Gene Details:
- Gene ID: GSBRNA2T00143541001
- Gene Name: GSBRNA2T00143541001, LOC106383049
- Gene Family: bZIP Family
- Description: bZIP Family protein
- Species: Brassica napus
- Source: bZIP family gene from PlantTFDB
Protein Features:
- SMART: SM00338
- Gene3D: G3DSA:1.20.5.170
- PROSITE profile: PS50217
- Pfam: PF00170
- SuperFamily: SSF57959
- PROSITE pattern: PS00036
- InterPro: IPR004827
Annotation Proteins:
- Refseq: XP_009125331.1 — PREDICTED: transcription factor TGA4 isoform X1
- Refseq: XP_009125332.1 — PREDICTED: transcription factor TGA4 isoform X1
- Refseq: XP_013678618.1 — transcription factor TGA4
- Refseq: XP_013678625.1 — transcription factor TGA4
- Refseq: XP_013678633.1 — transcription factor TGA4
- Refseq: XP_018512473.1 — PREDICTED: transcription factor TGA4 isoform X1
- Refseq: XP_018512474.1 — PREDICTED: transcription factor TGA4 isoform X1
- Swissprot: Q39162 — TGA4_ARATH; Transcription factor TGA4
- TrEMBL: A0A3P6A6G7 — A0A3P6A6G7_BRACM; Uncharacterized protein
- TrEMBL: M4EII9 — M4EII9_BRARP; Uncharacterized protein
- STRING: Bra028604.1-P — (Brassica rapa)
Gene Ontology:
- GO:0006355 — Biological Process — regulation of transcription, DNA-templated
- GO:0009409 — Biological Process — response to cold
- GO:0042742 — Biological Process — defense response to bacterium
- GO:0005634 — Cellular Component — nucleus
- GO:0003700 — Molecular Function — transcription factor activity, sequence-specific DNA binding
- GO:0043565 — Molecular Function — sequence-specific DNA binding
Family Introduction:
- The bZIP domain consists of two structural features located on a contiguous alpha-helix: first, a basic region of ~ 16 amino acid residues containing a nuclear localization signal followed by an invariant N-x7-R/K motif that contacts the DNA; and, second, a heptad repeat of leucines or other bulky hydrophobic amino acids positioned exactly nine amino acids towards the C-terminus, creating an amphipathic helix. To bind DNA, two subunits adhere via interactions between the hydrophobic sides of their helices, which creates a superimposing coiled-coil structure. The ability to form homo- and heterodimers is influenced by the electrostatic attraction and repulsion of polar residues flanking the hydrophobic interaction surface of the helices.
- Plant bZIP proteins preferentially bind to DNA sequences with an ACGT core. Binding specificity is regulated by flanking nucleotides. Plant bZIPs preferentially bind to the A-box (TACGTA), C-box (GACGTC) and G-box (CACGTG), but there are also examples of nonpalindromic binding sites.
Literature:
Sequences:
CDS Sequence:
- >GSBRNA2T00143541001|Brassica_napus|bZIP|GSBRNA2T00143541001
ATGAATACAACTTCAACACATTTTGTTCCTCCGAGGAGATTTGAAATCTACGAGACTACTCTCAACCAAGTCAGTATGTGGGAAGAGAGTTTCAAGAACAATGGAGGCATATTTACACCTAACTCTATCATCATCCCCACAGATGCAAAACTAGACAGCTTGTCTGAGGATACTTCTCATGGAACTCCTCACAAGTTTGACCAAGAAGCTTCCACATCTAGACATCCTGACAAAACACAGAGACGGCTAGCACAGAACCGGGAGGCAGCTAGGAAAAGTCGTTTGCGCAAAAAAGCTTATGTTCAGCAGCTAGAGACAAGCAGGTTGAAGCTAATTCATTTAGAGCAAGAACTTGATCGTGCTAGACAACAAGGTTTCTATGAAGGGAACCGAGTAGATACTAATGCTCTTGGTTTCTCAGACAAGATGAGCTCAGGGATTGTAGCATTTGAGATGGAATATGGACACTGGGTGGAAGAACAGAACAGGCAAATAAGCGAGCTGAGAACCGTTTTACAAGGACAAGTTAGTGATGTAGAGCTTCGTTTGCTAGTTGACAATGCCATGAAACATTACTTTCAACTCTTCCGAATGAAGTCAGCAGCTGCAAAGATCGATGTCTTCTACATCATGTCCGGAATGTGGAAAACTTCAGCAGAGAGGTTTTTCATGTGGATAGGTGGGTTCAGACCCTCGGAGCTCCTCAAGGTTCTGTTACCACATTTTGATCCTTTGATGGATCAACAAGTATTGGATGTGTGCAATCTGAGGCAATCATGTCAACAAGCTGAAGATGCTGTATCTCAAGGCATGGAGAAGCTGCAGCATACATTAGCAGATAGTGTAGCAGCGGGGAATCTCGGTGAAGGAAGTTACATTCCTCAAATAACTTGTGCTATGGAGAGATTGGAAGCTTTGGTCAGTTTTGTGAATCAGGCTGATCATCTTAGACATGAGACATTGCAACAGATGCATCGGATCTTAACCACTAGACAAGCGGCTAGGGGTTTGTTAGCATTAGGTGAGTATTTCCAACGGTTAAGAGCTTTAAGCTCGAGTTGGGCGACTCGGCAACGTGAACCAACGTAA
Protein Sequence:
- >GSBRNA2T00143541001|Brassica_napus|bZIP|GSBRNA2T00143541001
MNTTSTHFVPPRRFEIYETTLNQVSMWEESFKNNGGIFTPNSIIIPTDAKLDSLSEDTSHGTPHKFDQEASTSRHPDKTQRRLAQNREAARKSRLRKKAYVQQLETSRLKLIHLEQELDRARQQGFYEGNRVDTNALGFSDKMSSGIVAFEMEYGHWVEEQNRQISELRTVLQGQVSDVELRLLVDNAMKHYFQLFRMKSAAAKIDVFYIMSGMWKTSAERFFMWIGGFRPSELLKVLLPHFDPLMDQQVLDVCNLRQSCQQAEDAVSQGMEKLQHTLADSVAAGNLGEGSYIPQITCAMERLEALVSFVNQADHLRHETLQQMHRILTTRQAARGLLALGEYFQRLRALSSSWATRQREPT*