Gene Details:

Functional Descriptions:

  • SlWak1 coimmunoprecipitated with both Fls2 and Fls3, independently of flg22/flgII-28 or of BRASSINOSTEROID INSENSITIVE1-ASSOCIATED RECEPTOR KINASE1.
  • In tomato (Solanum lycopersicum), wall-associated kinase 1 (SlWak1), has been implicated in pattern recognition receptor (PRR)-triggered immunity (PTI) because its transcript abundance increases significantly after treatment with the flagellin-derived, microbe-associated molecular patterns flg22 and flgII-28, which activate the PRRs Fls2 and Fls3, respectively. We generated two SlWak1 tomato mutants (Δwak1) using CRISPR/Cas9 gene editing technology and investigated the role of SlWak1 in tomato–Pst interactions.
  • These observations suggest that SlWak1 acts in a complex with Fls2/Fls3 and is important at later stages of PTI in the apoplast.
  • Tomato Wall-Associated Kinase SlWak1 Depends on Fls2/Fls3 to Promote Apoplastic Immune Responses to Pseudomonas syringae

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Solyc09g014720.3.1
    ATGCAGCATTATCAAGTTGCTCTGTTTTCTTTCCAACTCCCCTGTTTTATGCTAATCTTAACACTAGCCACCGCCCAAATTATCCCTTCTAACACCACATCTCCGCCCACAAACTCCACTTCTCCGCCAACGAATGCCACCGCCCCCGCCCCCTCCCCCACCAACACAATCACCAAAGCTGCTAACATCACAAAACCAGGATGTCCAAAACAGTGTGGAAACGTTACAGTTCCATATCCATTTGGAATTGGGTCTGGTTGTGCCTTAGACCCCATGTTTGAGATCGACTGCAACGTTACAACACCATTCATAGGGAATATTCAAATTTACGATATCTCAGACGCTGAAATGCGTATATCAAATTTTATAAATACAAAGTGTTACTCACAAACAGGAGTTTTAATTCAAGATATTCCTTCTTGGATAACTTTAGGAACAAAAAGTCCTTACACTTTTTCTACCCTCAATAGATTCATCGTGGTTGGTTGTGATGACGGTGCTATAGTGAGTGGAAATAACTTTGCTAATGGTTGCCCCTCTCTCTGCACGAGCACCAATGATATAGTTAAAGGAAAATGTATGGGTTTTGGTTGCTGTCAAATTACAATACCAAAAGGCTTGAAATTTTTCAATACAACTATGGTAACCACAAGAAACCATAGCTTAATTTGGTCGTTTAATCCATGTGGACATTCGTTTCTTGGTGAAGCTAGTCGATTCGAGTTTCAGGGTATAGAGGATCTTAGTGATGTTAATTTTGCAAACAAGATTAGGAATAATGTGCCTATTGTGTTAGATTGGGCTATTGGAAATCTTTCTTGTGTTGAAGCACGAAAAAGTAACGATTATGCTTGCCTCAATAATAGCCAATGTGTTGATTCCGACACTAGCCTTGGTGGCTATCGGTGTAGCTGTAACTCGGGATATATAGGCAATCCATACATCGGCTCTGGCTGCCAAGATATCGATGAATGTGCGGATCCAAATACCAATTCATGTGAAAAGATTTGCACAAACATACCTGGGAGTTACAATTGTTCTTGTCCAGAAGGATACACCGGTGATGGCAGAAAGAATGGCCGTGGTTGTATTGCACCAAATTCGAACTCAGAGTTCCCATGGATCAAGTTTTCTGTTGGTATGGGAGTTGGTTTTATGTCACTAGTAATTGGGACAACTTGGCTCTATTTTTTCATCAAAAAAAGAAAACTCATTAAACTTCGCGAGAAATTCTTCCAACAAAATGGTGGTTTGCTTATGAAACAAAGAATGTCTTCTAACGAGGGTGGTGTTGAAGCAACCAAAATTTTCACTGCTGCAGAGCTGAAGAAGGCTACAAACAACTATGCCAGTGATAGAATTCTTGGTCGTGGTGGAAATGGAATTGTCTACAAAGGCATTCTATCTGATAATCGCATAGTTGCTATTAAGAAATCTAAGTTTATGGACGAGGAACAGGTTGAACAGTTCATTAACGAGGTACTTATTCTTACTCAAGTCAACCATAGAAATGTTGTGAGACTCTTCGGATGTTGTTTGGAAGCCGAAGTTCCTTTACTTGTCTATGAATACATTTCTCATGGAACTCTTTACGAGCATATCCACAATCGAAATGGAGCACCTTGGTTATCTTGGGAAAATCGGCTAAGAGTTGCTAGTGAGACAGCAAGTGCACTTGCTTACCTTCATTCATCCGCGCAAATGCCTATAATTCATAGAGATGTCAAGTCTGCCAATTTATTGTTGGATGATGTTTACATCGCGAAAGTGGCAGATTTTGGTGCTTCAAGGTTGATCCCTATTGATCAAACACATCTTGCTACAATGGTTCAAGGGACATTAGGATACCTAGATCCTGAATACTTTCGGACAAGTCAATTGACTGAGAAAAGTGATGTTTATAGTTTTGGAGTAGTTCTTGCCGAACTATTGACAGGGATGAAACCTATCTCCAAGGACAGAAATGAGGAGGAGAAGAATTTGGCAGAGTATTTTGTTTTGTCGATGAGAAGGAATCAATTGTTTCAAATTCTTGATCGTAGAGTTGTAAGAGAGGGAAGCCTCGAACAACTCCAAAAGGTGGCTGAGCTAGTGAAGAGCTGTCTTAGTTTGCACGGAGAAGATAGGCCTACCATGAAAGAAGTAGCAAGTGAACTTGAAAATTTGAGAAAGTTCACCAAAAATAACCCATGGGCTAATGGAAACGGACATGAAGAGAACGAAGATGAATTATCAGATCTTTATACAATTCCAATCGAGTCTAATACAGACATCGACAATTTCTCTGGACAATATAGTTCTAATTCCTATACAAATAGCAGCAACTTTTCTGGACAATATAGTTCAGGTAGTACTTCTAACACAAATAGCCCATTGATGAAAAACAGAAGGGCAATTTGA
CDS Sequence
  • >Solyc09g014720.3.1
    ATGCAGCATTATCAAGTTGCTCTGTTTTCTTTCCAACTCCCCTGTTTTATGCTAATCTTAACACTAGCCACCGCCCAAATTATCCCTTCTAACACCACATCTCCGCCCACAAACTCCACTTCTCCGCCAACGAATGCCACCGCCCCCGCCCCCTCCCCCACCAACACAATCACCAAAGCTGCTAACATCACAAAACCAGGATGTCCAAAACAGTGTGGAAACGTTACAGTTCCATATCCATTTGGAATTGGGTCTGGTTGTGCCTTAGACCCCATGTTTGAGATCGACTGCAACGTTACAACACCATTCATAGGGAATATTCAAATTTACGATATCTCAGACGCTGAAATGCGTATATCAAATTTTATAAATACAAAGTGTTACTCACAAACAGGAGTTTTAATTCAAGATATTCCTTCTTGGATAACTTTAGGAACAAAAAGTCCTTACACTTTTTCTACCCTCAATAGATTCATCGTGGTTGGTTGTGATGACGGTGCTATAGTGAGTGGAAATAACTTTGCTAATGGTTGCCCCTCTCTCTGCACGAGCACCAATGATATAGTTAAAGGAAAATGTATGGGTTTTGGTTGCTGTCAAATTACAATACCAAAAGGCTTGAAATTTTTCAATACAACTATGGTAACCACAAGAAACCATAGCTTAATTTGGTCGTTTAATCCATGTGGACATTCGTTTCTTGGTGAAGCTAGTCGATTCGAGTTTCAGGGTATAGAGGATCTTAGTGATGTTAATTTTGCAAACAAGATTAGGAATAATGTGCCTATTGTGTTAGATTGGGCTATTGGAAATCTTTCTTGTGTTGAAGCACGAAAAAGTAACGATTATGCTTGCCTCAATAATAGCCAATGTGTTGATTCCGACACTAGCCTTGGTGGCTATCGGTGTAGCTGTAACTCGGGATATATAGGCAATCCATACATCGGCTCTGGCTGCCAAGATATCGATGAATGTGCGGATCCAAATACCAATTCATGTGAAAAGATTTGCACAAACATACCTGGGAGTTACAATTGTTCTTGTCCAGAAGGATACACCGGTGATGGCAGAAAGAATGGCCGTGGTTGTATTGCACCAAATTCGAACTCAGAGTTCCCATGGATCAAGTTTTCTGTTGGTATGGGAGTTGGTTTTATGTCACTAGTAATTGGGACAACTTGGCTCTATTTTTTCATCAAAAAAAGAAAACTCATTAAACTTCGCGAGAAATTCTTCCAACAAAATGGTGGTTTGCTTATGAAACAAAGAATGTCTTCTAACGAGGGTGGTGTTGAAGCAACCAAAATTTTCACTGCTGCAGAGCTGAAGAAGGCTACAAACAACTATGCCAGTGATAGAATTCTTGGTCGTGGTGGAAATGGAATTGTCTACAAAGGCATTCTATCTGATAATCGCATAGTTGCTATTAAGAAATCTAAGTTTATGGACGAGGAACAGGTTGAACAGTTCATTAACGAGGTACTTATTCTTACTCAAGTCAACCATAGAAATGTTGTGAGACTCTTCGGATGTTGTTTGGAAGCCGAAGTTCCTTTACTTGTCTATGAATACATTTCTCATGGAACTCTTTACGAGCATATCCACAATCGAAATGGAGCACCTTGGTTATCTTGGGAAAATCGGCTAAGAGTTGCTAGTGAGACAGCAAGTGCACTTGCTTACCTTCATTCATCCGCGCAAATGCCTATAATTCATAGAGATGTCAAGTCTGCCAATTTATTGTTGGATGATGTTTACATCGCGAAAGTGGCAGATTTTGGTGCTTCAAGGTTGATCCCTATTGATCAAACACATCTTGCTACAATGGTTCAAGGGACATTAGGATACCTAGATCCTGAATACTTTCGGACAAGTCAATTGACTGAGAAAAGTGATGTTTATAGTTTTGGAGTAGTTCTTGCCGAACTATTGACAGGGATGAAACCTATCTCCAAGGACAGAAATGAGGAGGAGAAGAATTTGGCAGAGTATTTTGTTTTGTCGATGAGAAGGAATCAATTGTTTCAAATTCTTGATCGTAGAGTTGTAAGAGAGGGAAGCCTCGAACAACTCCAAAAGGTGGCTGAGCTAGTGAAGAGCTGTCTTAGTTTGCACGGAGAAGATAGGCCTACCATGAAAGAAGTAGCAAGTGAACTTGAAAATTTGAGAAAGTTCACCAAAAATAACCCATGGGCTAATGGAAACGGACATGAAGAGAACGAAGATGAATTATCAGATCTTTATACAATTCCAATCGAGTCTAATACAGACATCGACAATTTCTCTGGACAATATAGTTCTAATTCCTATACAAATAGCAGCAACTTTTCTGGACAATATAGTTCAGGTAGTACTTCTAACACAAATAGCCCATTGATGAAAAACAGAAGGGCAATTTGA
Protein Sequence
  • >Solyc09g014720.3.1
    MQHYQVALFSFQLPCFMLILTLATAQIIPSNTTSPPTNSTSPPTNATAPAPSPTNTITKAANITKPGCPKQCGNVTVPYPFGIGSGCALDPMFEIDCNVTTPFIGNIQIYDISDAEMRISNFINTKCYSQTGVLIQDIPSWITLGTKSPYTFSTLNRFIVVGCDDGAIVSGNNFANGCPSLCTSTNDIVKGKCMGFGCCQITIPKGLKFFNTTMVTTRNHSLIWSFNPCGHSFLGEASRFEFQGIEDLSDVNFANKIRNNVPIVLDWAIGNLSCVEARKSNDYACLNNSQCVDSDTSLGGYRCSCNSGYIGNPYIGSGCQDIDECADPNTNSCEKICTNIPGSYNCSCPEGYTGDGRKNGRGCIAPNSNSEFPWIKFSVGMGVGFMSLVIGTTWLYFFIKKRKLIKLREKFFQQNGGLLMKQRMSSNEGGVEATKIFTAAELKKATNNYASDRILGRGGNGIVYKGILSDNRIVAIKKSKFMDEEQVEQFINEVLILTQVNHRNVVRLFGCCLEAEVPLLVYEYISHGTLYEHIHNRNGAPWLSWENRLRVASETASALAYLHSSAQMPIIHRDVKSANLLLDDVYIAKVADFGASRLIPIDQTHLATMVQGTLGYLDPEYFRTSQLTEKSDVYSFGVVLAELLTGMKPISKDRNEEEKNLAEYFVLSMRRNQLFQILDRRVVREGSLEQLQKVAELVKSCLSLHGEDRPTMKEVASELENLRKFTKNNPWANGNGHEENEDELSDLYTIPIESNTDIDNFSGQYSSNSYTNSSNFSGQYSSGSTSNTNSPLMKNRRAI*