Gene Details:

Functional Descriptions:

  • The transcription factor WRKY32 affects tomato fruit colour by regulating YELLOW FRUITED-TOMATO 1, a core component of ethylene signal transduction.
  • Based on bioinformatic analysis, yeast one hybrid assays and electrophoretic mobility shift assays, we report that transcription factor WRKY32 regulates tomato fruit colour formation.
  • In tomato fruits of WRKY32-RNAi generated lines, ethylene signalling was reduced, leading to a suppression in ethylene emission, a delay in chromoplast development, decreased carotenoid accumulation, and a yellow fruit phenotype.
  • Describe the characterization of WRKY32, a member of the WRKY TF family, and the mechanism by which it affects ripening and fruit colour formation through its interaction with the YFT1 promoter.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Solyc07g005650.4.1
    TTTACCTATTTTCTTCCTCTTAATTCATTTTTTTCACAAAAACACTCACTGTGTGTTCATCGGAATCCGCCATGGATGACGAAAACGAGAGCTCCAAATCAGCCGGACTCAAAAATTCAAATCTAGAAGAAGAAGAAGAAGATAAAATCAGCAACAAAATCGAAAATTCCTCCGTACACAACGACGAAAACGGTGACGACGATAGAGTAATTGACGGTAGCAGTGGAGCTGAGAGTTTACGAGAATCAAAGACGGGAGCTTTAATTTCAGAAACCCTAGCTCATCCTAATGCAGAATCATCCGTACAAATTGAAATTGATGGCCAATCCGAATTCGATTCAGAGTTTTCTCCCTCTTATCAACTTTCTGAAGTACCAGTGGAATATGAACTGCCCTCGTTTGGTTTCTCGGAAAAAATCAAGGATAGAATTAGTGTTACCAAACCTGGAACTTTGAATGCTCAAGCACGAACAGAACATCAACGACGTGTTCCTGATGCAGCATCCTCATTGGAGCTGTCCTCTTTATCTGTTGCACAGTCCATTTCATCAGTGCCAAGTGCAACTCTAGCAGAAAGACGATCAGCAGCAGCAGTAAATTGTAGTACAGGAGAAGTGGTTAAGCAGAGTTCTGACGCCCAGGTTCTAGCTCTTGTACCAGTTCTAAAGAGACCAACTCGCGATGGGTACAATTGGCGGAAGTATGGTCAAAAGCAAGTTAAAAGTCCTCAAGGTTCTCGAAGTTATTACCGATGCACTCATTCTGAGTGTTGTGCCAAGAAGATTGAGTGCTCTGATCACACTAATCGTGTTATGGAGATTATTTATAGAACTCAACACAATCATGATCCACCCCCAAGAGTAAATTGCCCAAGGGAAAGCAAGTCTGCATTATTGTCTTCACCTACCAATGGCAAAAGTATAATAGCTCATCCAAGGAGAAATTCTATTGAGACCGTAGTATCCCCCTTAAATGAAAATTTGCAAGAAAGTTTACCAATTGCTGAGACAGCAAATCAGGATTCAGGTGGGTCTGACACTGACACTGAAATCACTATTAGAGAGGAGCACCGTGACGAGGCTGGACAAAAGAAGAGGTCAAGGAAAAGTGACACAAGTTGTTTGGAATCTGTTTCTAAACCTGGAAAGAAACCCAAGCTTGTGGTGCATGCTGCTTGTGATGTAGGAATCTCAAGTGATGGCTACAGATGGCGAAAGTATGGACAAAAAATAGTGAAGGGAAATCCCCATCCAAGGAACTACTATCGCTGTACATCAGCTGGATGTCCCGTTCGAAAGCACATTGAGAGGGTTTTAGATACCACAAGTGCTTTAACAATAACATACAAGGGAGTACACGATCATGACATGCCAGTACCCAAAAAACGTCATGGTCCACCGAGTGCACCTCTTATTGCTGCTACTGCCCCTGCTTCCGTAACCACTATGCATGCTAACAAACCCGAACCCCTACAACATCAAAAATCGACCACACAATGGTCCGTGGACAAAGAAGGTGAGTTGACTGGTGAGAAATTGGATGTTGGAGGAGAAAAAGCAATGGAATCGGCTCGAACTCTATTGAGTATTGGATTTGAAATTAAGCCTTGCTAAAATTGCTTAGCTGCTCTGATTTTATTTTTTTCTGCATTTATTTAGTTTTCCTCCATAGGGCTTTCTTGTAGAAGTTTAGAAGAGTACACCTTTTTTTTTTATAGTCCTCCAATATATAGATGGTCTATACTCAGTTATGTACATTATTGTGGATCAAAATAAGTAACATAGAACTTAGAATAACATATTGTGATGTTTGTCAATTTGCATCTGTTTC
