Gene Details:

  • Gene ID: GSBRNA2T00078593001
  • Gene Name: GSBRNA2T00078593001, LOC106397420
  • Gene Family: bZIP Family
  • Description: bZIP Family protein
  • Species: Brassica napus
  • Source: bZIP family gene from PlantTFDB

Protein Features:

Annotation Proteins:

  • Refseq:  XP_013616314.1  — PREDICTED: transcription factor TGA5
  • Refseq:  XP_013693455.1  — transcription factor TGA5
  • Swissprot:  Q39163  — TGA5_ARATH; Transcription factor TGA5
  • TrEMBL:  A0A078FLY2  — A0A078FLY2_BRANA; BnaC02g01780D protein
  • TrEMBL:  A0A0D3AI12  — A0A0D3AI12_BRAOL; Uncharacterized protein
  • STRING:  Bo2g007460.1  — (Brassica oleracea)

Gene Ontology:

  • GO:0006355  — Biological Process — regulation of transcription, DNA-templated
  • GO:0009410  — Biological Process — response to xenobiotic stimulus
  • GO:0009863  — Biological Process — salicylic acid mediated signaling pathway
  • GO:0003700  — Molecular Function — transcription factor activity, sequence-specific DNA binding
  • GO:0042802  — Molecular Function — identical protein binding
  • GO:0043565  — Molecular Function — sequence-specific DNA binding

Family Introduction:

  • The bZIP domain consists of two structural features located on a contiguous alpha-helix: first, a basic region of ~ 16 amino acid residues containing a nuclear localization signal followed by an invariant N-x7-R/K motif that contacts the DNA; and, second, a heptad repeat of leucines or other bulky hydrophobic amino acids positioned exactly nine amino acids towards the C-terminus, creating an amphipathic helix. To bind DNA, two subunits adhere via interactions between the hydrophobic sides of their helices, which creates a superimposing coiled-coil structure. The ability to form homo- and heterodimers is influenced by the electrostatic attraction and repulsion of polar residues flanking the hydrophobic interaction surface of the helices.
  • Plant bZIP proteins preferentially bind to DNA sequences with an ACGT core. Binding specificity is regulated by flanking nucleotides. Plant bZIPs preferentially bind to the A-box (TACGTA), C-box (GACGTC) and G-box (CACGTG), but there are also examples of nonpalindromic binding sites.

Literature:

Sequences:

CDS Sequence:
  • >GSBRNA2T00078593001|Brassica_napus|bZIP|GSBRNA2T00078593001
    ATGGGAGATACTAGTCCAAGAACATCAGGCTCAACAGATGGCGACATGGATCAAAACAACCTAATGTACGATGGAGGGCATGTGGGTGAATCTAGCGACCGTTCAAAGGAAAAAATGGATCAAAAGACGGTTCGTAGGCTCGCTCAAAACCGTGAGGCTGCAAGGAAAAGCAGATTGAGGAAGAAAGCATATGTTCAGCAGCTAGAGAACAGCCGTTTGAAGCTAACACAGCTTGAACAGGAGCTGCAAAGAGCAAGGCAACAAGGTGTCTTTATCTCAAGCTCTGGAGACCAAGCCCATTCAACTACTGGAAATGGGGCAATGGCGTTTGATGCAGAGTACAGACGCTGGCAGGAAGATAAGAACAGAAAGATGAAGGAGCTTAGTTCTGCTTTGGATTCTCACGCGAGTGAACCTGAGCTTAGGACAATCGTAGAAGCAGTGTTGGCTCACTACGAGGAGCTTTTCAGGATAAAAAGCAACGCAGCTAAGAACGATGTCTTCCATTTACTATCAGGGATGTGGAAAACACCAGCTGAGAGATGTTTCCTGTGGCTTGGCGGTTTCCGTTCATCAGACCTTCTCAAGCTTATAGCGAGTCAGGTGGAGCCATTGACGGAACAACAATCACAAGACCTAAACAACTTGCAAGAATCTTCTCAACAAGCGGAAGATGCTTTGTCTCAAGGGATGGACAGTTTACAGCAATCACTCTCCGAGACTCTATCTAGCGGAACTCTTGGTTCAAGCTCGTCAGGGAACGTGGCTAACTACATGGGTCAGATGGCAATGGCCATGGGGAAGTTAGGTACACTTGAAGGATTCATCCGCCAGGCTGATAACTTGAGGCTACAAACGTATCAACAAATGCTGAGAAAGTTGACAACCAGACAATCAGCTAGGGCTCTCCTTGCGATACATGAGTATTCGTTGCGGTTACGTGCTCTTAGCTCTCTATGGCTTGCTAGACCAAGAGAGTGA
Protein Sequence:
  • >GSBRNA2T00078593001|Brassica_napus|bZIP|GSBRNA2T00078593001
    MGDTSPRTSGSTDGDMDQNNLMYDGGHVGESSDRSKEKMDQKTVRRLAQNREAARKSRLRKKAYVQQLENSRLKLTQLEQELQRARQQGVFISSSGDQAHSTTGNGAMAFDAEYRRWQEDKNRKMKELSSALDSHASEPELRTIVEAVLAHYEELFRIKSNAAKNDVFHLLSGMWKTPAERCFLWLGGFRSSDLLKLIASQVEPLTEQQSQDLNNLQESSQQAEDALSQGMDSLQQSLSETLSSGTLGSSSSGNVANYMGQMAMAMGKLGTLEGFIRQADNLRLQTYQQMLRKLTTRQSARALLAIHEYSLRLRALSSLWLARPRE*