Gene Details:

Functional Descriptions:

  • In tomato (Solanum lycopersicum L.), drought and salt stress stimulate nitric oxide (NO) production, which boosts proline synthesis by activating Δ1-pyrroline-5-carboxylate synthetase (SlP5CS) and Δ1-pyrroline-5-carboxylate reductase (SlP5CR) genes and the P5CR enzyme.
  • The crucial factor is stress-triggered NO production, which regulates the S-nitrosylation of SlP5CR at Cys-5, thereby increasing its NAD(P)H affinity and enzymatic activity.
  • S-nitrosylation of SlP5CR enables tomato plants to better adapt to changing NAD(P)H levels, boosting both SlP5CR activity and proline synthesis during stress.
  • This study demonstrates that tomato engineered to mimic S-nitrosylated SlP5CR exhibits enhanced growth and yield under drought and salt stress conditions, highlighting a promising approach for stress-tolerant tomato cultivation.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Solyc02g068640.3.1
    GGCGATGTATTTAACAAAAAATTTGTTTAAAATAGTACCTAATATATAGACCTAGAAATACAAATTTGTGCCGAGTATTAAGGCCCTAAACAGTCGCTTAAATAAGAAGAAGAGTGATGCATTAAAACACATCAGCTACTCTTCGTCTTGCCGCCTTCACTGATTTCAGAAGAAAAGTTGTATCTTTACTGTTACATAATTTCCAAATGGCGAATGTTTGTCCAATTCCAACCGATTCATACAAGCTGGGCTTTATAGGAGCTGGTAAAATGGCTGAAAGTATAGCTAGAGGTGTGGTTAAGTCTGGGATATTGCCTGCTTCTCGTATTCGAACAGCCCATTCTGGATCAGCTCGTCGTACTGCTTTTGAGTCTATTGGGGTTACTGTTTTAGATAATAATAGTCAGGTGGTTGAAGATAGTGATGTGATTATATTCTCTGTGAAGCCTCAAGTGGTTAAAAATGTGGTGTCAGAGTTGAAGCCAATCCTTTCAGAGAAGAAGCTTTTGGTGTCAGTTGCTGCTGGTATTAAGTTAAAGGATTTGCAGGAATGGGCAGGTCAAGGCCGATTTATTAGGGTGATGCCAAACACTCCCTCTGCTGTTGGTGAAGCCGCCACTGTCATCACTTTGGGGGAAAAGGCAACAACCGAAGATGGAGAGTTAATTTCCCAGTTATTTGGAGCTATTGGTAAGGTGTGGAAAGCTGATGAGAAGCTGTTTGATGCAGTTACAGGTTTAAGTGGTAGTGGACCAGCCTATGTTTTTCTAGCAATAGAGGCATTGGCTGATGGAGGGGTGGCTGCTGGTTTACCGAGGGAGCTTGCACTTGGACTAGCCTCTCAAACCGTACTAGGAGCAGCATCAATGGTCTCCGGTATGAGCAAGCATCCTGGTCAACATAAAGATGATGTTGCATCACCTGGGGGTACAACCATTGCTGGAATTCACGAGTTGGAGAAGTCTGGATTTCGCGGTATCTTGATGAATGCAGTTGTTGCTGCAGCTAAACGAAGTAAAGAGCTTTCTCAGAATTAATCTACTTATCTCTATTAAGGTCATGGAGAACCAGCCGGAAGCTATTATATATGTCTAGCAGAGTTTTTTAAGTGCTAAATTATTATGTGAAAGGTGCTGAGCTTTTTGACCAAAATAAAGCACCGTATCTGTTAAACTTTTTGGACCAACATGCCCACATTTTATTAAAGAGGCTTGTGGCACAATCACAATCACTATAAATAAACATTATTTATAGTTAT
CDS Sequence
  • >Solyc02g068640.3.1
    ATGGCGAATGTTTGTCCAATTCCAACCGATTCATACAAGCTGGGCTTTATAGGAGCTGGTAAAATGGCTGAAAGTATAGCTAGAGGTGTGGTTAAGTCTGGGATATTGCCTGCTTCTCGTATTCGAACAGCCCATTCTGGATCAGCTCGTCGTACTGCTTTTGAGTCTATTGGGGTTACTGTTTTAGATAATAATAGTCAGGTGGTTGAAGATAGTGATGTGATTATATTCTCTGTGAAGCCTCAAGTGGTTAAAAATGTGGTGTCAGAGTTGAAGCCAATCCTTTCAGAGAAGAAGCTTTTGGTGTCAGTTGCTGCTGGTATTAAGTTAAAGGATTTGCAGGAATGGGCAGGTCAAGGCCGATTTATTAGGGTGATGCCAAACACTCCCTCTGCTGTTGGTGAAGCCGCCACTGTCATCACTTTGGGGGAAAAGGCAACAACCGAAGATGGAGAGTTAATTTCCCAGTTATTTGGAGCTATTGGTAAGGTGTGGAAAGCTGATGAGAAGCTGTTTGATGCAGTTACAGGTTTAAGTGGTAGTGGACCAGCCTATGTTTTTCTAGCAATAGAGGCATTGGCTGATGGAGGGGTGGCTGCTGGTTTACCGAGGGAGCTTGCACTTGGACTAGCCTCTCAAACCGTACTAGGAGCAGCATCAATGGTCTCCGGTATGAGCAAGCATCCTGGTCAACATAAAGATGATGTTGCATCACCTGGGGGTACAACCATTGCTGGAATTCACGAGTTGGAGAAGTCTGGATTTCGCGGTATCTTGATGAATGCAGTTGTTGCTGCAGCTAAACGAAGTAAAGAGCTTTCTCAGAATTAA
Protein Sequence
  • >Solyc02g068640.3.1
    MANVCPIPTDSYKLGFIGAGKMAESIARGVVKSGILPASRIRTAHSGSARRTAFESIGVTVLDNNSQVVEDSDVIIFSVKPQVVKNVVSELKPILSEKKLLVSVAAGIKLKDLQEWAGQGRFIRVMPNTPSAVGEAATVITLGEKATTEDGELISQLFGAIGKVWKADEKLFDAVTGLSGSGPAYVFLAIEALADGGVAAGLPRELALGLASQTVLGAASMVSGMSKHPGQHKDDVASPGGTTIAGIHELEKSGFRGILMNAVVAAAKRSKELSQN*