Gene Details:

Functional Descriptions:

  • Tomato Glutamate Decarboxylase Genes SlGAD2 and SlGAD3 Play Key Roles in Regulating γ-Aminobutyric Acid Levels in Tomato.
  • We previously identified three tomato genes (SlGAD1, SlGAD2 and SlGAD3) encoding glutamate decarboxylase (GAD), likely the key enzyme for GABA biosynthesis in tomato fruits.
  • A significant decrease in GABA levels, i.e. 50–81% compared with wild-type (WT) levels, was observed in mature green (MG) fruits of the SlGAD2-suppressed lines, while a more drastic reduction (up to <10% of WT levels) was observed in the SlGAD3- and triple SlGAD-suppressed lines.
  • These findings suggest that both SlGAD2 and SlGAD3 expression are crucial for GABA biosynthesis in tomato fruits.
  • We also determined that strong down-regulation of the SlGADs had little effect on overall plant growth, fruit development or primary fruit metabolism under normal growth conditions.

Literature:

Gene Resources:

Orthologs:

Sequences:

cDNA Sequence
  • >Solyc01g005000.3.1
    ATTGGACAACTATAAATAGCCATAGCCCTTTCTCCCAACACACAAACAAACAAACTTTAATTAATTCTCCAAAAACTTTTTAATTATTTACAACAACATAATGGTTCTCTCAAAAACTCCTTCTGATGATTCTGTACACTCCACATTTGCTTCTCGCTATGTTCGAACTTCACTACCAAGGTTTGAGATGCTAGAGAAGTCTATACCAAAAGAGGCAGCATACCAAATGATTAATGATGAGTTAATGCTTGATGGGAATCCAAGGTTAAATTTGGCATCATTTGTAACCACATGGATGGAACCAGAATGTGATAAGCTTATGATGGCTTCAATTAACAAGAATTATGTTGACATGGATGAATACCCTGTCACCACTGAGCTTCAGAATCGATGTGTAAACATGATAGCGCGTTTATTCAATGCGCCTTTGAAAGAGGAAGAAATAGGAATTGGTGTGGGGACAGTGGGGTCATCAGAGGCCATAATGTTAGCAGGGCTGGCCTTCAAGAGGAACTGGCAAAACAAACGCAAAGCTGAGGGAAAGCCTTATGATAAGCCCAACATTGTCACTGGTGCTAATGTTCAGGTGTGTTGGGAGAAATTTGCAAACTACTTTGAAGTGGAATTGAAACAAGTCAAGTTAAGTGAAGGGTACTATGTGATGGACCCAATCAAAGCTGTGGAAATGGTAGATGACAACACTATTTGTGTTGCTGCTATTTTGGGTTCAACACTTAATGGAGAATTTGAAGATGTCAAACTCTTGAATGATCTTTTGATTGAAAAGAATAAACAAACTGGATGGGACACACCTATTCATGTGGATGCAGCAAGTGGTGGATTCATTGCACCATTTATCTATCCAGAGTTGGAATGGGATTTTAGGCTTCCTTTAGTGAAAAGTATTAATGTGAGTGGACACAAATATGGGCTTGTTTATGCTGGTATTGGTTGGGTTATTTGGAGAACTAAACAAGACTTGCCTCAACAACTCATTTTTCATATCAATTATCTTGGTGCTGATCAGCCTACTTTTACTCTCAATTTCTCTAAAGGTTCAAGTCAAGTCATTGCTCAATATTATCAGCTTATCCGCTTGGGCTATGAGGGATATCGAAATGTAATGGAAAATTGTCGTGAAAATGCAATTGTGCTAAGAAAAGGACTTGAAAAAACAGGACGTTTCAATATAATCTCCAAAGATGAAGGTATACCCTTGGTGGCATTTTCCCTCAAAGACAATAGCCTCCACAACGAATTCGAGGTCTCTGAGACCCTCCGTAGGTTTGGGTGGATTGTCCCAGCCTACACTATGCCAGCTGACCTGCAACATGTTACAGTGTTGCGCGTTGTGATTAGAGAGGACTTCTCCCGAACCCTAGCAGATCGTCTTGTCTCTGACATCGTCAAGGTCCTCCACGAGCTCCCGAATGCCAAAAAAGTGGAGGATAATTTGATGATCAATAATGAGAAGAAAACAGAAATTGAAGTTCAAAGGGCAATTGCTGAGTTTTGGAAGAAATATGTTTTAGCTAGGAAAGCATCTATTTGTTAGGGAAGGATTAATGGTTGTTTTGAAGGAATAAGTATTAATTACTAGTAACGTTTTGGTATTAATTATAAAAAATGTGAAAAGAATTGATGAATGTATCATCCTTTGTTCTATTGAATTGAATATTATATTATGTTACATCTCTTTGTATTATTATGTCTTGTACTGACTTTTGTTAATTTATTTAATTATATTTATTTTTAG
CDS Sequence
  • >Solyc01g005000.3.1
    ATGGTTCTCTCAAAAACTCCTTCTGATGATTCTGTACACTCCACATTTGCTTCTCGCTATGTTCGAACTTCACTACCAAGGTTTGAGATGCTAGAGAAGTCTATACCAAAAGAGGCAGCATACCAAATGATTAATGATGAGTTAATGCTTGATGGGAATCCAAGGTTAAATTTGGCATCATTTGTAACCACATGGATGGAACCAGAATGTGATAAGCTTATGATGGCTTCAATTAACAAGAATTATGTTGACATGGATGAATACCCTGTCACCACTGAGCTTCAGAATCGATGTGTAAACATGATAGCGCGTTTATTCAATGCGCCTTTGAAAGAGGAAGAAATAGGAATTGGTGTGGGGACAGTGGGGTCATCAGAGGCCATAATGTTAGCAGGGCTGGCCTTCAAGAGGAACTGGCAAAACAAACGCAAAGCTGAGGGAAAGCCTTATGATAAGCCCAACATTGTCACTGGTGCTAATGTTCAGGTGTGTTGGGAGAAATTTGCAAACTACTTTGAAGTGGAATTGAAACAAGTCAAGTTAAGTGAAGGGTACTATGTGATGGACCCAATCAAAGCTGTGGAAATGGTAGATGACAACACTATTTGTGTTGCTGCTATTTTGGGTTCAACACTTAATGGAGAATTTGAAGATGTCAAACTCTTGAATGATCTTTTGATTGAAAAGAATAAACAAACTGGATGGGACACACCTATTCATGTGGATGCAGCAAGTGGTGGATTCATTGCACCATTTATCTATCCAGAGTTGGAATGGGATTTTAGGCTTCCTTTAGTGAAAAGTATTAATGTGAGTGGACACAAATATGGGCTTGTTTATGCTGGTATTGGTTGGGTTATTTGGAGAACTAAACAAGACTTGCCTCAACAACTCATTTTTCATATCAATTATCTTGGTGCTGATCAGCCTACTTTTACTCTCAATTTCTCTAAAGGTTCAAGTCAAGTCATTGCTCAATATTATCAGCTTATCCGCTTGGGCTATGAGGGATATCGAAATGTAATGGAAAATTGTCGTGAAAATGCAATTGTGCTAAGAAAAGGACTTGAAAAAACAGGACGTTTCAATATAATCTCCAAAGATGAAGGTATACCCTTGGTGGCATTTTCCCTCAAAGACAATAGCCTCCACAACGAATTCGAGGTCTCTGAGACCCTCCGTAGGTTTGGGTGGATTGTCCCAGCCTACACTATGCCAGCTGACCTGCAACATGTTACAGTGTTGCGCGTTGTGATTAGAGAGGACTTCTCCCGAACCCTAGCAGATCGTCTTGTCTCTGACATCGTCAAGGTCCTCCACGAGCTCCCGAATGCCAAAAAAGTGGAGGATAATTTGATGATCAATAATGAGAAGAAAACAGAAATTGAAGTTCAAAGGGCAATTGCTGAGTTTTGGAAGAAATATGTTTTAGCTAGGAAAGCATCTATTTGTTAG
Protein Sequence
  • >Solyc01g005000.3.1
    MVLSKTPSDDSVHSTFASRYVRTSLPRFEMLEKSIPKEAAYQMINDELMLDGNPRLNLASFVTTWMEPECDKLMMASINKNYVDMDEYPVTTELQNRCVNMIARLFNAPLKEEEIGIGVGTVGSSEAIMLAGLAFKRNWQNKRKAEGKPYDKPNIVTGANVQVCWEKFANYFEVELKQVKLSEGYYVMDPIKAVEMVDDNTICVAAILGSTLNGEFEDVKLLNDLLIEKNKQTGWDTPIHVDAASGGFIAPFIYPELEWDFRLPLVKSINVSGHKYGLVYAGIGWVIWRTKQDLPQQLIFHINYLGADQPTFTLNFSKGSSQVIAQYYQLIRLGYEGYRNVMENCRENAIVLRKGLEKTGRFNIISKDEGIPLVAFSLKDNSLHNEFEVSETLRRFGWIVPAYTMPADLQHVTVLRVVIREDFSRTLADRLVSDIVKVLHELPNAKKVEDNLMINNEKKTEIEVQRAIAEFWKKYVLARKASIC*