Gene Details:

Functional Descriptions:

  • Silencing of the tomato phosphatidylinositol-phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea.
  • CRISPR/Cas9-mediated phospholipase C 2 (SlPLC2) knock-out tomato plants are more resistant to Botrytis cinerea
  • SlPLC2-knock-out plants showed an enhanced resistance with smaller necrotic areas and reduced pathogen proliferation. Thus, we obtained SlPLC2 loss-of-function tomato lines more resistant to B. cinerea by means of CRISPR/Cas9 genome editing technology.
  • SlPLC2 silenced plants developed smaller necrotic lesions concomitantly with less proliferation of the fungus.
  • This implies that SlPLC2 participates in plant susceptibility to B. cinerea.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Solyc06g051620.3.1
    AAGGCATAGTTGAACAACTTCAACCGTATGTTTCAAGGCCTTTATTTTATTTGTTTGTATAGCAAGTTGATGTATTTACTTATAACCTTTGTTATGAAATTCAAACAACGTTAGTTACATATGATATTTTAATTTTATAATATACCGACTTCAAATCATGTAGTTTCACTTTCACACGTTACATTAGACCAAAAAGGAAGAAGCAGAGAAACTCCATGTTTAAAATGTCTCAATCAGTAAATCGGATAAGCCGACTAAAACATCAGACTAGCCTTGTGGCCCTACCATAATTAATCTCCTTTGAAATTTTATTAAACAGATGGCAAAAAATACAATTCTGTACTATTTGTTAAATGTTGAATGGTTGCCTATAGCCTATACTTAACCCAATTCATTTTGTTTAACAGCACATTTTTTCTTTTAGTAATATAAAAGAGTACTACTCAACGCATAACGAAAAAGTGTAAATTTGTTAACACGACGATCGGATCCTGCCTGAGCAAAAACGAAATCGTTTTCCGTTAGGGTGACAATGTCGAAACAAACGTACAAAGTCGGATTTTTTTTCCGGCGGCAGTTCACCATGGCTGCCGCGGAAGCTCCGGCGGACATTAAGAGCCTATTTAAACGTTATTCCGACGACAGCGGAGTTATGAGCGTCCAGAATCTTCATAGTTTCTTAATTGAGATCCAGAAGGAGAAAAATGTTAGCTTAGAGAACGCAGAAGCCATTATCAATAATCACGGCGGCGACTCCAAGCAGAAAGGCCTCCAGCTCGATGGCTTCTTCAAGTTTCTCTTTTCTGATGTTAATCCTCCTCTCGATCCTAAACTTGGGATTCACCATGATATGACTGCACCTCTGTCTCATTACTACATATACACAGGCCATAATTCATATTTAACTGGGAATCAACTGAGTAGTGATTGCAGTGATGTTCCCATAATACAAGCTCTTCAGAGAAGTGTTAGAGTAATTGAATTAGATATTTGGCCAAATTCAGACAAAGATGATATAGAAGTTCTACATGGAAGGACACTGACTGCTCCAGTGACGCTAATCAAATGTTTGAGGTCTATCAAGGAGCATGCCTTTTGTGCATCGGAGTATCCTCTTGTAATAACACTTGAAGATCATCTCACGCCAGATCTTCAGGAAAAAGTAGCTGAGATGATCACTCAAACATTTGGAGAGATGCTGTTTTCTCCTTCAGAAAGTTTGAAGGAGCTTCCTTCTCCGGAGTCACTGAGAAAGCGTGTGATGATATCAACTAAGCCACCAAAAGAATACTTAAAATCCAAGGAAGTTAAGGAAAAAGATGACACGAAGAAAGAAGCTGAGGCAGCTTCAGAATACAAGCGCCTAATTGCCATTCATGCTGGAAAGGGGAAAGGAGGACTATCTGATTGGTTGAGGGTTGACCTCAATAAAGTAAGACGGCTTAGTCTCAGTGAACCAGAACTTGAAAAAGCTGTAGATACTCATGCAAAAGAAATTATCAGGTTCACTCAGCATAACTTGCTGAGAATATACCCAAAGGGCATTCGAGTTGACTCGTCCAATTATGATCCTTTTGTTGGTTGGATGCACGGAGCTCAAATGGTAGCATTTAATATGCAGGGTTATGGAAGATCACTTTGGTTGATGCATGGTATGTTCAGGGCTAATGGTGGCTGTGGATATGTTAAGAAACCTGATCTACTATTGAAAGCTGGTCCCGACAATGAGGTCTTCGATCCTACAGCAAATTTGCCTGTCAAAACTACACTAAAGGTGACTGTATATATGGGTGATGGGTGGGACAAAGACTTCGATCAGACAGACTTTGACACGTACTCACCTCCAGATTTCTATGCAAAGCTAGGAATTGCTGGAGTTCCTGCTGATGAAGTAAAGAAGAGAACAGAGACGATAGACGACAATTGGATACCAAGCTGGAATGAGCAGTTTGAGTTTCCACTAACCGTTCCTGAGTTAGCTCTACTTCGTATCAAAGTTCTTGATTATAATTTGTCCGACAAGGATGAGTTTGCTGGCCAAACATGTCTACCTGTGGCAGAGCTGCGACAAGGTATCCGAGCAGTCCCACTCTATGATCGGAAAGGAGAGAAGTACAGTTCTGTGAAGCTTCTCATGCATTTCGAGTTTAAATAACTTTGTAGATCTTGAAAAGGGAGCTTTGCTAGTTTCATCTATGTGAAGCGTAGCAAAGTTTATAAACTATATACAAGTTACAACCACATGCAAAGTTGTAAGATATATATGTACCATCAGATAGAGTACACACAGACTGATGGAAATAAGGTTAGTAAACAAAAAACAATAAAATTACTATGGGAGAAAAATATGGGAAGATTGAAGATAGAAAATAAGATTGGTTAAATTTGAGAAATAACTTCCCAAATAACCTCATGTATATGATGCCCCTTACCTTAATCCATATCAAATTGCTTATTAAGAGCATACTCTTGAGCCAAGCTTTTAAGTCTTTCGAAAGCTCTAC
CDS Sequence
  • >Solyc06g051620.3.1
    ATGTCGAAACAAACGTACAAAGTCGGATTTTTTTTCCGGCGGCAGTTCACCATGGCTGCCGCGGAAGCTCCGGCGGACATTAAGAGCCTATTTAAACGTTATTCCGACGACAGCGGAGTTATGAGCGTCCAGAATCTTCATAGTTTCTTAATTGAGATCCAGAAGGAGAAAAATGTTAGCTTAGAGAACGCAGAAGCCATTATCAATAATCACGGCGGCGACTCCAAGCAGAAAGGCCTCCAGCTCGATGGCTTCTTCAAGTTTCTCTTTTCTGATGTTAATCCTCCTCTCGATCCTAAACTTGGGATTCACCATGATATGACTGCACCTCTGTCTCATTACTACATATACACAGGCCATAATTCATATTTAACTGGGAATCAACTGAGTAGTGATTGCAGTGATGTTCCCATAATACAAGCTCTTCAGAGAAGTGTTAGAGTAATTGAATTAGATATTTGGCCAAATTCAGACAAAGATGATATAGAAGTTCTACATGGAAGGACACTGACTGCTCCAGTGACGCTAATCAAATGTTTGAGGTCTATCAAGGAGCATGCCTTTTGTGCATCGGAGTATCCTCTTGTAATAACACTTGAAGATCATCTCACGCCAGATCTTCAGGAAAAAGTAGCTGAGATGATCACTCAAACATTTGGAGAGATGCTGTTTTCTCCTTCAGAAAGTTTGAAGGAGCTTCCTTCTCCGGAGTCACTGAGAAAGCGTGTGATGATATCAACTAAGCCACCAAAAGAATACTTAAAATCCAAGGAAGTTAAGGAAAAAGATGACACGAAGAAAGAAGCTGAGGCAGCTTCAGAATACAAGCGCCTAATTGCCATTCATGCTGGAAAGGGGAAAGGAGGACTATCTGATTGGTTGAGGGTTGACCTCAATAAAGTAAGACGGCTTAGTCTCAGTGAACCAGAACTTGAAAAAGCTGTAGATACTCATGCAAAAGAAATTATCAGGTTCACTCAGCATAACTTGCTGAGAATATACCCAAAGGGCATTCGAGTTGACTCGTCCAATTATGATCCTTTTGTTGGTTGGATGCACGGAGCTCAAATGGTAGCATTTAATATGCAGGGTTATGGAAGATCACTTTGGTTGATGCATGGTATGTTCAGGGCTAATGGTGGCTGTGGATATGTTAAGAAACCTGATCTACTATTGAAAGCTGGTCCCGACAATGAGGTCTTCGATCCTACAGCAAATTTGCCTGTCAAAACTACACTAAAGGTGACTGTATATATGGGTGATGGGTGGGACAAAGACTTCGATCAGACAGACTTTGACACGTACTCACCTCCAGATTTCTATGCAAAGCTAGGAATTGCTGGAGTTCCTGCTGATGAAGTAAAGAAGAGAACAGAGACGATAGACGACAATTGGATACCAAGCTGGAATGAGCAGTTTGAGTTTCCACTAACCGTTCCTGAGTTAGCTCTACTTCGTATCAAAGTTCTTGATTATAATTTGTCCGACAAGGATGAGTTTGCTGGCCAAACATGTCTACCTGTGGCAGAGCTGCGACAAGGTATCCGAGCAGTCCCACTCTATGATCGGAAAGGAGAGAAGTACAGTTCTGTGAAGCTTCTCATGCATTTCGAGTTTAAATAA
Protein Sequence
  • >Solyc06g051620.3.1
    MSKQTYKVGFFFRRQFTMAAAEAPADIKSLFKRYSDDSGVMSVQNLHSFLIEIQKEKNVSLENAEAIINNHGGDSKQKGLQLDGFFKFLFSDVNPPLDPKLGIHHDMTAPLSHYYIYTGHNSYLTGNQLSSDCSDVPIIQALQRSVRVIELDIWPNSDKDDIEVLHGRTLTAPVTLIKCLRSIKEHAFCASEYPLVITLEDHLTPDLQEKVAEMITQTFGEMLFSPSESLKELPSPESLRKRVMISTKPPKEYLKSKEVKEKDDTKKEAEAASEYKRLIAIHAGKGKGGLSDWLRVDLNKVRRLSLSEPELEKAVDTHAKEIIRFTQHNLLRIYPKGIRVDSSNYDPFVGWMHGAQMVAFNMQGYGRSLWLMHGMFRANGGCGYVKKPDLLLKAGPDNEVFDPTANLPVKTTLKVTVYMGDGWDKDFDQTDFDTYSPPDFYAKLGIAGVPADEVKKRTETIDDNWIPSWNEQFEFPLTVPELALLRIKVLDYNLSDKDEFAGQTCLPVAELRQGIRAVPLYDRKGEKYSSVKLLMHFEFK*