Gene Details:
- Gene ID: Solyc09g014720
- Gene Symbol: SlWak1
- Gene Name: wall-associated kinase 1
- Genome: Tomato Genome version SL4.0
- Species: Solanum lycopersicum
Functional Descriptions:
- SlWak1 coimmunoprecipitated with both Fls2 and Fls3, independently of flg22/flgII-28 or of BRASSINOSTEROID INSENSITIVE1-ASSOCIATED RECEPTOR KINASE1.
- In tomato (Solanum lycopersicum), wall-associated kinase 1 (SlWak1), has been implicated in pattern recognition receptor (PRR)-triggered immunity (PTI) because its transcript abundance increases significantly after treatment with the flagellin-derived, microbe-associated molecular patterns flg22 and flgII-28, which activate the PRRs Fls2 and Fls3, respectively. We generated two SlWak1 tomato mutants (Δwak1) using CRISPR/Cas9 gene editing technology and investigated the role of SlWak1 in tomato–Pst interactions.
- These observations suggest that SlWak1 acts in a complex with Fls2/Fls3 and is important at later stages of PTI in the apoplast.
- Tomato Wall-Associated Kinase SlWak1 Depends on Fls2/Fls3 to Promote Apoplastic Immune Responses to Pseudomonas syringae
Function-related keywords:
Literature:
- Tomato Wall-Associated Kinase SlWak1 Depends on Fls2/Fls3 to Promote Apoplastic Immune Responses to Pseudomonas syringae. DOI: 10.1104/pp.20.00144 ; PMID: 32371523
Related News:
Gene Resources:
- NCBI ID: 101263242
- UniProt accessions: A0A3Q7IVC3
Sequences:
cDNA Sequence
- >Solyc09g014720.3.1
ATGCAGCATTATCAAGTTGCTCTGTTTTCTTTCCAACTCCCCTGTTTTATGCTAATCTTAACACTAGCCACCGCCCAAATTATCCCTTCTAACACCACATCTCCGCCCACAAACTCCACTTCTCCGCCAACGAATGCCACCGCCCCCGCCCCCTCCCCCACCAACACAATCACCAAAGCTGCTAACATCACAAAACCAGGATGTCCAAAACAGTGTGGAAACGTTACAGTTCCATATCCATTTGGAATTGGGTCTGGTTGTGCCTTAGACCCCATGTTTGAGATCGACTGCAACGTTACAACACCATTCATAGGGAATATTCAAATTTACGATATCTCAGACGCTGAAATGCGTATATCAAATTTTATAAATACAAAGTGTTACTCACAAACAGGAGTTTTAATTCAAGATATTCCTTCTTGGATAACTTTAGGAACAAAAAGTCCTTACACTTTTTCTACCCTCAATAGATTCATCGTGGTTGGTTGTGATGACGGTGCTATAGTGAGTGGAAATAACTTTGCTAATGGTTGCCCCTCTCTCTGCACGAGCACCAATGATATAGTTAAAGGAAAATGTATGGGTTTTGGTTGCTGTCAAATTACAATACCAAAAGGCTTGAAATTTTTCAATACAACTATGGTAACCACAAGAAACCATAGCTTAATTTGGTCGTTTAATCCATGTGGACATTCGTTTCTTGGTGAAGCTAGTCGATTCGAGTTTCAGGGTATAGAGGATCTTAGTGATGTTAATTTTGCAAACAAGATTAGGAATAATGTGCCTATTGTGTTAGATTGGGCTATTGGAAATCTTTCTTGTGTTGAAGCACGAAAAAGTAACGATTATGCTTGCCTCAATAATAGCCAATGTGTTGATTCCGACACTAGCCTTGGTGGCTATCGGTGTAGCTGTAACTCGGGATATATAGGCAATCCATACATCGGCTCTGGCTGCCAAGATATCGATGAATGTGCGGATCCAAATACCAATTCATGTGAAAAGATTTGCACAAACATACCTGGGAGTTACAATTGTTCTTGTCCAGAAGGATACACCGGTGATGGCAGAAAGAATGGCCGTGGTTGTATTGCACCAAATTCGAACTCAGAGTTCCCATGGATCAAGTTTTCTGTTGGTATGGGAGTTGGTTTTATGTCACTAGTAATTGGGACAACTTGGCTCTATTTTTTCATCAAAAAAAGAAAACTCATTAAACTTCGCGAGAAATTCTTCCAACAAAATGGTGGTTTGCTTATGAAACAAAGAATGTCTTCTAACGAGGGTGGTGTTGAAGCAACCAAAATTTTCACTGCTGCAGAGCTGAAGAAGGCTACAAACAACTATGCCAGTGATAGAATTCTTGGTCGTGGTGGAAATGGAATTGTCTACAAAGGCATTCTATCTGATAATCGCATAGTTGCTATTAAGAAATCTAAGTTTATGGACGAGGAACAGGTTGAACAGTTCATTAACGAGGTACTTATTCTTACTCAAGTCAACCATAGAAATGTTGTGAGACTCTTCGGATGTTGTTTGGAAGCCGAAGTTCCTTTACTTGTCTATGAATACATTTCTCATGGAACTCTTTACGAGCATATCCACAATCGAAATGGAGCACCTTGGTTATCTTGGGAAAATCGGCTAAGAGTTGCTAGTGAGACAGCAAGTGCACTTGCTTACCTTCATTCATCCGCGCAAATGCCTATAATTCATAGAGATGTCAAGTCTGCCAATTTATTGTTGGATGATGTTTACATCGCGAAAGTGGCAGATTTTGGTGCTTCAAGGTTGATCCCTATTGATCAAACACATCTTGCTACAATGGTTCAAGGGACATTAGGATACCTAGATCCTGAATACTTTCGGACAAGTCAATTGACTGAGAAAAGTGATGTTTATAGTTTTGGAGTAGTTCTTGCCGAACTATTGACAGGGATGAAACCTATCTCCAAGGACAGAAATGAGGAGGAGAAGAATTTGGCAGAGTATTTTGTTTTGTCGATGAGAAGGAATCAATTGTTTCAAATTCTTGATCGTAGAGTTGTAAGAGAGGGAAGCCTCGAACAACTCCAAAAGGTGGCTGAGCTAGTGAAGAGCTGTCTTAGTTTGCACGGAGAAGATAGGCCTACCATGAAAGAAGTAGCAAGTGAACTTGAAAATTTGAGAAAGTTCACCAAAAATAACCCATGGGCTAATGGAAACGGACATGAAGAGAACGAAGATGAATTATCAGATCTTTATACAATTCCAATCGAGTCTAATACAGACATCGACAATTTCTCTGGACAATATAGTTCTAATTCCTATACAAATAGCAGCAACTTTTCTGGACAATATAGTTCAGGTAGTACTTCTAACACAAATAGCCCATTGATGAAAAACAGAAGGGCAATTTGA
CDS Sequence
- >Solyc09g014720.3.1
ATGCAGCATTATCAAGTTGCTCTGTTTTCTTTCCAACTCCCCTGTTTTATGCTAATCTTAACACTAGCCACCGCCCAAATTATCCCTTCTAACACCACATCTCCGCCCACAAACTCCACTTCTCCGCCAACGAATGCCACCGCCCCCGCCCCCTCCCCCACCAACACAATCACCAAAGCTGCTAACATCACAAAACCAGGATGTCCAAAACAGTGTGGAAACGTTACAGTTCCATATCCATTTGGAATTGGGTCTGGTTGTGCCTTAGACCCCATGTTTGAGATCGACTGCAACGTTACAACACCATTCATAGGGAATATTCAAATTTACGATATCTCAGACGCTGAAATGCGTATATCAAATTTTATAAATACAAAGTGTTACTCACAAACAGGAGTTTTAATTCAAGATATTCCTTCTTGGATAACTTTAGGAACAAAAAGTCCTTACACTTTTTCTACCCTCAATAGATTCATCGTGGTTGGTTGTGATGACGGTGCTATAGTGAGTGGAAATAACTTTGCTAATGGTTGCCCCTCTCTCTGCACGAGCACCAATGATATAGTTAAAGGAAAATGTATGGGTTTTGGTTGCTGTCAAATTACAATACCAAAAGGCTTGAAATTTTTCAATACAACTATGGTAACCACAAGAAACCATAGCTTAATTTGGTCGTTTAATCCATGTGGACATTCGTTTCTTGGTGAAGCTAGTCGATTCGAGTTTCAGGGTATAGAGGATCTTAGTGATGTTAATTTTGCAAACAAGATTAGGAATAATGTGCCTATTGTGTTAGATTGGGCTATTGGAAATCTTTCTTGTGTTGAAGCACGAAAAAGTAACGATTATGCTTGCCTCAATAATAGCCAATGTGTTGATTCCGACACTAGCCTTGGTGGCTATCGGTGTAGCTGTAACTCGGGATATATAGGCAATCCATACATCGGCTCTGGCTGCCAAGATATCGATGAATGTGCGGATCCAAATACCAATTCATGTGAAAAGATTTGCACAAACATACCTGGGAGTTACAATTGTTCTTGTCCAGAAGGATACACCGGTGATGGCAGAAAGAATGGCCGTGGTTGTATTGCACCAAATTCGAACTCAGAGTTCCCATGGATCAAGTTTTCTGTTGGTATGGGAGTTGGTTTTATGTCACTAGTAATTGGGACAACTTGGCTCTATTTTTTCATCAAAAAAAGAAAACTCATTAAACTTCGCGAGAAATTCTTCCAACAAAATGGTGGTTTGCTTATGAAACAAAGAATGTCTTCTAACGAGGGTGGTGTTGAAGCAACCAAAATTTTCACTGCTGCAGAGCTGAAGAAGGCTACAAACAACTATGCCAGTGATAGAATTCTTGGTCGTGGTGGAAATGGAATTGTCTACAAAGGCATTCTATCTGATAATCGCATAGTTGCTATTAAGAAATCTAAGTTTATGGACGAGGAACAGGTTGAACAGTTCATTAACGAGGTACTTATTCTTACTCAAGTCAACCATAGAAATGTTGTGAGACTCTTCGGATGTTGTTTGGAAGCCGAAGTTCCTTTACTTGTCTATGAATACATTTCTCATGGAACTCTTTACGAGCATATCCACAATCGAAATGGAGCACCTTGGTTATCTTGGGAAAATCGGCTAAGAGTTGCTAGTGAGACAGCAAGTGCACTTGCTTACCTTCATTCATCCGCGCAAATGCCTATAATTCATAGAGATGTCAAGTCTGCCAATTTATTGTTGGATGATGTTTACATCGCGAAAGTGGCAGATTTTGGTGCTTCAAGGTTGATCCCTATTGATCAAACACATCTTGCTACAATGGTTCAAGGGACATTAGGATACCTAGATCCTGAATACTTTCGGACAAGTCAATTGACTGAGAAAAGTGATGTTTATAGTTTTGGAGTAGTTCTTGCCGAACTATTGACAGGGATGAAACCTATCTCCAAGGACAGAAATGAGGAGGAGAAGAATTTGGCAGAGTATTTTGTTTTGTCGATGAGAAGGAATCAATTGTTTCAAATTCTTGATCGTAGAGTTGTAAGAGAGGGAAGCCTCGAACAACTCCAAAAGGTGGCTGAGCTAGTGAAGAGCTGTCTTAGTTTGCACGGAGAAGATAGGCCTACCATGAAAGAAGTAGCAAGTGAACTTGAAAATTTGAGAAAGTTCACCAAAAATAACCCATGGGCTAATGGAAACGGACATGAAGAGAACGAAGATGAATTATCAGATCTTTATACAATTCCAATCGAGTCTAATACAGACATCGACAATTTCTCTGGACAATATAGTTCTAATTCCTATACAAATAGCAGCAACTTTTCTGGACAATATAGTTCAGGTAGTACTTCTAACACAAATAGCCCATTGATGAAAAACAGAAGGGCAATTTGA
Protein Sequence
- >Solyc09g014720.3.1
MQHYQVALFSFQLPCFMLILTLATAQIIPSNTTSPPTNSTSPPTNATAPAPSPTNTITKAANITKPGCPKQCGNVTVPYPFGIGSGCALDPMFEIDCNVTTPFIGNIQIYDISDAEMRISNFINTKCYSQTGVLIQDIPSWITLGTKSPYTFSTLNRFIVVGCDDGAIVSGNNFANGCPSLCTSTNDIVKGKCMGFGCCQITIPKGLKFFNTTMVTTRNHSLIWSFNPCGHSFLGEASRFEFQGIEDLSDVNFANKIRNNVPIVLDWAIGNLSCVEARKSNDYACLNNSQCVDSDTSLGGYRCSCNSGYIGNPYIGSGCQDIDECADPNTNSCEKICTNIPGSYNCSCPEGYTGDGRKNGRGCIAPNSNSEFPWIKFSVGMGVGFMSLVIGTTWLYFFIKKRKLIKLREKFFQQNGGLLMKQRMSSNEGGVEATKIFTAAELKKATNNYASDRILGRGGNGIVYKGILSDNRIVAIKKSKFMDEEQVEQFINEVLILTQVNHRNVVRLFGCCLEAEVPLLVYEYISHGTLYEHIHNRNGAPWLSWENRLRVASETASALAYLHSSAQMPIIHRDVKSANLLLDDVYIAKVADFGASRLIPIDQTHLATMVQGTLGYLDPEYFRTSQLTEKSDVYSFGVVLAELLTGMKPISKDRNEEEKNLAEYFVLSMRRNQLFQILDRRVVREGSLEQLQKVAELVKSCLSLHGEDRPTMKEVASELENLRKFTKNNPWANGNGHEENEDELSDLYTIPIESNTDIDNFSGQYSSNSYTNSSNFSGQYSSGSTSNTNSPLMKNRRAI*