Gene Details:

Protein Features:

Annotation Proteins:

  • Refseq:  XP_018442824.1  — PREDICTED: basic leucine zipper 6
  • Refseq:  XP_018442825.1  — PREDICTED: basic leucine zipper 6
  • TrEMBL:  A0A3P6EAD3  — A0A3P6EAD3_BRAOL; Uncharacterized protein
  • STRING:  Bo9g179040.1  — (Brassica oleracea)

Family Introduction:

  • The bZIP domain consists of two structural features located on a contiguous alpha-helix: first, a basic region of ~ 16 amino acid residues containing a nuclear localization signal followed by an invariant N-x7-R/K motif that contacts the DNA; and, second, a heptad repeat of leucines or other bulky hydrophobic amino acids positioned exactly nine amino acids towards the C-terminus, creating an amphipathic helix. To bind DNA, two subunits adhere via interactions between the hydrophobic sides of their helices, which creates a superimposing coiled-coil structure. The ability to form homo- and heterodimers is influenced by the electrostatic attraction and repulsion of polar residues flanking the hydrophobic interaction surface of the helices.
  • Plant bZIP proteins preferentially bind to DNA sequences with an ACGT core. Binding specificity is regulated by flanking nucleotides. Plant bZIPs preferentially bind to the A-box (TACGTA), C-box (GACGTC) and G-box (CACGTG), but there are also examples of nonpalindromic binding sites.

Literature:

Sequences:

CDS Sequence:
  • >Rsa1.0_00123.1_g00037.1|Raphanus_sativus|bZIP|Rsa1.0_00123.1_g00037.1
    ATGTCAAGGCCTGCTCAGCTTCCTCCTCGTTGCCCCATTCCAAAGAAGCTCTCTTTGAATCCGTTTGCAGACACCTACTACTCTTCTTCTTCCCCCGTTGAGTCATACATTGCCCATCACAAGCCTCCCACTCAAGACTCCACGCTCGAGGAACAACCAGCTTGGCTCGATGAGCTGCTGCGTGACAAAACTGATGGGATGATGCTGCTCACTGGTGGTGGAGGAGGAGGAGGTCCTCCTCCTCCTCTGCCTCTGCGCCGCTCAGCTAGCGACTCGGTTGTGCTGCTGGGAGACATCTCGGCGGATTTTGCTGCTGGTTTTAATCAAAGCGAGGATGAAGAGAGTTTGACTTCTGAAGCTTGTGGGGAGTTGGAGTCAGCTTGTGTGTATGGTCCCAACTCGCCTAGGGCAAAGAACAGCTCAAACTTCTCTGACAACCCTATTGCTTCTGCTTTCTCGGATTATGGTTCTCAGAAAGCTCGTCAGAACTCGGATGATGCTTTGAAAGGAAGCAATGTTCCACCTGTGGAAGAAAATGCTTGCGGTTCAATGGGCGTACCGAATGCCAAAAGATCAAGACTGATATATAACGGAAGGGGTACACTTTGGTTTATCCTTGGATTTGTAGTTAAACATAGGCTTGTTCTGGGCTACATTGGCAAATTTGTAGATGACCTTTGGAACCCAGGACAGCGTTCGAGAGTTCGTAAGCTCCAGTACATTGCTGAACTTGAGAGGAGGGTATCCATGCTGCAGGCAGTGGAAGCGGATTTATCAGTGAGAGTGGCTTCACTTGTTCAGACACGTGCAACACTGTCCTTGGAGAATAGCCAGATGAAGCAGCAGATGGCGATACTAAAGCAGGACAAGCTCATAAGAGAAGGTGAATACCAGTTACTAAAGAAGGAAGCTCAGAGATTGAACTCCAGATTGAGAAACATTGGGAGCAACAACAACAACAGGCTGGCCAGGTCATATTCTGCCGGTTCAAGTATAGGTCCAAGGACTGCTTCGTCACATTTGGACTGGAACTTGCTGGACCTGACCAAACTCAATCTCAACTAA
Protein Sequence:
  • >Rsa1.0_00123.1_g00037.1|Raphanus_sativus|bZIP|Rsa1.0_00123.1_g00037.1
    MSRPAQLPPRCPIPKKLSLNPFADTYYSSSSPVESYIAHHKPPTQDSTLEEQPAWLDELLRDKTDGMMLLTGGGGGGGPPPPLPLRRSASDSVVLLGDISADFAAGFNQSEDEESLTSEACGELESACVYGPNSPRAKNSSNFSDNPIASAFSDYGSQKARQNSDDALKGSNVPPVEENACGSMGVPNAKRSRLIYNGRGTLWFILGFVVKHRLVLGYIGKFVDDLWNPGQRSRVRKLQYIAELERRVSMLQAVEADLSVRVASLVQTRATLSLENSQMKQQMAILKQDKLIREGEYQLLKKEAQRLNSRLRNIGSNNNNRLARSYSAGSSIGPRTASSHLDWNLLDLTKLNLN