Information report for Sl23DOX
Gene Details
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Functional Descriptions
- Some 2-oxoglutarate-dependent dioxygenases were selected as candidates for the metabolic enzyme, and Solyc02g062460, designated Sl23DOX, was found to encode α-tomatine 23-hydroxylase.
- Biochemical analysis of the recombinant Sl23DOX protein demonstrated that it catalyzes the 23-hydroxylation of α-tomatine and the product spontaneously isomerizes to neorickiioside B, which is an intermediate in α-tomatine metabolism that appears during ripening.
- The ripe fruits of Sl23DOX-silenced transgenic tomato plants contained lower levels of esculeoside A but substantially accumulated α-tomatine.
- The metabolic detoxification of α-tomatine during tomato fruit ripening is an important trait that aided in its domestication, and two distinct 2-oxoglutarate-dependent dioxygenases (DOXs), a C-23 hydroxylase of α-tomatine (Sl23DOX) and a C-27 hydroxylase of lycoperoside C (Sl27DOX), are key to this process.
- Sl23DOX functions as α-tomatine 23-hydroxylase during the metabolic processing of toxic α-tomatine in tomato fruit ripening and is a key enzyme in the domestication of cultivated tomatoes.
Functional Keywords
Literature and News
- Identification of α-Tomatine 23-Hydroxylase Involved in the Detoxification of a Bitter Glycoalkaloid. DOI: 10.1093/pcp/pcz224 ; PMID: 31816045
- Tandem Gene Duplication of Dioxygenases Drives the Structural Diversity of Steroidal Glycoalkaloids in the Tomato Clade. DOI: 10.1093/pcp/pcac064 ; PMID: 35560060
Gene Resources
- NCBI ID: 101246594
- UniProt accessions: A0A6F8PJG0
- EMBL: LC508578
- AlphaFoldDB: A0A6F8PJG0
- GO: GO:0002238 , GO:0009805 , GO:0016706 , GO:0031418 , GO:0046872
- InterPro: IPR005123 , IPR026992 , IPR027443 , IPR044861 , IPR050231
- PANTHER: PTHR47990
- SUPFAM: SSF51197
- PROSITE: PS51471
- Gene3D: 2.60.120.330
- Pfam: PF03171 , PF14226
- SMR: A0A6F8PJG0
Sequences
cDNA Sequence
- >Solyc02g062460.4.1
TGACTATAATTGATACTCAATCTGTTGAAATATAATGAACCAATTCTTATCAAAACACAGTGGAGTACAAGTAATCACGTTGGTTCCTATGAAATGGTTCATCTATTTCCCATTATATATAGGCTACTTATTTCCTCACCTATAAAGTAAAAAACTTTCTAGTGTTTTCTTCTTTCTTTTGTTTTTTTCTCTTTGCTCATATTCTAAAAATATTTCATCAATGGCATCTATCAAATCAGTTAAAGTTCCTACTATAGATTTTTCCAATTATCAAGAGCTAAAACCAAACACTCCACTATGGGAATCCACAAAAATTCAAGTTTTTGAAGCTTTACAAGAATATGGTTGTTTTGAAGCAATATATGATAAAGTTTCAAAGGAAATTAGAGAGGAAACATTTGATATGTCAAAAGAAATATTTGAATTTCCTTTAGAGACTAAAGTGAAAAATATCTCAGAAAAACCAATGCATGGCTATATGGGGATGATTCCACAATTGCCATTGTATGAGAGTTTGTGTATTCCTGATTTGCTTAATCCTCAAAGTCTTGAAAAATTTTCTAATATCTTTTGGCCTCAGGGTAATCAACATTTCTGCAATTTGATAAAATCTTATTCTAATCCACTTGTGGAATTGGATGGGATGTTGAAAAGGATGATTTCGGAGAATTTGGGATTGAAAAATCACATTGATGAATTATTGAATGCCAATTACTTCCTATTTAGATTTACACATTATAAGGGATCATCAATTGCTAGTGGAGATGAAAATAATAAAGCTGCTGGATTGGGTGGCCACACGGATGGTAACTTCTTGACTTTTATATCGCAAAATCAAGTTAATGGATTGCAAATCAACAAAAATGGAGAATGGATTGATGTGATTATTTCACCAAATTCTTACGTTGTTTTGGCCGGTGATTCCTTCAAAGCTTGGACAAATGGTCGATTGCATTCACCTCTCCACAGAGTAACAATGTCCGGACAAAATGATAGACTCTCCATTCAATTGTTTTCATTATCAAAGCCAGGTCACTTCATCCAGGCACCAAAAGAACTAGTAGATGAAGAACACCCATTACTCTTCAAGCCATTTGAAATTCTTGAATTATTCAAGTATGGTACCACAGAAGCTGGCTATACAGCTCCTCCAAGTGATCTTTTCAAGATTTATTGTGGTGTTTGATATGCTAATTGTTGAATTTCCGCTTCAACAAGCAACTTTTCTAATGAGTTTCATCTTGTTTTTTTAAGTAGTATGCATTTTATGTTTGAATTGTTGCAGTTGGCAATTCATGTTTAATTTGTTTTTGTTTTTTTGAGAAAATATTTCCAATGGGTTTCGTTGGAAATTCGTCTTGTTTTTTTTTTTCAAGTAGTGTACATCTTATTTTTGGATTGTTGATGTTGAGCGCTAATGTTTAATTTGTTTGTGTTTTGAAGAGGATGATTATACTCTTTAAGAGGATTCACCGTAATCTTTTAGTATTATTTG
CDS Sequence
- >Solyc02g062460.4.1
ATGGCATCTATCAAATCAGTTAAAGTTCCTACTATAGATTTTTCCAATTATCAAGAGCTAAAACCAAACACTCCACTATGGGAATCCACAAAAATTCAAGTTTTTGAAGCTTTACAAGAATATGGTTGTTTTGAAGCAATATATGATAAAGTTTCAAAGGAAATTAGAGAGGAAACATTTGATATGTCAAAAGAAATATTTGAATTTCCTTTAGAGACTAAAGTGAAAAATATCTCAGAAAAACCAATGCATGGCTATATGGGGATGATTCCACAATTGCCATTGTATGAGAGTTTGTGTATTCCTGATTTGCTTAATCCTCAAAGTCTTGAAAAATTTTCTAATATCTTTTGGCCTCAGGGTAATCAACATTTCTGCAATTTGATAAAATCTTATTCTAATCCACTTGTGGAATTGGATGGGATGTTGAAAAGGATGATTTCGGAGAATTTGGGATTGAAAAATCACATTGATGAATTATTGAATGCCAATTACTTCCTATTTAGATTTACACATTATAAGGGATCATCAATTGCTAGTGGAGATGAAAATAATAAAGCTGCTGGATTGGGTGGCCACACGGATGGTAACTTCTTGACTTTTATATCGCAAAATCAAGTTAATGGATTGCAAATCAACAAAAATGGAGAATGGATTGATGTGATTATTTCACCAAATTCTTACGTTGTTTTGGCCGGTGATTCCTTCAAAGCTTGGACAAATGGTCGATTGCATTCACCTCTCCACAGAGTAACAATGTCCGGACAAAATGATAGACTCTCCATTCAATTGTTTTCATTATCAAAGCCAGGTCACTTCATCCAGGCACCAAAAGAACTAGTAGATGAAGAACACCCATTACTCTTCAAGCCATTTGAAATTCTTGAATTATTCAAGTATGGTACCACAGAAGCTGGCTATACAGCTCCTCCAAGTGATCTTTTCAAGATTTATTGTGGTGTTTGA
Protein Sequence
- >Solyc02g062460.4.1
MASIKSVKVPTIDFSNYQELKPNTPLWESTKIQVFEALQEYGCFEAIYDKVSKEIREETFDMSKEIFEFPLETKVKNISEKPMHGYMGMIPQLPLYESLCIPDLLNPQSLEKFSNIFWPQGNQHFCNLIKSYSNPLVELDGMLKRMISENLGLKNHIDELLNANYFLFRFTHYKGSSIASGDENNKAAGLGGHTDGNFLTFISQNQVNGLQINKNGEWIDVIISPNSYVVLAGDSFKAWTNGRLHSPLHRVTMSGQNDRLSIQLFSLSKPGHFIQAPKELVDEEHPLLFKPFEILELFKYGTTEAGYTAPPSDLFKIYCGV*