Gene Details:

Functional Descriptions:

  • Natural variations in the Sl-AKR9 aldo/keto reductase gene impact fruit flavor volatile and sugar contents.
  • Identified a gene encoding a tomato aldo/keto reductase, Sl-AKR9, that is associated with phenylacetaldehyde and 2-phenylethanol contents in fruits.
  • Sl-AKR9 effectively catalyzes reduction of phenylacetaldehyde to 2-phenylethanol. The enzyme can also metabolize sugar-derived reactive carbonyls, including glyceraldehyde and methylglyoxal.
  • CRISPR-Cas9-induced loss-of-function mutations in Sl-AKR9 significantly increased phenylacetaldehyde and lowered 2-phenylethanol content in ripe fruit.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Solyc09g011240.3.1
    CGACCACGTGTCAGCTTCAGAAGGTAACAGTTGAAACTTGCACACGATCTTCATTTCATTCACATATATTAAGTGAGTGGAAGTGAAAAGTTCAATTCGCATTGCTACTCTCTACTCATCAATCACTCTTTCTCTCTTTGGCTGTATACGATTCACTCTCGTTTTTTTCAATTTCCCTCCTCTTTTTTTGTATCAATGGCGGAGGCGACGGAGATGCCGTACATTGAGTTGAATACCGGTTTCAGCATTCCGGCGGTCGGTTTGGGAACGTGGCAGTCCGATCCCGGTGTCGTCGGCAAAGCCGTGGAGACTGCTATCAAGATGGGCTACCGGCATATTGATTGTGCTCAAATTTATAAAAATGAGAAGGAGATTGGTGAAGTATTGAGCAGGCTGTTTAAAGATGGTGTTGTCAAGAGGCGAGAGCTATTCATTACATCCAAACTCTGGAACACTAATCACGCACCTGAAGATGTACCAGTCGCACTAGACAAAACTCTTCAAGACTTGCAGCTGGAATATGTTGACCTGTACCTAATCCATTGGCCTGTCAGTATGAAACCTGGCTCGGTTGACTTTAAACCTGAAAATCTTATGCCAACCAACATACCCAGGATATGGGAAGCAATGGAAAAAGTTTATGATTCCGGGAAGGCTAGAGTTATTGGTGTTAGCAATTTCTCTACTAAGAAACTGGAAGATCTTTTGCAAGTAGCACGCACTCCTCCTGCTGTCAACCAGGTGGAGTGCCATCCTTCGTGGCAACAAGCCAAATTGCGGGAATTATGTAAATCCAATAACGTGCACCTCTCTGCATATTCGCCGCTTGGTTCTCCAGGTACAACATGGCTTAAAAGTGATGTGCTAAAGCAGCCAGCTGTAATCTCAGTTGCTGAGAAATTGGGCAAGACTCCGGCACAGGTTTGTCTTCGTTGGGGCATTCAGATGGGTCAGAGTGTACTTCCTAAGAGCACACATGAAGCACGGATTAAAGAGAACCTTGATGTACTGAACTGGTCTATACCTGATGACTTGCTTGCCAAGTTCTCTGAAATTCCACAGGCAAGGTTGCTGAAAGGTGCTTCATTTGCTCACGAGACGCATGGCCAGTACAGAACACTTGAAGAGCTGTGGGATGGTGAGATTTAGGGTCACAATATGAATTAACTGTTGAATCCATTTGTCTCAGAGATTTAAGCTTCTTGTGCACTTCCTTTTCCTTTAAACTTTTGGAGATAGGTGAATTGTCAAACCATTAGATGTACGTTTCCTTGCACCCCTGTCCCTCTATTGACTCTATGCTGGAAAACCATTCAAGAAACCTCGCAGATTCTACGAGACAGTATTCAAACAATTTGATTTGCTGGGATAATACTACCAAACCATCCACTTTTGCAGTC
CDS Sequence
  • >Solyc09g011240.3.1
    ATGGCGGAGGCGACGGAGATGCCGTACATTGAGTTGAATACCGGTTTCAGCATTCCGGCGGTCGGTTTGGGAACGTGGCAGTCCGATCCCGGTGTCGTCGGCAAAGCCGTGGAGACTGCTATCAAGATGGGCTACCGGCATATTGATTGTGCTCAAATTTATAAAAATGAGAAGGAGATTGGTGAAGTATTGAGCAGGCTGTTTAAAGATGGTGTTGTCAAGAGGCGAGAGCTATTCATTACATCCAAACTCTGGAACACTAATCACGCACCTGAAGATGTACCAGTCGCACTAGACAAAACTCTTCAAGACTTGCAGCTGGAATATGTTGACCTGTACCTAATCCATTGGCCTGTCAGTATGAAACCTGGCTCGGTTGACTTTAAACCTGAAAATCTTATGCCAACCAACATACCCAGGATATGGGAAGCAATGGAAAAAGTTTATGATTCCGGGAAGGCTAGAGTTATTGGTGTTAGCAATTTCTCTACTAAGAAACTGGAAGATCTTTTGCAAGTAGCACGCACTCCTCCTGCTGTCAACCAGGTGGAGTGCCATCCTTCGTGGCAACAAGCCAAATTGCGGGAATTATGTAAATCCAATAACGTGCACCTCTCTGCATATTCGCCGCTTGGTTCTCCAGGTACAACATGGCTTAAAAGTGATGTGCTAAAGCAGCCAGCTGTAATCTCAGTTGCTGAGAAATTGGGCAAGACTCCGGCACAGGTTTGTCTTCGTTGGGGCATTCAGATGGGTCAGAGTGTACTTCCTAAGAGCACACATGAAGCACGGATTAAAGAGAACCTTGATGTACTGAACTGGTCTATACCTGATGACTTGCTTGCCAAGTTCTCTGAAATTCCACAGGCAAGGTTGCTGAAAGGTGCTTCATTTGCTCACGAGACGCATGGCCAGTACAGAACACTTGAAGAGCTGTGGGATGGTGAGATTTAG
Protein Sequence
  • >Solyc09g011240.3.1
    MAEATEMPYIELNTGFSIPAVGLGTWQSDPGVVGKAVETAIKMGYRHIDCAQIYKNEKEIGEVLSRLFKDGVVKRRELFITSKLWNTNHAPEDVPVALDKTLQDLQLEYVDLYLIHWPVSMKPGSVDFKPENLMPTNIPRIWEAMEKVYDSGKARVIGVSNFSTKKLEDLLQVARTPPAVNQVECHPSWQQAKLRELCKSNNVHLSAYSPLGSPGTTWLKSDVLKQPAVISVAEKLGKTPAQVCLRWGIQMGQSVLPKSTHEARIKENLDVLNWSIPDDLLAKFSEIPQARLLKGASFAHETHGQYRTLEELWDGEI*