Gene Details:

Functional Descriptions:

  • The SlHB8 acts as a negative regulator in tapetum development and pollen wall formation in Tomato.
  • Report the isolation of one HD-Zip III family transcription factor, SlHB8, whose expression levels decreased as pollen development progressed.
  • SlHB8 knockout using CRISPR/Cas9 increased pollen activity, subsequently inducing fruit setting, whereas overexpression displayed opposite phenotypes.
  • Overexpression lines under control of the 35 s and p2A11 promoters revealed that SlHB8 reduced pollen activity by affecting early pollen development.
  • The study findings demonstrate that SlHB8 is involved in tapetum development and degradation and plays an important role in anther development.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Solyc08g066500.4.1
    CTTGAAGTGATGATGGCTGTGACATCAAGCTGTAAAGACAAATTTGGGATGGATAGTGGTAAATATGTGAGGTACACTCCTGAGCAGGTAGAGGCTTTGGAGAGGTTGTATCATGAATGTCCAAAGCCTAGTTCACTCCGTCGCCAGCAGTTGATTCGGGAGTGTCCAATTCTGTCGAATATCGAGCCAAAACAGATCAAAGTTTGGTTCCAGAACAGAAGATGTAGAGAGAAGCAACGTAAAGAATCATCGCGCCTTCAAGGCGTGAACAGAAAATTGACTGCAATGAATAAGCTGTTGATGGAAGAGAACGATAGGTTGCAGAAGCAAGTTTCACAGTTGGTGTATGAGAATAGCTTTTTCCGCCAACAGACTCAAACTGCAGCCTTAGCCACCACAGACAACAATAGTTGTGAATCTGTGGTGACGAGTGGTCAACACAACTTGACCCCTCAGCGTCCACCAAGGGATGCAAGCCCTGCGGGACTTTTGTCCTTAGCAGAGGAAACTTTAACAGAGTTTTTATCAAAGGCAACTGGAACTGCTGTGGAGTGGGTCCAAATGCCTGGGATGAAGCCTGGTCCGGATTCCATTGGAATCATTGCAATTTCTCATGGTTGCTCAGGAGTAGCCTCGCGTGCTTGTGGCCTTGTGGGTCTAGAGCCTACAAGAGTTGCTGAAATTCTTAAAGATCGGCCATCATGGTTTCGTGACTGCCGAGCAGTGGATGTTCTCAATGTGATGTCCACTGGAAACGGTGGAACCATTGAATTGCTATACATGCAGCTCTATGCACCTACTACACTGGCACCAGCTCGTGACTTTTGGTTAATGCGGTATACATCTGTTATGGAAGATGGTAGCCTTGTGATATGTGAAAGATCCCTGAACAACACTCAGAATGGTCCTAGTATGCCACCGGTGCAGAGTTTTGTGAGAGCAGATATACTGCCGAGTGGATATCTGATACGACCCTGTGAAGGAGGTGGTTCAATCATTCACATAGTTGACCATATGGATTTAGAGCCATGGAGTGTGCCTGAAGTGTTGCGTCCACTGTATGAGTCATCAACATTGTTGTCGCAGAGGACAACTATGGCGGCATTACGTCACTTGAGACAAATTTCTCAAGAAATTTCACATCCTACTGTCTCTGGCTGGGGAAGAAGACCTGCTGCGTTGCGCGCACTTGGTCAAAGATTGAGCAAGGGTTTTAACGAGGCCGTTAACGGTTTTACTGATGAGGGATGGTCTATGCTTGAAAGTGATGGCGTAGATGATGTTACTATTCTTGTGAACTCATCACCCAGCAAATTGATGGGTGCAAACATCTCTTATGCCAATGGATTTCCATCGATGAGCAGCGCTGTGCTTTGTGCCAAGGCTTCTATGCTTTTACAGAACGTACCTCCACCCATTCTGCTAAGGTTCTTGCGGGAACACCGATCAGAATGGGCAGACAGTGGTATCGATGCTTATTCAGCTGCTGCAGTTAAAGCTGGTCCATGCAGCATACCAGTCACCCGAACTGGAAGTTTTGGTGGACAAATTATTCTTCCACTGGCTCACACAATTGAACATGAAGAGTTTATGGAGGTGATAAGACTTGAAAGCATTGGCCACTATCAAGATGACATGATAATGCCTAGTGACATCTTCCTTCTGCAACTTTGTAATGGAGTGGACGAGAATGCTATTGGAACATGTGCAGAGCTCATGTTTGCTCCTATTGATGCCTCATTTGCTGATGATGCTCCTCTCCTTCCCTCTGGTTTTCGTATCATTCCTCTGGATTCCAAGGCGGATGCATCTAGTCCAAACCGCACACTTGATCTTGCATCAACTCTTGAGGTTGGACCAGCTGGAAGTCGACCAACTGGGGATCATTCCAAGAATTCTGGTAGCGCAAAATCGGTTATGACAATAGCATTTCAATTTGCATTTGAGATTCATCTTCAAGAAAGCATAGCTGCTATGGCACGTCAATATGTCCGCAGTATCATATCATCTGTTCAGAGGGTTGCTTTGGCTCTCTCTCCATCTCGTATTGGTTCTCTCCCTGGTCTAAGATCTCCACCTGGCACACCTGAAGCCCAGACTCTTGCCCGTTGGATTTGCCAAAGTTATAGATTCTTCTTAGGCGTGGAGCTGCTCAAATCTGCTAGCGGAGGAAGTGAATCCATCCTGAAAGAAATTTGGGATCACTCAGATGCTCTGATGTGTTGCTCTATGAAGGCACTGCCAGTTTTCACATTCGCGAATGAGGCTGGACTTGACATGCTAGAAACCACCTTAGTAGCCCTTCAAGATATTAGTCTGGAAAAGATTTTCGATGACAATGGAAGAAAGGCTCTCTACTCTGAGCTTCCACAGATAATGCAGCAGGGTTTTGCATGTCTTCAAGGTGGTATCTGTTTGTCAAGTATGGGAAGGCCAATATCCTATGAGAGAGCAGTAGCTTGGAAAGTATTGAATGAAGAAGAAGATGCCCATTGTATATGTTTTATGTTTATCAATTGGTCTTTTGTCTGATATTATTTTGCACATCAATGTAGGAACTCATTAATGCAAACTTAGATCAACAACACTTCTTTTCTTTTTGAATCATACTTCATGGATCAACCTCCTTCCATTTTTTTACTTTCTTTTAATTATCTTTGGTTTTTAACTTACTCTTACTAGAAGCAATCTCACATTACATTTAGCTCACACATTCAGTGGGT
CDS Sequence
  • >Solyc08g066500.4.1
    CTTGAAGTGATGATGGCTGTGACATCAAGCTGTAAAGACAAATTTGGGATGGATAGTGGTAAATATGTGAGGTACACTCCTGAGCAGGTAGAGGCTTTGGAGAGGTTGTATCATGAATGTCCAAAGCCTAGTTCACTCCGTCGCCAGCAGTTGATTCGGGAGTGTCCAATTCTGTCGAATATCGAGCCAAAACAGATCAAAGTTTGGTTCCAGAACAGAAGATGTAGAGAGAAGCAACGTAAAGAATCATCGCGCCTTCAAGGCGTGAACAGAAAATTGACTGCAATGAATAAGCTGTTGATGGAAGAGAACGATAGGTTGCAGAAGCAAGTTTCACAGTTGGTGTATGAGAATAGCTTTTTCCGCCAACAGACTCAAACTGCAGCCTTAGCCACCACAGACAACAATAGTTGTGAATCTGTGGTGACGAGTGGTCAACACAACTTGACCCCTCAGCGTCCACCAAGGGATGCAAGCCCTGCGGGACTTTTGTCCTTAGCAGAGGAAACTTTAACAGAGTTTTTATCAAAGGCAACTGGAACTGCTGTGGAGTGGGTCCAAATGCCTGGGATGAAGCCTGGTCCGGATTCCATTGGAATCATTGCAATTTCTCATGGTTGCTCAGGAGTAGCCTCGCGTGCTTGTGGCCTTGTGGGTCTAGAGCCTACAAGAGTTGCTGAAATTCTTAAAGATCGGCCATCATGGTTTCGTGACTGCCGAGCAGTGGATGTTCTCAATGTGATGTCCACTGGAAACGGTGGAACCATTGAATTGCTATACATGCAGCTCTATGCACCTACTACACTGGCACCAGCTCGTGACTTTTGGTTAATGCGGTATACATCTGTTATGGAAGATGGTAGCCTTGTGATATGTGAAAGATCCCTGAACAACACTCAGAATGGTCCTAGTATGCCACCGGTGCAGAGTTTTGTGAGAGCAGATATACTGCCGAGTGGATATCTGATACGACCCTGTGAAGGAGGTGGTTCAATCATTCACATAGTTGACCATATGGATTTAGAGCCATGGAGTGTGCCTGAAGTGTTGCGTCCACTGTATGAGTCATCAACATTGTTGTCGCAGAGGACAACTATGGCGGCATTACGTCACTTGAGACAAATTTCTCAAGAAATTTCACATCCTACTGTCTCTGGCTGGGGAAGAAGACCTGCTGCGTTGCGCGCACTTGGTCAAAGATTGAGCAAGGGTTTTAACGAGGCCGTTAACGGTTTTACTGATGAGGGATGGTCTATGCTTGAAAGTGATGGCGTAGATGATGTTACTATTCTTGTGAACTCATCACCCAGCAAATTGATGGGTGCAAACATCTCTTATGCCAATGGATTTCCATCGATGAGCAGCGCTGTGCTTTGTGCCAAGGCTTCTATGCTTTTACAGAACGTACCTCCACCCATTCTGCTAAGGTTCTTGCGGGAACACCGATCAGAATGGGCAGACAGTGGTATCGATGCTTATTCAGCTGCTGCAGTTAAAGCTGGTCCATGCAGCATACCAGTCACCCGAACTGGAAGTTTTGGTGGACAAATTATTCTTCCACTGGCTCACACAATTGAACATGAAGAGTTTATGGAGGTGATAAGACTTGAAAGCATTGGCCACTATCAAGATGACATGATAATGCCTAGTGACATCTTCCTTCTGCAACTTTGTAATGGAGTGGACGAGAATGCTATTGGAACATGTGCAGAGCTCATGTTTGCTCCTATTGATGCCTCATTTGCTGATGATGCTCCTCTCCTTCCCTCTGGTTTTCGTATCATTCCTCTGGATTCCAAGGCGGATGCATCTAGTCCAAACCGCACACTTGATCTTGCATCAACTCTTGAGGTTGGACCAGCTGGAAGTCGACCAACTGGGGATCATTCCAAGAATTCTGGTAGCGCAAAATCGGTTATGACAATAGCATTTCAATTTGCATTTGAGATTCATCTTCAAGAAAGCATAGCTGCTATGGCACGTCAATATGTCCGCAGTATCATATCATCTGTTCAGAGGGTTGCTTTGGCTCTCTCTCCATCTCGTATTGGTTCTCTCCCTGGTCTAAGATCTCCACCTGGCACACCTGAAGCCCAGACTCTTGCCCGTTGGATTTGCCAAAGTTATAGATTCTTCTTAGGCGTGGAGCTGCTCAAATCTGCTAGCGGAGGAAGTGAATCCATCCTGAAAGAAATTTGGGATCACTCAGATGCTCTGATGTGTTGCTCTATGAAGGCACTGCCAGTTTTCACATTCGCGAATGAGGCTGGACTTGACATGCTAGAAACCACCTTAGTAGCCCTTCAAGATATTAGTCTGGAAAAGATTTTCGATGACAATGGAAGAAAGGCTCTCTACTCTGAGCTTCCACAGATAATGCAGCAGGGTTTTGCATGTCTTCAAGGTGGTATCTGTTTGTCAAGTATGGGAAGGCCAATATCCTATGAGAGAGCAGTAGCTTGGAAAGTATTGAATGAAGAAGAAGATGCCCATTGTATATGTTTTATGTTTATCAATTGGTCTTTTGTCTGA
Protein Sequence
  • >Solyc08g066500.4.1
    LEVMMAVTSSCKDKFGMDSGKYVRYTPEQVEALERLYHECPKPSSLRRQQLIRECPILSNIEPKQIKVWFQNRRCREKQRKESSRLQGVNRKLTAMNKLLMEENDRLQKQVSQLVYENSFFRQQTQTAALATTDNNSCESVVTSGQHNLTPQRPPRDASPAGLLSLAEETLTEFLSKATGTAVEWVQMPGMKPGPDSIGIIAISHGCSGVASRACGLVGLEPTRVAEILKDRPSWFRDCRAVDVLNVMSTGNGGTIELLYMQLYAPTTLAPARDFWLMRYTSVMEDGSLVICERSLNNTQNGPSMPPVQSFVRADILPSGYLIRPCEGGGSIIHIVDHMDLEPWSVPEVLRPLYESSTLLSQRTTMAALRHLRQISQEISHPTVSGWGRRPAALRALGQRLSKGFNEAVNGFTDEGWSMLESDGVDDVTILVNSSPSKLMGANISYANGFPSMSSAVLCAKASMLLQNVPPPILLRFLREHRSEWADSGIDAYSAAAVKAGPCSIPVTRTGSFGGQIILPLAHTIEHEEFMEVIRLESIGHYQDDMIMPSDIFLLQLCNGVDENAIGTCAELMFAPIDASFADDAPLLPSGFRIIPLDSKADASSPNRTLDLASTLEVGPAGSRPTGDHSKNSGSAKSVMTIAFQFAFEIHLQESIAAMARQYVRSIISSVQRVALALSPSRIGSLPGLRSPPGTPEAQTLARWICQSYRFFLGVELLKSASGGSESILKEIWDHSDALMCCSMKALPVFTFANEAGLDMLETTLVALQDISLEKIFDDNGRKALYSELPQIMQQGFACLQGGICLSSMGRPISYERAVAWKVLNEEEDAHCICFMFINWSFV*