Gene Details:

Functional Descriptions:

  • Auxin-mediated regulation of arbuscular mycorrhizal symbiosis: A role of SlGH3.4 in tomato.
  • An AM-induced GH3 gene, SlGH3.4, encoding a putative IAA-amido synthetase, negatively regulates mycorrhization via maintaining cellular auxin homoeostasis.
  • Loss of SlGH3.4 function increased free IAA content and arbuscule incidence, while constitutively overexpressing SlGH3.4 in either tomato or rice resulted in decreased IAA content, total colonisation level and arbuscule abundance in mycorrhizal roots.
  • Our results highlight a positive correlation between the endogenous IAA content and mycorrhization level, particularly arbuscule incidence, and suggest that the SlGH3.4-mediated auxin homoeostasis and regulation of expansin genes is involved in finely tuning the AM development.
  • The characterization of six auxin-induced tomato GH3 genes uncovers a member, SlGH3.4, strongly responsive to arbuscular mycorrhizal symbiosis.
  • A comparison of the SlGH3.4 expression in wild-type plants and M161, a mutant with a defect in AM symbiosis, confirmed that SlGH3.4 expression is highly correlated to mycorrhizal colonization.

Literature:

Gene Resources:

Sequences:

cDNA Sequence
  • >Solyc02g092820.4.1
    CACACAATTGAAATAAACAATAATCTTATTCTGCTTTAAATTCTCACTTCCACTTTTTTTAAATAATGGCTATTGTTTCTGGTCTTTCATCTCCTCTTGGTCCTCCGGCATGTGAAAAAGATGCCAAGGTCCTTCAGTTTATCGATGACATGACTAAAAATATTGATCCTGAACAAGTGAAAGTATTGGCTGAAATATTAACCCGAAATGCCAATACTGAGTACCTCCGGAGGTTCAACATTAATGGTGCTATTGACCGTGAAACTTTCAAGTCTAAGGTCCCCGTTGTTACATACGAGGATCTTAAACCTGATATTGATCGTATTGCTAATGGTGATCGTTCTCCCATTTTCTCATCACACCCTATCTCCGAATTCCTTACCAGTTCGGGGACGTCTGCTGGAGAGAGAAAACTGATGCCCACAATTGCGGATGAGCTAGAACGCAGACAAAAACTGTACAACCTTCTACAGCCCGTGATGAATCTGTATGTGCCTGGTTTAGATAAGGGGAAGGGATTGTACTTTCTATTTATCAAGGCAGAAACGAAGACGCCTAGTGGCCTTGTTGCACGTCCAGTCCTAACAAGTTACTACAAAAGTGAACAATTCAAGACAAGGCCATACGATCCATACAATGTGTATACAAGCCCCAACGAGACCATTCTCTGTGTCGATTCATTCCAAAGCATGTATTCCCAAATGCTTTGTGGCCTTCTCATGAGAGAAGAAGTCCTCCGAGTTGGTGCTGTTTTTGCCTCTGGCTTACTCCGTGCCATCCGTTTTCTTCAACTCAATTGGAAGCAATTGTCTCAGGATATCGCGTCTGGAACTCTAAACCCTAGAGTTTCAGACCCGTCTATCCGAAATTGCATTTCCCAAATACTCAAACCAAATCCCCAACTTGCTGAGTTTGTTGCTAAGGAATGTGAAGGCGAAAATTGGGAAGGAATCATTACTAGAATTTGGCCAAACACAAAGCACCTTGATGTGATAGTCACAGGTGCAATGGCTCAATATATACCTACTTTGGATTATTATAGTGGGGGTCTACCAAAGGTGTGTACAATGTACGCATCATCCGAATGTTATTTTGGACTCAATTTAAATCCATTGTGTAAGCCTTGTGAAGTTTGTTACACCATTATGCCAAATATGGGTTACTTTGAATTCATCCCTCATGACCCGGTCAACCCGGTTCAAATCTCTCGTGACTCACCCCCACATCTAGTAGACCTTGCTGACGTGGAAATTGGAAAAGAATACGAGCTCGTGATCACAACTTACGCCGGACTATGCCGTTACCGAGTTGGTGACATACTCCAGGTCACCGGGTTCCACAACTCGGCCCCTCAGTTCAAATTCGTCAGGCGAAAAAACGTGCTGTTGAGTATTGATTCTGATAAAACCGATGAGTCCGAATTACACACCGCGATTGAAAATGCGACCGCACTTTTGCGCCCGTATGATACTACTTTAGTTGAGTACACCAGTTTTGCCGATACTAAGACAATTCCGGGGCATTACGTTATTTACTGGGAGTTACTTGTTAAGGACCCGGCTAACCCACCCAGTGATGACGTGTTGAACCAGTGCTGCCTTGCGATGGAACAATCATTGAACTCGGTGTACAGGCAGTGTCGCGTCGCAGACAATTCAATCGGGCCACTGGAGATAAGAGTGGTGAAAAACGGGACATTTGAAGAACTTATGGATTATGCAATTTCGAGAGGTGCATCAATTAATCAGTATAAGGCACCAAGGTGTGTTAATTTTACTCCTATTGTTGAACTTTTGGACTCCAGGGTGATTTCTGTGAATTTCAGTCCAAAAGCTCCTCACTGGACTCCAGAACGACGACGTTGAACACTAGGCATGCTATTTTATCAAGTCAAGTAGTGTTGTTAGTGTGTGAAAAAAAAAATGTTTAGACAGTGGACCAAAGAAAGAAAAAAAAAGAATCAGTACTATAATTCTTCGTCTCTGTCTGATCACTTTAATTTGCTTTCTTTTTGTGAATCTGATATCTGTTGTTGGATGTTGAATATTGTTCAAAGTTGATTATGGTCTTTGCTGATGTAAA
