Gene Details:

Functional Descriptions:

  • Overexpressing SlTLFP8 significantly decreased stomatal density, while SlTLFP8 knockout resulted in increased stomatal density, and SlTLFP8 affected stomatal density through endoreduplication and altered epidermal cell size. Despite changes in stomata density, altering the expression of SlTLFP8 did not result in distinct changes in photosynthesis, biomass and yield of tomato.
  • SlTLFP8 reduces water loss to improve water-use efficiency by modulating cell size and stomatal density via endoreduplication.
  • SlTLFP8 (Tubby-like F-box protein 8) was identified as an osmotic-induced gene in tomato.
  • Transgenic tomato with up-regulated expression of SlTLFP8 showed enhanced water-deficient resistance, whereas knockout mutants generated by CRISPR/Cas9 were more sensitive to water deficit.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Solyc01g104670.3.1
    TTGAAAGCAATTATATCTTTTATACTCATTTTCTTGCAACTAAGCTAGGACAACTGGCATGATGCAGCACCTTAAGAATCTAAAGAAGTTGCATATGTTAAAAGTCATGTGATTTACTTTATTTAGGTGCCCATGTGATGATGTCTCTTTATACTCTTTTCTTTTCCCTTTCCCTCTTTTTTTTCTTTTAATGCCTTTCTTGCTTTTTCGTCTTCGTTTCTCTTCAGAAATCTTTTCTTGTTACTGAAATACGGCTTGTTTTCTTGTTCTTTTGCAGCTGTAACTATTTGTCAAGCTTTCTTTAAGATACTGGGAGCTTGTATGGTATGAAGAGTAAATAGGCCAGTAACATTTAATAGTGGTGCTTGGCCTTTGACCTTTCAGTTTCAAGTTCAGATGTCGTTCCGCAGTATAGTTCGTGATGTAAGAGATAGTATTGGTAGCTTATCAAGGCGAAGCTTTGATTTAAGGCTGTCTGGTCATCCTAGGGACAAATCACATGGTTCATTTTATGACTTAAGTGACCAGCCTCCCGTTATCCAAGATAGTTGTTGGGCCAATCTTCCTCCAGAGCTACTTTTTGATGTAGTTAGAAGGTTAGAGGAGAGTGAGAGCACATGGCCCGGTCGTAAGCATGTCGTAGCATGTGCCGCAGTCTGTAGGTCATGGAGGAGTATGTGCAAAGACATCGTTAGAAATCCGGAATTTTGTGGAAAGCTAACTTTTCCTGTTTCCCTAAAGCAGCCTGGGCCTCGTGATGGAACTATTCAGTGCTTCATCAAGCGGGATAAATCTAATTTGACTTACCATCTTTTCTTGTGTCTTAGTCCTGCATTGTTGGTTGAGAATGGGAAGTTCCTTCTCTCTGCAAAAAGAACGAGACGTACTACTTGCACAGAGTATGTTATCTCAATGGATGCAGAAAACATCTCTAGATCAAGCAACACCTATATTGGAAAATTAAGATCAAATTTTCTAGGTACAAAATTCATTATATATGACACACAGCCTCCTCACACCGGTGCACATGTACCTCCCCCGGGGCGAACAAGCCGTAGATTCACTTCCAAGAAAGTCTCTCCTAAAGTCCCAACTGGAAGCTATATCATATCCCAGATCACATACGAGCTGAATGTGCTTGGAACACGGGGTCCACGGAGAATGCACTGCGTCATGCACTCAATTCCTGACTCAGCCCTTGAAGCCGGTGGCTCTGTACCTGGCCAACCAGAGCTTCTCTCAAGGCCCCTTGAGGATTCGTTCCGCAGCATCTCTTTCTCAAAGTCTCTTGATCATTCCACTGAGTTCGGTAGTGCACGATTTTCTGATATTGCTGGAGGATCAACTAATGAGGAGGATGATAACAAGGGGAAACCACTGGTCCTAAAGAACAAGGCGCCACGATGGCATGAACAACTGCAGTGCTGGTGTCTGAATTTTCGAGGACGAGTGACAGTTGCATCTGTCAAGAACTTCCAGTTGATTGCTGCCACTCAACAACCTGCCGCTGCACCAACAACATCTCAGCCAACAAGTCAATCAGATCATGACAAAATCATCTTGCAATTTGGGAAAGTAGGCAAAGATATGTTTACCATGGACTACCGATATCCCCTATCTGCATTTCAGGCATTTGCCATCTGCTTAAGCAGCTTTGACACAAAATTGGCTTGTGAATAGAAGAGCTAAAACAGTTTTACATTTAGGGTGCTATTAACTGTAATGCTTCTATTCTGGATTTGTTGTACTTCTTTCTTCTTTTCACAATTGAAAATGGAGATTGTCCCTAAAATTACGCCATGGCGTAAAACCCTGCTTCCTGCGAAAAGTTGTAAAAGAAATCTATTTCTGATCACTGCCATTTACAAGAATTTCCACCAGAAAATGAGAGGACGCTAGATATTGATGCGAATGTAATAAGAGATAAAACGATTAATTCGTGACTTGAAA
CDS Sequence
  • >Solyc01g104670.3.1
    ATGTCGTTCCGCAGTATAGTTCGTGATGTAAGAGATAGTATTGGTAGCTTATCAAGGCGAAGCTTTGATTTAAGGCTGTCTGGTCATCCTAGGGACAAATCACATGGTTCATTTTATGACTTAAGTGACCAGCCTCCCGTTATCCAAGATAGTTGTTGGGCCAATCTTCCTCCAGAGCTACTTTTTGATGTAGTTAGAAGGTTAGAGGAGAGTGAGAGCACATGGCCCGGTCGTAAGCATGTCGTAGCATGTGCCGCAGTCTGTAGGTCATGGAGGAGTATGTGCAAAGACATCGTTAGAAATCCGGAATTTTGTGGAAAGCTAACTTTTCCTGTTTCCCTAAAGCAGCCTGGGCCTCGTGATGGAACTATTCAGTGCTTCATCAAGCGGGATAAATCTAATTTGACTTACCATCTTTTCTTGTGTCTTAGTCCTGCATTGTTGGTTGAGAATGGGAAGTTCCTTCTCTCTGCAAAAAGAACGAGACGTACTACTTGCACAGAGTATGTTATCTCAATGGATGCAGAAAACATCTCTAGATCAAGCAACACCTATATTGGAAAATTAAGATCAAATTTTCTAGGTACAAAATTCATTATATATGACACACAGCCTCCTCACACCGGTGCACATGTACCTCCCCCGGGGCGAACAAGCCGTAGATTCACTTCCAAGAAAGTCTCTCCTAAAGTCCCAACTGGAAGCTATATCATATCCCAGATCACATACGAGCTGAATGTGCTTGGAACACGGGGTCCACGGAGAATGCACTGCGTCATGCACTCAATTCCTGACTCAGCCCTTGAAGCCGGTGGCTCTGTACCTGGCCAACCAGAGCTTCTCTCAAGGCCCCTTGAGGATTCGTTCCGCAGCATCTCTTTCTCAAAGTCTCTTGATCATTCCACTGAGTTCGGTAGTGCACGATTTTCTGATATTGCTGGAGGATCAACTAATGAGGAGGATGATAACAAGGGGAAACCACTGGTCCTAAAGAACAAGGCGCCACGATGGCATGAACAACTGCAGTGCTGGTGTCTGAATTTTCGAGGACGAGTGACAGTTGCATCTGTCAAGAACTTCCAGTTGATTGCTGCCACTCAACAACCTGCCGCTGCACCAACAACATCTCAGCCAACAAGTCAATCAGATCATGACAAAATCATCTTGCAATTTGGGAAAGTAGGCAAAGATATGTTTACCATGGACTACCGATATCCCCTATCTGCATTTCAGGCATTTGCCATCTGCTTAAGCAGCTTTGACACAAAATTGGCTTGTGAATAG
Protein Sequence
  • >Solyc01g104670.3.1
    MSFRSIVRDVRDSIGSLSRRSFDLRLSGHPRDKSHGSFYDLSDQPPVIQDSCWANLPPELLFDVVRRLEESESTWPGRKHVVACAAVCRSWRSMCKDIVRNPEFCGKLTFPVSLKQPGPRDGTIQCFIKRDKSNLTYHLFLCLSPALLVENGKFLLSAKRTRRTTCTEYVISMDAENISRSSNTYIGKLRSNFLGTKFIIYDTQPPHTGAHVPPPGRTSRRFTSKKVSPKVPTGSYIISQITYELNVLGTRGPRRMHCVMHSIPDSALEAGGSVPGQPELLSRPLEDSFRSISFSKSLDHSTEFGSARFSDIAGGSTNEEDDNKGKPLVLKNKAPRWHEQLQCWCLNFRGRVTVASVKNFQLIAATQQPAAAPTTSQPTSQSDHDKIILQFGKVGKDMFTMDYRYPLSAFQAFAICLSSFDTKLACE*