCCGATCCCAGATATTGATGATTTGCTGCTGCATGATGATGATTTAATGGAATACCTGGGAACATAA
Protein Sequence:
- >EMT14735|Aegilops_tauschii|EIL|EMT14735
MDQLALLATEFGDSSDFEVDGINENDVSDEEIEPEELARRMWKDRVRLRRIKERQQRLALQQAELELEKSKPKQISDQAMRKKMARAHDGILKYMLKLMEVCNARGFVYGIIPEKGKPVSGASDNIRAWWKEKVKFDKNGPAAIAKYEAEHLVDADAQSSVVKNEHSLMDLQDATLGSLLSSLMQHCNPPQRKYPLEKGTPPPWWPAGNEEWWAALGLPRGQIAPYKKPHDLKKVWKVGVLTCVIKHMSPNFDKIRNHVRKSKILQDKMTAKESLIWLGVLQREERLVHGIDNGVSEITHRSAPEDRNGTRNAHSSSNEYDVDGFEEAPLSISSKDDEQGLSPAAQSSEEHVSRRDRERANTKHLYQAVVLKEGTKKQPPKRKRARHSSVAVEQEVQRTDDAPENPGNLIPDMNRLDQVEIPGMANHQITSFNQMAVTSEALQHRGNAQGHVYLPGSGVNGFDNAQPVDATPVSIYQPVLYGSSDNARSKSGSPFPLHANSGFNSFPSNYQTLPPKQSVPLPMMDHHVVPMGIRAPADNSPYGDHVIGGGSSTSVPGDMQHLVDFPFYGEQDKFVGSSFEGLPLDYISMSSPIPDIDDLLLHDDDLMEYLGT