Gene Details:

Functional Descriptions:

  • Here, we isolated homologous genes of rice Si influx (SlLsi1) and efflux (SlLsi2) transporter genes in tomato (Solanum lycopersicum L.) and functionally characterized these genes.
  • Two Lsi2-like proteins did not show efflux transport activity for Si in Xenopus oocytes.
  • SlLsi2 has different AA residues from any of the rest Lsi2s that have been demonstrated to have Si efflux transport activity.
  • Our results suggest that the low Si accumulation in tomato is attributed to the lack of functional Si efflux transporter Lsi2 required for active Si uptake although SlLsi1 is functional.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Solyc03g025350.3.1
    AAGGACTCTTGTGAAATAAAGTTCACATATTATAGGTAAGGTCATTTTCTCATCGCCTTGCAACACAAAAATCTAAGGTTTGTTAGCTTTGTTTTTACATTTCAGGTATATTTATTACTAATGTGAACATACAAAAAACTCAAAGAAGGATTATGACTATGGCATTGACGGAGAGAGTGATTCTTGGTTCCATTGCTTTTGCAATATTTTGGATATTGGCAGTTTTTCCTGCTGTACCGTTTATGCCTATTGGAAGGACTGCAGGATCATTACTTGGTGCTAATTTAATCGTAATATTTCGAGTATTGACACCAGATGAAGCATATGCTGCTATTGATCTTCCAATTTTAGGACTTCTGTTTGGAACAATGGTTGTGAGTATTTATTTAGAAAGAGCAGATATGTTTAAGTACTTGGGAAAATTATTGGCTTGGAAAAGCAGGGGAGCTAAGGATTTACTCTGTAGAATCTGTTTGGTTTCTGCAATTTCAAGTGCTTTTTTCACTAATGATACTAGTTGTGTTGTGTTAACTGAGTTTGTGCTGAAAATTGCTAGGCAACAAAATCTCCCACCTCATCCGTTCCTTTTAGCTCTCGCGTCTAGTGCAAATATTGGATCTTCAGCAACTCCTATTGGGAATCCTCAGAACTTAGTTATAGCAATATTGAGCAAGATCACGTTCGGGAGGTTTTTATTTGGTATATTTCCTGCAATGTTTGTTGGAGTTGTTGTTAATGCTGTATTGTTGCTTTGCATGTATTGGAATGTGTTGTCTGTTCAGAAGGATGTTGAAAAAGCTGAAGTAGAATTAGTTTTGGATGAACAAGTAGTCTCTCATCGTTTTGCACCAGCAACGATGTCACATGTTGTTAACTCGTTACATTCTCAAGATCAGTTTAGTGGGAATGAAATCATCAAGGATCCAAACGGGGTGTTTGATTCTTCTAGGAATTTTAATGCCTCGAGAGAAGCAGCAAATGATCATGGATCACTTCAAATGAGGGACAAGAATGTGTCTTTGAAGACTGTCGATGAACGTGAAGATCAAAACTTCACATCTTGTGAGGAAAAGCACAACTTCTCTGAAATATGGAGAAGATTGTTGTGGAAAATATGTGTTTATCTTGTTACAATTGGAATGTTAATATCTTTATTGATGGGGCTGAATATGTCATGGACTGCTATCACAGCAGCACTTGCTCTTGTGGTTCTTGATTTCAAAGATGCTAAAGAATGCTTAGAAAAGGTATCGTATTCGCTGTTGATATTCTTCTGTGGTATGTTTATAACAGTAGATGGATTCAACAGAACAGGGATTCCAAGTGCTTTTTGGGATTTTATGGAACCATATGCCAAGATAGATCATGCTGGTGGTGTAGCAGTTCTTGCTGTTGTCATACTTGTCCTCTCAAATTTAGCTTCTAATGTGCCTACTGTTCTATTGCTGGGAGTTCGGGTAGGTGCATCAGCGGGTGCAATATCCGAAGCAAGTGAGAAGAAAGCGTGGCTCATCTTAGCCTGGGTTAGTACTGTGGCTGGCAACCTTTCACTATTAGGGTCAGCAGCAAACTTGATAGTATGTGAACAAGCTCGTCGCGCTCAACCTTCGGGGTACAATCTCTCATTCTGGACTCATCTCAAATTTGGAGTTCCCTCTACTATAATTGTTATAGCTATTGGTTTGATACTCATTGGAGATTGAAGTTAAATTTGTACTAATAGCTTCACAATGATAGTCAATAGGAGTTAAGTCTACCTTGTGTATTCTGTTCTCCCAACAACATGTTGTAAACAAGTGCAGCTAAAACTGTCACTTGGTATAATTTCCAATTTCATTATAAT
