Gene Details:

Functional Descriptions:

  • By construction of SlWRKY6 mutants and SlWRKY6-OE lines, we found that SlWRKY6 positively regulates leaf senescence and fruit ripening by activating the transcription of ripening-related genes STAYGREEN 1 (SlSGR1) and Senescence-Associated Gene 12 (SlSAG12).
  • Dual-luciferase transient expression assays and electrophoretic mobility shift assays indicated that SlWRKY6 persulfidation attenuated its transcriptional regulation of target genes SlSGR1 and SlSAG12, whereas SlWRKY6 phosphorylation by SlMAPK4 activated the transcription of target genes to promote fruit ripening.
  • SlWRKY6 persulfidation attenuated the expression of SlSGR1 and SlSAG12 thereby delaying tomato fruit ripening, while SlWRKY6 phosphorylation increased the expression of target genes.
  • Our findings reveal a model in which SlWRKY6 persulfidation due to higher endogenous H2S levels in un-ripened fruit inhibits its ability to activate SlSGR1 and SlSAG12 expression, while SlWRKY6 phosphorylation by SlMAPK4 activates its transcriptional activity, thereby promoting tomato fruit ripening.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Solyc02g080890.3.1
    ATAGCAGCGTCCATTGCATCTGCTAAAAACTTCTTAGACTCTTCAATTTATCATATTTCTATCTCTACCTTTTGATCTTCCAACTTTTATTTTTTTTATGGACAAAGGATGGGGTCTTACCCTTGAAACTTCTTCTTCTAATTCTGATAGAGCTGGTTTCTTCAAGAACAAGCCTGTTTTTGGTTTTAATTTAAGTCCTAAATTTAACGCTGCTGAAATGTTTTCCTCCTCCGATGAGAAACGTAGCATCGTTAATGAAGTCGACTTTTTCGCCGATAAAAAGCCTATTGTAAAGAAGGAAAATTCTCAGACCGATAATTCTATCAGAACTGATGATCAGTGTGTTGTAAATACTGGTTTGCAACTTGTGATTGCAAACGCGGGAAGTGATCAGTCAACGGTAGATGATGGGGTTTCATCGGAAGTACTTGAGGAGAAACGAGCTAAAATTCAGCAGTTGGCACAATTGCAAGTTGAGCTTGAACGGATGAATTCCGAAAATCAACGTTTAAAAGGGATGCTTACTCAAGTTAGCAACAGTTATTCTGCACTTCAGATGCATCTTGTTACACTCATGCAACAACAACTTAATTCAAGAACTGAAAATACACATCCCCATGAGGTTGTTGGCGCAAATTCTGATGAAGAAAAGAAAAAAGCGAATAATGGGACCATAGTTCCAAGACAATTCATGGAGCTAGGCCCTAGTGGTTCCAAAGTTGACCCAATGGACGAACCATCTCATTCTCGCTCTTCATCGGAAGAAAGAACACTCTCTGGATCACCTCGGAACAACATGGAATTAGTGTCAAGGGATAAGGCAATTAACCGCGAAGAAAGCCCAGAATCTGAGAGTTGGGCTCCAAACAAGGCCCCTAAATTGATGACTTCATCATCAAAGCCTGTTGAACAGTCAACAGAAGCAACCATGAGGAAAGCTCGTGTCTCCGTTCGTGCCCGATCAGAAGCTCCCATGATTAGTGATGGTTGCCAGTGGAGAAAATATGGTCAGAAAATGGCAAAAGGCAATCCATGTCCACGTGCTTATTATCGTTGCACTATGGCTGTTGGTTGTCCAGTCCGAAAACAGGTGCAAAGGTGTGCGGAGGACAGGTCGATCTTAATAACAACATACGAAGGTACACACAACCACCCATTACCACCAGCAGCCATGGCTATGGCATCTACAACATCTGCAGCAGCAAACATGTTGCTCTCTGGTTCCATGCCAAGTGCTGATGGGCTAATGAATACAAATTTCCTAGCAAGAGCAATGCTTCCATGTTCATCAAACATGGCAACTATTTCAGCTTCAGCCCCATTTCCTACTGTCACATTAGACCTCACAGCCCAAAATCCAAACGCAGCATTGCCTAATTATCATCAAAGAATCAACCAAGCAAATCCTCAATTCCAATTCCCTTTACCAGCTGGGCTAAATCACCCGAATTTCGCCGCTTCAATGTCAGCACCACAAATGCCTCACGTTTTGGGGCAGCCTTTGTATAACCAATCGAAATTTTCGGGTTTACAAATTTCTCAGGAAAACATTCATCATCACCACCCATCAATTTCTCATGACACACTCTCCGCCGCCACGGCTGCTATCACCGCCGATCCTAACTTCACCGCCGCCCTTGCGGCTGCCATCTCCTCCATCATCGGTGGTTCTCATCCGAACAACGGCAACAGTCCCATGTCCGGTCCCTCAAGCAATAATAATAACACTAGTAGTTTCCCAGGGAACTAATTAAATAAAATATTATTTTGTTTTGTAAAATTTGCTATTGTAGTCTTTCTTCATTCATTTTTTTAAAAAATTTGTTAGGACATTAGGGGATGAAATTGGAATGAATTTACATGTTCATATCTTTTTTCTTTGTATTATTACCGTGGAGGAAAGAGTATAGGAGAAAAAAAACGTTATTTTCATTCTTTTATTCTGATTTTGGTTATACGAACAAAAATGAATGCAATGTACTATGTGATTGCTTAATTA
CDS Sequence
  • >Solyc02g080890.3.1
    ATGGACAAAGGATGGGGTCTTACCCTTGAAACTTCTTCTTCTAATTCTGATAGAGCTGGTTTCTTCAAGAACAAGCCTGTTTTTGGTTTTAATTTAAGTCCTAAATTTAACGCTGCTGAAATGTTTTCCTCCTCCGATGAGAAACGTAGCATCGTTAATGAAGTCGACTTTTTCGCCGATAAAAAGCCTATTGTAAAGAAGGAAAATTCTCAGACCGATAATTCTATCAGAACTGATGATCAGTGTGTTGTAAATACTGGTTTGCAACTTGTGATTGCAAACGCGGGAAGTGATCAGTCAACGGTAGATGATGGGGTTTCATCGGAAGTACTTGAGGAGAAACGAGCTAAAATTCAGCAGTTGGCACAATTGCAAGTTGAGCTTGAACGGATGAATTCCGAAAATCAACGTTTAAAAGGGATGCTTACTCAAGTTAGCAACAGTTATTCTGCACTTCAGATGCATCTTGTTACACTCATGCAACAACAACTTAATTCAAGAACTGAAAATACACATCCCCATGAGGTTGTTGGCGCAAATTCTGATGAAGAAAAGAAAAAAGCGAATAATGGGACCATAGTTCCAAGACAATTCATGGAGCTAGGCCCTAGTGGTTCCAAAGTTGACCCAATGGACGAACCATCTCATTCTCGCTCTTCATCGGAAGAAAGAACACTCTCTGGATCACCTCGGAACAACATGGAATTAGTGTCAAGGGATAAGGCAATTAACCGCGAAGAAAGCCCAGAATCTGAGAGTTGGGCTCCAAACAAGGCCCCTAAATTGATGACTTCATCATCAAAGCCTGTTGAACAGTCAACAGAAGCAACCATGAGGAAAGCTCGTGTCTCCGTTCGTGCCCGATCAGAAGCTCCCATGATTAGTGATGGTTGCCAGTGGAGAAAATATGGTCAGAAAATGGCAAAAGGCAATCCATGTCCACGTGCTTATTATCGTTGCACTATGGCTGTTGGTTGTCCAGTCCGAAAACAGGTGCAAAGGTGTGCGGAGGACAGGTCGATCTTAATAACAACATACGAAGGTACACACAACCACCCATTACCACCAGCAGCCATGGCTATGGCATCTACAACATCTGCAGCAGCAAACATGTTGCTCTCTGGTTCCATGCCAAGTGCTGATGGGCTAATGAATACAAATTTCCTAGCAAGAGCAATGCTTCCATGTTCATCAAACATGGCAACTATTTCAGCTTCAGCCCCATTTCCTACTGTCACATTAGACCTCACAGCCCAAAATCCAAACGCAGCATTGCCTAATTATCATCAAAGAATCAACCAAGCAAATCCTCAATTCCAATTCCCTTTACCAGCTGGGCTAAATCACCCGAATTTCGCCGCTTCAATGTCAGCACCACAAATGCCTCACGTTTTGGGGCAGCCTTTGTATAACCAATCGAAATTTTCGGGTTTACAAATTTCTCAGGAAAACATTCATCATCACCACCCATCAATTTCTCATGACACACTCTCCGCCGCCACGGCTGCTATCACCGCCGATCCTAACTTCACCGCCGCCCTTGCGGCTGCCATCTCCTCCATCATCGGTGGTTCTCATCCGAACAACGGCAACAGTCCCATGTCCGGTCCCTCAAGCAATAATAATAACACTAGTAGTTTCCCAGGGAACTAA
Protein Sequence
  • >Solyc02g080890.3.1
    MDKGWGLTLETSSSNSDRAGFFKNKPVFGFNLSPKFNAAEMFSSSDEKRSIVNEVDFFADKKPIVKKENSQTDNSIRTDDQCVVNTGLQLVIANAGSDQSTVDDGVSSEVLEEKRAKIQQLAQLQVELERMNSENQRLKGMLTQVSNSYSALQMHLVTLMQQQLNSRTENTHPHEVVGANSDEEKKKANNGTIVPRQFMELGPSGSKVDPMDEPSHSRSSSEERTLSGSPRNNMELVSRDKAINREESPESESWAPNKAPKLMTSSSKPVEQSTEATMRKARVSVRARSEAPMISDGCQWRKYGQKMAKGNPCPRAYYRCTMAVGCPVRKQVQRCAEDRSILITTYEGTHNHPLPPAAMAMASTTSAAANMLLSGSMPSADGLMNTNFLARAMLPCSSNMATISASAPFPTVTLDLTAQNPNAALPNYHQRINQANPQFQFPLPAGLNHPNFAASMSAPQMPHVLGQPLYNQSKFSGLQISQENIHHHHPSISHDTLSAATAAITADPNFTAALAAAISSIIGGSHPNNGNSPMSGPSSNNNNTSSFPGN*