Gene Details:

Functional Descriptions:

  • SlIDA expression during anther development and pollen tube elongation.
  • Tomato SlIDA has a critical role in tomato fertilization by modifying reactive oxygen species homeostasis.
  • We generated tomato knockout lines (CR-SlIDA) of an IDA homolog (SlIDA), which is expressed in the tapetum, septum and pollen tube, and observed a severe defect in male gametes.
  • Liquid chromatography-tandem mass spectrometry identified mature SlIDA as a 14-mer EPIP peptide, which was shown to be secreted, and a complementation experiment showed that application of a synthetic 14-mer EPIP peptide rescued the CR-SlIDA defect and enhanced the ROS signal.
  • Collectively, our results revealed that SlIDA plays an essential role in pollen development and pollen tube elongation by modulating ROS homeostasis.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Solyc05g010000.1.1
    ATGGCTTTCTCTTTTTCTTCTTCAAAAACCCTTTATTTATCAAGCAAATTAACTTGTTTGATACTTGTTATTTCTCTACTTTTTAATTATGGTCATATTGTTGAAGCATCAAGATTTGGGAGAATTATGATGGTAGAGGAAAATTCAAGAATATTTTCATCACAACATATGAAGGTATACAAAAAAGAAAATGCATACAAAGTTGATAATTTATTATTTACTATGTTACCAAAAGGGATTCCAATTCCTCCTTCTGCTCCATCAAAGAGACATAATGCTATTGAAGACTCTACACCTCAAAATTGA
CDS Sequence
  • >Solyc05g010000.1.1
    ATGGCTTTCTCTTTTTCTTCTTCAAAAACCCTTTATTTATCAAGCAAATTAACTTGTTTGATACTTGTTATTTCTCTACTTTTTAATTATGGTCATATTGTTGAAGCATCAAGATTTGGGAGAATTATGATGGTAGAGGAAAATTCAAGAATATTTTCATCACAACATATGAAGGTATACAAAAAAGAAAATGCATACAAAGTTGATAATTTATTATTTACTATGTTACCAAAAGGGATTCCAATTCCTCCTTCTGCTCCATCAAAGAGACATAATGCTATTGAAGACTCTACACCTCAAAATTGA
Protein Sequence
  • >Solyc05g010000.1.1
    MAFSFSSSKTLYLSSKLTCLILVISLLFNYGHIVEASRFGRIMMVEENSRIFSSQHMKVYKKENAYKVDNLLFTMLPKGIPIPPSAPSKRHNAIEDSTPQN*