Gene Details:

Protein Features:

Annotation Proteins:

  • Refseq:  XP_006410269.1  — probable transcription factor PosF21
  • Refseq:  XP_006410270.1  — probable transcription factor PosF21
  • Swissprot:  Q04088  — POF21_ARATH; Probable transcription factor PosF21
  • TrEMBL:  E4MXN6  — E4MXN6_EUTHA; mRNA, clone: RTFL01-24-D07
  • TrEMBL:  V4M9E9  — V4M9E9_EUTSA; Uncharacterized protein
  • STRING:  Bostr.24513s0082.1.p  — (Boechera stricta)
  • STRING:  XP_006410269.1  — (Eutrema salsugineum)

Gene Ontology:

  • GO:0006355  — Biological Process — regulation of transcription, DNA-templated
  • GO:0005634  — Cellular Component — nucleus
  • GO:0005829  — Cellular Component — cytosol
  • GO:0003700  — Molecular Function — transcription factor activity, sequence-specific DNA binding
  • GO:0043565  — Molecular Function — sequence-specific DNA binding

Family Introduction:

  • The bZIP domain consists of two structural features located on a contiguous alpha-helix: first, a basic region of ~ 16 amino acid residues containing a nuclear localization signal followed by an invariant N-x7-R/K motif that contacts the DNA; and, second, a heptad repeat of leucines or other bulky hydrophobic amino acids positioned exactly nine amino acids towards the C-terminus, creating an amphipathic helix. To bind DNA, two subunits adhere via interactions between the hydrophobic sides of their helices, which creates a superimposing coiled-coil structure. The ability to form homo- and heterodimers is influenced by the electrostatic attraction and repulsion of polar residues flanking the hydrophobic interaction surface of the helices.
  • Plant bZIP proteins preferentially bind to DNA sequences with an ACGT core. Binding specificity is regulated by flanking nucleotides. Plant bZIPs preferentially bind to the A-box (TACGTA), C-box (GACGTC) and G-box (CACGTG), but there are also examples of nonpalindromic binding sites.

Literature:

Sequences:

CDS Sequence:
  • >Rsa1.0_00385.1_g00001.1|Raphanus_sativus|bZIP|Rsa1.0_00385.1_g00001.1
    ATGGATAAAGAGAAATCTCCAGCACCTTGTGGAGGTCGTCTCCCTCCTCCATCTCCTTCAGGTCGCTGCTCACCCTTCTCAGATATGAGCCGTATGCTCGAACACCCACCTAAGAAGATCGGACACCGTCGAGCTCACTCAGAGATCCTCACTCTCCCTGATGACTTGAGCTTTGATTTTGATCTTGGTTTGGTTGGTAATGGTAGTGGTACTAATGCTGATGTGCCTTCTTTCTCTGATGACACTGAAGGAGGTGGGGACTTGCTGTCTATGTATCTCGATATGGATAAGTTTAATTCTTCTACTGCTACTTCTTCTTCGGCGGCTCAAGTGGGTGAGCCGTCAGGAACCGCTTGGAAAAACGAGTTGGTGGTGACGCATACAGGCTCAACTTCTTCTAATCATCAGAACAGTAGTTTTGGGGAAAGGCCAAGAGTTAGGCATCAGCACAGTCACTCTATGGATGGATCATTGAGCATCAGTGAAATGCTTATGTCTGGAAATGAAGATGATGCTAAGAAGTCCATCTCTGCTGCTAAACTTGCTGAGCTTGCTCTCATCGATCCAAAACGTGCTAAGAGGATATGGGCAAACAGGCAGTCAGCGGCAAGGTCAAAGGAAAGGAAGACGAGATACATATTTGAGCTTGAGAGAAAAGTACAGACTTTGCAAACTGAAGCTACAACTCTCTCTGCCCAGCTTACTCTCTTGCAGAGAGACGCAAATGGTTTGAGTGTTGAAAACAATGAGCTGAAACTGAGGTTGCAAACAATGGAGCAGAAAGTTCACTTACAGGATGAACTAAACGAAGCTCTTAAGGAGGAAATCCAGCATCTGAAAGTGTTGACTGGCCAAGTTGCTGCCCCAAACGGTGCATCATCAGCAATGAACTATGGGTCGTTTGGATCAAACCAGCAATTATATTCCAACAATCATCAGTCAATGCAGACGATGTTAGCCGCACAACAGCTCCAGCAGCTGCAGATTCATTCACAGAAACAACAACAGCAGGAGTTTCAGTTTCAACAGCAGCAGCAGTTTCTGCATCATCGCCTTCGACAGCAAGAACAACAAAATGGAATTGCGGAGATGGAGAAAACAGAATGA
Protein Sequence:
  • >Rsa1.0_00385.1_g00001.1|Raphanus_sativus|bZIP|Rsa1.0_00385.1_g00001.1
    MDKEKSPAPCGGRLPPPSPSGRCSPFSDMSRMLEHPPKKIGHRRAHSEILTLPDDLSFDFDLGLVGNGSGTNADVPSFSDDTEGGGDLLSMYLDMDKFNSSTATSSSAAQVGEPSGTAWKNELVVTHTGSTSSNHQNSSFGERPRVRHQHSHSMDGSLSISEMLMSGNEDDAKKSISAAKLAELALIDPKRAKRIWANRQSAARSKERKTRYIFELERKVQTLQTEATTLSAQLTLLQRDANGLSVENNELKLRLQTMEQKVHLQDELNEALKEEIQHLKVLTGQVAAPNGASSAMNYGSFGSNQQLYSNNHQSMQTMLAAQQLQQLQIHSQKQQQQEFQFQQQQQFLHHRLRQQEQQNGIAEMEKTE