Gene Details:

• Gene ID: Solyc02g062460
• Gene Symbol: Sl23DOX
• Gene Name: C-23 hydroxylase
• Genome: Tomato Genome version SL4.0
• Species: Solanum lycopersicum

Functional Descriptions:

• Some 2-oxoglutarate-dependent dioxygenases were selected as candidates for the metabolic enzyme, and Solyc02g062460, designated Sl23DOX, was found to encode α-tomatine 23-hydroxylase.
• Biochemical analysis of the recombinant Sl23DOX protein demonstrated that it catalyzes the 23-hydroxylation of α-tomatine and the product spontaneously isomerizes to neorickiioside B, which is an intermediate in α-tomatine metabolism that appears during ripening.
• The ripe fruits of Sl23DOX-silenced transgenic tomato plants contained lower levels of esculeoside A but substantially accumulated α-tomatine.
• The metabolic detoxification of α-tomatine during tomato fruit ripening is an important trait that aided in its domestication, and two distinct 2-oxoglutarate-dependent dioxygenases (DOXs), a C-23 hydroxylase of α-tomatine (Sl23DOX) and a C-27 hydroxylase of lycoperoside C (Sl27DOX), are key to this process.
• Sl23DOX functions as α-tomatine 23-hydroxylase during the metabolic processing of toxic α-tomatine in tomato fruit ripening and is a key enzyme in the domestication of cultivated tomatoes.

Function-related keywords:

• domestication ,  detoxification ,  toxic ,  protein

Literature:

• Identification of α-Tomatine 23-Hydroxylase Involved in the Detoxification of a Bitter Glycoalkaloid.   DOI:  10.1093/pcp/pcz224 ;  PMID:  31816045
• Tandem Gene Duplication of Dioxygenases Drives the Structural Diversity of Steroidal Glycoalkaloids in the Tomato Clade.   DOI:  10.1093/pcp/pcac064 ;  PMID:  35560060

Related News:

Gene Resources:

• NCBI ID: 101246594
• UniProt accessions: A0A6F8PJG0

Orthologs:

Sequences: