Gene Details:
- Gene ID: Solyc03g013340
- Gene Symbol: SlLsi1
- Gene Name: silicon influx transporter LSI1
- Genome: Tomato Genome version SL4.0
- Species: Solanum lycopersicum
Functional Descriptions:
- Here, we isolated homologous genes of rice Si influx (SlLsi1) and efflux (SlLsi2) transporter genes in tomato (Solanum lycopersicum L.) and functionally characterized these genes.
- SlLsi1 showed transport activity for Si when expressed in both rice lsi1 mutant and Xenopus laevis oocytes. SlLsi1 was constitutively expressed in the roots. Immunostaining showed that SlLsi1 was localized at the plasma membrane of both root tip and basal region without polarity.
- Overexpression of SlLsi1 in tomato increased Si concentration in the roots and root cell sap but did not alter the Si concentration in the shoots.
- Our results suggest that the low Si accumulation in tomato is attributed to the lack of functional Si efflux transporter Lsi2 required for active Si uptake although SlLsi1 is functional.
Function-related keywords:
Literature:
- Tomato roots have a functional silicon influx transporter but not a functional silicon efflux transporter. DOI: 10.1111/pce.13679 ; PMID: 31724184
Related News:
Gene Resources:
Orthologs:
Sequences:
cDNA Sequence
- >Solyc03g013340.3.1
AGAAGTAAATTCCCTAGCTAGCTGGCTAGCTCTTTCAATTCGTCTCCTTTAGAAACATTTTCAATTATATATTTGTGTATACTTCATTAAGTATCTGTGAAGAATGGAGAGTGAAGGAGGGAACTGTAGCAAAAGCATAAACCAAAATGAATTGGTATTGAAGGAAGATCCGAAATCAAACTTTTTTCAAAAGTATTATCGATCTGGTATTATAAAGAAGGTGATAGCAGAGATAATAGCGACGTACCTATTAGTGTTTGTGACATGTGGTGCAGCAAGTCTTAGTTGGAGCGATGAACACAAAGTATCAAAGCTTGGAGCGTCCGTAGCTGGTGGACTCATTGTAACTGTCATGATATATGCTGTTGGACATATATCTGGTGCTCATATGAACCCTGCTGTTACCTTTGCTTTTGCCGCCGTTAGACATTTCCCATGGACACAGGTGCCAGTGTATGCAGCAGCACAAGTTACAGGAGCAATATCTGCTGCATTCACGTTACGTGTATTACTTCATCCAGTAACTAAAAATGTGGGAACTACAACTCCCTCAGGCTCAGATATTCAAGCTTTAATCATGGAAATTGTCGTCACATTCTCTATGATGTTCATCACCTCTGCTGTCGCCACTGATACCAAAGCTATTGGTGAGCTGGCGGGAATTGCAGTTGGTTCGGCCGTATGTATTACTTCTATTTTGGCGGGGCCAGTATCAGGAGGATCAATGAACCCAGCTAGAAGCATTGGACCAGCAATGGCTAGCAATGATTACAGAGCAATATGGGTTTATATCATTGGACCCGTTTGTGGAACATTACTTGGAGCATGGTCCTATAATTTCATCAAGGTTAATGATAAACCAGTACAAGCAATAGTACCCGGACAATCCTTCTCATTTAAACTTAGAAGAATGAAGAGCAATAATCACGATGAAGAACAATGTGTTACCCTCTAAATGATTCTACTTCTTCCAATCTCATTTCACCCCTCTCTATGAA
CDS Sequence
- >Solyc03g013340.3.1
ATGGAGAGTGAAGGAGGGAACTGTAGCAAAAGCATAAACCAAAATGAATTGGTATTGAAGGAAGATCCGAAATCAAACTTTTTTCAAAAGTATTATCGATCTGGTATTATAAAGAAGGTGATAGCAGAGATAATAGCGACGTACCTATTAGTGTTTGTGACATGTGGTGCAGCAAGTCTTAGTTGGAGCGATGAACACAAAGTATCAAAGCTTGGAGCGTCCGTAGCTGGTGGACTCATTGTAACTGTCATGATATATGCTGTTGGACATATATCTGGTGCTCATATGAACCCTGCTGTTACCTTTGCTTTTGCCGCCGTTAGACATTTCCCATGGACACAGGTGCCAGTGTATGCAGCAGCACAAGTTACAGGAGCAATATCTGCTGCATTCACGTTACGTGTATTACTTCATCCAGTAACTAAAAATGTGGGAACTACAACTCCCTCAGGCTCAGATATTCAAGCTTTAATCATGGAAATTGTCGTCACATTCTCTATGATGTTCATCACCTCTGCTGTCGCCACTGATACCAAAGCTATTGGTGAGCTGGCGGGAATTGCAGTTGGTTCGGCCGTATGTATTACTTCTATTTTGGCGGGGCCAGTATCAGGAGGATCAATGAACCCAGCTAGAAGCATTGGACCAGCAATGGCTAGCAATGATTACAGAGCAATATGGGTTTATATCATTGGACCCGTTTGTGGAACATTACTTGGAGCATGGTCCTATAATTTCATCAAGGTTAATGATAAACCAGTACAAGCAATAGTACCCGGACAATCCTTCTCATTTAAACTTAGAAGAATGAAGAGCAATAATCACGATGAAGAACAATGTGTTACCCTCTAA
Protein Sequence
- >Solyc03g013340.3.1
MESEGGNCSKSINQNELVLKEDPKSNFFQKYYRSGIIKKVIAEIIATYLLVFVTCGAASLSWSDEHKVSKLGASVAGGLIVTVMIYAVGHISGAHMNPAVTFAFAAVRHFPWTQVPVYAAAQVTGAISAAFTLRVLLHPVTKNVGTTTPSGSDIQALIMEIVVTFSMMFITSAVATDTKAIGELAGIAVGSAVCITSILAGPVSGGSMNPARSIGPAMASNDYRAIWVYIIGPVCGTLLGAWSYNFIKVNDKPVQAIVPGQSFSFKLRRMKSNNHDEEQCVTL*