CDS Sequence
  • >Solyc03g025350.3.1
    ATGACTATGGCATTGACGGAGAGAGTGATTCTTGGTTCCATTGCTTTTGCAATATTTTGGATATTGGCAGTTTTTCCTGCTGTACCGTTTATGCCTATTGGAAGGACTGCAGGATCATTACTTGGTGCTAATTTAATCGTAATATTTCGAGTATTGACACCAGATGAAGCATATGCTGCTATTGATCTTCCAATTTTAGGACTTCTGTTTGGAACAATGGTTGTGAGTATTTATTTAGAAAGAGCAGATATGTTTAAGTACTTGGGAAAATTATTGGCTTGGAAAAGCAGGGGAGCTAAGGATTTACTCTGTAGAATCTGTTTGGTTTCTGCAATTTCAAGTGCTTTTTTCACTAATGATACTAGTTGTGTTGTGTTAACTGAGTTTGTGCTGAAAATTGCTAGGCAACAAAATCTCCCACCTCATCCGTTCCTTTTAGCTCTCGCGTCTAGTGCAAATATTGGATCTTCAGCAACTCCTATTGGGAATCCTCAGAACTTAGTTATAGCAATATTGAGCAAGATCACGTTCGGGAGGTTTTTATTTGGTATATTTCCTGCAATGTTTGTTGGAGTTGTTGTTAATGCTGTATTGTTGCTTTGCATGTATTGGAATGTGTTGTCTGTTCAGAAGGATGTTGAAAAAGCTGAAGTAGAATTAGTTTTGGATGAACAAGTAGTCTCTCATCGTTTTGCACCAGCAACGATGTCACATGTTGTTAACTCGTTACATTCTCAAGATCAGTTTAGTGGGAATGAAATCATCAAGGATCCAAACGGGGTGTTTGATTCTTCTAGGAATTTTAATGCCTCGAGAGAAGCAGCAAATGATCATGGATCACTTCAAATGAGGGACAAGAATGTGTCTTTGAAGACTGTCGATGAACGTGAAGATCAAAACTTCACATCTTGTGAGGAAAAGCACAACTTCTCTGAAATATGGAGAAGATTGTTGTGGAAAATATGTGTTTATCTTGTTACAATTGGAATGTTAATATCTTTATTGATGGGGCTGAATATGTCATGGACTGCTATCACAGCAGCACTTGCTCTTGTGGTTCTTGATTTCAAAGATGCTAAAGAATGCTTAGAAAAGGTATCGTATTCGCTGTTGATATTCTTCTGTGGTATGTTTATAACAGTAGATGGATTCAACAGAACAGGGATTCCAAGTGCTTTTTGGGATTTTATGGAACCATATGCCAAGATAGATCATGCTGGTGGTGTAGCAGTTCTTGCTGTTGTCATACTTGTCCTCTCAAATTTAGCTTCTAATGTGCCTACTGTTCTATTGCTGGGAGTTCGGGTAGGTGCATCAGCGGGTGCAATATCCGAAGCAAGTGAGAAGAAAGCGTGGCTCATCTTAGCCTGGGTTAGTACTGTGGCTGGCAACCTTTCACTATTAGGGTCAGCAGCAAACTTGATAGTATGTGAACAAGCTCGTCGCGCTCAACCTTCGGGGTACAATCTCTCATTCTGGACTCATCTCAAATTTGGAGTTCCCTCTACTATAATTGTTATAGCTATTGGTTTGATACTCATTGGAGATTGA
Protein Sequence
  • >Solyc03g025350.3.1
    MTMALTERVILGSIAFAIFWILAVFPAVPFMPIGRTAGSLLGANLIVIFRVLTPDEAYAAIDLPILGLLFGTMVVSIYLERADMFKYLGKLLAWKSRGAKDLLCRICLVSAISSAFFTNDTSCVVLTEFVLKIARQQNLPPHPFLLALASSANIGSSATPIGNPQNLVIAILSKITFGRFLFGIFPAMFVGVVVNAVLLLCMYWNVLSVQKDVEKAEVELVLDEQVVSHRFAPATMSHVVNSLHSQDQFSGNEIIKDPNGVFDSSRNFNASREAANDHGSLQMRDKNVSLKTVDEREDQNFTSCEEKHNFSEIWRRLLWKICVYLVTIGMLISLLMGLNMSWTAITAALALVVLDFKDAKECLEKVSYSLLIFFCGMFITVDGFNRTGIPSAFWDFMEPYAKIDHAGGVAVLAVVILVLSNLASNVPTVLLLGVRVGASAGAISEASEKKAWLILAWVSTVAGNLSLLGSAANLIVCEQARRAQPSGYNLSFWTHLKFGVPSTIIVIAIGLILIGD*