Gene Details:
- Gene ID: Solyc07g056570
- Gene Symbol: SlNCED1
- Gene Name: 9-cis-epoxycarotenoid dioxygenase
- Genome: Tomato Genome version SL4.0
- Species: Solanum lycopersicum
Functional Descriptions:
- SlNCED1 and SlCYP707A2: key genes involved in ABA metabolism during tomato fruit ripening.
- Quantitative real-time PCR showed that VIGS-treated tomato fruits had significant reductions in target gene transcripts.
- Silencing SlNCED1 or SlCYP707A2 by VIGS significantly altered the transcripts of a set of both ABA-responsive and ripening-related genes, including ABA-signalling genes (PYL1, PP2C1, and SnRK2.2), lycopene-synthesis genes (SlBcyc, SlPSY1 and SlPDS), and cell wall-degrading genes (SlPG1, SlEXP, and SlXET) during ripening.
- These data indicate that SlNCED1 and SlCYP707A2 are key genes in the regulation of ABA synthesis and catabolism, and are involved in fruit ripening as positive and negative regulators, respectively.
- Fruit-specific RNAi-mediated suppression of SlNCED1 increases both lycopene and beta-carotene contents in tomato fruit.
- To study the regulation of carotenoid biosynthesis by ABA, the SlNCED1 gene encoding 9-cis-epoxycarotenoid dioxygenase (NCED), a key enzyme in the ABA biosynthesis, was suppressed in tomato plants by transformation with an RNA interference (RNAi) construct driven by a fruit-specific E8 promoter.
Function-related keywords:
- aba , cell-wall , cell-wall-synthesis , aba-biosynthesis , aba-catabolism , carotene , carotenoid , carotenoid-biosynthesis
Literature:
- SlNCED1 and SlCYP707A2: key genes involved in ABA metabolism during tomato fruit ripening. DOI: 10.1093/jxb/eru288 ; PMID: 25039074
- Fruit-specific RNAi-mediated suppression of SlNCED1 increases both lycopene and β-carotene contents in tomato fruit. DOI: 10.1093/jxb/ers026 ; PMID: 22345638
Related News:
Gene Resources:
Orthologs:
Sequences:
cDNA Sequence
- >Solyc07g056570.1.1
ATGGCAACTACTACTTCACATGCCACAAATACATGGATTAAGACTAAGTTGTCAATGCCATCATCAAAGGAGTTTGGTTTTGCATCAAACTCTATTTCTCTACTCAAAAATCAACATAATAGGCAAAGTCTCAACATTAATTCCTCTCTTCAAGCTCCACCTATACTTCATTTTCCTAAACAATCTTCAAATTATCAAACACCAAAGAATAATACAATTTCACACCCAAAACAAGAAAACAACAACTCCTCTTCTTCTTCAACTTCCAAGTGGAATTTAGTGCAGAAAGCAGCAGCAATGGCTTTAGATGCTGTAGAAAGTGCTTTAACTAAACATGAACTTGAACACCCTTTGCCGAAAACAGCCGACCCACGAGTCCAGATTTCTGGGAATTTTGCTCCGGTACCGGAAAATCCAGTCTGTCAATCTCTTCCGGTCACCGGAAAAATACCCAAATGTGTTCAAGGCGTTTACGTTCGAAACGGAGCTAACCCTCTTTTTGAACCAACCGCCGGACACCATTTCTTCGACGGCGACGGTATGGTTCACGCCGTTCAATTCAAAAATGGGTCGGCTAGTTACGCTTGCCGTTTCACTGAAACAGAGAGGCTTGTTCAAGAAAAAGCTTTGGGTCGCCCTGTTTTCCCTAAAGCCATTGGTGAATTACATGGTCACTCTGGAATTGCAAGGCTTATGCTGTTTTACGCTCGTGGGCTCTTCGGACTTGTTGATCACAGTAAAGGAACTGGTGTTGCAAACGCCGGTTTAGTCTATTTCAATAACCGATTACTTGCTATGTCTGAAGATGATTTGCCTTACCATGTAAAGGTAACACCCACCGGCGATCTTAAAACAGAGGGTCGATTCGATTTCGACGGCCAGCTAAAATCCACCATGATAGCTCACCCAAAGCTCGACCCAGTTTCCGGTGAGCTATTTGCTCTTAGCTACGATGTGATTCAGAAGCCATACCTCAAGTACTTCAGATTTTCAAAAAATGGGGAAAAATCAAATGATGTTGAAATTCCAGTTGAAGACCCAACAATGATGCATGATTTCGCAATTACTGAGAACTTCGTCGTCATTCCTGATCAACAAGTCGTTTTCAAGATGTCTGAAATGATCCGTGGAGGTTCACCGGTGGTTTACGACAAGAACAAAGTTTCCCGATTTGGTATTCTGGATAAGTACGCGAAAGATGGGTCTGATTTGAAATGGGTTGAAGTACCTGATTGTTTCTGTTTCCACCTCTGGAATGCTTGGGAAGAAGCAGAAACAGATGAAATCGTTGTAATTGGTTCATGTATGACACCACCAGACTCCATTTTCAATGAATGTGATGAAGGGCTAAAGAGTGTTTTATCCGAAATCCGTCTCAATTTGAAAACAGGGAAATCAACAAGAAAATCCATAATCGAAAACCCGGATGAACAAGTGAATTTAGAAGCTGGAATGGTGAACCGAAACAAACTCGGAAGGAAAACAGAGTATGCTTATTTGGCTATCGCTGAACCATGGCCAAAAGTTTCTGGTTTTGCAAAAGTAAACCTGTTCACCGGTGAAGTTGAGAAATTCATTTATGGTGACAACAAATATGGTGGGGAACCTCTTTTTTTACCAAGAGACCCCAACAGCAAGGAAGAAGACGATGGTTATATTTTAGCTTTCGTTCACGATGAGAAAGAATGGAAATCAGAACTGCAAATTGTTAACGCAATGAGTTTGAAGTTGGAGGCAACTGTGAAGCTTCCATCAAGAGTTCCTTATGGATTTCATGGAACATTCATAAACGCCAATGATTTGGCAAATCAGGCATGA
CDS Sequence
- >Solyc07g056570.1.1
ATGGCAACTACTACTTCACATGCCACAAATACATGGATTAAGACTAAGTTGTCAATGCCATCATCAAAGGAGTTTGGTTTTGCATCAAACTCTATTTCTCTACTCAAAAATCAACATAATAGGCAAAGTCTCAACATTAATTCCTCTCTTCAAGCTCCACCTATACTTCATTTTCCTAAACAATCTTCAAATTATCAAACACCAAAGAATAATACAATTTCACACCCAAAACAAGAAAACAACAACTCCTCTTCTTCTTCAACTTCCAAGTGGAATTTAGTGCAGAAAGCAGCAGCAATGGCTTTAGATGCTGTAGAAAGTGCTTTAACTAAACATGAACTTGAACACCCTTTGCCGAAAACAGCCGACCCACGAGTCCAGATTTCTGGGAATTTTGCTCCGGTACCGGAAAATCCAGTCTGTCAATCTCTTCCGGTCACCGGAAAAATACCCAAATGTGTTCAAGGCGTTTACGTTCGAAACGGAGCTAACCCTCTTTTTGAACCAACCGCCGGACACCATTTCTTCGACGGCGACGGTATGGTTCACGCCGTTCAATTCAAAAATGGGTCGGCTAGTTACGCTTGCCGTTTCACTGAAACAGAGAGGCTTGTTCAAGAAAAAGCTTTGGGTCGCCCTGTTTTCCCTAAAGCCATTGGTGAATTACATGGTCACTCTGGAATTGCAAGGCTTATGCTGTTTTACGCTCGTGGGCTCTTCGGACTTGTTGATCACAGTAAAGGAACTGGTGTTGCAAACGCCGGTTTAGTCTATTTCAATAACCGATTACTTGCTATGTCTGAAGATGATTTGCCTTACCATGTAAAGGTAACACCCACCGGCGATCTTAAAACAGAGGGTCGATTCGATTTCGACGGCCAGCTAAAATCCACCATGATAGCTCACCCAAAGCTCGACCCAGTTTCCGGTGAGCTATTTGCTCTTAGCTACGATGTGATTCAGAAGCCATACCTCAAGTACTTCAGATTTTCAAAAAATGGGGAAAAATCAAATGATGTTGAAATTCCAGTTGAAGACCCAACAATGATGCATGATTTCGCAATTACTGAGAACTTCGTCGTCATTCCTGATCAACAAGTCGTTTTCAAGATGTCTGAAATGATCCGTGGAGGTTCACCGGTGGTTTACGACAAGAACAAAGTTTCCCGATTTGGTATTCTGGATAAGTACGCGAAAGATGGGTCTGATTTGAAATGGGTTGAAGTACCTGATTGTTTCTGTTTCCACCTCTGGAATGCTTGGGAAGAAGCAGAAACAGATGAAATCGTTGTAATTGGTTCATGTATGACACCACCAGACTCCATTTTCAATGAATGTGATGAAGGGCTAAAGAGTGTTTTATCCGAAATCCGTCTCAATTTGAAAACAGGGAAATCAACAAGAAAATCCATAATCGAAAACCCGGATGAACAAGTGAATTTAGAAGCTGGAATGGTGAACCGAAACAAACTCGGAAGGAAAACAGAGTATGCTTATTTGGCTATCGCTGAACCATGGCCAAAAGTTTCTGGTTTTGCAAAAGTAAACCTGTTCACCGGTGAAGTTGAGAAATTCATTTATGGTGACAACAAATATGGTGGGGAACCTCTTTTTTTACCAAGAGACCCCAACAGCAAGGAAGAAGACGATGGTTATATTTTAGCTTTCGTTCACGATGAGAAAGAATGGAAATCAGAACTGCAAATTGTTAACGCAATGAGTTTGAAGTTGGAGGCAACTGTGAAGCTTCCATCAAGAGTTCCTTATGGATTTCATGGAACATTCATAAACGCCAATGATTTGGCAAATCAGGCATGA
Protein Sequence
- >Solyc07g056570.1.1
MATTTSHATNTWIKTKLSMPSSKEFGFASNSISLLKNQHNRQSLNINSSLQAPPILHFPKQSSNYQTPKNNTISHPKQENNNSSSSSTSKWNLVQKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPVPENPVCQSLPVTGKIPKCVQGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACRFTETERLVQEKALGRPVFPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTGVANAGLVYFNNRLLAMSEDDLPYHVKVTPTGDLKTEGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNGEKSNDVEIPVEDPTMMHDFAITENFVVIPDQQVVFKMSEMIRGGSPVVYDKNKVSRFGILDKYAKDGSDLKWVEVPDCFCFHLWNAWEEAETDEIVVIGSCMTPPDSIFNECDEGLKSVLSEIRLNLKTGKSTRKSIIENPDEQVNLEAGMVNRNKLGRKTEYAYLAIAEPWPKVSGFAKVNLFTGEVEKFIYGDNKYGGEPLFLPRDPNSKEEDDGYILAFVHDEKEWKSELQIVNAMSLKLEATVKLPSRVPYGFHGTFINANDLANQA*