CDS Sequence
  • >Solyc07g005650.4.1
    ATGGATGACGAAAACGAGAGCTCCAAATCAGCCGGACTCAAAAATTCAAATCTAGAAGAAGAAGAAGAAGATAAAATCAGCAACAAAATCGAAAATTCCTCCGTACACAACGACGAAAACGGTGACGACGATAGAGTAATTGACGGTAGCAGTGGAGCTGAGAGTTTACGAGAATCAAAGACGGGAGCTTTAATTTCAGAAACCCTAGCTCATCCTAATGCAGAATCATCCGTACAAATTGAAATTGATGGCCAATCCGAATTCGATTCAGAGTTTTCTCCCTCTTATCAACTTTCTGAAGTACCAGTGGAATATGAACTGCCCTCGTTTGGTTTCTCGGAAAAAATCAAGGATAGAATTAGTGTTACCAAACCTGGAACTTTGAATGCTCAAGCACGAACAGAACATCAACGACGTGTTCCTGATGCAGCATCCTCATTGGAGCTGTCCTCTTTATCTGTTGCACAGTCCATTTCATCAGTGCCAAGTGCAACTCTAGCAGAAAGACGATCAGCAGCAGCAGTAAATTGTAGTACAGGAGAAGTGGTTAAGCAGAGTTCTGACGCCCAGGTTCTAGCTCTTGTACCAGTTCTAAAGAGACCAACTCGCGATGGGTACAATTGGCGGAAGTATGGTCAAAAGCAAGTTAAAAGTCCTCAAGGTTCTCGAAGTTATTACCGATGCACTCATTCTGAGTGTTGTGCCAAGAAGATTGAGTGCTCTGATCACACTAATCGTGTTATGGAGATTATTTATAGAACTCAACACAATCATGATCCACCCCCAAGAGTAAATTGCCCAAGGGAAAGCAAGTCTGCATTATTGTCTTCACCTACCAATGGCAAAAGTATAATAGCTCATCCAAGGAGAAATTCTATTGAGACCGTAGTATCCCCCTTAAATGAAAATTTGCAAGAAAGTTTACCAATTGCTGAGACAGCAAATCAGGATTCAGGTGGGTCTGACACTGACACTGAAATCACTATTAGAGAGGAGCACCGTGACGAGGCTGGACAAAAGAAGAGGTCAAGGAAAAGTGACACAAGTTGTTTGGAATCTGTTTCTAAACCTGGAAAGAAACCCAAGCTTGTGGTGCATGCTGCTTGTGATGTAGGAATCTCAAGTGATGGCTACAGATGGCGAAAGTATGGACAAAAAATAGTGAAGGGAAATCCCCATCCAAGGAACTACTATCGCTGTACATCAGCTGGATGTCCCGTTCGAAAGCACATTGAGAGGGTTTTAGATACCACAAGTGCTTTAACAATAACATACAAGGGAGTACACGATCATGACATGCCAGTACCCAAAAAACGTCATGGTCCACCGAGTGCACCTCTTATTGCTGCTACTGCCCCTGCTTCCGTAACCACTATGCATGCTAACAAACCCGAACCCCTACAACATCAAAAATCGACCACACAATGGTCCGTGGACAAAGAAGGTGAGTTGACTGGTGAGAAATTGGATGTTGGAGGAGAAAAAGCAATGGAATCGGCTCGAACTCTATTGAGTATTGGATTTGAAATTAAGCCTTGCTAA
Protein Sequence
  • >Solyc07g005650.4.1
    MDDENESSKSAGLKNSNLEEEEEDKISNKIENSSVHNDENGDDDRVIDGSSGAESLRESKTGALISETLAHPNAESSVQIEIDGQSEFDSEFSPSYQLSEVPVEYELPSFGFSEKIKDRISVTKPGTLNAQARTEHQRRVPDAASSLELSSLSVAQSISSVPSATLAERRSAAAVNCSTGEVVKQSSDAQVLALVPVLKRPTRDGYNWRKYGQKQVKSPQGSRSYYRCTHSECCAKKIECSDHTNRVMEIIYRTQHNHDPPPRVNCPRESKSALLSSPTNGKSIIAHPRRNSIETVVSPLNENLQESLPIAETANQDSGGSDTDTEITIREEHRDEAGQKKRSRKSDTSCLESVSKPGKKPKLVVHAACDVGISSDGYRWRKYGQKIVKGNPHPRNYYRCTSAGCPVRKHIERVLDTTSALTITYKGVHDHDMPVPKKRHGPPSAPLIAATAPASVTTMHANKPEPLQHQKSTTQWSVDKEGELTGEKLDVGGEKAMESARTLLSIGFEIKPC*