Gene Details:
- Gene ID: Solyc01g095080
- Gene Symbol: LeACS2
- Gene Name: 1-aminocyclopropane-1-carboxylate synthase 2
- Genome: Tomato Genome version SL4.0
- Species: Solanum lycopersicum
Functional Descriptions:
- Turnover of LeACS2, a wound-inducible 1-aminocyclopropane-1-carboxylic acid synthase in tomato, is regulated by phosphorylation/dephosphorylation.
- Demonstrate that phosphorylation/dephosphorylation of LeACS2 regulates its turnover upstream of the ubiquitin-26S-proteasome degradation pathway.
- The amount of LeACS2 affected by the protein kinase/phosphatase inhibitors significantly influenced cellular ACS activity, ACC content, and ethylene production levels in tomato fruit tissue, suggesting that post-translational regulation by phosphorylation plays an important role in the control of ethylene production.
- LeACS2 was immediately phosphorylated after translation by CDPK and mitogen-activated protein kinase at different sites in response to wound signaling and almost all functional LeACS2 molecules are phosphorylated in the cell. Phosphorylation at both sites was required for LeACS2 stability.
Function-related keywords:
- kinase , ethylene , protein-kinase , ubiquitin , phosphatase , ethylene-response , protein-phosphatase , ethylene-production , protein-translation , ethylene-content , protein
Literature:
- Turnover of LeACS2, a wound-inducible 1-aminocyclopropane-1-carboxylic acid synthase in tomato, is regulated by phosphorylation/dephosphorylation. DOI: 10.1111/j.1365-313X.2010.04316.x ; PMID: 20659278
Related News:
Gene Resources:
Orthologs:
Sequences:
cDNA Sequence
- >Solyc01g095080.3.1
TTCCTTAATTCTCTTACACCATAACACAACTACAACAAACACATAATACTTTTAATACAATTAGTTATTTATTAGAAGTATTTAAAGTAAAGCACTTGTGAGTTGTGTACATTTTATTAATCTTCATCTTCTTAATTCTCTTCAGTTTTTAATTTCTTCACTTCTAAACTCATTTAGTAAAAAAAAAATGGGATTTGAGATTGCAAAGACCAACTCAATCTTATCAAAATTGGCTACTAATGAAGAGCATGGCGAAAACTCGCCATATTTTGATGGGTGGAAAGCATACGATAGTGATCCTTTCCACCCTCTAAAAAACCCCAACGGAGTTATCCAAATGGGTCTTGCTGAAAATCAGCTTTGTTTAGACTTGATAGAAGATTGGATTAAGAGAAACCCAAAAGGTTCAATTTGTTCTGAAGGAATCAAATCATTCAAGGCCATTGCCAACTTTCAAGATTATCATGGCTTGCCTGAATTCAGAAAAGCGATTGCGAAATTTATGGAGAAAACAAGAGGAGGAAGAGTTAGATTTGATCCAGAAAGAGTTGTTATGGCTGGTGGTGCCACTGGAGCTAATGAGACAATTATATTTTGTTTGGCTGATCCTGGCGATGCATTTTTAGTACCTTCACCATACTACCCAGCATTTAACAGAGATTTAAGATGGAGAACTGGAGTACAACTTATTCCAATTCACTGTGAGAGCTCCAATAATTTCAAAATTACTTCAAAAGCAGTAAAAGAAGCATATGAAAATGCACAAAAATCAAACATCAAAGTAAAAGGTTTGATTTTGACCAATCCATCAAATCCATTGGGCACCACTTTGGACAAAGACACACTGAAAAGTGTCTTGAGTTTCACCAACCAACACAACATCCACCTTGTTTGTGACGAAATCTACGCAGCCACTGTCTTTGACACGCCTCAATTCGTCAGTATAGCTGAAATCCTCGATGAACAGGAAATGACTTACTGCAACAAAGATTTAGTTCACATCGTCTACAGTCTTTCAAAAGACATGGGGTTACCAGGATTTAGAGTCGGAATCATATATTCTTTTAACGACGATGTCGTTAATTGTGCTAGAAAAATGTCGAGTTTCGGTTTAGTATCTACACAAACGCAATATTTTTTAGCGGCAATGCTATCGGACGAAAAATTCGTCGATAATTTTCTAAGAGAAAGCGCGATGAGGTTAGGTAAAAGGCACAAACATTTTACTAATGGACTTGAAGTAGTGGGAATTAAATGCTTGAAAAATAATGCGGGGCTTTTTTGTTGGATGGATTTGCGTCCACTTTTAAGGGAATCGACTTTCGATAGCGAAATGTCGTTATGGAGAGTTATTATAAACGATGTTAAGCTTAACGTCTCGCCTGGATCTTCGTTTGAATGTCAAGAGCCAGGGTGGTTCCGAGTTTGTTTTGCAAATATGGATGATGGAACGGTTGATATTGCGCTCGCGAGGATTCGGAGGTTCGTAGGTGTTGAGAAAAGTGGAGATAAATCGAGTTCGATGGAAAAGAAGCAACAATGGAAGAAGAATAATTTGAGACTTAGTTTTTCGAAAAGAATGTATGATGAAAGTGTTTTGTCACCACTTTCGTCACCTATTCCTCCCTCACCATTAGTTCGTTAAGACTTAATTAAAAGGGAAGAATTTAATTTATGTTTTTTTATATTTTGAAAAAAATTTGTAAGAATAAGATTATAATAGGAAAAGAAAATAAGTATGTAGGATGAGGAGTATTTTCAGAAATAGTTGTTAGCGTATGTATTGACAACTGGTCTATGTACTTAGACATCATAATTTGTCTTAGCTAATTAATGAATGCAAAAGTGAAGTTATGTTATGACTCTTAGAATCTTTTGATTTATTGGACTTTCTCGATTATATTGTTATT
CDS Sequence
- >Solyc01g095080.3.1
ATGGGATTTGAGATTGCAAAGACCAACTCAATCTTATCAAAATTGGCTACTAATGAAGAGCATGGCGAAAACTCGCCATATTTTGATGGGTGGAAAGCATACGATAGTGATCCTTTCCACCCTCTAAAAAACCCCAACGGAGTTATCCAAATGGGTCTTGCTGAAAATCAGCTTTGTTTAGACTTGATAGAAGATTGGATTAAGAGAAACCCAAAAGGTTCAATTTGTTCTGAAGGAATCAAATCATTCAAGGCCATTGCCAACTTTCAAGATTATCATGGCTTGCCTGAATTCAGAAAAGCGATTGCGAAATTTATGGAGAAAACAAGAGGAGGAAGAGTTAGATTTGATCCAGAAAGAGTTGTTATGGCTGGTGGTGCCACTGGAGCTAATGAGACAATTATATTTTGTTTGGCTGATCCTGGCGATGCATTTTTAGTACCTTCACCATACTACCCAGCATTTAACAGAGATTTAAGATGGAGAACTGGAGTACAACTTATTCCAATTCACTGTGAGAGCTCCAATAATTTCAAAATTACTTCAAAAGCAGTAAAAGAAGCATATGAAAATGCACAAAAATCAAACATCAAAGTAAAAGGTTTGATTTTGACCAATCCATCAAATCCATTGGGCACCACTTTGGACAAAGACACACTGAAAAGTGTCTTGAGTTTCACCAACCAACACAACATCCACCTTGTTTGTGACGAAATCTACGCAGCCACTGTCTTTGACACGCCTCAATTCGTCAGTATAGCTGAAATCCTCGATGAACAGGAAATGACTTACTGCAACAAAGATTTAGTTCACATCGTCTACAGTCTTTCAAAAGACATGGGGTTACCAGGATTTAGAGTCGGAATCATATATTCTTTTAACGACGATGTCGTTAATTGTGCTAGAAAAATGTCGAGTTTCGGTTTAGTATCTACACAAACGCAATATTTTTTAGCGGCAATGCTATCGGACGAAAAATTCGTCGATAATTTTCTAAGAGAAAGCGCGATGAGGTTAGGTAAAAGGCACAAACATTTTACTAATGGACTTGAAGTAGTGGGAATTAAATGCTTGAAAAATAATGCGGGGCTTTTTTGTTGGATGGATTTGCGTCCACTTTTAAGGGAATCGACTTTCGATAGCGAAATGTCGTTATGGAGAGTTATTATAAACGATGTTAAGCTTAACGTCTCGCCTGGATCTTCGTTTGAATGTCAAGAGCCAGGGTGGTTCCGAGTTTGTTTTGCAAATATGGATGATGGAACGGTTGATATTGCGCTCGCGAGGATTCGGAGGTTCGTAGGTGTTGAGAAAAGTGGAGATAAATCGAGTTCGATGGAAAAGAAGCAACAATGGAAGAAGAATAATTTGAGACTTAGTTTTTCGAAAAGAATGTATGATGAAAGTGTTTTGTCACCACTTTCGTCACCTATTCCTCCCTCACCATTAGTTCGTTAA
Protein Sequence
- >Solyc01g095080.3.1
MGFEIAKTNSILSKLATNEEHGENSPYFDGWKAYDSDPFHPLKNPNGVIQMGLAENQLCLDLIEDWIKRNPKGSICSEGIKSFKAIANFQDYHGLPEFRKAIAKFMEKTRGGRVRFDPERVVMAGGATGANETIIFCLADPGDAFLVPSPYYPAFNRDLRWRTGVQLIPIHCESSNNFKITSKAVKEAYENAQKSNIKVKGLILTNPSNPLGTTLDKDTLKSVLSFTNQHNIHLVCDEIYAATVFDTPQFVSIAEILDEQEMTYCNKDLVHIVYSLSKDMGLPGFRVGIIYSFNDDVVNCARKMSSFGLVSTQTQYFLAAMLSDEKFVDNFLRESAMRLGKRHKHFTNGLEVVGIKCLKNNAGLFCWMDLRPLLRESTFDSEMSLWRVIINDVKLNVSPGSSFECQEPGWFRVCFANMDDGTVDIALARIRRFVGVEKSGDKSSSMEKKQQWKKNNLRLSFSKRMYDESVLSPLSSPIPPSPLVR*