Gene Details:

  • Gene ID: Pgl016280
  • Gene Family: bZIP Family
  • Description: bZIP Family protein
  • Species: Picea glauca
  • Source: bZIP family gene from PlantTFDB

Protein Features:

Annotation Proteins:

  • Refseq:  XP_020526256.1  — bZIP transcription factor TGA10 isoform X1
  • Refseq:  XP_020526257.1  — bZIP transcription factor TGA10 isoform X2
  • Refseq:  XP_020526258.1  — bZIP transcription factor TGA10 isoform X3
  • Swissprot:  Q52MZ2  — TGA10_TOBAC; bZIP transcription factor TGA10
  • TrEMBL:  A0A0K0M741  — A0A0K0M741_PINTB; ZIP2 (Fragment)
  • STRING:  ERN11662  — (Amborella trichopoda)
  • STRING:  EFJ22847  — (Selaginella moellendorffii)

Gene Ontology:

  • GO:0006351  — Biological Process — transcription, DNA-templated
  • GO:0043565  — Molecular Function — sequence-specific DNA binding

Family Introduction:

  • The bZIP domain consists of two structural features located on a contiguous alpha-helix: first, a basic region of ~ 16 amino acid residues containing a nuclear localization signal followed by an invariant N-x7-R/K motif that contacts the DNA; and, second, a heptad repeat of leucines or other bulky hydrophobic amino acids positioned exactly nine amino acids towards the C-terminus, creating an amphipathic helix. To bind DNA, two subunits adhere via interactions between the hydrophobic sides of their helices, which creates a superimposing coiled-coil structure. The ability to form homo- and heterodimers is influenced by the electrostatic attraction and repulsion of polar residues flanking the hydrophobic interaction surface of the helices.
  • Plant bZIP proteins preferentially bind to DNA sequences with an ACGT core. Binding specificity is regulated by flanking nucleotides. Plant bZIPs preferentially bind to the A-box (TACGTA), C-box (GACGTC) and G-box (CACGTG), but there are also examples of nonpalindromic binding sites.

Literature:

Sequences:

CDS Sequence:
  • >Pgl016280|Picea_glauca|bZIP|gnl|UG|Pgl#S55349454
    GCTTTAAATCGAGAGGCTGCTCGGAAAAGCAGATTGAGGAAGAAGGCATACGTTCAGCAATTAGAAAGCAGTCGAATAAAACTGGCCCAGCTTGAGCAAGAGCTTCAGCGGGCTCGAACACAGGGAGTATTTCTAGGATCGAACGGGTACCTGAATGATCAGGGTCATCTCACTGGGAATGCTGTCTCCAATGGAAGCCTAACCTCTGCTGCTGCCTTCGATATGGAATACCAACGATGGCTAGAAGAGCACCACCGTCACATGTGTGATCTTCGAGCAGCATTGCATGAACATCTGCCAGACAATGATCTCCGCGTGTTTGTAGAGAACTGCCTAGCTCATTACGATGATCTCTTACACATCAAGATTGTCGGTGCCAAGGCAGACGTTTTCTATCTGGTTTCAGGGATGTGGAAGACCCCCGCAGAACGCTTTTTCATGTGGATGGGAGGCTTCCGGCCGTCAGAAATTCTCAAGATCCTGATTCCACAGATAGAACCGTTGACAGAGCAACAGTATCAATGCATTTGTAGTCTGCAGCAGTCTTCACTCCAAGCCGAAGAGGCCTTTTCTCAGGGCATGGAAGAGCTTCAACACTCTATTTCCGAGAACATAGCTGCAGGATCCCTCAGCTCCCCTTCAAACATGGCCAATTATATGGGACAAATGGCCATCGTCATGGGAAAACTTGCCAATCTAGAAATCTTGGCGGATAGTCTGAGACATCAGACATTACAACAGCTGCACAGAATCCTCACCACGCGCCAGTCAGCCAAAAGCTTACTTTCAATAGGAGAATACTGCTCTCGTCTTCGTGCACTGAGCTCTCTTTGGTCAGCTAGACCCAGAGAATGA
Protein Sequence:
  • >Pgl016280|Picea_glauca|bZIP|gnl|UG|Pgl#S55349454
    ALNREAARKSRLRKKAYVQQLESSRIKLAQLEQELQRARTQGVFLGSNGYLNDQGHLTGNAVSNGSLTSAAAFDMEYQRWLEEHHRHMCDLRAALHEHLPDNDLRVFVENCLAHYDDLLHIKIVGAKADVFYLVSGMWKTPAERFFMWMGGFRPSEILKILIPQIEPLTEQQYQCICSLQQSSLQAEEAFSQGMEELQHSISENIAAGSLSSPSNMANYMGQMAIVMGKLANLEILADSLRHQTLQQLHRILTTRQSAKSLLSIGEYCSRLRALSSLWSARPRE