CDS Sequence
  • >Solyc02g092820.4.1
    ATGGCTATTGTTTCTGGTCTTTCATCTCCTCTTGGTCCTCCGGCATGTGAAAAAGATGCCAAGGTCCTTCAGTTTATCGATGACATGACTAAAAATATTGATCCTGAACAAGTGAAAGTATTGGCTGAAATATTAACCCGAAATGCCAATACTGAGTACCTCCGGAGGTTCAACATTAATGGTGCTATTGACCGTGAAACTTTCAAGTCTAAGGTCCCCGTTGTTACATACGAGGATCTTAAACCTGATATTGATCGTATTGCTAATGGTGATCGTTCTCCCATTTTCTCATCACACCCTATCTCCGAATTCCTTACCAGTTCGGGGACGTCTGCTGGAGAGAGAAAACTGATGCCCACAATTGCGGATGAGCTAGAACGCAGACAAAAACTGTACAACCTTCTACAGCCCGTGATGAATCTGTATGTGCCTGGTTTAGATAAGGGGAAGGGATTGTACTTTCTATTTATCAAGGCAGAAACGAAGACGCCTAGTGGCCTTGTTGCACGTCCAGTCCTAACAAGTTACTACAAAAGTGAACAATTCAAGACAAGGCCATACGATCCATACAATGTGTATACAAGCCCCAACGAGACCATTCTCTGTGTCGATTCATTCCAAAGCATGTATTCCCAAATGCTTTGTGGCCTTCTCATGAGAGAAGAAGTCCTCCGAGTTGGTGCTGTTTTTGCCTCTGGCTTACTCCGTGCCATCCGTTTTCTTCAACTCAATTGGAAGCAATTGTCTCAGGATATCGCGTCTGGAACTCTAAACCCTAGAGTTTCAGACCCGTCTATCCGAAATTGCATTTCCCAAATACTCAAACCAAATCCCCAACTTGCTGAGTTTGTTGCTAAGGAATGTGAAGGCGAAAATTGGGAAGGAATCATTACTAGAATTTGGCCAAACACAAAGCACCTTGATGTGATAGTCACAGGTGCAATGGCTCAATATATACCTACTTTGGATTATTATAGTGGGGGTCTACCAAAGGTGTGTACAATGTACGCATCATCCGAATGTTATTTTGGACTCAATTTAAATCCATTGTGTAAGCCTTGTGAAGTTTGTTACACCATTATGCCAAATATGGGTTACTTTGAATTCATCCCTCATGACCCGGTCAACCCGGTTCAAATCTCTCGTGACTCACCCCCACATCTAGTAGACCTTGCTGACGTGGAAATTGGAAAAGAATACGAGCTCGTGATCACAACTTACGCCGGACTATGCCGTTACCGAGTTGGTGACATACTCCAGGTCACCGGGTTCCACAACTCGGCCCCTCAGTTCAAATTCGTCAGGCGAAAAAACGTGCTGTTGAGTATTGATTCTGATAAAACCGATGAGTCCGAATTACACACCGCGATTGAAAATGCGACCGCACTTTTGCGCCCGTATGATACTACTTTAGTTGAGTACACCAGTTTTGCCGATACTAAGACAATTCCGGGGCATTACGTTATTTACTGGGAGTTACTTGTTAAGGACCCGGCTAACCCACCCAGTGATGACGTGTTGAACCAGTGCTGCCTTGCGATGGAACAATCATTGAACTCGGTGTACAGGCAGTGTCGCGTCGCAGACAATTCAATCGGGCCACTGGAGATAAGAGTGGTGAAAAACGGGACATTTGAAGAACTTATGGATTATGCAATTTCGAGAGGTGCATCAATTAATCAGTATAAGGCACCAAGGTGTGTTAATTTTACTCCTATTGTTGAACTTTTGGACTCCAGGGTGATTTCTGTGAATTTCAGTCCAAAAGCTCCTCACTGGACTCCAGAACGACGACGTTGA
Protein Sequence
  • >Solyc02g092820.4.1
    MAIVSGLSSPLGPPACEKDAKVLQFIDDMTKNIDPEQVKVLAEILTRNANTEYLRRFNINGAIDRETFKSKVPVVTYEDLKPDIDRIANGDRSPIFSSHPISEFLTSSGTSAGERKLMPTIADELERRQKLYNLLQPVMNLYVPGLDKGKGLYFLFIKAETKTPSGLVARPVLTSYYKSEQFKTRPYDPYNVYTSPNETILCVDSFQSMYSQMLCGLLMREEVLRVGAVFASGLLRAIRFLQLNWKQLSQDIASGTLNPRVSDPSIRNCISQILKPNPQLAEFVAKECEGENWEGIITRIWPNTKHLDVIVTGAMAQYIPTLDYYSGGLPKVCTMYASSECYFGLNLNPLCKPCEVCYTIMPNMGYFEFIPHDPVNPVQISRDSPPHLVDLADVEIGKEYELVITTYAGLCRYRVGDILQVTGFHNSAPQFKFVRRKNVLLSIDSDKTDESELHTAIENATALLRPYDTTLVEYTSFADTKTIPGHYVIYWELLVKDPANPPSDDVLNQCCLAMEQSLNSVYRQCRVADNSIGPLEIRVVKNGTFEELMDYAISRGASINQYKAPRCVNFTPIVELLDSRVISVNFSPKAPHWTPERRR*