Information report for SlGAD3
Gene Details
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Functional Descriptions
- Tomato Glutamate Decarboxylase Genes SlGAD2 and SlGAD3 Play Key Roles in Regulating γ-Aminobutyric Acid Levels in Tomato.
- We previously identified three tomato genes (SlGAD1, SlGAD2 and SlGAD3) encoding glutamate decarboxylase (GAD), likely the key enzyme for GABA biosynthesis in tomato fruits.
- A significant decrease in GABA levels, i.e. 50–81% compared with wild-type (WT) levels, was observed in mature green (MG) fruits of the SlGAD2-suppressed lines, while a more drastic reduction (up to <10% of WT levels) was observed in the SlGAD3- and triple SlGAD-suppressed lines.
- These findings suggest that both SlGAD2 and SlGAD3 expression are crucial for GABA biosynthesis in tomato fruits.
- We also determined that strong down-regulation of the SlGADs had little effect on overall plant growth, fruit development or primary fruit metabolism under normal growth conditions.
Functional Keywords
Literature and News
Gene Resources
- NCBI ID: 100147723
- UniProt accessions: B1Q3F2
- EMBL: AB359915
- EnsemblPlants: Solyc01g005000.3.1
- Gramene: Solyc01g005000.3.1
- KEGG: sly:100147723
- GO: GO:0004351 , GO:0005516 , GO:0005829 , GO:0006538 , GO:0030170
- OMA: RMPEESI
- InterPro: IPR002129 , IPR010107 , IPR015421 , IPR015424
- PANTHER: PTHR43321 , PTHR43321:SF27
- SUPFAM: SSF53383
- Gene3D: 3.40.640.10 , 3.90.1150.160 , 4.10.280.50
- OrthoDB: B1Q3F2
- Pfam: PF00282
- SMR: B1Q3F2
- STRING: 4081.B1Q3F2
- HOGENOM: CLU_019582_2_2_1
Sequences
cDNA Sequence
- >Solyc01g005000.3.1
ATTGGACAACTATAAATAGCCATAGCCCTTTCTCCCAACACACAAACAAACAAACTTTAATTAATTCTCCAAAAACTTTTTAATTATTTACAACAACATAATGGTTCTCTCAAAAACTCCTTCTGATGATTCTGTACACTCCACATTTGCTTCTCGCTATGTTCGAACTTCACTACCAAGGTTTGAGATGCTAGAGAAGTCTATACCAAAAGAGGCAGCATACCAAATGATTAATGATGAGTTAATGCTTGATGGGAATCCAAGGTTAAATTTGGCATCATTTGTAACCACATGGATGGAACCAGAATGTGATAAGCTTATGATGGCTTCAATTAACAAGAATTATGTTGACATGGATGAATACCCTGTCACCACTGAGCTTCAGAATCGATGTGTAAACATGATAGCGCGTTTATTCAATGCGCCTTTGAAAGAGGAAGAAATAGGAATTGGTGTGGGGACAGTGGGGTCATCAGAGGCCATAATGTTAGCAGGGCTGGCCTTCAAGAGGAACTGGCAAAACAAACGCAAAGCTGAGGGAAAGCCTTATGATAAGCCCAACATTGTCACTGGTGCTAATGTTCAGGTGTGTTGGGAGAAATTTGCAAACTACTTTGAAGTGGAATTGAAACAAGTCAAGTTAAGTGAAGGGTACTATGTGATGGACCCAATCAAAGCTGTGGAAATGGTAGATGACAACACTATTTGTGTTGCTGCTATTTTGGGTTCAACACTTAATGGAGAATTTGAAGATGTCAAACTCTTGAATGATCTTTTGATTGAAAAGAATAAACAAACTGGATGGGACACACCTATTCATGTGGATGCAGCAAGTGGTGGATTCATTGCACCATTTATCTATCCAGAGTTGGAATGGGATTTTAGGCTTCCTTTAGTGAAAAGTATTAATGTGAGTGGACACAAATATGGGCTTGTTTATGCTGGTATTGGTTGGGTTATTTGGAGAACTAAACAAGACTTGCCTCAACAACTCATTTTTCATATCAATTATCTTGGTGCTGATCAGCCTACTTTTACTCTCAATTTCTCTAAAGGTTCAAGTCAAGTCATTGCTCAATATTATCAGCTTATCCGCTTGGGCTATGAGGGATATCGAAATGTAATGGAAAATTGTCGTGAAAATGCAATTGTGCTAAGAAAAGGACTTGAAAAAACAGGACGTTTCAATATAATCTCCAAAGATGAAGGTATACCCTTGGTGGCATTTTCCCTCAAAGACAATAGCCTCCACAACGAATTCGAGGTCTCTGAGACCCTCCGTAGGTTTGGGTGGATTGTCCCAGCCTACACTATGCCAGCTGACCTGCAACATGTTACAGTGTTGCGCGTTGTGATTAGAGAGGACTTCTCCCGAACCCTAGCAGATCGTCTTGTCTCTGACATCGTCAAGGTCCTCCACGAGCTCCCGAATGCCAAAAAAGTGGAGGATAATTTGATGATCAATAATGAGAAGAAAACAGAAATTGAAGTTCAAAGGGCAATTGCTGAGTTTTGGAAGAAATATGTTTTAGCTAGGAAAGCATCTATTTGTTAGGGAAGGATTAATGGTTGTTTTGAAGGAATAAGTATTAATTACTAGTAACGTTTTGGTATTAATTATAAAAAATGTGAAAAGAATTGATGAATGTATCATCCTTTGTTCTATTGAATTGAATATTATATTATGTTACATCTCTTTGTATTATTATGTCTTGTACTGACTTTTGTTAATTTATTTAATTATATTTATTTTTAG
CDS Sequence
- >Solyc01g005000.3.1
ATGGTTCTCTCAAAAACTCCTTCTGATGATTCTGTACACTCCACATTTGCTTCTCGCTATGTTCGAACTTCACTACCAAGGTTTGAGATGCTAGAGAAGTCTATACCAAAAGAGGCAGCATACCAAATGATTAATGATGAGTTAATGCTTGATGGGAATCCAAGGTTAAATTTGGCATCATTTGTAACCACATGGATGGAACCAGAATGTGATAAGCTTATGATGGCTTCAATTAACAAGAATTATGTTGACATGGATGAATACCCTGTCACCACTGAGCTTCAGAATCGATGTGTAAACATGATAGCGCGTTTATTCAATGCGCCTTTGAAAGAGGAAGAAATAGGAATTGGTGTGGGGACAGTGGGGTCATCAGAGGCCATAATGTTAGCAGGGCTGGCCTTCAAGAGGAACTGGCAAAACAAACGCAAAGCTGAGGGAAAGCCTTATGATAAGCCCAACATTGTCACTGGTGCTAATGTTCAGGTGTGTTGGGAGAAATTTGCAAACTACTTTGAAGTGGAATTGAAACAAGTCAAGTTAAGTGAAGGGTACTATGTGATGGACCCAATCAAAGCTGTGGAAATGGTAGATGACAACACTATTTGTGTTGCTGCTATTTTGGGTTCAACACTTAATGGAGAATTTGAAGATGTCAAACTCTTGAATGATCTTTTGATTGAAAAGAATAAACAAACTGGATGGGACACACCTATTCATGTGGATGCAGCAAGTGGTGGATTCATTGCACCATTTATCTATCCAGAGTTGGAATGGGATTTTAGGCTTCCTTTAGTGAAAAGTATTAATGTGAGTGGACACAAATATGGGCTTGTTTATGCTGGTATTGGTTGGGTTATTTGGAGAACTAAACAAGACTTGCCTCAACAACTCATTTTTCATATCAATTATCTTGGTGCTGATCAGCCTACTTTTACTCTCAATTTCTCTAAAGGTTCAAGTCAAGTCATTGCTCAATATTATCAGCTTATCCGCTTGGGCTATGAGGGATATCGAAATGTAATGGAAAATTGTCGTGAAAATGCAATTGTGCTAAGAAAAGGACTTGAAAAAACAGGACGTTTCAATATAATCTCCAAAGATGAAGGTATACCCTTGGTGGCATTTTCCCTCAAAGACAATAGCCTCCACAACGAATTCGAGGTCTCTGAGACCCTCCGTAGGTTTGGGTGGATTGTCCCAGCCTACACTATGCCAGCTGACCTGCAACATGTTACAGTGTTGCGCGTTGTGATTAGAGAGGACTTCTCCCGAACCCTAGCAGATCGTCTTGTCTCTGACATCGTCAAGGTCCTCCACGAGCTCCCGAATGCCAAAAAAGTGGAGGATAATTTGATGATCAATAATGAGAAGAAAACAGAAATTGAAGTTCAAAGGGCAATTGCTGAGTTTTGGAAGAAATATGTTTTAGCTAGGAAAGCATCTATTTGTTAG
Protein Sequence
- >Solyc01g005000.3.1
MVLSKTPSDDSVHSTFASRYVRTSLPRFEMLEKSIPKEAAYQMINDELMLDGNPRLNLASFVTTWMEPECDKLMMASINKNYVDMDEYPVTTELQNRCVNMIARLFNAPLKEEEIGIGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFANYFEVELKQVKLSEGYYVMDPIKAVEMVDDNTICVAAILGSTLNGEFEDVKLLNDLLIEKNKQTGWDTPIHVDAASGGFIAPFIYPELEWDFRLPLVKSINVSGHKYGLVYAGIGWVIWRTKQDLPQQLIFHINYLGADQPTFTLNFSKGSSQVIAQYYQLIRLGYEGYRNVMENCRENAIVLRKGLEKTGRFNIISKDEGIPLVAFSLKDNSLHNEFEVSETLRRFGWIVPAYTMPADLQHVTVLRVVIREDFSRTLADRLVSDIVKVLHELPNAKKVEDNLMINNEKKTEIEVQRAIAEFWKKYVLARKASIC*