Information report for SlOSCA4.1
Gene Details
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Functional Descriptions
- A CRISPR/Cas9 SlOSCA4.1 mutant was resistant to PepMV, but not to tobacco mosaic virus or potato virus X. Inoculation of mutant and wild type tomato protoplasts showed that resistance was expressed in single cells, suggesting a role for SlOSCA4.1 in early viral function(s).
- We propose that SlOSCA4.1 contributes to the correct regulation of the Ca2+ homeostasis necessary for optimal PepMV infection.
- Solyc02g083430 encodes SlOSCA4.1, a member of the hyperosmolality-gated calcium-permeable channel 1 (OSCA) family.
- Our RNA-Seq analysis suggested the involvement of SlOSCA4.1 in the stress response, perhaps through its transmembrane Ca2+ transport function. Ca2+ signalling is central to both pattern- and effector-triggered immunity, with the generation of characteristic cytoplasmic Ca2+ waves in response to potential cellular pathogens.
Functional Keywords
- resistance , stress , immunity , homeostasis , calcium , viral-resistance , resistant , stress-response , protoplasts , immunity-signalling , virus , pepmv
Literature and News
Gene Resources
- NCBI ID: 101243949
- UniProt accessions: A0A3Q7F8R6
- AlphaFoldDB: A0A3Q7F8R6
- EnsemblPlants: Solyc02g083430.1.1
- Gramene: Solyc02g083430.1.1
- KEGG: sly:101243949
- GO: GO:0005227 , GO:0005886 , GO:0006623
- OMA: EYFQHRY
- InterPro: IPR003864 , IPR027815 , IPR032880 , IPR045122
- PANTHER: PTHR13018 , PTHR13018:SF114
- OrthoDB: A0A3Q7F8R6
- Pfam: PF02714 , PF13967 , PF14703
- SMR: A0A3Q7F8R6
- STRING: 4081.A0A3Q7F8R6
Sequences
cDNA Sequence
- >Solyc02g083430.1.1
ATGATCCAATCCAGCTTCTCTGCAGACTCACCTTCCATGGCAGCCAATTCCACTTTCTCTCCTCCGCCGGCCGCCGGTGACGGAGACTTCAATTACGACGTCGCTTGGTATGGTAACATCCAGTACCTCCTCAATATCTCCGCCATTGGAGCTTTGACTTGCCTTCTTATTTTCATCTTCGGGAAGCTTCGAAGCGACCACCGTCGCATGCCCGGTCCCACTGCCATTGTCTCCAAGCTCTTAGCTGCCTGGCACGCCACTGGTGTTGAAATCGCCCGCCACTGCGGGGCTGACGCTGCTCAATATCTCCTTATTGAGGGCGGCAGCTCTGCCCTGCTATTATTCCTCGCCCTTCTTTCTCTTGCTGTAATGCTGCCGTTGAATATATATGCTGGTAAGGCTCCTATGGCTGATCAGTTTTCAAAGACTACAATAAACCATATAGAAAAAGGTTCTCCATTACTCTGGATTCACTTTATATTTGTTGTTATTGTTGTTGTTTTGGTACATTATGGTATAAGTGAAATACAAGAAAGGTTGAAAATTACTAGACTTAGAGACGGCTATGGAAATCCGAGTAATTCTGGTACAAATGTCAGTGCAATTTTTTCCATTATGGTGCAGGGTGTACCTAAGACCTTAGGTTTTGATAAGACACCTTTAGTGGAGTATTTTCAGCATAAATATCCGGGGAAGGTGTATAGAGTAGTTGTCCCTATGGATTTGTGTGCTCTGGATGATTTAGCTACAGAGTTGGTGAAGGTTCGGGAAGATATCTCTAAACTAGTCTCAAGAATTGAGTTACGGGGTTATTTGAATGAGGGTGAGGAAGACGAGTATAATAATGATAGTGTGAACGGGCGGGGCTTGTTAGAACGACTGTGCTTTTTGTGGAGAAAGGCTAAGGATACATGGTATCATGTTGTGGATCAATTAGGTTTCTCAGATGAAGAGAGATTAAGAAAATTGCAAGAGTTGAGAGCTGATTTGGAGATGGAAATGGCATCTTATAAAGAAGGGAGGGCAAGAGGTGCTGGTGTAGCTTTTGTGGTATTTAAGGACGTATTCACAGCTAATAAGGCTGTCCAGGACCTCCGGAATGAGAAGAGGAGGCGATATGGTCGATTCTTCTCAGTCATTGAGTTGCAACTACAGAGGAACCAGTGGAAAGTGGAGAGAGCTCCTTTAGCTACTGACATATACTGGAACCACCTGGGATCAACAAAGTTCTCCTTAAAGCTGCGCAGAGTGTTGGTGAACACATGCCTATTGTTGATGTTGCTATTCTGCAGTTCTCCACTAGCTGTGATTAGTGCTATTCAAAGTGCAGGGAGAATAATCAATGCTGAAGCTATGGATCATGCTCAGATGTGGCTGAACTGGGTGCAGGGCTCGAGCTGGCTAGCAACAATAATATTTCAATTTTTGCCCAATGTTCTGATTTTTGTGAGCATGTACATTGTTGTCCCTTCAGTTCTTTCTTATCTTTCTAAATTTGAACAACATCTTACTGTATCTGGTGAGCAAAGGGCTGAGCTACTGAAAATGGTTTGCTTCTTTCTGGTAAATCTCATTCTGCTTAGGGCTCTGGTCGAATCTACTCTTGAGGGTGCTCTCTTAAGTATGGGTCGGTGTTATTTGGATGGAGAAGATTGCAAAAAGATCGAGCAGTACATGACTGCTTCCTTTTTGACAAGGACATGCCTCTCGTCTCTTGCATTTTTAATTACAAGCAGTTTTTTGGGTATATCTTTTGATTTATTAGCTCCAATTCCTTGGATTAAGAAGAAGCTTCAAAAGTTCCGTAAAAATGACATGCTTCAGTTGGTACCAGAACGGAGTGAGGAGTACCCATTGGAAAATCAAGACATTGATAGTTTGGAGAGGCCTCTGATTCATGAAAGGAGTTCAACTGTGATTGCTGACAACAATGGATTTTTACACGATGCCTCTCCAAATGAAATTGATTTCCCTGGACAAGATTTGTCTGAATACCCTCCAGTCAGCCGAACCTCACCAGTTCCAAAGCCGAAGTTTGATTTTGCACAGTATTATGCTTTCAATCTGACAATATTTGCCCTAACCCTGATCTATTGTTCGTTTGCTCCTCTGGTGGTTCCTGTTGGTGCAGTTTACTTTGGGTACCGGTATTTAGTTGACAAGTACAACTTCCTGTTTGTATACAGAGTGCGAGGTTTCCCTGCTGGTAATGATGGGAGGTTGATGGATACTGTATTATCTATCATGAGGTTTTGTGTTGACTTGTTCCTCCTTTCAATGCTACTTTTCTTTTCTGTACGAGGAGACTCAACTAAGCTTCAAGCCATATTCACACTTGGATTGTTAGTGGTGTATAAACTCTTGCCCTCTGATAAGGATTCTTTTCAGCCAGCGTTATTACAAGGCATACAGACTATTGACAACATTGTCGAAGGGCCAACTGATTATGAGGTTTTCTCACAACCTACATTTGATTGGGATACGTATAATTCATGA
CDS Sequence
- >Solyc02g083430.1.1
ATGATCCAATCCAGCTTCTCTGCAGACTCACCTTCCATGGCAGCCAATTCCACTTTCTCTCCTCCGCCGGCCGCCGGTGACGGAGACTTCAATTACGACGTCGCTTGGTATGGTAACATCCAGTACCTCCTCAATATCTCCGCCATTGGAGCTTTGACTTGCCTTCTTATTTTCATCTTCGGGAAGCTTCGAAGCGACCACCGTCGCATGCCCGGTCCCACTGCCATTGTCTCCAAGCTCTTAGCTGCCTGGCACGCCACTGGTGTTGAAATCGCCCGCCACTGCGGGGCTGACGCTGCTCAATATCTCCTTATTGAGGGCGGCAGCTCTGCCCTGCTATTATTCCTCGCCCTTCTTTCTCTTGCTGTAATGCTGCCGTTGAATATATATGCTGGTAAGGCTCCTATGGCTGATCAGTTTTCAAAGACTACAATAAACCATATAGAAAAAGGTTCTCCATTACTCTGGATTCACTTTATATTTGTTGTTATTGTTGTTGTTTTGGTACATTATGGTATAAGTGAAATACAAGAAAGGTTGAAAATTACTAGACTTAGAGACGGCTATGGAAATCCGAGTAATTCTGGTACAAATGTCAGTGCAATTTTTTCCATTATGGTGCAGGGTGTACCTAAGACCTTAGGTTTTGATAAGACACCTTTAGTGGAGTATTTTCAGCATAAATATCCGGGGAAGGTGTATAGAGTAGTTGTCCCTATGGATTTGTGTGCTCTGGATGATTTAGCTACAGAGTTGGTGAAGGTTCGGGAAGATATCTCTAAACTAGTCTCAAGAATTGAGTTACGGGGTTATTTGAATGAGGGTGAGGAAGACGAGTATAATAATGATAGTGTGAACGGGCGGGGCTTGTTAGAACGACTGTGCTTTTTGTGGAGAAAGGCTAAGGATACATGGTATCATGTTGTGGATCAATTAGGTTTCTCAGATGAAGAGAGATTAAGAAAATTGCAAGAGTTGAGAGCTGATTTGGAGATGGAAATGGCATCTTATAAAGAAGGGAGGGCAAGAGGTGCTGGTGTAGCTTTTGTGGTATTTAAGGACGTATTCACAGCTAATAAGGCTGTCCAGGACCTCCGGAATGAGAAGAGGAGGCGATATGGTCGATTCTTCTCAGTCATTGAGTTGCAACTACAGAGGAACCAGTGGAAAGTGGAGAGAGCTCCTTTAGCTACTGACATATACTGGAACCACCTGGGATCAACAAAGTTCTCCTTAAAGCTGCGCAGAGTGTTGGTGAACACATGCCTATTGTTGATGTTGCTATTCTGCAGTTCTCCACTAGCTGTGATTAGTGCTATTCAAAGTGCAGGGAGAATAATCAATGCTGAAGCTATGGATCATGCTCAGATGTGGCTGAACTGGGTGCAGGGCTCGAGCTGGCTAGCAACAATAATATTTCAATTTTTGCCCAATGTTCTGATTTTTGTGAGCATGTACATTGTTGTCCCTTCAGTTCTTTCTTATCTTTCTAAATTTGAACAACATCTTACTGTATCTGGTGAGCAAAGGGCTGAGCTACTGAAAATGGTTTGCTTCTTTCTGGTAAATCTCATTCTGCTTAGGGCTCTGGTCGAATCTACTCTTGAGGGTGCTCTCTTAAGTATGGGTCGGTGTTATTTGGATGGAGAAGATTGCAAAAAGATCGAGCAGTACATGACTGCTTCCTTTTTGACAAGGACATGCCTCTCGTCTCTTGCATTTTTAATTACAAGCAGTTTTTTGGGTATATCTTTTGATTTATTAGCTCCAATTCCTTGGATTAAGAAGAAGCTTCAAAAGTTCCGTAAAAATGACATGCTTCAGTTGGTACCAGAACGGAGTGAGGAGTACCCATTGGAAAATCAAGACATTGATAGTTTGGAGAGGCCTCTGATTCATGAAAGGAGTTCAACTGTGATTGCTGACAACAATGGATTTTTACACGATGCCTCTCCAAATGAAATTGATTTCCCTGGACAAGATTTGTCTGAATACCCTCCAGTCAGCCGAACCTCACCAGTTCCAAAGCCGAAGTTTGATTTTGCACAGTATTATGCTTTCAATCTGACAATATTTGCCCTAACCCTGATCTATTGTTCGTTTGCTCCTCTGGTGGTTCCTGTTGGTGCAGTTTACTTTGGGTACCGGTATTTAGTTGACAAGTACAACTTCCTGTTTGTATACAGAGTGCGAGGTTTCCCTGCTGGTAATGATGGGAGGTTGATGGATACTGTATTATCTATCATGAGGTTTTGTGTTGACTTGTTCCTCCTTTCAATGCTACTTTTCTTTTCTGTACGAGGAGACTCAACTAAGCTTCAAGCCATATTCACACTTGGATTGTTAGTGGTGTATAAACTCTTGCCCTCTGATAAGGATTCTTTTCAGCCAGCGTTATTACAAGGCATACAGACTATTGACAACATTGTCGAAGGGCCAACTGATTATGAGGTTTTCTCACAACCTACATTTGATTGGGATACGTATAATTCATGA
Protein Sequence
- >Solyc02g083430.1.1
MIQSSFSADSPSMAANSTFSPPPAAGDGDFNYDVAWYGNIQYLLNISAIGALTCLLIFIFGKLRSDHRRMPGPTAIVSKLLAAWHATGVEIARHCGADAAQYLLIEGGSSALLLFLALLSLAVMLPLNIYAGKAPMADQFSKTTINHIEKGSPLLWIHFIFVVIVVVLVHYGISEIQERLKITRLRDGYGNPSNSGTNVSAIFSIMVQGVPKTLGFDKTPLVEYFQHKYPGKVYRVVVPMDLCALDDLATELVKVREDISKLVSRIELRGYLNEGEEDEYNNDSVNGRGLLERLCFLWRKAKDTWYHVVDQLGFSDEERLRKLQELRADLEMEMASYKEGRARGAGVAFVVFKDVFTANKAVQDLRNEKRRRYGRFFSVIELQLQRNQWKVERAPLATDIYWNHLGSTKFSLKLRRVLVNTCLLLMLLFCSSPLAVISAIQSAGRIINAEAMDHAQMWLNWVQGSSWLATIIFQFLPNVLIFVSMYIVVPSVLSYLSKFEQHLTVSGEQRAELLKMVCFFLVNLILLRALVESTLEGALLSMGRCYLDGEDCKKIEQYMTASFLTRTCLSSLAFLITSSFLGISFDLLAPIPWIKKKLQKFRKNDMLQLVPERSEEYPLENQDIDSLERPLIHERSSTVIADNNGFLHDASPNEIDFPGQDLSEYPPVSRTSPVPKPKFDFAQYYAFNLTIFALTLIYCSFAPLVVPVGAVYFGYRYLVDKYNFLFVYRVRGFPAGNDGRLMDTVLSIMRFCVDLFLLSMLLFFSVRGDSTKLQAIFTLGLLVVYKLLPSDKDSFQPALLQGIQTIDNIVEGPTDYEVFSQPTFDWDTYNS*