Gene Details:

Protein Features:

Annotation Proteins:

  • Refseq:  XP_018450922.1  — PREDICTED: ABSCISIC ACID-INSENSITIVE 5-like protein 6
  • Swissprot:  Q9M7Q3  — AI5L6_ARATH; ABSCISIC ACID-INSENSITIVE 5-like protein 6
  • TrEMBL:  E4MY11  — E4MY11_EUTHA; mRNA, clone: RTFL01-30-J01
  • TrEMBL:  V4MBG2  — V4MBG2_EUTSA; Uncharacterized protein
  • STRING:  XP_006412286.1  — (Eutrema salsugineum)

Gene Ontology:

  • GO:0006355  — Biological Process — regulation of transcription, DNA-templated
  • GO:0003700  — Molecular Function — transcription factor activity, sequence-specific DNA binding
  • GO:0043565  — Molecular Function — sequence-specific DNA binding

Family Introduction:

  • The bZIP domain consists of two structural features located on a contiguous alpha-helix: first, a basic region of ~ 16 amino acid residues containing a nuclear localization signal followed by an invariant N-x7-R/K motif that contacts the DNA; and, second, a heptad repeat of leucines or other bulky hydrophobic amino acids positioned exactly nine amino acids towards the C-terminus, creating an amphipathic helix. To bind DNA, two subunits adhere via interactions between the hydrophobic sides of their helices, which creates a superimposing coiled-coil structure. The ability to form homo- and heterodimers is influenced by the electrostatic attraction and repulsion of polar residues flanking the hydrophobic interaction surface of the helices.
  • Plant bZIP proteins preferentially bind to DNA sequences with an ACGT core. Binding specificity is regulated by flanking nucleotides. Plant bZIPs preferentially bind to the A-box (TACGTA), C-box (GACGTC) and G-box (CACGTG), but there are also examples of nonpalindromic binding sites.

Literature:

Sequences:

CDS Sequence:
  • >Rsa1.0_00344.1_g00006.1|Raphanus_sativus|bZIP|Rsa1.0_00344.1_g00006.1
    ATGGGTTCTCGATTTAACTTCGTTGATGAGGCAAAGCATCCAGCTTTGGGGGCTGGTGTTCCATTGATGAGACAGAACTCTGTTTTCTCGTTAACCTTTGATGAGTTTCAGAGCTCATGGGGTGGTGGGATTGGGAAGGACTTTGGGTCCATGAACATGGACGAGCTCTTGAAGAACATTTGGACAGCTGAGGAAAGCCACTCTATGATGGCCAACAACAATAACACCAGTTTCAACAACACTGTTGTTATGAACAACAATGGAGGTTTCTCTGTTGGTGTGGGAGGAGAACTTGCCGGTTGTGGTGGTGGTTTGCAGAGACAAGGGTCACTTACGTTGCCTCGTACCATTAGTCAGAAAAGAGTTGATGATGTCTGGAAGGAGCTGATGAAGGAGGATGACAGTGGAGATGGTGTAGGTAACGTTGGAGGAGGAGCAAGTGGAGTTCCGCAGAGGCAACAGACGTTGGGAGAGATGACTCTGGAGGAGTTTTTGGTCAGAGCCGGTGTGGTTAGGGAAGAGTCTCAACCGGTGGAGAGGGTGGATAACTTCAATGGTGGGTTCTTTGGATTTGGAAGTGATGCAGGTTTGGTTTCAGCTCGTAATGGGTTTGGTGGTCCCAACCAGCCTCATGGAGGTGTGGTGAGACCGGATCTGCTGACAACTCAAACTCAGCCACTGCAGATGCAGCAGCAGCAGCCACAGAAAGTGCATCAGCCGCGAGAACTGATACCGAAGCAGGATGTAGCTTTCCCCAAACAGACTACTATAGCGTTTTCCAACACTGTTGATCTGGTCAACCGTTCTCAACCTCTAACACAGGAGGTGAAGCCTACAATTCTTGGAGTTAGGGACAGGCCTATGAACAACAACCTTCTTCAAGCTGTTGATTTCAAAACAGGTGTTACGGTTGCAGCAGTATCTCCCGGAAGCCAGATGTCACCTGATCTGACTCCAAAGAGCGCCATGGATGCATCTTTGTCACCAGTTCCTTACATGTTTGGTCGTGTGAGAAAAACAGGCGCAGTCCTGGAGAAAGTGATTGAGAGGAGGCAGAAAAGGATGATTAAGAATAGGGAATCAGCTGCAAGATCTCGCGCTCGCAAGCAAGCTTATACGTTGGAGTTGGAGGCAGAAGTTGCACAACTGAAAGAGCAGAATGAAGAGTTGCAGAGGAAACAAGTTGAAATCATGGAAAAGCAGAAAAAACAGCTTCTGGAGCCAATGCGTCAGGCATGGGGATGCAAAAGGCAATGCTTGCGAAGGACATTGACGGGTCCTTGGTAG
Protein Sequence:
  • >Rsa1.0_00344.1_g00006.1|Raphanus_sativus|bZIP|Rsa1.0_00344.1_g00006.1
    MGSRFNFVDEAKHPALGAGVPLMRQNSVFSLTFDEFQSSWGGGIGKDFGSMNMDELLKNIWTAEESHSMMANNNNTSFNNTVVMNNNGGFSVGVGGELAGCGGGLQRQGSLTLPRTISQKRVDDVWKELMKEDDSGDGVGNVGGGASGVPQRQQTLGEMTLEEFLVRAGVVREESQPVERVDNFNGGFFGFGSDAGLVSARNGFGGPNQPHGGVVRPDLLTTQTQPLQMQQQQPQKVHQPRELIPKQDVAFPKQTTIAFSNTVDLVNRSQPLTQEVKPTILGVRDRPMNNNLLQAVDFKTGVTVAAVSPGSQMSPDLTPKSAMDASLSPVPYMFGRVRKTGAVLEKVIERRQKRMIKNRESAARSRARKQAYTLELEAEVAQLKEQNEELQRKQVEIMEKQKKQLLEPMRQAWGCKRQCLRRTLTGPW